Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,512,543 G→T 5.9% intergenic (‑71/‑42) EO946_RS07905 ← / → typA YlaF family protein/translational GTPase TypA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,512,5430GT 5.9% 503.0 / 4.7 168intergenic (‑71/‑42)EO946_RS07905/typAYlaF family protein/translational GTPase TypA
Reads supporting (aligned to +/- strand):  ref base G (114/41);  new base T (2/11);  total (116/52)
Fisher's exact test for biased strand distribution p-value = 5.05e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 171 total aligned reads displayed.

CGATCAGCATAATACTGAAAACCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCTTTTACACCAGGCTGGT  >  NZ_CP034943/1512406‑1512680
                                                                                                                                         |                                                                                                                                         
cGATCAGCATAATACTGAAAACCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGta                                                                                                                                         <  2:1127735/140‑1 (MQ=255)
cGATCAGCATAATACTGAAAACCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGta                                                                                                                                         <  2:4108239/140‑1 (MQ=255)
 gATCAGCATAATACTGAAAACCTCTGCAAATGCAAATAATAGTACAAGCCAATTCATTTATGTCATATTTTCCATCCCCTTTTACGTCTTATCATCACTTTACAGAAAAACGTGACGCTTGTAAAGAGGATGTTTGGtat                                                                                                                                        >  1:4045045/1‑140 (MQ=255)
       cATAATACTGAAAACCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAAtgtg                                                                                                                                  >  1:826756/1‑140 (MQ=255)
          cataCTGAAAACCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCCTATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTAGAGACAAACGTGACGCTTTTAGAGCGGATGTGTGGTAGAATGTGaaa                                                                                                                               >  2:2523495/2‑140 (MQ=255)
          aataCTGAAAACCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTTCGTCTTCTCCTAAGTTTACAGAAAAACGTGACGCTTTTAAAGTGGATGTGTGGTATACTGTGaaa                                                                                                                               >  1:3225290/1‑140 (MQ=255)
          aataCTGAAAACCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAATTTTACAGAAAAACGTGACGATTTTAAAGAGGATGTGTGGTATAATGTGaaa                                                                                                                               >  1:1774731/1‑140 (MQ=255)
            taCTGAAAACCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTGTTACGTCTTATCATAAGTTTACAGAAAATCGTGACGCTTTTAATGAGGATGTGTGGTATAATGTGAAAgg                                                                                                                             >  1:3456290/1‑139 (MQ=255)
               tGAAAACCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTATACGTCGTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTAt                                                                                                                          >  2:3526186/1‑140 (MQ=255)
                gAAAACCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCACTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATc                                                                                                                         >  1:47234/1‑140 (MQ=255)
                 aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACATAAAAACGTGACGCTTTTAAAGAGTATGTGTGGTATAATGTGAAAGTTATCt                                                                                                                        >  1:4154865/1‑140 (MQ=255)
                 aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCt                                                                                                                        >  2:2082328/1‑140 (MQ=255)
                 aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCt                                                                                                                        >  2:2449923/1‑140 (MQ=255)
                 aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCt                                                                                                                        >  1:3891023/1‑140 (MQ=255)
                 aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCt                                                                                                                        >  2:4074316/1‑140 (MQ=255)
                 aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCt                                                                                                                        >  1:4009252/1‑140 (MQ=255)
                 aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCt                                                                                                                        >  2:3072515/1‑140 (MQ=255)
                 aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGAGGTGTGGTATCATGTGAAAGTTATCt                                                                                                                        >  1:1178680/1‑140 (MQ=255)
                 aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTGATCATACGTGTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCt                                                                                                                        >  1:2160280/1‑140 (MQ=255)
                 aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCAATCACCTTTTACGTCTTATCATAAGTTTACAGAAAAAATTGACGCTTGTAAAGAGGATGTGTGTTATAATTTGAAAGTTATCt                                                                                                                        >  1:1109855/1‑140 (MQ=255)
                      cGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTCTAATGTGAAAGTTATCTAAttt                                                                                                                   >  1:902123/1‑140 (MQ=255)
                        cTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTCTAATGTGAAAGTTATCTAATTtta                                                                                                                 >  2:2133627/1‑140 (MQ=255)
                        cTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTtta                                                                                                                 >  2:1604864/1‑140 (MQ=255)
                                   aataatAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGa                                                                                                      <  1:3097170/140‑1 (MQ=255)
                                           tAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGa                                                                                              >  1:4278545/1‑140 (MQ=255)
                                           tAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGa                                                                                              >  1:2903007/1‑140 (MQ=255)
                                           tAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGa                                                                                              >  1:77245/1‑140 (MQ=255)
                                              aaGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGaaag                                                                                           >  1:2573593/1‑139 (MQ=255)
                                              aaGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAAc                                                                                           >  1:466245/1‑140 (MQ=255)
                                                gCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTCTAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACtt                                                                                         >  2:4126815/1‑140 (MQ=255)
                                                gCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACtt                                                                                         >  2:583655/1‑140 (MQ=255)
                                                gCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACtt                                                                                         >  2:3402147/1‑140 (MQ=255)
                                                     ttCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAATAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGaaa                                                                                    >  2:1673594/1‑140 (MQ=255)
                                                     ttCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATTTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGaaa                                                                                    >  2:1295445/1‑140 (MQ=255)
                                                     ttCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGaaa                                                                                    >  1:4321376/1‑140 (MQ=255)
                                                       cATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTTTGGTATAATTTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATg                                                                                  <  2:3397919/140‑1 (MQ=255)
                                                       cATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATg                                                                                  <  1:169838/140‑1 (MQ=255)
                                                       cATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATg                                                                                  <  2:104399/140‑1 (MQ=255)
                                                       cATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATg                                                                                  <  1:1345218/140‑1 (MQ=255)
                                                       cATTTTTTTCATATTTTCCATCCCCTTTTACGTATTATATTAAGTTTACAGTATAACGTTACTCTTTTAAAGAGGATTTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATg                                                                                  <  2:3178733/140‑1 (MQ=255)
                                                         tttttttCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGAt                                                                                <  1:1830106/140‑1 (MQ=255)
                                                         tttttttCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGAt                                                                                <  1:987352/140‑1 (MQ=255)
                                                         cttttttCATATTTTCCCTCCCCAATAACCACTGATCATAAGTTTACACAAAAACGTGACGCTGTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGAt                                                                                <  1:4304127/139‑1 (MQ=255)
                                                          ttttttCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAATTGAAACTTCGAAATGATc                                                                               >  2:2420634/1‑140 (MQ=255)
                                                                cATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTAAAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCa                                                                         <  1:3839265/140‑1 (MQ=255)
                                                                    ttttCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACag                                                                     <  2:497669/140‑2 (MQ=255)
                                                                    ttttCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACAt                                                                     <  2:1818498/140‑1 (MQ=255)
                                                                    ttttCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACAt                                                                     <  2:902123/140‑1 (MQ=255)
                                                                          aTCACCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGat                                                               <  2:26808/140‑1 (MQ=255)
                                                                           tCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGatt                                                              <  1:25681/140‑1 (MQ=255)
                                                                               cTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTg                                                          <  1:1508914/140‑1 (MQ=255)
                                                                                ttttACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGc                                                         <  2:1735587/140‑1 (MQ=255)
                                                                                ttttACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGc                                                         <  1:407118/140‑1 (MQ=255)
                                                                                 tttACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGcc                                                        <  2:3891023/140‑1 (MQ=255)
                                                                                 tttACGTATTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGATGATTTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGcc                                                        <  2:2011368/140‑1 (MQ=255)
                                                                                     cGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACg                                                    <  1:1964096/140‑1 (MQ=255)
                                                                                       tCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGtt                                                  >  1:485345/1‑140 (MQ=255)
                                                                                        cTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTg                                                 >  1:1070222/1‑140 (MQ=255)
                                                                                         ttATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGa                                                >  2:859521/1‑140 (MQ=255)
                                                                                         ttATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGa                                                >  2:373265/1‑140 (MQ=255)
                                                                                         ttATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGa                                                >  1:4020725/1‑140 (MQ=255)
                                                                                          tATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGAc                                               >  1:2558383/1‑140 (MQ=255)
                                                                                          tATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGAc                                               >  1:1853478/1‑140 (MQ=255)
                                                                                          tATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGAc                                               >  1:1538574/1‑140 (MQ=255)
                                                                                          tATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACATCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGATGAc                                               >  1:30771/1‑140 (MQ=255)
                                                                                             cATAAGTTTACATAAAAACGTGACTCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCAt                                            <  2:4025774/140‑1 (MQ=255)
                                                                                             cATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCAt                                            <  2:817999/140‑1 (MQ=255)
                                                                                                 aGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAAGTTTATTAGGAGATGAAAAAGGGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACGCTGGGa                                        >  1:2621107/1‑140 (MQ=255)
                                                                                                 aGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTAACTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATTATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGa                                        >  1:2216692/1‑140 (MQ=255)
                                                                                                     tACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAAc                                    >  1:3033891/1‑140 (MQ=255)
                                                                                                     tACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAAc                                    >  1:965518/1‑140 (MQ=255)
                                                                                                      aCAGAAAAACGTGACGCTTTTAAAGAGGATGGGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACg                                   >  1:2136500/1‑140 (MQ=255)
                                                                                                           aaaaCGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGattat                                                            >  1:1265795/1‑110 (MQ=255)
                                                                                                           aaaaCGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGattat                                                            <  2:1265795/110‑1 (MQ=255)
                                                                                                           aaaaCGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGattat                                                            >  1:2825232/1‑110 (MQ=255)
                                                                                                           aaaaCGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGattat                                                            <  2:2825232/110‑1 (MQ=255)
                                                                                                            aaaCGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTa                             >  1:1215162/1‑140 (MQ=255)
                                                                                                            aaaCGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGGTGACCATGGGAAAACGACTCTa                             >  1:4302629/1‑140 (MQ=255)
                                                                                                             aaCGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAg                            >  1:1409964/1‑140 (MQ=255)
                                                                                                                   tctattttAAAGAGTTTGTGTGTGATATTGTTAAATTTATCTAATTTGATTTGGAGTTTAAAATGTCAAACTTCGAATTGATCTTCGCAACAGCGCGTTTTGGGCCAACGTTGACCATGGGAAAACGACTCTAGTCGATc                      <  2:3973919/136‑1 (MQ=255)
                                                                                                                   acttttttAATTAGGTTGTTTGTTATATTTTTAATGTTATCTTTTTTTATTTGTATATTAAAAAGTTAAACTTGTAAATGATTTTCCCAACACCGCTTTTTTTGCCCAGGTGGACCATGGGAAAACGACTCTAGTCGATc                      <  2:2900037/136‑1 (MQ=255)
                                                                                                                   aCGCTTTTAAAGAGGAGGTGTGTTTTACTGTGAATTTTATCTAATTTTATTAGGAGATTAAAAAGTTAAACTTCTAAATCATCTACGCAACATCGCTATTATTGCCCACGTTCACCTTGGTAACACGACTCTAGTCGATc                      <  1:696730/140‑1 (MQ=255)
                                                                                                                    cGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCa                     >  1:626863/1‑140 (MQ=255)
                                                                                                                    cGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCa                     >  1:3282400/1‑140 (MQ=255)
                                                                                                                    cGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCa                     >  1:4160729/1‑140 (MQ=255)
                                                                                                                              gtgtttgtttgttATATTGTTAAATTTATCTATTTTTATTAGGATATTAAAAAGTTAAATTTCGAAATGATATTCGCAACATCGCTTTTTTTGCCCACTTTGACCTGGGCAACACGACTCTAGTCGATCAGCTTTTacac           <  1:557884/135‑1 (MQ=255)
                                                                                                                              gtgttggtgtgtTATATTTTGAATGTAATATAATTTTATTAGGTGATTAAAAATTTAAACTTCTAAATGATCTTCGCAACATCGCTTTTATTGCCCCCGTGGACCATGGCAAAACGACTCTAGTCGATCAGCTTTTacac           <  2:826756/134‑1 (MQ=255)
                                                                                                                              gcggATGTGTGTTATTATGTCAATGTTATCTAATTTTATGACGAGATTAAAAAGTCAAACTTCTAATTGATCTTCGAACCAGCGCGTTTTTTCCCCCGGTGGACCATGCTAAAACGACTCTAGTCGATCAGCTTTTacac           <  2:2926367/138‑1 (MQ=255)
                                                                                                                                 gATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGAGTCTAGTCGATCAGCTTTTAcagcag        >  1:3605066/1‑140 (MQ=255)
                                                                                                                                 gATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTATTCTATCAGCTTTTACACCAg        >  1:2075038/1‑140 (MQ=255)
                                                                                                                                 gATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCTTTTACACCAg        >  1:4097099/1‑140 (MQ=255)
                                                                                                                                 gATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCTTTTACACCAg        >  1:1091837/1‑140 (MQ=255)
                                                                                                                                 gATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCTTTTACACCAg        >  1:1687212/1‑140 (MQ=255)
                                                                                                                                 gATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCTTTTACACCAg        >  1:2993238/1‑140 (MQ=255)
                                                                                                                                 gATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCTTTTACACCAg        >  2:2745196/1‑140 (MQ=255)
                                                                                                                                 gATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATAAGCTTTTACACCAg        >  1:438873/1‑140 (MQ=255)
                                                                                                                                  ttgtgttGTATAATTTTAAATTTATATAATTTTTTTAGTAGTTGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCTTTTACACCAgg       <  2:4278545/139‑1 (MQ=255)
                                                                                                                                  ttgtgtgTTATATTGTTAATTTTATATATTTTTTTTAGGAGATTAAAAATTTAAACTTCTAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTCACCATGGTAAAACGACTCTAGTCGATCAGCTTTTACACCAgg       <  2:77258/139‑1 (MQ=255)
                                                                                                                                  ttgtgtgTTATAATTTTAATTTTATCTATTTTTATTAGGAGATTAAAAAGTTAAAATTCGAATTGATCTTCCCAACATCGCTTTTTTTGCCCAGGTTGACCATGGTAAAACGACTCTAGTCGATCAGCTTTTACACCAgg       <  1:787784/139‑1 (MQ=255)
                                                                                                                                  aTGTGTGTTTTAATGTTAAATTTATATATTTTTTTTAGGATTTGAAAATGTGAAACTTCGAATTGATGTTCGAAACATCGCTTTTTTTGCCCACTTTGACCTTGGGAAAACGACTCTAGTCGATCAGCTTTTACACCAgg       <  2:2178646/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                         
CGATCAGCATAATACTGAAAACCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCTTTTACACCAGGCTGGT  >  NZ_CP034943/1512406‑1512680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: