Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,570,501 A→T 5.6% A62A (GCA→GCT spoIIGA → sigma‑E processing peptidase SpoIIGA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,570,5010AT 5.6% 387.0 / 6.8 119A62A (GCA→GCTspoIIGAsigma‑E processing peptidase SpoIIGA
Reads supporting (aligned to +/- strand):  ref base A (46/65);  new base T (3/4);  total (50/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

ATGCACTTTTGCTTTTGCTCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGGGAGGAGGAATTATCGGGGCCCA  >  NZ_CP034943/1570362‑1570632
                                                                                                                                           |                                                                                                                                   
aTGCACTTTTGCTTTTGCTCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCa                                                                                                                                     <  1:3988878/140‑1 (MQ=255)
aTGCACTTTTGCTTTTGCTCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTACAGTATCGTGCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCa                                                                                                                                     <  1:2260775/140‑1 (MQ=255)
      ttttgcttttgcTCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAg                                                                                                                               <  1:289610/140‑1 (MQ=255)
      ttttgcttttgcTCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAg                                                                                                                               <  2:1213788/140‑1 (MQ=255)
      ttttgcttttgcTCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAg                                                                                                                               <  1:1974888/140‑1 (MQ=255)
      ttttgcttttgcTCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAg                                                                                                                               <  2:2116516/140‑1 (MQ=255)
      ttttgcttttgcTCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAg                                                                                                                               <  1:196111/140‑1 (MQ=255)
      ttttgcttttgcTCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAg                                                                                                                               <  1:196093/140‑1 (MQ=255)
           cttttgctCACGGCATTTATTTTAAAACGGCATGTGAAAAAAACAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGc                                                                                                                          <  1:1545503/140‑1 (MQ=255)
              ttgctCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCttt                                                                                                                       <  2:1455888/140‑1 (MQ=255)
              ttgctCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCttt                                                                                                                       <  2:1516020/140‑1 (MQ=255)
              ttgctCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCttt                                                                                                                       >  2:319489/1‑140 (MQ=255)
                gctCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCttttt                                                                                                                     >  2:911369/1‑140 (MQ=255)
                gctCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCttttt                                                                                                                     >  2:3819488/1‑140 (MQ=255)
                gctCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCttttt                                                                                                                     >  2:4213527/1‑140 (MQ=255)
                gctCACGGCATTTATTTTAAAACGGCATGTAAAAAAAAGAAGACTGGTAGGCGGAGCGTTCATCGGTTCCAGTATTGTCCTTTTGATGTTTACTCCTTTTTCGCCGATTGTTGAACATCCGGCTGGGAAGCTAGCttttt                                                                                                                     >  1:3549298/1‑140 (MQ=255)
                gctCACGGCATTTATTTTAAAACGGCATGTAAAAAAAAGAAGACTGGTAGGCGGAGCGTTCATCGGTTCCAGTATTGTCCTTTTGATGTTTACTCCTTTTTCGCCGATTGTTGAACATCCGGCTGGGAAGCTAGCttttt                                                                                                                     >  1:3548947/1‑140 (MQ=255)
                gctCACGGCATTTATTTTAAAACGGCATGTAAAAAAAAGAAGACTGGTAGGCGGAGCGTTCATCGGTTCCAGTATTGTCCTTTTGATGTTTACTCCTGTTTCGCCGATTGTTGAACATCCGGCTGGGAAGCTAGCttttt                                                                                                                     >  1:3548819/1‑140 (MQ=255)
                 ctCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTc                                                                                                                    <  2:1580138/140‑1 (MQ=255)
                 ctCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTc                                                                                                                    <  2:3052072/140‑1 (MQ=255)
                 ctCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTc                                                                                                                    <  2:3695995/140‑1 (MQ=255)
                 ctCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTc                                                                                                                    <  2:1274982/140‑1 (MQ=255)
                 ctCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTc                                                                                                                    <  2:4102441/140‑1 (MQ=255)
                 ctCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACCCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTc                                                                                                                    <  2:3974831/140‑1 (MQ=255)
                 ctCACGGCATTTATGTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTc                                                                                                                    <  2:1085471/140‑1 (MQ=255)
                   cACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAg                                                                                                                  <  1:2117524/140‑1 (MQ=255)
                   cACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAg                                                                                                                  <  2:1346462/140‑1 (MQ=255)
                    aCGGCATTTATTTTAAACCGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAgt                                                                                                                 <  2:3992821/140‑1 (MQ=255)
                    aCGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGATCCTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAgt                                                                                                                 <  2:4065437/140‑1 (MQ=255)
                             atttTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATtgt                                                                                                        <  1:491971/140‑1 (MQ=255)
                              ttttAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATtgtg                                                                                                       <  2:190962/140‑1 (MQ=255)
                                ttAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCATTTGTTATTGTGAt                                                                                                     >  1:52669/1‑140 (MQ=255)
                                ttAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGAt                                                                                                     >  1:3562016/1‑140 (MQ=255)
                                ttAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGAt                                                                                                     >  1:3562098/1‑140 (MQ=255)
                                  aaaaCGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATgg                                                                                                   <  1:1353057/140‑1 (MQ=255)
                                   aaaCGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGt                                                                                                  <  2:2131050/140‑1 (MQ=255)
                                   aaaCGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGt                                                                                                  <  2:23760/140‑1 (MQ=255)
                                   aaaCGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGt                                                                                                  <  2:3575774/140‑1 (MQ=255)
                                   aaaCGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAAAATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGt                                                                                                  <  2:73333/140‑1 (MQ=255)
                                        gCATGTGAAAAAAAGAAGACTGCTAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTGCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACAt                                                                                             <  2:4150317/140‑1 (MQ=255)
                                        gCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACAt                                                                                             <  1:319489/140‑1 (MQ=255)
                                                      gaagaCTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAgaga                                                                               <  1:3376684/140‑1 (MQ=255)
                                                      gaagaCTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAgaga                                                                               <  1:3002928/140‑1 (MQ=255)
                                                      gaagaCTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAgaga                                                                               <  1:1392072/140‑1 (MQ=255)
                                                      gaagaCTGATAGGCGGAGCGGTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAgaga                                                                               <  1:731549/140‑1 (MQ=255)
                                                                 ggCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTAttt                                                                    >  2:2311100/1‑140 (MQ=255)
                                                                   cGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACAttt                                                                                           <  1:1011751/115‑1 (MQ=255)
                                                                     gAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTAtttcttt                                                                >  2:1247467/1‑140 (MQ=255)
                                                                      aGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCGGGTAAGCTGGCGTTTTCCGGTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTCtttctttc                                                               >  2:489478/1‑140 (MQ=255)
                                                                      aGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTAtttctttc                                                               >  1:534182/1‑140 (MQ=255)
                                                                      aGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTAtttctttc                                                               >  1:1580145/1‑140 (MQ=255)
                                                                      aGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATAGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCAGCTAtttctttc                                                               >  2:1076980/1‑140 (MQ=255)
                                                                          ttCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCaaaa                                                           >  2:2976507/1‑140 (MQ=255)
                                                                          ttCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCaaaa                                                           >  2:3508481/1‑140 (MQ=255)
                                                                          ttCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCaaaa                                                           >  2:3775524/1‑140 (MQ=255)
                                                                                  ttCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTc                                                                                                                    >  1:2817300/1‑75 (MQ=255)
                                                                                  ttCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTc                                                                                                                    <  2:2817300/75‑1 (MQ=255)
                                                                                            gTCCTTTTGATGTTTACTCCTTTTTCGCCGCTCGTGGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATGGTGATGGTGAGATGTGGCTTTAAGAGATTCCGCTATTTCTTTCACAATTTGTTTGACGTTTAtgt                                         >  2:2474017/1‑138 (MQ=255)
                                                                                            gTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACCGTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCGTTTTAgtt                                         >  1:2941688/1‑140 (MQ=255)
                                                                                            gTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACAtt                                                                                            >  2:3343221/1‑89 (MQ=255)
                                                                                            gTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACAtt                                                                                            <  1:3343221/89‑1 (MQ=255)
                                                                                            gTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACAt                                                                                             <  1:1061093/88‑1 (MQ=255)
                                                                                            gTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACAt                                                                                             >  2:1061093/1‑88 (MQ=255)
                                                                                            gTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTAttt                                         >  2:809019/1‑140 (MQ=255)
                                                                                            gTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTAttt                                         >  2:4246793/1‑140 (MQ=255)
                                                                                            gTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTAttt                                         >  2:1445131/1‑140 (MQ=255)
                                                                                            gTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTAttt                                         >  2:4230305/1‑140 (MQ=255)
                                                                                            gTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTAttt                                         >  1:2919497/1‑140 (MQ=255)
                                                                                            gTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTAttt                                         >  2:1123821/1‑140 (MQ=255)
                                                                                             tCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTAtttt                                        >  1:3087722/1‑140 (MQ=255)
                                                                                                  ttGATGTTTACTCCTTTTTCGCCTATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCCGTTGTTATTGTGATGGTGACATTTGGCTGTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGAc                                   >  2:599808/1‑140 (MQ=255)
                                                                                                  ttGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGAc                                   >  1:2348864/1‑140 (MQ=255)
                                                                                                   ttatttttACTGATTTTTCGCCTTTCGTTGAACATGCGGCATGTAAGCTGGCTTTTTCAGTTTTTATTTTGATGTTTAATTGTGGCTTTAAGCTATTCCGATATTTCTTTCTATTTTTGTTTTCATTTTTTTTTGCGata                                  <  2:2470577/135‑3 (MQ=255)
                                                                                                   ttaTGTTTACTCCATTTTCGGCTATCTTTGAACATCCGGCTTGTAAGCTGTCTTTTTCAGTTGTTATTGTTATGTTGACATTTGGCTTTAAGATATTCCGCTATTTATTTCAAAATTTGTTTTCCTTTTATTTTGCGaat                                  <  2:3528015/138‑3 (MQ=255)
                                                                                                   tGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTGTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACt                                  <  2:3573793/140‑1 (MQ=255)
                                                                                                       gTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACttttt                              >  2:2649415/1‑140 (MQ=255)
                                                                                                          tACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTaa                           <  2:3020363/140‑1 (MQ=255)
                                                                                                           aCTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAAt                          <  1:911369/140‑1 (MQ=255)
                                                                                                           aCTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAAt                          <  1:821717/140‑1 (MQ=255)
                                                                                                           aCTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAAt                          <  1:4213527/140‑1 (MQ=255)
                                                                                                           aCTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAAt                          <  1:1229468/140‑1 (MQ=255)
                                                                                                           aCTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAAt                          <  1:3894891/140‑1 (MQ=255)
                                                                                                           aCTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAAt                          <  1:3819488/140‑1 (MQ=255)
                                                                                                           aCTCCTTTTTCGCCGATCGTTGAACATCCGCCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAAt                          <  1:1937255/140‑1 (MQ=255)
                                                                                                           aCTCCTATTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGTTGACATTTTGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTTTTTTGCGACTTTTTTAAt                          <  1:1932817/140‑1 (MQ=255)
                                                                                                                tttttCGCCGATTGTTGAACATCCGGCTGGGAAGCTAGCTTTTTCAGTTGTGATTGTGGTGGTGACATTTGGCTTTAAAAGATTTCGGTTTTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCCACTTTTTTAATggggg                     <  2:3548819/140‑3 (MQ=255)
                                                                                                                tttttCGCCGATTGTTGAACATCCGGCTGGGAAGCTAGCTTTTTCAGTTGTGATTGTGGTGGTGACATTTGGCTTTAAAAGATTTCGGTTTTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCCACTTTTTTAATggggg                     <  2:3548947/140‑3 (MQ=255)
                                                                                                                 ttttCGCCGATCGTTGAACATCCGGCATGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGggagg                    <  1:1718953/140‑1 (MQ=255)
                                                                                                                 ttttCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGggagg                    <  1:833078/140‑1 (MQ=255)
                                                                                                                  tttCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGggagga                   >  1:2532011/1‑140 (MQ=255)
                                                                                                                  tttCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGggagga                   >  1:843158/1‑140 (MQ=255)
                                                                                                                  tttCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTGTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGAGTTTTTTAATGggagga                   >  2:2131753/1‑140 (MQ=255)
                                                                                                                  tttCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGAAATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGtgagga                   >  1:1708063/1‑140 (MQ=255)
                                                                                                                           tCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGGGAGGAGGAATTATc          <  1:3087227/140‑1 (MQ=255)
                                                                                                                                gaccATCCGGCAGGTACGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGGTTTCCTTTTATTTTGCGACTTTTTTAATGGGAGGAGGAATTATCGGGGc     <  2:2075442/137‑1 (MQ=255)
                                                                                                                                gAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGGGAGGAGGAATTATCGGGGc     <  1:1329625/140‑1 (MQ=255)
                                                                                                                                gAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGGGAGGAGGAATTATCGGGGc     <  1:4051411/140‑1 (MQ=255)
                                                                                                                                   cATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGGGAGGAGGAATTATCGGGGCCCa  <  2:217502/140‑1 (MQ=255)
                                                                                                                                   cATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGAGGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGGGAGGAGGAATTATCGGGGCCCa  <  2:4223407/140‑1 (MQ=255)
                                                                                                                                   cATCCGGCAGGTAAGCTGGCATTTTCAGTGGGTATTGTGATCGTGACATTTGGCTTTAAGCCAGTCCGCTATTTCATTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGGGAGGAGGAATTATCGGGGCCCa  <  1:376228/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                   
ATGCACTTTTGCTTTTGCTCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGGGAGGAGGAATTATCGGGGCCCA  >  NZ_CP034943/1570362‑1570632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: