Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,570,504 T→G 5.2% G63G (GGT→GGG spoIIGA → sigma‑E processing peptidase SpoIIGA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,570,5040TG 5.2% 386.6 / 5.6 122G63G (GGT→GGGspoIIGAsigma‑E processing peptidase SpoIIGA
Reads supporting (aligned to +/- strand):  ref base T (47/68);  new base G (3/4);  total (50/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

TTTTGCTTTTGCTCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGGGAGGAGGAATTATCGGGGCCCATTCTTTGCTG  >  NZ_CP034943/1570368‑1570642
                                                                                                                                        |                                                                                                                                          
ttttgcttttgcTCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAg                                                                                                                                         <  1:289610/140‑1 (MQ=255)
ttttgcttttgcTCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAg                                                                                                                                         <  2:1213788/140‑1 (MQ=255)
ttttgcttttgcTCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAg                                                                                                                                         <  1:1974888/140‑1 (MQ=255)
ttttgcttttgcTCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAg                                                                                                                                         <  2:3878773/140‑1 (MQ=255)
ttttgcttttgcTCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAg                                                                                                                                         <  1:196111/140‑1 (MQ=255)
ttttgcttttgcTCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAg                                                                                                                                         <  2:2116516/140‑1 (MQ=255)
ccttgcttgtgctCACGGCATGTATTTTAAAACGGCATCTGAAAAAAAGAAGACTGAGAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAg                                                                                                                                         <  1:2828104/138‑1 (MQ=255)
     cttttgctCACGGCATTTATTTTAAAACGGCATGTGAAAAAAACAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGc                                                                                                                                    <  1:1545503/140‑1 (MQ=255)
        ttgctCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCttt                                                                                                                                 >  2:319489/1‑140 (MQ=255)
        ttgctCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCttt                                                                                                                                 <  2:1516020/140‑1 (MQ=255)
        ttgctCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCttt                                                                                                                                 <  2:1455888/140‑1 (MQ=255)
          gctCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCttttt                                                                                                                               >  2:1229468/1‑140 (MQ=255)
          gctCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCttttt                                                                                                                               >  2:3819488/1‑140 (MQ=255)
          gctCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCttttt                                                                                                                               >  2:4213527/1‑140 (MQ=255)
          gctCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCttttt                                                                                                                               >  2:911369/1‑140 (MQ=255)
          gctCACGGCATTTATTTTAAAACGGCATGTAAAAAAAAGAAGACTGGTAGGCGGAGCGTTCATCGGTTCCAGTATTGTCCTTTTGATGTTTACTCCTTTTTCGCCGATTGTTGAACATCCGGCTGGGAAGCTAGCttttt                                                                                                                               >  1:3549298/1‑140 (MQ=255)
          gctCACGGCATTTATTTTAAAACGGCATGTAAAAAAAAGAAGACTGGTAGGCGGAGCGTTCATCGGTTCCAGTATTGTCCTTTTGATGTTTACTCCTGTTTCGCCGATTGTTGAACATCCGGCTGGGAAGCTAGCttttt                                                                                                                               >  1:3548819/1‑140 (MQ=255)
           ctCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTc                                                                                                                              <  2:3052072/140‑1 (MQ=255)
           ctCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTc                                                                                                                              <  2:3695995/140‑1 (MQ=255)
           ctCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTc                                                                                                                              <  2:4102441/140‑1 (MQ=255)
           ctCACGGCATTTATGTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTc                                                                                                                              <  2:1085471/140‑1 (MQ=255)
             cACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAg                                                                                                                            <  2:1346462/140‑1 (MQ=255)
             cACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAg                                                                                                                            <  1:2117524/140‑1 (MQ=255)
              aCGGCATTTATTTTAAACCGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAgt                                                                                                                           <  2:3992821/140‑1 (MQ=255)
              aCGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGATCCTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAgt                                                                                                                           <  2:4065437/140‑1 (MQ=255)
                       atttTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATtgt                                                                                                                  <  1:701079/140‑1 (MQ=255)
                        ttttAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATtgtg                                                                                                                 <  2:190962/140‑1 (MQ=255)
                          ttAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCATTTGTTATTGTGAt                                                                                                               >  1:52669/1‑140 (MQ=255)
                          ttAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGAt                                                                                                               >  1:3562016/1‑140 (MQ=255)
                          ttAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGAt                                                                                                               >  1:3562098/1‑140 (MQ=255)
                            aaaaCGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATgg                                                                                                             <  1:1353057/140‑1 (MQ=255)
                             aaaCGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGt                                                                                                            <  2:23760/140‑1 (MQ=255)
                             aaaCGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGt                                                                                                            <  2:3575774/140‑1 (MQ=255)
                             aaaCGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAAAATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGt                                                                                                            <  2:73333/140‑1 (MQ=255)
                                  gCATGTGAAAAAAAGAAGACTGCTAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTGCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACAt                                                                                                       <  2:4150317/140‑1 (MQ=255)
                                                gaagaCTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAgaga                                                                                         <  1:4137554/140‑1 (MQ=255)
                                                gaagaCTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAgaga                                                                                         <  1:1392072/140‑1 (MQ=255)
                                                gaagaCTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAgaga                                                                                         <  1:3376684/140‑1 (MQ=255)
                                                gaagaCTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAgaga                                                                                         <  1:3002928/140‑1 (MQ=255)
                                                gaagaCTGATAGGCGGAGCGGTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAgaga                                                                                         <  1:731549/140‑1 (MQ=255)
                                                           ggCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTAttt                                                                              >  2:2311100/1‑140 (MQ=255)
                                                             cGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACAttt                                                                                                     <  1:1011751/115‑1 (MQ=255)
                                                             cGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACAttt                                                                                                     >  2:1011751/1‑115 (MQ=255)
                                                              ggAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTAtttctt                                                                           >  2:2913615/1‑140 (MQ=255)
                                                               gAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTAtttcttt                                                                          >  2:1247467/1‑140 (MQ=255)
                                                                aGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCGGGTAAGCTGGCGTTTTCCGGTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTCtttctttc                                                                         >  2:489478/1‑140 (MQ=255)
                                                                aGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTTGCTTTAAGAGATTCCGCTAtttctttc                                                                         >  1:3381730/1‑140 (MQ=255)
                                                                aGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTAtttctttc                                                                         >  1:534182/1‑140 (MQ=255)
                                                                aGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTAtttctttc                                                                         >  1:1580145/1‑140 (MQ=255)
                                                                    ttCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCaaaa                                                                     >  2:3775524/1‑140 (MQ=255)
                                                                      cATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAAtt                                                                   >  2:3358100/1‑140 (MQ=255)
                                                                            ttCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTc                                                                                                                              <  2:2817300/75‑1 (MQ=255)
                                                                            ttCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTc                                                                                                                              >  1:2817300/1‑75 (MQ=255)
                                                                                      gTCCTTTTGATGTTTACTCCTTTTTCGCCGCTCGTGGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATGGTGATGGTGAGATGTGGCTTTAAGAGATTCCGCTATTTCTTTCACAATTTGTTTGACGTTTAtgt                                                   >  2:2474017/1‑138 (MQ=255)
                                                                                      gTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACCGTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCGTTTTAgtt                                                   >  1:2941688/1‑140 (MQ=255)
                                                                                      gTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACAtt                                                                                                      >  2:3343221/1‑89 (MQ=255)
                                                                                      gTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACAtt                                                                                                      <  1:3343221/89‑1 (MQ=255)
                                                                                      gTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACAt                                                                                                       <  1:1061093/88‑1 (MQ=255)
                                                                                      gTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACAt                                                                                                       >  2:1061093/1‑88 (MQ=255)
                                                                                      gTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTAttt                                                   >  2:809019/1‑140 (MQ=255)
                                                                                      gTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTAttt                                                   >  2:4246793/1‑140 (MQ=255)
                                                                                      gTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTAttt                                                   >  2:1445131/1‑140 (MQ=255)
                                                                                      gTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTAttt                                                   >  2:4230305/1‑140 (MQ=255)
                                                                                      gTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTAttt                                                   >  2:1123821/1‑140 (MQ=255)
                                                                                       tCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTAtttt                                                  >  1:3087722/1‑140 (MQ=255)
                                                                                         cTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGTTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGc                                                >  2:2083718/1‑140 (MQ=255)
                                                                                            ttGATGTTTACTCCTTTTTCGCCTATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCCGTTGTTATTGTGATGGTGACATTTGGCTGTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGAc                                             >  2:599808/1‑140 (MQ=255)
                                                                                            ttGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGAc                                             >  1:2348864/1‑140 (MQ=255)
                                                                                             ttaTGTTTACTCCATTTTCGGCTATCTTTGAACATCCGGCTTGTAAGCTGTCTTTTTCAGTTGTTATTGTTATGTTGACATTTGGCTTTAAGATATTCCGCTATTTATTTCAAAATTTGTTTTCCTTTTATTTTGCGaat                                            <  2:3528015/138‑3 (MQ=255)
                                                                                                 gTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACttttt                                        >  2:2649415/1‑140 (MQ=255)
                                                                                                    tACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAACATTTGTTTTCCTTTTATTTTGCGACTTTTTTaa                                     >  2:1479899/1‑140 (MQ=255)
                                                                                                    tACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTaa                                     <  2:3020363/140‑1 (MQ=255)
                                                                                                     aCTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAAt                                    <  1:1229468/140‑1 (MQ=255)
                                                                                                     aCTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAAt                                    <  1:911369/140‑1 (MQ=255)
                                                                                                     aCTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAAt                                    <  1:821717/140‑1 (MQ=255)
                                                                                                     aCTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAAt                                    <  1:4213527/140‑1 (MQ=255)
                                                                                                     aCTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAAt                                    <  1:3894891/140‑1 (MQ=255)
                                                                                                     aCTCCTTTTTCGCCGATCGTTGAACATCCGCCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAAt                                    <  1:1937255/140‑1 (MQ=255)
                                                                                                     aCTCCTATTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGTTGACATTTTGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTTTTTTGCGACTTTTTTAAt                                    <  1:1932817/140‑1 (MQ=255)
                                                                                                          tttttCGCCGATTGTTGAACATCCGGCTGGGAAGCTAGCTTTTTCAGTTGTGATTGTGGTGGTGACATTTGGCTTTAAAAGATTTCGGTTTTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCCACTTTTTTAATggggg                               <  2:3548819/140‑3 (MQ=255)
                                                                                                          tttttCGCCGATTGTTGAACATCCGGCTGGGAAGCTAGCTTTTTCAGTTGTGATTGTGGTGGTGACATTTGGCTTTAAAAGATTTCGGTTTTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCCACTTTTTTAATggggg                               <  2:3548947/140‑3 (MQ=255)
                                                                                                          tttttCGCCGATTGTTGAACATCCGGCTGGGAAGCTAGCTTTTTCAGTTGTGATTGTGGTGGTGACATTTGGCTTTAAAAGATTTCGGTTTTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCCACTTTTTTAATggggg                               <  2:3549298/140‑3 (MQ=255)
                                                                                                           ttttCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGggagg                              >  1:3027164/1‑140 (MQ=255)
                                                                                                           ttgtCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGggagg                              <  1:1872419/137‑1 (MQ=255)
                                                                                                            tttCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGggagga                             >  1:843158/1‑140 (MQ=255)
                                                                                                            tttCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGggagga                             >  1:1977536/1‑140 (MQ=255)
                                                                                                            tttCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTGTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGAGTTTTTTAATGggagga                             >  2:2131753/1‑140 (MQ=255)
                                                                                                            tttCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGAAATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGtgagga                             >  1:1708063/1‑140 (MQ=255)
                                                                                                                     tCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGGGAGGAGGAATTATc                    <  1:3087227/140‑1 (MQ=255)
                                                                                                                          gaccATCCGGCAGGTACGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGGTTTCCTTTTATTTTGCGACTTTTTTAATGGGAGGAGGAATTATCGGGGc               <  2:2075442/137‑1 (MQ=255)
                                                                                                                          gAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGGGAGGAGGAATTATCGGGGc               <  1:4051411/140‑1 (MQ=255)
                                                                                                                          gAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGGGAGGAGGAATTATCGGGGc               <  1:3750255/140‑1 (MQ=255)
                                                                                                                          gAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGGGAGGAGGAATTATCGGGGc               <  1:1329625/140‑1 (MQ=255)
                                                                                                                             cATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGGGAGGAGGAATTATCGGGGCCCa            <  2:2348864/140‑1 (MQ=255)
                                                                                                                             cATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGGGAGGAGGAATTATCGGGGCCCa            <  2:217502/140‑1 (MQ=255)
                                                                                                                             cATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGAGGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGGGAGGAGGAATTATCGGGGCCCa            <  2:4223407/140‑1 (MQ=255)
                                                                                                                             cATCCGGCAGGTAAGCTGGCATTTTCAGTGGGTATTGTGATCGTGACATTTGGCTTTAAGCCAGTCCGCTATTTCATTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGGGAGGAGGAATTATCGGGGCCCa            <  1:376228/140‑1 (MQ=255)
                                                                                                                                       gTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGGGAGGAGGAATTATCGGGGCCCATTCTTtgctg  <  1:1433188/140‑1 (MQ=255)
                                                                                                                                       gTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGGGAGGAGGAATTATCGGGGCCCATTCTTtgctg  <  1:1859453/140‑1 (MQ=255)
                                                                                                                                       gTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGGGAGGAGGAATTATCGGGGCCCATTCTTtgctg  <  1:2749895/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                          
TTTTGCTTTTGCTCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGGGAGGAGGAATTATCGGGGCCCATTCTTTGCTG  >  NZ_CP034943/1570368‑1570642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: