Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,570,510 G→A 5.2% L65L (CTG→CTA spoIIGA → sigma‑E processing peptidase SpoIIGA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,570,5100GA 5.2% 379.1 / 6.3 115L65L (CTG→CTAspoIIGAsigma‑E processing peptidase SpoIIGA
Reads supporting (aligned to +/- strand):  ref base G (47/62);  new base A (3/3);  total (50/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

CTTTTGCTCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGGGAGGAGGAATTATCGGGGCCCATTCTTTGCTGCAG  >  NZ_CP034943/1570373‑1570645
                                                                                                                                         |                                                                                                                                       
cttttgctCACGGCATTTATTTTAAAACGGCATGTGAAAAAAACAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGc                                                                                                                                       <  1:1545503/140‑1 (MQ=255)
   ttgctCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCttt                                                                                                                                    >  2:319489/1‑140 (MQ=255)
   ttgctCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCttt                                                                                                                                    <  2:1516020/140‑1 (MQ=255)
   ttgctCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCttt                                                                                                                                    <  2:1455888/140‑1 (MQ=255)
     gctCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCttttt                                                                                                                                  >  2:911369/1‑140 (MQ=255)
     gctCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCttttt                                                                                                                                  >  2:4213527/1‑140 (MQ=255)
     gctCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCttttt                                                                                                                                  >  2:3819488/1‑140 (MQ=255)
     gctCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCttttt                                                                                                                                  >  2:1229468/1‑140 (MQ=255)
     gctCACGGCATTTATTTTAAAACGGCATGTAAAAAAAAGAAGACTGGTAGGCGGAGCGTTCATCGGTTCCAGTATTGTCCTTTTGATGTTTACTCCTTTTTCGCCGATTGTTGAACATCCGGCTGGGAAGCTAGCttttt                                                                                                                                  >  1:3549298/1‑140 (MQ=255)
     gctCACGGCATTTATTTTAAAACGGCATGTAAAAAAAAGAAGACTGGTAGGCGGAGCGTTCATCGGTTCCAGTATTGTCCTTTTGATGTTTACTCCTTTTTCGCCGATTGTTGAACATCCGGCTGGGAAGCTAGCttttt                                                                                                                                  >  1:3548947/1‑140 (MQ=255)
     gctCACGGCATTTATTTTAAAACGGCATGTAAAAAAAAGAAGACTGGTAGGCGGAGCGTTCATCGGTTCCAGTATTGTCCTTTTGATGTTTACTCCTGTTTCGCCGATTGTTGAACATCCGGCTGGGAAGCTAGCttttt                                                                                                                                  >  1:3548819/1‑140 (MQ=255)
      ctCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTc                                                                                                                                 <  2:4102441/140‑1 (MQ=255)
      ctCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTc                                                                                                                                 <  2:3695995/140‑1 (MQ=255)
      ctCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTc                                                                                                                                 <  2:1580138/140‑1 (MQ=255)
      ctCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACCCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTc                                                                                                                                 <  2:3974831/140‑1 (MQ=255)
      ctCACGGCATTTATGTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTc                                                                                                                                 <  2:1085471/140‑1 (MQ=255)
        cACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAg                                                                                                                               <  1:2117524/140‑1 (MQ=255)
        cACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAg                                                                                                                               <  2:1346462/140‑1 (MQ=255)
         aCGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGATCCTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAgt                                                                                                                              <  2:4065437/140‑1 (MQ=255)
                  atttTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATtgt                                                                                                                     <  1:701079/140‑1 (MQ=255)
                     ttAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCATTTGTTATTGTGAt                                                                                                                  >  1:52669/1‑140 (MQ=255)
                     ttAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGAt                                                                                                                  >  1:3562016/1‑140 (MQ=255)
                     ttAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGAt                                                                                                                  >  1:3562098/1‑140 (MQ=255)
                       aaaaCGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATgg                                                                                                                <  1:1353057/140‑1 (MQ=255)
                        aaaCGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGt                                                                                                               <  2:2131050/140‑1 (MQ=255)
                        aaaCGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGt                                                                                                               <  2:3575774/140‑1 (MQ=255)
                        aaaCGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAAAATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGt                                                                                                               <  2:73333/140‑1 (MQ=255)
                             gCATGTGAAAAAAAGAAGACTGCTAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTGCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACAt                                                                                                          <  2:4150317/140‑1 (MQ=255)
                             gCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACAt                                                                                                          <  1:319489/140‑1 (MQ=255)
                                           gaagaCTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAgaga                                                                                            <  1:3002928/140‑1 (MQ=255)
                                           gaagaCTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAgaga                                                                                            <  1:3376684/140‑1 (MQ=255)
                                           gaagaCTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAgaga                                                                                            <  1:4137554/140‑1 (MQ=255)
                                           gaagaCTGATAGGCGGAGCGGTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAgaga                                                                                            <  1:731549/140‑1 (MQ=255)
                                                      ggCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTAttt                                                                                 >  2:2311100/1‑140 (MQ=255)
                                                        cGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACAttt                                                                                                        <  1:1011751/115‑1 (MQ=255)
                                                        cGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACAttt                                                                                                        >  2:1011751/1‑115 (MQ=255)
                                                         ggAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTAtttctt                                                                              >  2:2913615/1‑140 (MQ=255)
                                                          gAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTAtttcttt                                                                             >  2:1247467/1‑140 (MQ=255)
                                                           aGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCGGGTAAGCTGGCGTTTTCCGGTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTCtttctttc                                                                            >  2:489478/1‑140 (MQ=255)
                                                           aGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTTGCTTTAAGAGATTCCGCTAtttctttc                                                                            >  1:3381730/1‑140 (MQ=255)
                                                           aGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTAtttctttc                                                                            >  1:1580145/1‑140 (MQ=255)
                                                           aGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTAtttctttc                                                                            >  1:534182/1‑140 (MQ=255)
                                                           aGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAAAATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTAtttctttc                                                                            >  1:1362469/1‑140 (MQ=255)
                                                           aGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATAGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCAGCTAtttctttc                                                                            >  2:1076980/1‑140 (MQ=255)
                                                               ttCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCaaaa                                                                        >  2:3508481/1‑140 (MQ=255)
                                                               ttCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCaaaa                                                                        >  2:557812/1‑140 (MQ=255)
                                                               ttCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCaaaa                                                                        >  2:2976507/1‑140 (MQ=255)
                                                               ttCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCaaaa                                                                        >  2:3775524/1‑140 (MQ=255)
                                                                 cATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAAtt                                                                      >  2:3358100/1‑140 (MQ=255)
                                                                       ttCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTc                                                                                                                                 <  2:2817300/75‑1 (MQ=255)
                                                                                 gTCCTTTTGATGTTTACTCCTTTTTCGCCGCTCGTGGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATGGTGATGGTGAGATGTGGCTTTAAGAGATTCCGCTATTTCTTTCACAATTTGTTTGACGTTTAtgt                                                      >  2:2474017/1‑138 (MQ=255)
                                                                                 gTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACCGTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCGTTTTAgtt                                                      >  1:2941688/1‑140 (MQ=255)
                                                                                 gTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACAtt                                                                                                         >  2:3343221/1‑89 (MQ=255)
                                                                                 gTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACAt                                                                                                          <  1:1061093/88‑1 (MQ=255)
                                                                                 gTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACAt                                                                                                          >  2:1061093/1‑88 (MQ=255)
                                                                                 gTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTAttt                                                      >  2:1123821/1‑140 (MQ=255)
                                                                                 gTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTAttt                                                      >  2:1445131/1‑140 (MQ=255)
                                                                                 gTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTAttt                                                      >  2:809019/1‑140 (MQ=255)
                                                                                 gTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTAttt                                                      >  1:2919497/1‑140 (MQ=255)
                                                                                 gTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTAttt                                                      >  2:4230305/1‑140 (MQ=255)
                                                                                  tCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTAtttt                                                     >  1:3087722/1‑140 (MQ=255)
                                                                                    cTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGTTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGc                                                   >  2:2083718/1‑140 (MQ=255)
                                                                                       ttGATGTTTACTCCTTTTTCGCCTATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCCGTTGTTATTGTGATGGTGACATTTGGCTGTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGAc                                                >  2:599808/1‑140 (MQ=255)
                                                                                       ttGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGAc                                                >  1:2348864/1‑140 (MQ=255)
                                                                                        ttatttttACTGATTTTTCGCCTTTCGTTGAACATGCGGCATGTAAGCTGGCTTTTTCAGTTTTTATTTTGATGTTTAATTGTGGCTTTAAGCTATTCCGATATTTCTTTCTATTTTTGTTTTCATTTTTTTTTGCGata                                               <  2:2470577/135‑3 (MQ=255)
                                                                                        ttaTGTTTACTCCATTTTCGGCTATCTTTGAACATCCGGCTTGTAAGCTGTCTTTTTCAGTTGTTATTGTTATGTTGACATTTGGCTTTAAGATATTCCGCTATTTATTTCAAAATTTGTTTTCCTTTTATTTTGCGaat                                               <  2:3528015/138‑3 (MQ=255)
                                                                                        tGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTGTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACt                                               <  2:3573793/140‑1 (MQ=255)
                                                                                            gTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACttttt                                           >  2:2649415/1‑140 (MQ=255)
                                                                                               tACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAACATTTGTTTTCCTTTTATTTTGCGACTTTTTTaa                                        >  2:1479899/1‑140 (MQ=255)
                                                                                               tACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTaa                                        <  2:3020363/140‑1 (MQ=255)
                                                                                                aCTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAAt                                       <  1:1229468/140‑1 (MQ=255)
                                                                                                aCTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAAt                                       <  1:4213527/140‑1 (MQ=255)
                                                                                                aCTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAAt                                       <  1:821717/140‑1 (MQ=255)
                                                                                                aCTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAAt                                       <  1:3894891/140‑1 (MQ=255)
                                                                                                aCTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAAt                                       <  1:3819488/140‑1 (MQ=255)
                                                                                                aCTCCTTTTTCGCCGATCGTTGAACATCCGCCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAAt                                       <  1:1937255/140‑1 (MQ=255)
                                                                                                     tttttCGCCGATTGTTGAACATCCGGCTGGGAAGCTAGCTTTTTCAGTTGTGATTGTGGTGGTGACATTTGGCTTTAAAAGATTTCGGTTTTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCCACTTTTTTAATggggg                                  <  2:3548819/140‑3 (MQ=255)
                                                                                                     tttttCGCCGATTGTTGAACATCCGGCTGGGAAGCTAGCTTTTTCAGTTGTGATTGTGGTGGTGACATTTGGCTTTAAAAGATTTCGGTTTTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCCACTTTTTTAATggggg                                  <  2:3549298/140‑3 (MQ=255)
                                                                                                      ttttCGCCGATCGTTGAACATCCGGCATGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGggagg                                 <  1:1718953/140‑1 (MQ=255)
                                                                                                      ttttCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGggagg                                 <  1:833078/140‑1 (MQ=255)
                                                                                                      ttttCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGggagg                                 >  1:3027164/1‑140 (MQ=255)
                                                                                                      ttgtCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGggagg                                 <  1:1872419/137‑1 (MQ=255)
                                                                                                       tttCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGggagga                                >  1:2532011/1‑140 (MQ=255)
                                                                                                       tttCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGggagga                                >  1:1977536/1‑140 (MQ=255)
                                                                                                       tttCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGggagga                                >  1:843158/1‑140 (MQ=255)
                                                                                                       tttCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTGTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGAGTTTTTTAATGggagga                                >  2:2131753/1‑140 (MQ=255)
                                                                                                       tttCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGAAATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGtgagga                                >  1:1708063/1‑140 (MQ=255)
                                                                                                                tCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGGGAGGAGGAATTATc                       <  1:3087227/140‑1 (MQ=255)
                                                                                                                     gaccATCCGGCAGGTACGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGGTTTCCTTTTATTTTGCGACTTTTTTAATGGGAGGAGGAATTATCGGGGc                  <  2:2075442/137‑1 (MQ=255)
                                                                                                                     gAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGGGAGGAGGAATTATCGGGGc                  <  1:4051411/140‑1 (MQ=255)
                                                                                                                     gAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGGGAGGAGGAATTATCGGGGc                  <  1:1329625/140‑1 (MQ=255)
                                                                                                                     gAACATCCGGCAGGGAAGCTGGCTTTTTCAGTTGTTATTGTGATGGGGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGGGAGGAGGAATTATCGGGGc                  <  2:4171824/140‑1 (MQ=255)
                                                                                                                        cATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGGGAGGAGGAATTATCGGGGCCCa               <  2:2348864/140‑1 (MQ=255)
                                                                                                                        cATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGGGAGGAGGAATTATCGGGGCCCa               <  2:217502/140‑1 (MQ=255)
                                                                                                                        cATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGAGGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGGGAGGAGGAATTATCGGGGCCCa               <  2:4223407/140‑1 (MQ=255)
                                                                                                                        cATCCGGCAGGTAAGCTGGCATTTTCAGTGGGTATTGTGATCGTGACATTTGGCTTTAAGCCAGTCCGCTATTTCATTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGGGAGGAGGAATTATCGGGGCCCa               <  1:376228/140‑1 (MQ=255)
                                                                                                                                  gTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGGGAGGAGGAATTATCGGGGCCCATTCTTtgctg     <  1:1859453/140‑1 (MQ=255)
                                                                                                                                  gTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGGGAGGAGGAATTATCGGGGCCCATTCTTtgctg     <  1:1433188/140‑1 (MQ=255)
                                                                                                                                  gTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGGGAGGAGGAATTATCGGGGCCCATTCTTtgctg     <  1:2749895/140‑1 (MQ=255)
                                                                                                                                     aGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGGGAGGAGGAATTATCGGGGCCCATTCTTTGCTGCAg  <  1:1247467/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                       
CTTTTGCTCACGGCATTTATTTTAAAACGGCATGTGAAAAAAAGAAGACTGATAGGCGGAGCGTTCATCGGTTCCAGTATCGTCCTTTTGATGTTTACTCCTTTTTCGCCGATCGTTGAACATCCGGCAGGTAAGCTGGCTTTTTCAGTTGTTATTGTGATGGTGACATTTGGCTTTAAGAGATTCCGCTATTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCGACTTTTTTAATGGGAGGAGGAATTATCGGGGCCCATTCTTTGCTGCAG  >  NZ_CP034943/1570373‑1570645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: