BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,214,518	T→G	5.6%	K60T (AAA→ACA)	nth ←	endonuclease III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,214,518	0	T	G	5.6%	721.2 / 3.5	301	K60T (AAA→ACA)	nth	endonuclease III
Reads supporting (aligned to +/- strand): ref base T (49/221); new base G (22/9); total (71/230)
Fisher's exact test for biased strand distribution p-value = 3.42e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 306 total aligned reads displayed.

CTTCTCCGCCGTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGT‑ACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAT  >  NZ_CP034943/2214384‑2214657

cTTCTCCGCCGTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTatat                                                                                                                                         >  2:2890250/1‑140 (MQ=255)

cTTCTCCGCCGTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTatat                                                                                                                                         >  2:3824756/1‑140 (MQ=255)

cTTCTCCGCCGTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTatat                                                                                                                                         >  2:431903/1‑140 (MQ=255)

  tctcCGCCGTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATAttt                                                                                                                                       >  1:3398067/1‑140 (MQ=255)

     ccgccgTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTg                                                                                                                                    <  2:742270/140‑1 (MQ=255)

          gTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAaata                                                                                                                               <  2:3398067/140‑1 (MQ=255)

          gTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAaata                                                                                                                               <  1:1896888/140‑1 (MQ=255)

          gTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAaata                                                                                                                               <  2:500066/140‑1 (MQ=255)

           tAATCTTCGATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTTAaataa                                                                                                                              >  1:1001121/1‑140 (MQ=255)

             aTCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCCACTAGTCTTCCGGCCGTGTATTTTTTTGTAATAATg                                                                                                                            >  2:2314103/1‑140 (MQ=255)

             aTCTTCAATAATCATTTTGCTCAGGTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGGATATTGTTGAAATACTg                                                                                                                            >  1:957689/1‑140 (MQ=255)

              tCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGt                                                                                                                           <  2:38837/140‑1 (MQ=255)

              tCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGt                                                                                                                           <  2:1664942/140‑1 (MQ=255)

              tCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGt                                                                                                                           <  2:1851690/140‑1 (MQ=255)

                   aataatCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAAAAATGtttttg                                                                                                                      >  1:1162598/1‑138 (MQ=255)

                     taatCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTc                                                                                                                    <  2:2637013/140‑1 (MQ=255)

                        tCATTTTGCTCAGTTTTTTGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCAAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                 <  1:4008154/140‑1 (MQ=255)

                        tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                 <  1:2311119/140‑1 (MQ=255)

                        tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                 <  1:2680437/140‑1 (MQ=255)

                        tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                 <  1:3921750/140‑1 (MQ=255)

                        tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                 <  1:3172463/140‑1 (MQ=255)

                        tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                 <  1:3623770/140‑1 (MQ=255)

                        tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTGGAAATAATGTTTTGGTCAct                                                                                                                 <  1:82357/140‑1 (MQ=255)

                        acATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                 <  2:4288006/139‑1 (MQ=255)

                                   aGTTTTTGGATGTTTTTGGCTTTATTACTATACAAACCGATTTATTTAATATCGTGCTGAAGCTCTTCCAGAGAAACAGCCAAATAGTCTTCCGGCAGTTTATATTTTTGTAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                      <  2:2820647/140‑1 (MQ=255)

                                   aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTTCTATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                      <  2:781990/140‑1 (MQ=255)

                                   aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACATCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                      <  2:1224128/140‑1 (MQ=255)

                                   aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                      <  2:2965251/140‑1 (MQ=255)

                                   aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                      <  1:2188522/140‑1 (MQ=255)

                                   aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                      <  1:374063/140‑1 (MQ=255)

                                   aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                      <  2:1538065/140‑1 (MQ=255)

                                   aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                      <  1:3857748/140‑1 (MQ=255)

                                   aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                      <  1:313002/140‑1 (MQ=255)

                                   aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                      <  2:2711351/140‑1 (MQ=255)

                                   aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                      <  2:2213795/140‑1 (MQ=255)

                                   aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                      <  1:1755502/140‑1 (MQ=255)

                                   aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGAGTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                      <  2:3031612/140‑1 (MQ=255)

                                   aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACAGATTGATTTAATATCCTGCTGAAGCTCTACCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTGTGTTTACAAg                                                                                                      <  1:1552183/140‑1 (MQ=255)

                                   aGTTTTTGGATGTTTTTCGCTTTAGTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTGCCAGCGAAACAGCCAAATAGTCTTCCGGCCATTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                      <  2:3840091/140‑1 (MQ=255)

                                   aGTTTTTGGATGTTGTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                      <  2:4129905/140‑1 (MQ=255)

                                   aGTTTTTGGATGTCTTTCGCTTGATGTCGATACAAACCGATTGATTTAATATCCTGCTGAAGCACGTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGGTTCGATTTGTGAAATAATGTTTGGGTCACTCTGTTTACAAg                                                                                                      <  2:4062896/140‑1 (MQ=255)

                                     tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                    <  1:774819/140‑1 (MQ=255)

                                     tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                    <  2:2737758/140‑1 (MQ=255)

                                     tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                    <  1:2835291/140‑1 (MQ=255)

                                     tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                    <  2:1054582/140‑1 (MQ=255)

                                     tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                    <  1:3800329/140‑1 (MQ=255)

                                            aTGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAAGATCCTGCTGAAGCTCTTCCAGCGAAACAGCCACATAGTCTTCCGGCCGTTGATAGTGTTGGAATTATGGTGTGTTCCCTGTGTTTTCCAATGGATCTgg                                                                                             >  1:600368/1‑139 (MQ=255)

                                               tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTGACACTGTTTAAAAGTGCATATGT‑ACa                                                                                         >  1:1945954/1‑140 (MQ=255)

                                               tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTGTAATAATGTTTTTGTCACTCTGTTTAAAAGTTCATATGT‑aaa                                                                                         >  1:429280/1‑138 (MQ=255)

                                                  ttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTAGGTTTAAAAGTGCATCTGT‑ACATTg                                                                                      >  2:2407127/1‑140 (MQ=255)

                                                    cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGACACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGT‑ACATTgcg                                                                                    <  2:3742259/140‑1 (MQ=255)

                                                    cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGT‑ACATTgcg                                                                                    <  2:2512574/140‑1 (MQ=255)

                                                    cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGT‑ACATTgcg                                                                                    <  2:2163799/140‑1 (MQ=255)

                                                    cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGT‑ACATTgcg                                                                                    <  2:3443948/140‑1 (MQ=255)

                                                    cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGT‑ACATTgcg                                                                                    <  1:2893160/140‑1 (MQ=255)

                                                    cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGT‑ACATTgcg                                                                                    <  1:524333/140‑1 (MQ=255)

                                                    cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGT‑ACATTgcg                                                                                    <  2:3878796/140‑1 (MQ=255)

                                                    cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGT‑ACATTgcg                                                                                    <  1:3062742/140‑1 (MQ=255)

                                                    cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGT‑ACATTgcg                                                                                    <  2:410493/140‑1 (MQ=255)

                                                    cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGT‑ACATTgcg                                                                                    <  2:3357317/140‑1 (MQ=255)

                                                             aCGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGT‑ACATTGCGCAGATAAAg                                                                           <  2:1001121/140‑1 (MQ=255)

                                                                 tACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                    <  2:4022562/112‑1 (MQ=255)

                                                                   cAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGGATATTTTTTAAATAATGTTTTTGTCACTCTGTTTAAAAGTGAATCTGT‑ACAATTAGCAGATAAAGAGACTg                                                                     >  1:3491840/1‑140 (MQ=255)

                                                                     aaCCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGT‑ACATTGCGCAGATAAAGCGACTGCt                                                                   <  2:2670291/140‑1 (MQ=255)

                                                                         gattgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTTTATTTTTGAAATAATGTTTTTGTCACTCGGTTTACAAGTGCAACTGTAACATT‑CGCAGATAAAGCGACTGCTACCa                                                               >  1:632974/1‑140 (MQ=255)

                                                                          attgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGT‑ACATTGCGCAGATAAAGCGACTGCTACCAc                                                              <  2:117319/140‑1 (MQ=255)

                                                                           ttgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTTGTCACTCTGTTTACAAGTGCATATGT‑ACATTGCGCAGATAAAGCGACTGCTAACACt                                                             >  2:2759554/1‑140 (MQ=255)

                                                                           ttgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTAAATTTTTGAAATAATGTTTTGGTCACTCTGTTTTCAAGTGCATCTGT‑ACATTGCGGAGATAAAGAGACTGCTACCAca                                                             >  2:3192625/1‑139 (MQ=255)

                                                                                  aaTATCCTGCTGAAGATCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGT‑ACATTGCGCAGATAAAGCGACTGCTACCACTAATTCaa                                                      <  1:1308319/140‑1 (MQ=255)

                                                                                        ctgctgTAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGT‑ACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGAt                                                <  2:1467676/140‑1 (MQ=255)

                                                                                        ctgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTTTAATAATGTTTTGGTGACACTGTTTACAAGTGCATCTGT‑ACATTGCGCCGATAAAACGAATGCTACCACTAATTCAAAAGGAt                                                >  1:2373869/1‑140 (MQ=255)

                                                                                         tgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGT‑ACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGAtt                                               >  2:900473/1‑140 (MQ=255)

                                                                                         tgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTTAAATAATTTTTTTGTGACTCTGTTTACAATTTCATCTGT‑ACATTTCGCAGATAAATCCACTGCTACCAATAATTAAAAAGTAtt                                               >  1:4165092/1‑140 (MQ=255)

                                                                                          gctgAAGCTCTTCCAGCTAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGT‑ACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTg                                              <  1:3520431/140‑1 (MQ=255)

                                                                                                gCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTAAAAGTGCATCTGT‑ACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGa                                        >  2:3955957/1‑140 (MQ=255)

                                                                                                 ctctTCCAGCGAAACAGCCATATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGT‑ACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                       <  2:722254/140‑1 (MQ=255)

                                                                                                 ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGT‑ACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                       <  1:900473/140‑1 (MQ=255)

                                                                                                 ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGT‑ACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                       <  1:4335868/140‑1 (MQ=255)

                                                                                                 ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGT‑ACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                       <  2:3087624/140‑1 (MQ=255)

                                                                                                 ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGT‑ACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAACGaa                                       <  1:3262899/140‑1 (MQ=255)

                                                                                                 ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGAATCTGT‑ACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                       <  2:3654833/140‑1 (MQ=255)

                                                                                                  tctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGT‑ACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAAc                                      <  1:1612813/140‑1 (MQ=255)

                                                                                                   ctTCCAGCGAAACAGCCAAATATTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGT‑ACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAAcc                                     <  2:179510/140‑1 (MQ=255)

                                                                                                   ctTCCAGCGAAACAGCCAAATAGTATTCCGGCCTTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGT‑ACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAAcc                                     <  2:3312819/140‑1 (MQ=255)

                                                                                                   cgtCCAGCGAAACAGCCAAATAGTGTTCGGGCCGTTTCTCTTTTTGTAATACTGTTTTGGTCACTCTGTTTACAAGTGCATCTGT‑ACATTGCGGAGATAAAGCGACTGCTACCACTACTTCAAAAGGATTGGAATGAAcc                                     <  2:1960358/138‑1 (MQ=255)

                                                                                                   cgtCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTCAAATAATGTTTTGGTCAATCTGTTTACAAGTGCATCTGT‑ACATTTCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAAcc                                     <  1:1801779/138‑1 (MQ=255)

                                                                                                     tCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTTTAAAAATTTTTTTGTGACTCTGTTTTAAAGTGCATCTGT‑ACAATGCGCAGCTTAATCGACTGCTACCACTAATTGAAAAGGAGTGGGATGAACCAg                                   >  1:3359843/1‑140 (MQ=255)

                                                                                                       cAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGT‑ACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGtt                                 <  1:1354236/140‑1 (MQ=255)

                                                                                                       cAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGT‑ACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGtt                                 <  1:2484480/140‑1 (MQ=255)

                                                                                                             aaCAGCCAAATAGTCTTCCGGCCTTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACACGTGCATCTGT‑ACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACAct                           <  1:209283/140‑1 (MQ=255)

                                                                                                                aGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGT‑ACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTg                        <  2:16276/140‑1 (MQ=255)

                                                                                                                 gCCAAATAGTCTTCCGGCCGTGTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGT‑ACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGc                       <  2:31560/140‑1 (MQ=255)

                                                                                                                       tAGTCTTCCGGCCGTTTATATTTTTTAAAAAAAGTTTTGGTCACTCTGTTTACAAGTGCATCTGT‑AAAATTCGCAGATAAAGCGACTGCTACCACGAATTCAAAAAGATTGGAATGAAACACTTAACCCACTGCGGGTgg                 >  2:96110/1‑140 (MQ=255)

                                                                                                                        aGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGT‑ACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGa                <  2:2035968/140‑1 (MQ=255)

                                                                                                                            ttCCGGCCGTTTATATTTTTTAAATAATGTTTTTGTCACTCTGTTTAAAAGTGCATCTGT‑ACATTGCGCAGATAAAGCGACTGCTAACACTAAATCAAAAGGATTGGAATGAAACAGTTCACACACTGCGTGTGGAAACa            >  2:1818912/1‑140 (MQ=255)

                                                                                                                            ttCCGGCCGTTTATATTTTTGCAAAAATGTTTTGGTCCCTCGGGTTAAAAGTGCATCTTT‑ACATTGCGCCGATAAACCGACTTCTACCACAAAGTAAAAAGGATTGGAATTCACCAGGTAACACTCTGCCTGTGGaaaac            >  2:554669/1‑138 (MQ=255)

                                                                                                                               cGGCCGTTTATATTTTTGAAAAAATGTTTTTGTCACTCAGTTTACAAGTGCATCTGT‑ACAATGCGCAGATAAATCGACTGCTCCCACAAAATCAAAAGGATTGGAAAGAACCAGTTCACAATCAGCGTGTGGAAACaatt         >  2:2055965/1‑137 (MQ=255)

                                                                                                                                    gTTTATATTTTTTAAATAATTTTTTGGTCACTCTGTTTAAAAGTGCATCTGT‑ACATTGCGCAGATAAAGCGACTGCTACAACTAAATAAAAAGGATTTGAATTAAACACTTAAAACAATGCGTGTGGAAACATGTCGcca    >  2:1360218/1‑139 (MQ=255)

                                                                                                                                      ttATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGT‑ACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt  <  2:421217/140‑1 (MQ=255)

                                                                                                                                      ttATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGT‑ACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt  <  1:1833692/140‑1 (MQ=255)

CTTCTCCGCCGTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGT‑ACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAT  >  NZ_CP034943/2214384‑2214657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`