Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,300,863 T→G 7.0% intergenic (‑194/+202) ribD ← / ← EO946_RS11710 bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5‑amino‑6‑(5‑phosphoribosylamino)uracil reductase RibD/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,300,8630TG 7.0% 600.5 / 8.9 254intergenic (‑194/+202)ribD/EO946_RS11710bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5‑amino‑6‑(5‑phosphoribosylamino)uracil reductase RibD/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (116/111);  new base G (3/23);  total (119/135)
Fisher's exact test for biased strand distribution p-value = 1.06e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 260 total aligned reads displayed.

AAAATCCGGGGCTTTAGGTATTTTACGCAATAGGAAATAACACTATGCATTTTTGATACAATTGCATAGCGGCACATCATCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTCATTTGTCCTTATTGGCTATTATAGCGATCCTTTTTAAAGTACGCA  >  NZ_CP034943/2300725‑2300999
                                                                                                                                          |                                                                                                                                        
 aaaTCCGGGGCTTTAGGTATTTTACGCAATAGGAAATAACACTATGCATTTTTGATACAATTGCATAGCGGCAAATCATCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTa                                                                                                                                        >  2:4274785/1‑140 (MQ=255)
  aaTCCGGGGCTTTAGGTATTTTACGCAATAGGAAATAACACTATGCATTTTTGATACAATTGCATAGCGGCACATCATCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTAt                                                                                                                                       >  2:1395576/1‑140 (MQ=255)
       ggggCTTTAGGTATTTTACGCAATAGGAAATAACACTATGCATTTTTGATACAATTGCATAGCGGCACATCATCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGcaca                                                                                                                                  <  1:928976/140‑1 (MQ=255)
       ggggCTTTAGGTATTTTACGCAATAGGAAATAACACTATGCATTTTTGATACAATTGCATAGCGGCACATCATCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGcaca                                                                                                                                  <  1:79799/140‑1 (MQ=255)
       ggggCTTTAGGTATTTTACGCAATAGGAAATAACACTATGCATTTTTGATACAATTGCATAGCGGCACATCATCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGcaca                                                                                                                                  <  2:3871175/140‑1 (MQ=255)
       ggggCTTTAGGTATTTTACGCAATAGGAAATAACACTATGCATTTTTGATACAATTGCATAGCGGCACATCATCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTAGGcaca                                                                                                                                  <  2:2806411/140‑1 (MQ=255)
       ggggCTTTAGGTATTTTACGCAATAGGAAATAACACTATGCATTTTTGATACAATTGCATAGCGGCACATCATCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTGATGcaca                                                                                                                                  <  2:3633044/140‑1 (MQ=255)
       ggggCTTTAGGTATTTTACGCAATAGGAAATAACACTATGCATTTTTGATACAATTGCATAGCGGCACATCATCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCGTATGcaca                                                                                                                                  <  1:3838533/140‑1 (MQ=255)
       ggggCTTTAGGTATTTTACGCAATAGGAAATAACACTATGCATTTGTGATACAATTGCATAGCGGCACATCATCCGTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGcaca                                                                                                                                  <  2:1220622/140‑1 (MQ=255)
               aGGTATTTTACGCAATAGGAAATAACACTATGCATTTTTGATACAATTGCATAGCGGCACATCATCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCAc                                                                                                                          <  1:747885/140‑1 (MQ=255)
               aGGTATTTTACGCAATAGGAAATAACACTATGCATTTTTGATACAATTGCATAGCGGCACATCATCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCAc                                                                                                                          <  1:178160/140‑1 (MQ=255)
                ggTATTTTACGCAATAGGAAATAACACTATGCATTTTTGATACAATTGCATAGCGGCACATCATCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACg                                                                                                                         <  1:2040939/140‑1 (MQ=255)
                ggTATTTTACGCAATAGGAAATAACACTATGCATTTTTGATACAAGTGCATAGCGGCACCTCATCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTAGGCACACGGGTCACg                                                                                                                         <  1:2538433/140‑1 (MQ=255)
                              tAGGAAATAACACTATGCATTTTTGATACAATTGCATAGCGGCACATCATCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGAGCGCTTATACACACGGGTCACGGGCTCTAAAGCGaa                                                                                                           >  1:3981143/1‑140 (MQ=255)
                                      aaCACTATGCATTTTTGATACAATTGCATAGCGGCACATCATCCTTCTCCCATCCCGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACCCGGGTCACGGGCTCTAAAGCGAATTCGCttt                                                                                                   >  1:3223550/1‑140 (MQ=255)
                                      aaCACTATGCATTTTTGATACAATTGCATAGCGGCACATCATCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCttt                                                                                                   >  1:2190595/1‑140 (MQ=255)
                                        cacTATGCATTTTTGATACAATTGCATAGCGGCACATCATCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTtac                                                                                                 >  1:331687/1‑140 (MQ=255)
                                        caatATGCATTTTTGATACAATAGCATAGCGGCACATCATCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTGAAACTGAATCCACCGAGCGCTGATGCACACGGGTCACGTGATCTAAAGAGAATTCGCTTtac                                                                                                 >  1:3477347/4‑140 (MQ=255)
                                          cTATGCATTTTTGATACAATTGCATAGCGGCACATCATCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTtacta                                                                                               >  1:953410/1‑140 (MQ=255)
                                           tATGCATTTTTGATACAATTGCATCGCGGCACATCATCCTTGTACGATCCAGACTTTGACTGTCGGCTTGAGCTTAAACTGAATCAACCGCGCGATGATGCACACGGGGCACGGGCTCTAAAGCGAATTCGCTGtacgcc                                                                                              >  2:2644830/1‑137 (MQ=255)
                                           tATGCATTTTTGATACAATTGCATAGCGGCACATCATCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTtactac                                                                                              >  2:492006/1‑140 (MQ=255)
                                             tGCATTTTTGATACAATTGCATAGCGGCACATCATCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTAccg                                                                                            >  1:447674/1‑140 (MQ=255)
                                             tGCATTTTTGATACAATTGCATAGCGGCACATCATCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTAccg                                                                                            >  1:1692058/1‑140 (MQ=255)
                                             tGCATTTTTGATACAATTGCATAGCGGCACATCATCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTAccg                                                                                            >  1:3765803/1‑140 (MQ=255)
                                             tGCATTTTTGATACAATTGCATAGCGGCACATCATCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTAccg                                                                                            >  2:2122569/1‑140 (MQ=255)
                                             tGCATTTTTGATACAATTGCATAGCGGCACATCATCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTAccg                                                                                            <  1:3362729/140‑1 (MQ=255)
                                             tGCATTTTTGATACAATTGCATAGCGGCACATCATCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTAccg                                                                                            >  2:2918785/1‑140 (MQ=255)
                                             tGCATTTTTGATACAATTGCATAGCGGCACATCATCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTAccg                                                                                            >  2:2943135/1‑140 (MQ=255)
                                             tGCATTGTTGATACAATTGCATAGCGGCACATCCTCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAACCTGAATCCACCGGGCGCTGAGGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTAccg                                                                                            <  1:3359390/140‑1 (MQ=255)
                                               cATTTTTGATACAATTGCATAGCGGCACATCATCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTAccgcc                                                                                          >  2:342867/1‑140 (MQ=255)
                                                   tttGATACAATTGCATAGCGGCACATCATCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTc                                                                                      >  1:1247773/1‑140 (MQ=255)
                                                   tttGATACAATTGCATAGCGGCACATCATCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTc                                                                                      <  1:1983892/140‑1 (MQ=255)
                                                   tttGATACAATTGCATAGCGGCACATCATCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTc                                                                                      <  1:1835526/140‑1 (MQ=255)
                                                   tttGATACAATTGCATAGCGGCACATCATCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTc                                                                                      >  1:170206/1‑140 (MQ=255)
                                                     tGATACAATTGCATAGCGGCACATCATCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCgg                                                                                    <  1:3326295/140‑1 (MQ=255)
                                                     tGAGACAATTGCATAGCGGCACATCATCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCgg                                                                                    <  1:1278943/140‑1 (MQ=255)
                                                               gCATAGCGGCACATCAGCCTTCGCCCCTCCAGACTGTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCGTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCAcc                                                                          <  2:3344613/140‑1 (MQ=255)
                                                                    gCGGCACATCATCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGcc                                                                     <  2:331687/140‑1 (MQ=255)
                                                                     cGGCACATCATCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGccc                                                                    >  1:3890395/1‑140 (MQ=255)
                                                                     cGGCACATCATCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCGGccc                                                                    >  1:2840170/1‑140 (MQ=255)
                                                                      ggCACATCATCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGcccc                                                                   >  1:3680289/1‑140 (MQ=255)
                                                                        cacaTCATCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGa                                                                 <  2:752958/140‑1 (MQ=255)
                                                                             catcCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGTGATTTCCACCCTGCCCCGAAGGAt                                                            >  1:3083814/1‑140 (MQ=255)
                                                                              atcCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATa                                                           <  2:177360/140‑1 (MQ=255)
                                                                              atcCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATa                                                           <  1:3927179/140‑1 (MQ=255)
                                                                              atcCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCGTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATa                                                           <  1:2368067/140‑1 (MQ=255)
                                                                               tcCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTGATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATAc                                                          <  1:2976826/140‑1 (MQ=255)
                                                                                  ttCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAAt                                                       >  1:2104376/1‑140 (MQ=255)
                                                                                     tcCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGa                                                                 >  1:1941174/1‑127 (MQ=255)
                                                                                       ccATCCAGACTTTCACTGTCGGCTTCAGGTTAAACTGAATCCACCGCGCGCGTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTGTACTACCGCCGGTCGGGATGTCCACCCTGCCCCGACGGATACAATCTGTa                                                  >  1:3396766/1‑140 (MQ=255)
                                                                                         aTCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTAtt                                                >  2:3480454/1‑140 (MQ=255)
                                                                                          tCCAGCCTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCAGGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGAGACAATCTTTATTc                                               >  2:412010/1‑140 (MQ=255)
                                                                                          tCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTc                                               >  2:1662775/1‑140 (MQ=255)
                                                                                          tCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTc                                               >  1:116423/1‑140 (MQ=255)
                                                                                          tCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTc                                               >  1:3277443/1‑140 (MQ=255)
                                                                                          tCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTc                                               >  1:3788246/1‑140 (MQ=255)
                                                                                          tCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCAACCCTGCCCCGAAGGATACAATATTTATTc                                               >  1:3641780/1‑140 (MQ=255)
                                                                                             aGACTTTCAGTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTCTGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACCATCTTTATTCAtt                                            <  2:3680420/140‑1 (MQ=255)
                                                                                             aGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTTTGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGTTTTCCACCCTGCCCCGAAGGATACAATCTTTATTCAtt                                            <  2:3184580/140‑1 (MQ=255)
                                                                                                 tttCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCAc                                                                                                                          <  2:1160859/58‑1 (MQ=255)
                                                                                                 tttCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTCATTTGTc                                        >  1:1160047/1‑140 (MQ=255)
                                                                                                 tttCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTCATTTGTc                                        >  1:2930266/1‑140 (MQ=255)
                                                                                                    cACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTCATTTGTCCtt                                     >  2:1579018/1‑140 (MQ=255)
                                                                                                     aCTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTCATTTGTCCTTa                                    <  1:882181/140‑1 (MQ=255)
                                                                                                     aCTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTCATTTGTCCTTa                                    <  2:447674/140‑1 (MQ=255)
                                                                                                       tGTCGGCTTCCGCTTAAACTGAATCCACCGCGCGCTTCTGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTCATTTGTCCTTAtt                                  <  2:884007/140‑1 (MQ=255)
                                                                                                       tGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTCATTTGTCCTTAtt                                  <  2:1221007/140‑1 (MQ=255)
                                                                                                       tGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTCATTTGTCCTTAtt                                  <  2:2431701/140‑1 (MQ=255)
                                                                                                       tGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTCATTTGTCCTTAtt                                  <  2:3742338/140‑1 (MQ=255)
                                                                                                       tGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTCATTTGTCCTTAtt                                  <  1:342867/140‑1 (MQ=255)
                                                                                                       tGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTCATTTGTCCTTAtt                                  <  2:3290161/140‑1 (MQ=255)
                                                                                                         tCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTCATTTGTCCTTATTgg                                <  1:1541026/140‑1 (MQ=255)
                                                                                                         tCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTCATTTGTCCTTATTgg                                >  2:2594504/1‑140 (MQ=255)
                                                                                                         tCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTAGGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTCATTTGTCCTTATTgg                                <  1:3260251/140‑1 (MQ=255)
                                                                                                           ggCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTCATTTGTCCTTATTGGCt                              >  2:3057537/1‑140 (MQ=255)
                                                                                                             cTTCAGCTTAAACTGAGTCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTCATTTGTCCTTATTGGCtat                            <  1:580086/140‑1 (MQ=255)
                                                                                                             cTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTCATTTGTCCTTATTGGCtat                            <  1:2263876/140‑1 (MQ=255)
                                                                                                             cTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTCATTTGTCCTTATTGGCtat                            <  1:4158704/140‑1 (MQ=255)
                                                                                                                 aGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTCATTTGTCCTTATTGGCTATtata                        >  2:1657116/1‑140 (MQ=255)
                                                                                                                   cTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGcc                                                                     <  2:3822563/93‑1 (MQ=255)
                                                                                                                    ttAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTCATTTGTCCTTATTGGCTATTATAGCg                     <  2:3988881/140‑1 (MQ=255)
                                                                                                                      taaCTGAATGCAGAGCGCGAGTATGCACACGGGTCACGGGCTATAAAGCGAATTGGCTTTACGACCGCCGGTCGGGATTTCCAGCCTGCGCGGAAGGATACACTCTTTATTCATTTGTCCTTATTGGCTATTATAGCGAt                   <  2:3602795/139‑1 (MQ=255)
                                                                                                                      ataCTGAATCCACCGCGCGCCTATGCACACGGGTCACGGGCTCTAACGCGAATTCGCTTTACTACCGCCGGTCGGGAATTCCACCCGGCCCCGACGGATACACTCTTTCTAGATTTGTCCTTATTGGCTATTATAGCgct                   <  2:1848525/138‑3 (MQ=255)
                                                                                                                      aaaCTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTCATTTGTCCTTATTGGCTATTATAGCGAt                   <  2:2070706/140‑1 (MQ=255)
                                                                                                                      aaaCTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTCATTTGTCCTTATTGGCTATTATAGCGAt                   <  1:2892275/140‑1 (MQ=255)
                                                                                                                      aaaCTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTCATTTGTCCTTATTGGCTATTATAGCGAt                   <  2:2277652/140‑1 (MQ=255)
                                                                                                                      aaaCTGAATCCACCGCGCGCTTAGGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTCATTTGTCCTTATTGGCTATTATAGCGAt                   <  1:3999780/140‑1 (MQ=255)
                                                                                                                      aaaCTGAATCCACCGCGCGCGTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTCATTTGTCCTTATTGGCTATTATAGCGAt                   <  1:2372313/140‑1 (MQ=255)
                                                                                                                      aaaCTGAATCCACCGCGCGCGGATGCACACGGGTCCCGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCGGCCCCGAACGATACAAACTTTATTCATTTGTCCATATTGGCTATTATAGCGAt                   <  2:3976473/140‑1 (MQ=255)
                                                                                                                            aaTCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTTCACCCTGCCCCGAAGGATACAATCTTTATTCATTTGTCCTTATTGGCTATTATAGCGATCCtttt             >  2:642887/1‑140 (MQ=255)
                                                                                                                            aaTCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTCATTTGTCCTTATTGGCTATTATAGCGATCCtttt             >  2:4213318/1‑140 (MQ=255)
                                                                                                                            aaTCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTCATTTGTCCTTATTGGCTATTATAGCGATCCtttt             >  2:1937409/1‑140 (MQ=255)
                                                                                                                            aaTCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTCATTTGTCCTTATTGGCTATTATAGCGATCCtttt             >  1:882694/1‑140 (MQ=255)
                                                                                                                            aaTCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTCATTTGTCCTGATTGGCTATTATAGCGATCCttgt             >  2:233907/1‑138 (MQ=255)
                                                                                                                             aTCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATAc                                                          >  1:1386386/1‑94 (MQ=255)
                                                                                                                               ccACCGCGCGCTGAGGCACACGGGTCACGCGCTCGAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTCATTTGTCCTTATTGGCTATTATAGCGATCCTTTTTaa          <  2:1638100/140‑1 (MQ=255)
                                                                                                                                   cgcgcgCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTCATTTGTCCTTATTGGCTATTATAGCGATCCTTTTTAAAGTa      <  1:3613310/140‑1 (MQ=255)
                                                                                                                                       cgcTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTCATTTGTCCTTATTGGCTATTATAGCGATCCTTTTTAAAGTACGCa  <  1:2918785/140‑1 (MQ=255)
                                                                                                                                       cgcTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTCATTTGTCCTTATTGGCTATTATAGCGATCCTTTTTAAAGTACGCa  <  1:3015529/140‑1 (MQ=255)
                                                                                                                                       cgcTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTCATTTGTCCTTATTGGCTATTATAGCGATCCTTTTTAAAGTACGCa  <  1:1569755/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                        
AAAATCCGGGGCTTTAGGTATTTTACGCAATAGGAAATAACACTATGCATTTTTGATACAATTGCATAGCGGCACATCATCCTTCTCCCATCCAGACTTTCACTGTCGGCTTCAGCTTAAACTGAATCCACCGCGCGCTTATGCACACGGGTCACGGGCTCTAAAGCGAATTCGCTTTACTACCGCCGGTCGGGATTTCCACCCTGCCCCGAAGGATACAATCTTTATTCATTTGTCCTTATTGGCTATTATAGCGATCCTTTTTAAAGTACGCA  >  NZ_CP034943/2300725‑2300999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: