BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,350,847	G→T	7.9%	pseudogene (934/1182 nt)	EO946_RS12020 ←	IS3 family transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,350,847	0	G	T	7.9%	643.5 / 3.8	255	pseudogene (934/1182 nt)	EO946_RS12020	IS3 family transposase
Reads supporting (aligned to +/- strand): ref base G (53/169); new base T (29/4); total (82/173)
Fisher's exact test for biased strand distribution p-value = 1.37e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 256 total aligned reads displayed.

CTTTTTCGGTTTGACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGAC  >  NZ_CP034943/2350709‑2350982

  ttttCGGTTTGACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGata                                                                                                                                       <  1:3770894/140‑1 (MQ=255)

  ttttCGGTTTGACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGata                                                                                                                                       <  2:1701733/140‑1 (MQ=255)

  ttttCGGTTTGACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGata                                                                                                                                       <  1:982727/140‑1 (MQ=255)

  ttttCGGTTTGACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGata                                                                                                                                       <  2:3688550/140‑1 (MQ=255)

       ggTTTGACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGTCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCtt                                                                                                                                  <  1:910683/140‑1 (MQ=255)

         tttGACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTGTTGATATTGtttt                                                                                                                                >  1:2213632/1‑140 (MQ=255)

           tGACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTTATATGGTTttgt                                                                                                                              >  2:598767/1‑140 (MQ=255)

           tGACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTTGTTTTGCTTttgg                                                                                                                              >  1:3046974/1‑139 (MQ=255)

            gACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAATAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTttgtt                                                                                                                             <  1:2960173/140‑1 (MQ=255)

            gACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAACACCATTCGATACAGGCATTATCGAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTttgtt                                                                                                                             <  2:545363/140‑1 (MQ=255)

            gACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTttgtt                                                                                                                             <  2:1022400/140‑1 (MQ=255)

            gACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTttgtt                                                                                                                             <  2:1264104/140‑1 (MQ=255)

            gACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTttgtt                                                                                                                             <  1:509010/140‑1 (MQ=255)

                   tGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTTTGTTTTCTTTTTTTTTGTAtt                                                                                                                      >  1:596747/1‑140 (MQ=255)

                   tGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTTGATATTGGTTTGTTGTGTAtt                                                                                                                      >  1:1176643/1‑140 (MQ=255)

                    gAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTg                                                                                                                     <  1:1856954/140‑1 (MQ=255)

                     aaaaaTATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTgg                                                                                                                    <  1:1851045/140‑1 (MQ=255)

                     aaaaaTATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTgg                                                                                                                    <  1:2176454/140‑1 (MQ=255)

                          tatTCATTCTCTGTTTTTAGATGCGTGTAAAACCATTCGATACAGTCATTATCTAGGCAGTTTGGCTTACGGGAATGGCTGCCTTTCTATCCTACTGTTTCTATTTGTTTTTTTTATGCTTTTGTTGTGTAtttgtagac                                                                                                               <  2:3043925/140‑8 (MQ=255)

                          tatGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGcc                                                                                                               <  2:712118/140‑1 (MQ=255)

                          tatGCATTCTCTGGTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGcc                                                                                                               <  2:3070778/140‑1 (MQ=255)

                            tGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCtt                                                                                                             <  2:3539604/140‑1 (MQ=255)

                            tGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTTGAGATGGGTTTGTTGTGTTTTGGAAGccgt                                                                                                             >  2:3609618/1‑138 (MQ=255)

                               aTTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACATGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGt                                                                                                          <  2:965816/140‑1 (MQ=255)

                               aTTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGt                                                                                                          <  1:696557/140‑1 (MQ=255)

                               aTTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACAAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGt                                                                                                          <  2:2064413/140‑1 (MQ=255)

                               aTTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACAAACGGTTTCGAGTCGCGTTTGATAATCGTTTTTTGTGTGTTGGTAGCCTTgtt                                                                                                          >  2:2607142/1‑138 (MQ=255)

                               aTTCTCTGTTTTGACAGGCTAGAACAACCATGCGATACCGGCCTGATCGAGGCAGTGTGCCTTACGGGAATGGCTGCCTTTCAACCCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGt                                                                                                          <  2:2692712/140‑1 (MQ=255)

                                 tctcTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCa                                                                                                        <  1:4196013/140‑1 (MQ=255)

                                 tctcTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCa                                                                                                        <  1:808549/140‑1 (MQ=255)

                                 tctcTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCa                                                                                                        <  1:1511082/140‑1 (MQ=255)

                                 tctcTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCa                                                                                                        <  1:3974394/140‑1 (MQ=255)

                                   tctGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTTTTTATGCGTTTTTTTTTTATTTGAAGCCTTTGTCAGa                                                                                                      >  1:1010981/1‑140 (MQ=255)

                                   tctGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTGTTATATGGTTTTGTTGTGTATTGGAAGCCGTGGTgaga                                                                                                      >  1:2915566/1‑140 (MQ=255)

                                    ctGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTGTTTTGTTGGTTTGTTTTGTATTTGAAGCCTATGTCAGaa                                                                                                     >  1:3630292/1‑140 (MQ=255)

                                    ctGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTGTTATATTGTTTTTTTGTGGATTTGTAGCCTTTGTGAGaa                                                                                                     >  1:2530545/1‑140 (MQ=255)

                                    ctGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGGTTGTTATGGGTGTGTTTTGTGTTTGTAGGCCTGGTGAGaa                                                                                                     >  1:3688945/1‑140 (MQ=255)

                                     tGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATTCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAAt                                                                                                    >  2:2382571/1‑140 (MQ=255)

                                     tGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTTGata                                                                                                                                       >  2:2185349/1‑105 (MQ=255)

                                             gaTGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATa                                                                                            <  2:2827237/140‑1 (MQ=255)

                                               tGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTTTATATGGTTTTGTTTTTTATTTGAAGCCATTGGCACAATGGCGGGTAga                                                                                          >  1:1056565/1‑139 (MQ=255)

                                                 cGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTTTTATGGTTTTTTTGTGTATTTGAAAACGTGGTGAGAATGgatgatttctc                                                                                        >  2:1805881/1‑135 (MQ=255)

                                                 cGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGGTATGCTTTTTGTTTGGAATTGAACCCGTGGTCACGAAGGAGCGTAGCTc                                                                                        >  1:1637399/1‑140 (MQ=255)

                                                 cGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGGGTTTGTTTTGTATTTGAATCCTTTGTCAGAATGGAGGATAGCTc                                                                                        >  2:4247272/1‑140 (MQ=255)

                                                 cGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTTGTTATGCTTGTGTTGTGTATTGGTAGCCTTGGTCAGAATGGAGGATAGCTc                                                                                        >  2:4290331/1‑140 (MQ=255)

                                                 cGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTTGATATTGTTTTGTTTTTTATTTGAATCCGTTGTGAGAATGGAGGATAGGTc                                                                                        >  1:1951912/1‑140 (MQ=255)

                                                 cGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGGTTCGAGTCGCTTTTGGTATGCTTTTGTTTTGGCTTGGTCCCGGTGGTGGGAATGGAGGATatagc                                                                                        >  2:2472784/1‑136 (MQ=255)

                                                         aCCATTGGATACAGTCATTATCTAGGCAGTTTGCCTTACGCGAATGGCTGCCTTTCAAACCAATGGTTTCGAGTCTCTTTTGATATGCTTTTGTTGTGTATTGGATGCGTTGGTCAGAATGGATGATATCTCATTGGACg                                                                                <  2:1178513/140‑1 (MQ=255)

                                                         aCCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAAGTGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACg                                                                                <  1:238667/140‑1 (MQ=255)

                                                         aCCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCCTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACg                                                                                <  2:884407/140‑1 (MQ=255)

                                                                 aTACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGGTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTg                                                                       <  1:72951/140‑1 (MQ=255)

                                                                   aCAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTggg                                                                     <  1:4322704/140‑1 (MQ=255)

                                                                   aCAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTggg                                                                     <  1:2141672/140‑1 (MQ=255)

                                                                   aCAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTggg                                                                     <  2:2918857/140‑1 (MQ=255)

                                                                   aCAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTggg                                                                     <  2:948183/140‑1 (MQ=255)

                                                                    cAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGATTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGc                                                                    <  1:689117/140‑1 (MQ=255)

                                                                        cATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTGGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTc                                                                <  2:2280746/140‑1 (MQ=255)

                                                                          ttATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATTTTCTTTTTTTGTGTATTTGTAACCGTGGTGCGTATTGAGGATAGCTCCGTGGAAGTGCCGGTTggggttaat                                                               >  2:689117/1‑134 (MQ=255)

                                                                           tATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGTTATGGTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATTGCGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATa                                                             >  2:3922952/1‑140 (MQ=255)

                                                                           tATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTTGATATGGTTTTGTTTTGTATTTGAAGCCGTTGTGAGAAAGGAGGTTAGCTCCTTGGACGT‑GCCGTTGGGCTGTCaaa                                                             >  2:1418932/1‑138 (MQ=255)

                                                                           tATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGGTTTTTAATCGTTTGTTGTGTTTTGGAAGCCTTGGTCAGAATGGGTGCTAGCTCCGTTGACGT‑CCCGGTGGGGTGTCATa                                                             >  2:2805932/1‑140 (MQ=255)

                                                                            aTCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTTTTTAATCTTTTTTTGTGTATTGGGACCCTTTGTCACAATGGGGGATAGGTGCTTGGACGT‑ACCCTTTGGGTTTCATaa                                                            >  2:1518997/1‑139 (MQ=255)

                                                                            aTCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTTTATATGCGTTTTTTGTGTATTTGTAGCCATTGGCAGAATGGCGGATATCTCCGTGGACGT‑CCCGTTGGGCGGTCaaaa                                                            >  1:810472/1‑137 (MQ=255)

                                                                                 ggCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTTTTATTGTTTTTTTTTGTATTTTAAGCCGTGGTGAGAATGGAGGATAGCTCCGTTGACGT‑CCCGGTGGGGTGGCATACtttac                                                       >  1:2646042/1‑138 (MQ=255)

                                                                                 ggCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTTTATATGGTTTTGTTTTGTATTTGTATGCTTGGTGAGAATTGAGGATATCTGCGTTGTCGT‑GCCCTTGGGGTGTGATaaattac                                                       >  1:2098240/1‑134 (MQ=255)

                                                                                 ggCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTTGTTATTGGTTTTTTTTGTTTTGGAAGCCCTTGTGAGAATGGAGGATAGCTCCGTGGACGG‑GCCCGTGGGCTGTCATACattga                                                       >  1:2303355/1‑138 (MQ=255)

                                                                                 ggCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTTGTTATTCTTTTGTTTTGTTTTTGTAGCCGTGGTGCGTATTGGGGTTAGCGCCGTTGTCGG‑CCCGGTGGGGTGTGATaaattaa                                                       >  1:3232303/1‑134 (MQ=255)

                                                                                  gCAGTTTGTCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCa                                                      <  1:3356124/140‑1 (MQ=255)

                                                                                  gCAGTTTGCCTTACGGGACTGGCTGCCTTTCACACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCa                                                      <  2:3784442/140‑1 (MQ=255)

                                                                                  gCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGGGTTTGTTTTGTATTGGAAGCCTTGGTCAGAAAGGAGGATAGCTCCGTGGACGT‑CCCGGTGGGCTGTCAGAATTTGCa                                                      >  2:4042032/1‑140 (MQ=255)

                                                                                  gCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCa                                                      <  1:4042032/140‑1 (MQ=255)

                                                                                  gCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCa                                                      <  2:1594360/140‑1 (MQ=255)

                                                                                    aGTTTGCCTTACGGGACTGGCTGCCTGTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACt                                                    <  1:2579301/140‑1 (MQ=255)

                                                                                    aGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACt                                                    <  1:1805881/140‑1 (MQ=255)

                                                                                    aGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACt                                                    <  2:2101408/140‑1 (MQ=255)

                                                                                     gtatGCATTCCGGGAATGGCTGGCTTTCAAACCAAGGGTTTCGAGTCGCTTTTGATCTGCTTTTGTTGTGTATGGGAAGCCTTGGTCAGAATGGAGGCTAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTg                                                   <  2:3766488/137‑1 (MQ=255)

                                                                                     gTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTg                                                   <  2:841218/140‑1 (MQ=255)

                                                                                     gTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTg                                                   <  2:134288/140‑1 (MQ=255)

                                                                                     gTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTg                                                   <  2:3870438/140‑1 (MQ=255)

                                                                                     gTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTg                                                   <  2:2717265/140‑1 (MQ=255)

                                                                                     gTTTGCCTTACGGGAATGGCTGACTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTg                                                   <  2:1503067/140‑1 (MQ=255)

                                                                                      tttGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTTGTTATTGTTTTTTTGTGTATTTGAAGCCTTGGTGAGAAAGGAGGATATGTGCTTGGACGT‑CCCGTTGGGGTGTCATACTTTCCACTGt                                                  >  2:1903419/1‑140 (MQ=255)

                                                                                               cGGGACTGGCTGCCTTTCAAACCAACGGTTTCGTGTCTCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGCTAGCTCCTTTGACGT‑CGCGTTGGGCTGTCATACTTTCCACTGTCTTAATGAc                                         <  1:480460/140‑1 (MQ=255)

                                                                                               cGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGAc                                         <  1:933964/140‑1 (MQ=255)

                                                                                               cGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGAc                                         <  2:3573013/140‑1 (MQ=255)

                                                                                               cGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGAc                                         <  1:1988628/140‑1 (MQ=255)

                                                                                               cGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGAc                                         <  2:3232303/140‑1 (MQ=255)

                                                                                               cGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGAc                                         <  2:319545/140‑1 (MQ=255)

                                                                                               cGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGAc                                         <  2:4119623/140‑1 (MQ=255)

                                                                                               cGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGAc                                         <  1:3609618/140‑1 (MQ=255)

                                                                                               cGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGAc                                         <  1:1319393/140‑1 (MQ=255)

                                                                                               cGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGAc                                         <  1:1703726/140‑1 (MQ=255)

                                                                                               cGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGAc                                         <  2:1951912/140‑1 (MQ=255)

                                                                                                 ggAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGAcct                                       <  1:1226449/140‑2 (MQ=255)

                                                                                                 ggAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTGTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGACTGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGAcct                                       <  1:3998518/140‑2 (MQ=255)

                                                                                                           ccTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATc                             <  2:3662360/140‑1 (MQ=255)

                                                                                                             tttCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCAt                           <  1:2174680/140‑1 (MQ=255)

                                                                                                                                      ttttGATATGCTTTTGTTGTGTTTTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGAc  <  2:2646910/140‑1 (MQ=255)

                                                                                                                                      ttttGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGAc  <  2:1097067/140‑1 (MQ=255)

                                                                                                                                      ttttGATATGCTTGTGTTGGCTATTGGAAGCCTTGGTCAGAATGGAGGATAGCGCCTTGGACGT‑CCCGTTGGGCTGTCAGACTTTCCACGGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGAc  <  2:2411746/140‑1 (MQ=255)

CTTTTTCGGTTTGACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGAC  >  NZ_CP034943/2350709‑2350982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`