Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,425,640 G→T 6.3% intergenic (+4/‑17) EO946_RS12410 → / → EO946_RS12415 DEAD/DEAH box helicase/YqhG family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,425,6400GT 6.3% 462.3 / 2.5 173intergenic (+4/‑17)EO946_RS12410/EO946_RS12415DEAD/DEAH box helicase/YqhG family protein
Reads supporting (aligned to +/- strand):  ref base G (44/109);  new base T (2/17);  total (47/126)
Fisher's exact test for biased strand distribution p-value = 1.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 176 total aligned reads displayed.

CAATTTTGAAGAGCACCTGCACGAAATTTTGTACCATTCAGCAACAGAAGAAGAAATGAAAATAAAAATGGATAACTTAACCTCATTTTTGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCAGGCTATATGACAGTGCAGCTTACAGTTGAAATGGACAAACAGATC  >  NZ_CP034943/2425504‑2425779
                                                                                                                                        |                                                                                                                                           
cAATTTTGAAGAGCACCTGCACGAAATTTTGTACCATTCAGCAACAGAAGAAGAAATGAAAATAAAAATGGATAACTTAACCTCATTTTTGTCTAATAAAAAACATCAGCCTGCTGAAAAAAGAGGATCTTAGACTTTaa                                                                                                                                          >  2:2954123/1‑140 (MQ=255)
 aaTTTTGAAGAGCACCTGCACGAAATTTTGTACCATTCAGCAACAGAAGAAGAAATGAAAATAAAAATGGATAACTTAACCTCATTTTTGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAag                                                                                                                                         <  2:264268/140‑1 (MQ=255)
   ttttGAAGAGCACCTGCACGAAATTTTGTACCATTCAGCAACAGAAGAAGAAATGAAAATAAAAATGGATAACTTAACCTCATTTTTGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAagga                                                                                                                                       <  2:1082665/140‑1 (MQ=255)
   ttttGAAGAGCACCTGCACGAAATTTTGTACCATTCAGCAACAGAAGAAGAAATGAAAATAAAAATGGATAACTTAACCTCATTTTTGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAagga                                                                                                                                       <  1:1842176/140‑1 (MQ=255)
   ttttGAAGAGCACCTGCACGAAATATTGTACCATTCAGCAACAGAAGAAGACATGAAAATAAAAATGGATAACTTAACCTCATTTTTGTCTTATCAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAagga                                                                                                                                       <  1:905599/140‑1 (MQ=255)
   ttgtGAAGAGCACCTGCACGAAATTTTGTACCATTCAGCACCAGAAGAAGAAATGAAAATAAAAATGGATAACTTAACCTCATTTTTGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAagga                                                                                                                                       <  2:134658/137‑1 (MQ=255)
        aaGAGCACCTGCACGAAATTTTGTACCATTCAGCAACAGAAGAAGAAATGAAAATAAAAATGGATAACTTAACCTCATTTTTGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTATCATGTAAGGAGGttt                                                                                                                                  >  2:428197/1‑140 (MQ=255)
        aaGAGCACCTGCACGAAATTTTGTACCATTCAGCAACAGAAGAAGAAATGAAAATAAAAATGGATAACTTAACCTCATTTTTGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGttt                                                                                                                                  >  1:3029039/1‑140 (MQ=255)
        aaGAGCACCTGCACGAAATTTTGTACCATTCAGCAACAGAAGAAGAAATGAAAATAAAAATGGAGAACTTAACCTCATTTTTGTCTTATAAAAAAAAGCAGCCGGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGttt                                                                                                                                  >  1:4334206/1‑140 (MQ=255)
         agagCACCTGCACGAAATTTTGTACCATTCAGCAACAGAAGAAGAAATGAAAATAAAAATGGATAACTTAACCTCATTTTTGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGtttt                                                                                                                                 <  1:2774607/140‑1 (MQ=255)
             cACCTGCACGAAATTTTGTACCATTCAGCAACAGAAGAAGAAATGAAAATAAAAATGGATAACTTAACCTCATTTTTGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTTGCCTGTAAGGAGGTTTTATaa                                                                                                                             >  2:1618801/1‑140 (MQ=255)
             cACCTGCACGAAATTTTGTACCATTCAGCAACAGAAGAAGAAATGAAAATAAAAATGGATAACTTAACCTCATTTTTGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATaa                                                                                                                             >  1:23467/1‑140 (MQ=255)
             cACCTGCACGAAATTTTGTACCATTCAGCAACAGAAGAAGAAATGAAAATAAAAATGGATAACTTAACCTCATTTTTGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTGTTATaa                                                                                                                             >  1:741121/1‑140 (MQ=255)
                  gCACGAAATTTTGTACCATTCAGCAACAGAAGAAGAAATGAAAATAAAAATGGATAACTTAACCTCATTTTTGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTgag                                                                                                                        <  2:2313063/140‑1 (MQ=255)
                  gCACGAAATTTTGTACCATTCAGCAACAGAAGAAGAAATGAAAATAAAAATGGATAACTTAACCTCATTTTTGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTgag                                                                                                                        <  1:1614425/140‑1 (MQ=255)
                  gCACGAAATTTTGTACCATTCAGCAACAGAAGAAGAAATGAAAATAAAAATGGATAACTTAACCTCATTTTTGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTgag                                                                                                                        <  2:3104749/140‑1 (MQ=255)
                       aaaTTTTGTACCATTCAGCAACAGAAGAAGAAATGAAAATAAAAATGGATAACTTAACCTCATTTTTGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGc                                                                                                                   <  2:3442492/140‑1 (MQ=255)
                         aTTTTGTACCATTCAGCAACAGAAGAAGAAATGAAAATAAAAATGGATAACTTAACCTCATTTTTGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCaa                                                                                                                 <  1:1783712/140‑1 (MQ=255)
                          ttttGTACCATTCAGCAACAGAAGAAGAAATGAAAATAAAAATGGATAACTTAACCTCATTTTTGTCTTATAAAAAACAGCAGCCTCCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTGTATAAGTGAGACAGCAag                                                                                                                >  1:970560/1‑140 (MQ=255)
                                    ttCAGCAACAGAAGAAGAAATGAAAATAAAAATGGATAACTTAACCTCATTTTTGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGTATATTAGCATGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGa                                                                                                      >  2:1550172/1‑140 (MQ=255)
                                    ttCAGCAACAGAAGAAGAAATGAAAATAAAAATGGATAACTTAACCTCATTTTTGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAagga                                                                                                                                       >  2:1455636/1‑107 (MQ=255)
                                    ttCAGCAACAGAAGAAGAAATGAAAATAAAAATGGATAACTTAACCTCATTTTTGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAagga                                                                                                                                       <  1:1455636/107‑1 (MQ=255)
                                    ttCAGCAACAGAAGAAGAAATGAAAATAAAAATGGATAACTTAACCTCATTTTTGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGa                                                                                                      >  2:3002575/1‑140 (MQ=255)
                                    ttCAGCAACAGAAGAAGAAATGAAAATAAAAATGGATAACTTAACCTCATTTTTGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGa                                                                                                      >  2:421245/1‑140 (MQ=255)
                                    ttCAGCAACAGAAGAAGAAATGAAAATAAAAATGGATAACTTAACCTCATTTTTGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGa                                                                                                      >  2:421398/1‑140 (MQ=255)
                                    ttCAGCAACAGAAGAAGAAATGAAAATAAAAATGGATAACTTAACCTCATTTTTGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGa                                                                                                      >  1:4270767/1‑140 (MQ=255)
                                    ttCAGCAACAGAAGAAGAAATGAAAATAAAAATGGATAACTTAACCTCATTTTTGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGa                                                                                                      >  2:606129/1‑140 (MQ=255)
                                    ttCAGCAACAGAAGAAGAAATGAAAATAAAAATGGATAACTTAACCTCATTTTTGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGa                                                                                                      >  2:4059322/1‑140 (MQ=255)
                                    ttCAGCAACAGAAGAAGAAATGAAAATAAAAATGGATAACTTAACCTCATTTTTGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGa                                                                                                      >  1:2013449/1‑140 (MQ=255)
                                            cAGAAGACGAAATGAAAATAAAAATGGATAACTTAACCTCATTTTTGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTCAGGAGGTTTTATAAGTGTGACCGCTAGAGGGTCACGAgttggggc                                                                                              >  2:2209065/1‑135 (MQ=255)
                                              gaagaagaaATGAAAATAAAAATGGATAACTTAACCTCATTTTTGTCTTATAAAAAACAGCATCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCtt                                                                                            >  2:4094108/1‑140 (MQ=255)
                                              gaagaagaaATGAAAATAAAAATGGATAACTTAACCTCATTTTTGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCtt                                                                                            >  1:3756128/1‑140 (MQ=255)
                                                       aTGAAAATAAAAATGGATAACTTAACCTCATTTTTGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGttttttt                                                                                   <  1:1618801/140‑1 (MQ=255)
                                                       aTGAAAATAAAAATGGATAACTTAACCTCATTTTTGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGttttttt                                                                                   <  2:1711755/140‑1 (MQ=255)
                                                               aaaaaTGGATAACTTAACCTCATTTTTGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCaaa                                                                           <  1:1512323/140‑1 (MQ=255)
                                                                    tGGATAACTTAACCTCATTTTTGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGATTCGTTTTTTTAAGTAAAAAAGCt                                                                      >  2:3193577/1‑140 (MQ=255)
                                                                           cTTAACCTCATTTTTGTCTTATAAAAAACAGCATCCTGCTGAAAAAAGAGGATCTTAGCCTTTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGGTTCGTTTTTTTCAGGCAAACAGCTGCCAAAt                                                               >  1:2951280/1‑140 (MQ=255)
                                                                             tAACCTCATTTTTTTCTTATAAAAAACAGCAGACTTCTGAAAAAAGATTATATTATCCTTTATGTAGGTTTTATAAGTGAGACAGCAAGTGGTTCTCGATTTTCTGATTCGTTTTTTTCAGGCAAAGAGCTGCCAAAtcg                                                             <  2:2013449/140‑1 (MQ=255)
                                                                             tAACCTCATTTTTGTCTTATAAAAAAGAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAAtcg                                                             <  1:1138684/140‑1 (MQ=255)
                                                                                ccTCATTTTTGTGTTATAAAAAACAGCACCCTGCTGAAACAGGAGGATCTTAGCCTGGAAGGAGGGTTTATAAGTGTGACAGGAAGAGGTTCACGATTTTCTGCTTGGTGTTTTTGTGGGGAAGAGCTGCCaactgggcg                                                          >  2:869140/1‑133 (MQ=255)
                                                                                   cATTTTTTTCTTATAAAAATCTGAATCATTCTGATAATAGAGGTTCTTATCCTGTTTTTATTTTTTATAAGTGAGACAGCAAGTGGTTCACGATTTTGTGCTTCTTTTTTTTCAGGCATACAGCTGCCAAATCGTCGATc                                                       <  2:1786790/140‑1 (MQ=255)
                                                                                   cATTTTTTTATTATAAAAAACAGCATCCTGCTGAAAAAAGAGGATATTAGCCTTTAAGGAGTTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATc                                                       <  2:2863840/140‑1 (MQ=255)
                                                                                   cATTTTTGTCTTATAAAAAACAGCAGGCTCCTGAAAAAATAGGATCTTAGCCTTTAAGGAGGTTTTATAAGTGAGACATCAAGATGTTCCCGATTTTCTGCTTCGTTTTTTTCAGGCTAAAAGCTGCCAAATCGTCGATc                                                       <  1:1835421/140‑1 (MQ=255)
                                                                                   cATTTTTGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATc                                                       <  1:2810870/140‑1 (MQ=255)
                                                                                   cATTTTTGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATc                                                       <  1:84560/140‑1 (MQ=255)
                                                                                   aaTTTTTGTATTATAAAAAACAGCAGCCTGCTTAAAAAAGAGTATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATc                                                       <  2:2195446/139‑1 (MQ=255)
                                                                                     tttttGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCaa                                                     >  1:3645996/1‑140 (MQ=255)
                                                                                     tttttGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCaa                                                     >  2:2544098/1‑140 (MQ=255)
                                                                                         tGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGtt                                                 <  1:1013356/140‑1 (MQ=255)
                                                                                         tGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGtt                                                 <  1:2320016/140‑1 (MQ=255)
                                                                                         tGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGtt                                                 <  1:2545636/140‑1 (MQ=255)
                                                                                         tGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGtt                                                 <  1:4059322/140‑1 (MQ=255)
                                                                                         tGTCTTATAAAAAAAAGCCGCCTTCTGAAAAAAGAGTATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGTGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGtt                                                 <  2:2330637/140‑1 (MQ=255)
                                                                                           tattATAAAAAACATCATCATTATTAAAAAATAGTATCTTATCATTTAATGATTTTTTATAATTGATACAGCAAGCGGTTCACTTTTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCAAAATCGTCGATCAAGGTTCa                                               <  2:3384040/138‑1 (MQ=255)
                                                                                           taataaaaaaaaCAGCCTCCTTCTGAAAAAATTGTATATTTGCATTTAAGGATTTTTTATAAGTGATACAGCAAGATGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCa                                               <  2:3979432/134‑1 (MQ=255)
                                                                                           tCTTATACAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCa                                               <  1:2911621/140‑1 (MQ=255)
                                                                                           tCTTATAACAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCCAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCa                                               <  1:2593671/140‑1 (MQ=255)
                                                                                           tCTTATAAAAAACATCAGCCTGATTAAAAAAGAGTATATTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCa                                               <  2:408094/140‑1 (MQ=255)
                                                                                           tCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTTCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCa                                               <  1:1917230/140‑1 (MQ=255)
                                                                                           tCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCa                                               <  1:2587485/140‑1 (MQ=255)
                                                                                           tCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCa                                               <  1:2960545/140‑1 (MQ=255)
                                                                                           tCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCa                                               <  2:7364/140‑1 (MQ=255)
                                                                                           tCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCa                                               <  1:2981897/140‑1 (MQ=255)
                                                                                           tCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCa                                               <  1:1118516/140‑1 (MQ=255)
                                                                                           tCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCa                                               <  2:3645996/140‑1 (MQ=255)
                                                                                           tCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCa                                               <  1:2693836/140‑1 (MQ=255)
                                                                                           tCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCa                                               <  1:2931732/140‑1 (MQ=255)
                                                                                           tCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCa                                               <  1:505501/140‑1 (MQ=255)
                                                                                           tCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCa                                               <  1:1099039/140‑1 (MQ=255)
                                                                                           tCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTGAGCCTGTAAGGAGGATTTAGAAGTGAGACAGCAAGAGGTACACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAACTCGTCGATCAAGGTTCa                                               <  1:2879633/140‑1 (MQ=255)
                                                                                           tCTTATAAAAAACAGCAGCCTGATTAAAAAATATTATATTACCCTTTATGTATGTTTTTTAAGTTAGACATCATGTGTTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCa                                               <  2:2909725/140‑1 (MQ=255)
                                                                                           tCTTATAAAAAACAGCAGCCTGATGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTCTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCa                                               <  1:1753850/140‑1 (MQ=255)
                                                                                             ttATAAAACACAGCAGCCTGCTGAAAAAAGAGGATATTAGCCTGTAAGGATGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCAgg                                             <  2:606497/140‑1 (MQ=255)
                                                                                             ttATAAAAAACATCAGCCTTATGAAAAAAGATGATCTTAGACTGTAAGGAGGTTTTATAATTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTAAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCAgg                                             <  2:501484/140‑1 (MQ=255)
                                                                                             ttATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCAgg                                             <  1:2296717/140‑1 (MQ=255)
                                                                                             ttATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCAgg                                             <  1:540061/140‑1 (MQ=255)
                                                                                             ttATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCAgg                                             <  1:2851824/140‑1 (MQ=255)
                                                                                             ttATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCAgg                                             <  1:365928/140‑1 (MQ=255)
                                                                                             ttATAAAAAACAGCAGACTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCAgg                                             <  1:3410995/140‑1 (MQ=255)
                                                                                                taACAAACATCATCATTCTGAAAAAAGATGATCTTACCATGTAATGAGTTTTTATAAGTTATACATCAAGATGTTCACGATTTTCTGCTTATTTTTTTTCAGGCAAACAGCTGCCAATTCGTCGATCAAGGTTCAGGCta                                          <  2:3255777/140‑1 (MQ=255)
                                                                                                taAAAAACAGCTGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGTTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCATGCAAACAGCTGCCAAATCGTCGATCAAGGTTCAGGCta                                          <  2:379447/140‑1 (MQ=255)
                                                                                                     aaCAGCAGCCTGCTGAAAAAATAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCAGGCTATATGa                                     <  2:622091/140‑1 (MQ=255)
                                                                                                     aaCAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCAGGCTATATGa                                     >  2:232726/1‑140 (MQ=255)
                                                                                                     aaCAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCAGGCTATATGa                                     >  2:3775011/1‑140 (MQ=255)
                                                                                                     aaCAGCAGCCTCCTGAAAAAATATGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGTTGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCAGGCTATATGa                                     <  2:2293790/140‑1 (MQ=255)
                                                                                                          cagcCTTATTAAAAAATAGTATCTTATCATGTAAGGAGTTTTTATAATTGAGACATCAATATGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCAGGCTATATGACAGTg                                <  2:2948488/140‑1 (MQ=255)
                                                                                                          cagcCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCAGGCTATATGACAGTg                                >  1:882689/1‑140 (MQ=255)
                                                                                                          cagcCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCAGGCTATATGACAGTg                                <  1:3193577/140‑1 (MQ=255)
                                                                                                               tgctgAAACAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCAGGCTATATGACAGTGCAGCt                           <  1:3606925/140‑1 (MQ=255)
                                                                                                               tgctgAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCAGGCTATATGACAGTGCAGCt                           <  1:779645/140‑1 (MQ=255)
                                                                                                               tgctgAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCAGGCTATATGACAGTGCAGCt                           <  1:4307856/140‑1 (MQ=255)
                                                                                                               tgctgAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCAGGCTATATGACAGTGCAGCt                           <  1:118966/140‑1 (MQ=255)
                                                                                                                 atgAAAAAATAGTATCTTATACTTTATGTATTTTTTATAATTTAGACAGCAAGAGTTTCACTATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCAGGCTATATGACAGTGCAGCTTa                         <  2:3136898/139‑1 (MQ=255)
                                                                                                                        aaGATGATATTATCCAGTAAGGAGTTTTTATAATTTAGACAGCAAGTGGTTAACTTTTTTCTTCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCAGGCTATTTGACAGTGCAGCTTACAGTTGa                  <  2:4024407/140‑1 (MQ=255)
                                                                                                                          gagGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCAGGCTATATGACAGTGCAGCTTACAGTTGaaa                <  1:428197/140‑1 (MQ=255)
                                                                                                                          cttgATCTTAGCATTTAAGGAGTTTTTATAATTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGTCATACAGCTGCCAAATCGTCGATCAAGGTTCAGGCTATATTACAGTGCAGCTTACAGTTGaaa                <  1:3147152/137‑1 (MQ=255)
                                                                                                                                cTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCAGGCTATATGACAGTGCAGCTTACAGTTGAAATGGACa          <  1:977702/140‑1 (MQ=255)
                                                                                                                                attAGCCTTTATGGAGTTTTTATAAGTGAGACATCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGTTTCAGGCTATATGACAGTGCAGCTTACAGTTGAAATGGACa          <  2:4142964/139‑1 (MQ=255)
                                                                                                                                        gTAATGATGTTTTATAAGTTATACAGAAAGATCTAAACTATTTTCTTCTTCGTTTTTTTCAGGCAAACAGCTTCCAAATCGTCTATCAAGGTTCAGGATATATGACAGTGCAGCTTACATTTGAAATGGACAAACAGATc  <  2:3420516/140‑1 (MQ=255)
                                                                                                                                        gTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGTGTTTCTGCTTCGTTTTTTTCATGCAAACAGCTGCCTAATCGTCGATCACGGTTCAGGCTATATGAGAGTGCAGCTTACAGTTGAAATGGACAAACAGATc  <  1:1025697/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                           
CAATTTTGAAGAGCACCTGCACGAAATTTTGTACCATTCAGCAACAGAAGAAGAAATGAAAATAAAAATGGATAACTTAACCTCATTTTTGTCTTATAAAAAACAGCAGCCTGCTGAAAAAAGAGGATCTTAGCCTGTAAGGAGGTTTTATAAGTGAGACAGCAAGAGGTTCACGATTTTCTGCTTCGTTTTTTTCAGGCAAACAGCTGCCAAATCGTCGATCAAGGTTCAGGCTATATGACAGTGCAGCTTACAGTTGAAATGGACAAACAGATC  >  NZ_CP034943/2425504‑2425779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: