Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,502,242 T→G 18.3% T274P (ACG→CCG)  hrcA ← heat‑inducible transcriptional repressor HrcA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,502,2420TG18.3% 232.2 / 8.6 139T274P (ACG→CCG) hrcAheat‑inducible transcriptional repressor HrcA
Reads supporting (aligned to +/- strand):  ref base T (55/52);  new base G (30/2);  total (85/54)
Fisher's exact test for biased strand distribution p-value = 6.87e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 146 total aligned reads displayed.

AATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAAC  >  NZ_CP034943/2502107‑2502377
                                                                                                                                       |                                                                                                                                       
aatTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCgtgtgt                                                                                                                                     <  1:2943130/140‑1 (MQ=255)
 atTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGtgtgtg                                                                                                                                    >  2:2165191/1‑140 (MQ=255)
 atTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGtgtgtg                                                                                                                                    <  2:3574633/140‑1 (MQ=255)
 atTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTCCCGATTTTAATCGAAATCCCCGtgtgtg                                                                                                                                    >  1:201543/1‑140 (MQ=255)
  ttCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCCTCTCTTCATAGTCGTTTTCTTTCCCGATGTTAATCGAAATCCCCgtgggtgg                                                                                                                                   >  2:3290498/1‑140 (MQ=255)
  ttCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTACCGATTTTAATAGAAAAACCCGTGTGTgg                                                                                                                                   >  1:1482670/1‑140 (MQ=255)
  ttCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTATTCATAGTCGTGTTCTTTCCAGATTTTAATAGAAATACACCTGTgggg                                                                                                                                   >  1:2408365/1‑140 (MQ=255)
      tGCGGGTCGGGCCGATAATCGCAATTGAGCCGATCTGCTTCTGGTCTACGGAATAAGAAGCCGTAATCAGACTGCAATTTTCCATCTCTTCATAGTCGTGTTGTTTTCCGATTTTAAAAGAAATTCCCGGGTGCGGGGAt                                                                                                                               >  1:2878835/1‑140 (MQ=255)
       gCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATAGAAATCCCCGTGTGTGGGGAtt                                                                                                                              >  2:192517/1‑140 (MQ=255)
          ggTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTCCCCATTTTAATCGAAATCCCCGGGTGTGGGGATTGaa                                                                                                                           >  1:1661450/1‑140 (MQ=255)
            tCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGACTAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTATTTCCCGATTTTAATCCAAAACCACGTGTGTGGGGATGGCACg                                                                                                                         >  1:2529574/1‑140 (MQ=255)
            tCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACg                                                                                                                         >  2:393062/1‑140 (MQ=255)
            tCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACg                                                                                                                         >  2:63034/1‑140 (MQ=255)
            tCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACg                                                                                                                         <  2:201543/140‑1 (MQ=255)
                     tgatCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTaaa                                                                                                                >  2:1517832/1‑140 (MQ=255)
                     tgatCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTaaa                                                                                                                <  1:3837095/140‑1 (MQ=255)
                     tgatCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTAATAGTCGGGTTCTTTCCCGAGTTGAATCGACATCCGCGGGTGGGGGGATTGACCGACGTTTaat                                                                                                                >  2:3629804/1‑139 (MQ=255)
                     tgatCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTGTCCATCTCTTCATAGTCGTGTTCTTTACCGATTTTAATCGAAATGCCCGTGGGGGGGGAGTGAACGAGCTGTaaa                                                                                                                >  2:3353000/1‑140 (MQ=255)
                      gatCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTaaaa                                                                                                               <  1:4017231/140‑1 (MQ=255)
                         cGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGCGCTTTAAAACAt                                                                                                            >  2:1882807/1‑140 (MQ=255)
                         cGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACAt                                                                                                            >  2:1499266/1‑140 (MQ=255)
                              ttGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAAAGAGCTTTAAAACAACCGGt                                                                                                       >  2:2471739/1‑140 (MQ=255)
                               tGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGtt                                                                                                      <  2:479748/140‑1 (MQ=255)
                                   ccGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTGTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCttt                                                                                                  <  1:1690535/140‑1 (MQ=255)
                                   ccGATCTGCGTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCttt                                                                                                  <  1:2068691/140‑1 (MQ=255)
                                     gATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCttttt                                                                                                <  1:1743574/140‑1 (MQ=255)
                                     gATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCttttt                                                                                                <  1:3455724/140‑1 (MQ=255)
                                      aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTGCCGATTTTAATAGAAATACCCGGGTGTGGGGCTTGAACGAGAGTTAAAAAATCCTGTTGTTTTTc                                                                                               >  2:3923132/1‑140 (MQ=255)
                                      aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTTATAGAAATACCCGGGTGTGGGGATTGGACGAGCGTTTAAACATACGGTTGttttta                                                                                               >  2:1134289/1‑139 (MQ=255)
                                      aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTTTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTc                                                                                               >  2:4255999/1‑140 (MQ=255)
                                      aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTTAACGAGCTTTAAAACATCCGTTTCttttta                                                                                               >  2:3095249/1‑139 (MQ=255)
                                      aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGGTGGTTTTc                                                                                               >  2:2484658/1‑140 (MQ=255)
                                      aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACAACCTGTTCTTTTTc                                                                                               >  2:334561/1‑140 (MQ=255)
                                      aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGGGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCttttta                                                                                               >  2:3536059/1‑139 (MQ=255)
                                      aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATAGAAATCCCCGTGTGGGGGGATTGAACGAGCTTTAAAAAATCCTGTTCTTTTTc                                                                                               >  2:2639564/1‑140 (MQ=255)
                                      aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATAGAAAAACCCGGGTGTGGGGATTGAACGAGCTTTAAAAAATCCTGTTCttttta                                                                                               >  1:1689884/1‑139 (MQ=255)
                                      aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTCCCGATTTTAATCGAAATCCCCGGGTGTGGGGAATGAACGAGCTTTAAAACATCCTGTTAGTTTTc                                                                                               >  2:160587/1‑140 (MQ=255)
                                      aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCAGCTCTTCATAGTCGGTTTCTTTCCCGATTTTATTCGAAATCCCCGTGTGTGGGGCTTGTACGAGCGTTAAAACATCCTGTTCTTTTTc                                                                                               >  2:3100505/1‑140 (MQ=255)
                                       tCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTCCCGATTTTAATCGAAAACCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATGCTGTTCTTTTTCa                                                                                              >  2:947985/1‑140 (MQ=255)
                                           cTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAAGAGCCTGTTCTTTTTCAATTa                                                                                          >  2:4251073/1‑140 (MQ=255)
                                                   cAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATgagaga                                                                                  <  2:4111602/140‑1 (MQ=255)
                                                   cAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATgagaga                                                                                  <  2:3724284/140‑1 (MQ=255)
                                                     aCGTAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATCTTCGTTTTCTTTCCCTATTTTAATCGAAATCCCCGTGTGTGGGGATTGACCGAGATTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAg                                                                                <  1:1829626/140‑1 (MQ=255)
                                                     aCGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAg                                                                                <  2:75743/140‑1 (MQ=255)
                                                     aCGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCGTTGCCGATTTTAATAGAAATCCCCGGGTGTGGGGATTGAACGAGCTTTAAAACATCCGGTTATTTTTGAATTAATGTGAGAAg                                                                                >  1:44002/1‑140 (MQ=255)
                                                          aTAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTACTCGAAATCCCCGGGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGAcc                                                                           <  2:570262/140‑1 (MQ=255)
                                                          aTAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCGGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGAcc                                                                           <  2:2089908/140‑1 (MQ=255)
                                                          aTAACAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCATTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGAcc                                                                           <  2:4007807/140‑1 (MQ=255)
                                                           tAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTGTTTCCCGATTTTAATCGAAATACGCCGGGGGGGGGATTGAAAGAGCTTTAAAAGATGCTGTTCTTTTTCAATTTTTTATTGAAGCGACCg                                                                          >  2:3627721/1‑140 (MQ=255)
                                                                 gCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTTATAGAAATCCCCGGGTGTGGGGCTTTTAGGAGCTTTAAAACATCCGGTTGTTTTTGAATTAATGAGTGAAGCGACCGAActct                                                                    >  1:3087271/1‑140 (MQ=255)
                                                                 gCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTGTTTGCCGATTTTAATCGAAAAACCCGGGTGTGGGGTTTTAACGGGCTTTAAAACATCCGGTTGTTTTTGAATTAAATATAGGATCGACCGAAcgct                                                                    >  1:1709644/1‑140 (MQ=255)
                                                                 gCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTGTTTCCCGCTTTTAATCGAAATACCCGGGTGTGGGGATTGTACGAGCTTTAAAAAATCCTGTTCTTTTTAAATTAATTAGAGAAGCGACCGAAatct                                                                    >  1:767567/1‑140 (MQ=255)
                                                                 cacGTAATCACACTGAAGTTTTACATATCAAAATAGTCGTTTTATTTCCCGAATTTAATACAAATACCCCTGTTTGGGGATTTAAAGAGCTTTTAAAAATGCTGTTGTTTTTAAATTAATGAGAGAAGCGACCGAActct                                                                    >  2:449318/3‑140 (MQ=255)
                                                                  ccGTAATCAGACTGCAGTTTTCCATATCTTCATAGTCGTATTCTTTCCCGATTGTAATCGAACTCCCCGTGTGTGGGGATTGAACGAGCTTTAACACATCCTGGTCATTTTCAATGATTGAGAGAAGCGACCGAACTCTg                                                                   <  1:358728/140‑1 (MQ=255)
                                                                   cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTTTTTGCCGATTTTAATAGAAATACCCGGGGGTGGGGAATTAACGAGCTTTAAAAAAGCCTGTTGTTTTTGAATTAATTATAGTAGCGACAGAAcgcggg                                                                  >  1:3782512/1‑135 (MQ=255)
                                                                   cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCGATTTTAATCGAAATCCCCGTGGGGGGGGGTTGAACGAGGTTTAAAACATCCTGTTCTTTTGCAATTAAGGCGCGCAGCGACCGAACTCTgg                                                                  >  2:4077793/1‑140 (MQ=255)
                                                                   cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTGCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTTAACGGGCTTTTAAACATCCTGTTCATTTTAAATTAATGATAGAAGCGACCGAActcttg                                                                  >  2:349194/1‑138 (MQ=255)
                                                                   cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAAACCCCGGGTGTGGGGATTGAACGAGCTTTAAAAAATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTgg                                                                  >  2:125823/1‑140 (MQ=255)
                                                                   cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTACCGATTTTAATCGAAATCCCCGGGTGTGGGGATTGAACGAGCTTTAAAACCTCGGGTTCTTTTTCAATTAATGAGAGAAGCGACCGCACTCTgg                                                                  >  2:2809812/1‑140 (MQ=255)
                                                                   cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTACCGATTTTAATAGAAATCCCCGGGGGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAAAGAGAGAAGCGACCGAAATCTgg                                                                  >  2:3320783/1‑140 (MQ=255)
                                                                   cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTGTTTCCCGATTTTAATCGAAAAACCCCTGTGTGGGGCTTTAAAGAGCTTTTAAACATCCTGTTGTTTTTCAATTAATGTGAGAAGCGACCGAAcgatgg                                                                  >  1:3779858/1‑135 (MQ=255)
                                                                   cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCGTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACAGGCGGTTATTTTTCAATTAATGTGAGCAGCGACCGAAcgcggg                                                                  >  2:3500435/1‑135 (MQ=255)
                                                                    gtacacAGACTGCCGTTTTCCATCTCTGCATCGCCGTTTTCGTTCCCGATTTTCCTCGACATCCCCGTGTGTGGGGTTTGACCGAGCTTTAAAACTTCCTGTTCTTTTTCAATGAATGAGAGAAGCGACCGAACTCTGGt                                                                 <  2:797477/135‑1 (MQ=255)
                                                                     tAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTg                                                                <  1:63034/140‑1 (MQ=255)
                                                                      aaTCAGACTGCAGTTTTCCATCTCTTCATAGTCCTTTTCTTTCCCGTTTTTAATAGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTTTTCTTTTTCTATTAATGAGAGAAGCGACCGAACTCTGGTGa                                                               <  2:929338/140‑1 (MQ=255)
                                                                        tCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGata                                                             <  1:1517832/140‑1 (MQ=255)
                                                                        tCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGata                                                             <  1:334555/140‑1 (MQ=255)
                                                                        tCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGata                                                             <  1:3158112/140‑1 (MQ=255)
                                                                        tCAGACTGCAGTTTTCCATCTCTGCATCGTCTTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGATTTAAAAAATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGata                                                             <  1:3923132/140‑1 (MQ=255)
                                                                             cTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATg                                                        <  2:690333/140‑1 (MQ=255)
                                                                             cTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATg                                                        <  2:690762/140‑1 (MQ=255)
                                                                               gCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATACCCGGGTGTGGGGATTGAAACAGCTTTAAAAAAGACGGTTATTTTTAAATTAATGAGAGAAACCAACCAACACTGGTGATATAATGGa                                                      >  2:1139155/1‑140 (MQ=255)
                                                                                cAGTTTTCCATCTCTTCATAGTCGTGTTCTTTCCCGATTTTAATCGCAATCCCCCTGTGTGGGGATTGAACGAGCGTTAAAACATCCTGTTGTTTTTCAATTAATGGGAGAAGCGCCCGAAATCGGGTGATATCATGGaa                                                     >  2:2688539/1‑140 (MQ=255)
                                                                                     ttCCATCTCTTCATAGTCGTTTTGTTTGCCGATTTTTATCGAAATACCCGGGTGTGGGGGTTTTACGTGCGTTAAAACATCCTGTTGTTTTTGAATTAAAGGTAGAAGCGACCGAACTCTGGTGTTATAATTGaaatacg                                                >  1:2060183/1‑135 (MQ=255)
                                                                                      tCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTACTCGAAAGCCCCGTGGGTGGGGATTGACCGAGCTTTAAAACATCCTGTTCTTTTGCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCgg                                               <  1:319844/140‑1 (MQ=255)
                                                                                       ccATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGc                                              <  1:1481461/140‑1 (MQ=255)
                                                                                        cATCTCTTCATAGTCGTTTTGTTTCCCCATTTTAATAGAAATACCCCTGGGGGGGGATTTAACCAGCGTTAAAAAATCCTGTTCTTTTTAAATTAATTAGATAACCGACCCAACTCTGGTGGTAACATGGTAcaaccgcg                                             >  1:350634/1‑133 (MQ=255)
                                                                                        cATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGGGTGTGGGGATTGAACGTGCTTTAAAACAGCCTGTTCTTTTTCAATTAATGAGAGTAGCGACCGAACTCTGGTGTTATCATGGTACTCCGGCt                                             >  2:2275366/1‑140 (MQ=255)
                                                                                        cATCTCTTCATAGTCGTTTTCTTTACCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATACTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGCCt                                             >  2:3579253/1‑140 (MQ=255)
                                                                                        cATCTCTTCATAGTCGTGTTGTTTCCCCCTTTTAAACGAAATCCCCGTGTGTGGGGATTGAACGAGCGTTAAAACATCCGGTTGTTTTTGAAATAATTTGAGAAGCGACCGTACTATGGTGATATCAATGAACACCGGcg                                             >  1:1034119/1‑139 (MQ=255)
                                                                                        cATCTCTTCATAGTCGTGTTCTTTCCCGATTTTAATCGAAATCCCCGGGTGGGGGGTTTGGACGCGGGTTAAAAACTCCTGTTGTTTTTCAATTAATGTGAGTAGGGACCGAACGCTGGGGGTATCATGGAACTCCGGCt                                             >  1:4187934/1‑140 (MQ=255)
                                                                                         aTCTCTTCATCTTAGTTTTCTTTCCCGTTTTAAATCTAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTGCAATTAATGAGAGAAGCGACCGATCTCTGGTGATATCATGGAACTCCGGcag                                            <  1:3627721/140‑3 (MQ=255)
                                                                                          tctcTTCATAGTCGTGTTGTTTGCCGATTTTTATAGAAATACCCGTGTGTGGGGATTTAAAGAGCTTTAAAAAATCCCGTTCTTTTTTAAATAATTTTGTAATCGACCGAACTCGGGTGGTATCATGGAACTCCGGCTgg                                           >  1:3308135/1‑140 (MQ=255)
                                                                                            tctTCATCGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGtt                                         <  1:115098/140‑1 (MQ=255)
                                                                                            tctTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAACCCCCGGGTGTGGGGATTGAACGAGATTTAAAACATCCTGTTCTTTTTCAATTACGGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGtt                                         <  1:115094/140‑1 (MQ=255)
                                                                                             ctTCATAGTCGTGTTCTTTCCCGATTTTAATAGAAAACCCCGGGTGTGGGGATTGAAAGAGCGTTAAAACATCCGGTTCTTTTTCAATTAAAGAGAGAAGCGACCGAACTCTGGTGGTATCATGGAACTCCCGCGGGtta                                        >  1:3076770/1‑139 (MQ=255)
                                                                                              ttCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCa                                       <  2:4007697/140‑1 (MQ=255)
                                                                                                     tCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGACCTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATAtt                                <  2:1550742/140‑1 (MQ=255)
                                                                                                     tCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGGGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTATTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCCGCTGGTTCAGCATAtt                                >  2:2331184/1‑140 (MQ=255)
                                                                                                     tCGTTTTCTTTACCGATTTTAATCGAAATACCCGTGTGTGGGGATTTAACGAGCTTTAAAACATCCTGTTCTTTTTAAATTAATGATAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATAtt                                >  2:1451537/1‑140 (MQ=255)
                                                                                                     tCGGTTTGTTTCCCGATTTTAATAGAAATCCCCGTGGGTGGGGATTGAACGAGCGTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATAtt                                >  2:1013439/1‑140 (MQ=255)
                                                                                                        ttttCTTTCCCGATTTTAATCGAAAAACCCGTGTGTGGGGATTGAACGAGCTTTAAAAAATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGAt                             >  2:3646611/1‑140 (MQ=255)
                                                                                                               tcCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTccg                      >  2:2899800/1‑140 (MQ=255)
                                                                                                                         aaTCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACa            >  2:4135197/1‑140 (MQ=255)
                                                                                                                         aaTCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGAGTTTTCCGCCGAAAAACa            >  2:1817045/1‑140 (MQ=255)
                                                                                                                           tCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAAt          >  1:401241/1‑140 (MQ=255)
                                                                                                                                aTCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTc     <  2:2566490/140‑1 (MQ=255)
                                                                                                                                aTCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATACTTTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTc     <  1:1828178/140‑1 (MQ=255)
                                                                                                                                aTCCCCGTGTGTGGGGATCGAACGCGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGCAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATATTTCCGCCGAAAAACAATTTTTc     <  1:2859394/140‑1 (MQ=255)
                                                                                                                                   cccGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTACAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAAc  <  2:2024195/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                       
AATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAAC  >  NZ_CP034943/2502107‑2502377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: