Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,680,400 A→T 10.3% V417D (GTT→GAT)  EO946_RS13875 ← LysM peptidoglycan‑binding domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,680,4000AT10.3% 378.9 / 4.8 164V417D (GTT→GAT) EO946_RS13875LysM peptidoglycan‑binding domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (66/68);  new base T (6/10);  total (79/85)
Fisher's exact test for biased strand distribution p-value = 4.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 180 total aligned reads displayed.

AGCCGCTTCTTTCTCTTCATGACGTAGTGCAGGCTCGGCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACG  >  NZ_CP034943/2680261‑2680539
                                                                                                                                           |                                                                                                                                           
aGCCGCTTCTTTCTCTTCCTGACGTAGTGCAGGCTCGGCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCaa                                                                                                                                             <  1:2187708/140‑1 (MQ=255)
aGCCGCTTCTTTCTCTTCATGACGTAGTGCAGGCTCGGCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCaa                                                                                                                                             >  2:20284/1‑140 (MQ=255)
aGCCGCTTCTTTCTCTTCATGACGTAGTGCAGGCTCGGCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCaa                                                                                                                                             <  1:3074650/140‑1 (MQ=255)
aGCCGCTTCTTTCTCTTCATGACGTAGTGCAGGCTCGGCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCaa                                                                                                                                             <  1:2388235/140‑1 (MQ=255)
aGCCGCTTCTTTCTCTTCATGACGTAGTGCAGGCTCGGCATGCTACGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGCGCTCAGCGTGCTGCGGTTGAGCCGGTTATTTGTCTTATTGACGCAGAGCCGGCTCaa                                                                                                                                             >  1:2493214/1‑140 (MQ=18)
  ccGCTTCTTTCTCTTCATGACGTAGTGCAGGCTCGGCTTGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTGCTCTTCTTGACGGAGTGCGGGCTCAGCg                                                                                                                                           >  2:389883/1‑140 (MQ=255)
  ccGCTTCTTTCTCTTCATGACGTAGTGCAGGCTCGGCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACg                                                                                                                                           <  1:3380883/140‑1 (MQ=255)
  ccGCTTCTTTCTCTTCATGACGTAGTGCAGGCTCGGCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACg                                                                                                                                           >  2:4232648/1‑140 (MQ=255)
  ccGCTTCTTTCTCTTCATGACGTAGTGCAGGCTCGGCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGACTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACg                                                                                                                                           <  1:2603257/140‑1 (MQ=255)
          ttctCTTCATGACGTAGTGCAGGCTCGGCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCgg                                                                                                                                   <  1:4225572/140‑1 (MQ=255)
                   tGACGTAGTGCAGGCTCGGCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGc                                                                                                                          <  2:901084/140‑1 (MQ=255)
                   tGACGTAGTGCAGGCTCGGCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGc                                                                                                                          <  2:284046/140‑1 (MQ=255)
                   tGACGTAGTGCAGGCTCGGCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGc                                                                                                                          <  2:331663/140‑1 (MQ=255)
                             cAGGCTCGGCCTGCTGCGATTGAGCCGCTTCTTCCGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGct                                                                                                                <  2:3749915/140‑1 (MQ=255)
                               ggCTCCGGATGCTCCGGTTGAGCAGCTTCTTGCGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGcttc                                                                                                              <  2:3669742/140‑1 (MQ=14)
                                gCTCGGCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCGGCTTCTGTCTCTTCTTGACGGAATGCCGGCTCCACGTGCTCCGGTAGAGCCGCTTCTTCAGcttct                                                                                                             >  1:3185542/1‑140 (MQ=18)
                                gCTCGGCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGcttct                                                                                                             >  1:124886/1‑140 (MQ=255)
                                    ggCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTGCAGCTTCTTGAc                                                                                                         >  2:2538221/1‑140 (MQ=255)
                                     gCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCATCGTGCTCCGGTTGAGCCGCTTCTGATGCTTCTTGACg                                                                                                        >  2:3825716/1‑140 (MQ=255)
                                     gCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACg                                                                                                        >  1:2362798/1‑140 (MQ=255)
                                     gCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTGCGGTTGAGCCGCTTCTTGTGCTTCTTGACg                                                                                                        >  1:3341445/1‑140 (MQ=255)
                                     gCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTTCTTCTTGACg                                                                                                        >  1:3419506/1‑140 (MQ=255)
                                     gCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGAc                                                                                                         <  1:4249499/139‑1 (MQ=255)
                                     gCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACg                                                                                                        >  2:1456725/1‑140 (MQ=255)
                                     gCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACg                                                                                                        >  1:4142887/1‑140 (MQ=255)
                                     gCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACg                                                                                                        >  2:2954861/1‑140 (MQ=255)
                                     gCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTATGCTTCTTGACg                                                                                                        >  1:2229559/1‑140 (MQ=255)
                                     gCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTGTCTCTTCTTGACGGTGGGGGGGCTCAACGTGCTGCGGTTGAGCCGCGTCTTCTGCTTCTTGACg                                                                                                        >  1:798338/1‑140 (MQ=17)
                                     gCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCGTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCAGGTTGAGCCGCTTCTTCTTCTTCTTGaag                                                                                                        >  1:3536082/1‑138 (MQ=255)
                                     gCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCGTTATGTTGATGGGGGAGTGGGAGCTCATAGTCCTCCGGGGGAGCCGCTGGTGCTTCTTCTTGACg                                                                                                        >  1:210042/1‑140 (MQ=12)
                                      cgtGCTCCGTGTGCTCCGATTCTTGCGCTTAATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGGCGCTTCTTTCGCGTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACgg                                                                                                       <  2:760427/138‑1 (MQ=14)
                                      cccgCTCCGCTTGACCCGCGTCGTCCGCGTCATGACCGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCGACTTTCTCTACTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACgg                                                                                                       <  2:3135891/137‑1 (MQ=17)
                                      cATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACgg                                                                                                       <  2:685759/140‑1 (MQ=255)
                                      cATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACgg                                                                                                       <  2:1075260/140‑1 (MQ=255)
                                      cATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACgg                                                                                                       <  2:1582925/140‑1 (MQ=255)
                                      cATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACgg                                                                                                       <  2:3795156/140‑1 (MQ=255)
                                          cTCCGATTGAGCCGCTTCTTCCGCTTCATGAGGGAGTGCGGGCTCAGCGTGCTCCGGTTGTGCCGCTTCTATCTCTTCTTGACGGAGTTCGGGCTCTCCGTGCTCCGGTTGTGGCGCTTCTGGTGCTTGTTGACGGAGTg                                                                                                   >  1:4295507/1‑140 (MQ=18)
                                          cTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTTAGCCGCTTCTTGTGCTTCTTGACGGAGTg                                                                                                   >  1:1094254/1‑140 (MQ=255)
                                          cTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACgg                                                                                                       <  2:543501/136‑1 (MQ=255)
                                          cTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACgg                                                                                                       >  1:543501/1‑136 (MQ=255)
                                          cTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTg                                                                                                   <  1:1456725/140‑1 (MQ=255)
                                          cTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTg                                                                                                   <  1:937468/140‑1 (MQ=255)
                                          cTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTg                                                                                                   >  2:2917179/1‑140 (MQ=255)
                                          cTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCGTGATGTTGTTGAGGGTGTGCCGGCTCATCGTGATCCGGTTGAGCCGCGTCTTCTGCTTCTTGagcgactg                                                                                                   >  2:1277568/1‑133 (MQ=14)
                                          cTCCGATTGAGCCGCTTCGTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTg                                                                                                   <  1:1462563/140‑1 (MQ=255)
                                              gATTGTGGCGCTTGTTCGGTTTCTTGCCGGCGTTCTTGCTCAGATTCCTCCGGTTGAGCCGCTTCTTTCTCTTTTTGACGGATTGCGGGCTCAACGTGCTCCGGTTGAGCCGTTTCTTCTGCTTCTTGACGGAGTGCggg                                                                                               <  1:2039442/140‑1 (MQ=17)
                                               cttGAGCCGCTTCTTCCGCTTCCTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGc                                                                                              <  2:1746425/139‑1 (MQ=255)
                                               cttGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGc                                                                                              <  2:208743/139‑1 (MQ=255)
                                               aTTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGc                                                                                              <  1:2419738/140‑1 (MQ=255)
                                               aTTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTATGCTTCTTGACGGAGTGCGGGc                                                                                              >  1:928009/1‑140 (MQ=255)
                                               aTTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTATCTCTTCTTGACGTGGTGACGGCTCAAAGTGCTCCGGTTGAGCCGCATCATAAGCTTCATGAAGGAATgacggc                                                                                              >  1:2740043/1‑135 (MQ=14)
                                                     ccGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGtt                                                                                                                                 >  1:3193377/1‑99 (MQ=255)
                                                           cttcCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTcc                                                                                  <  2:691284/140‑1 (MQ=255)
                                                           ctcccGCTCCATGACGGAGAGCGCGCTCACCGAGCTCCCGATGACCCGCTTCTTCGTCGTCTTGAGCGACAGCTGGCTCAACGTGCTACGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTcc                                                                                  <  2:3697768/137‑1 (MQ=9)
                                                           cgtcCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCTGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCTGGCTCATCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTcc                                                                                  <  2:1189801/138‑1 (MQ=255)
                                                             tcCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCgg                                                                                >  1:2910012/1‑140 (MQ=255)
                                                                              tGCTGGCTCAGCGTGCTCCGGTTGAGCCGATTCTTTCTCTTCTTGACGGAGTGCGGGCTCATCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTg                                                               <  2:1291209/140‑1 (MQ=255)
                                                                                cGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCg                                                                                        >  1:2443474/1‑113 (MQ=255)
                                                                                cGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGct                                                             >  2:1862459/1‑140 (MQ=255)
                                                                                cGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCGTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCg                                                                                        <  2:2443474/113‑1 (MQ=255)
                                                                                   gCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCATCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGcttct                                                          <  1:998708/140‑1 (MQ=255)
                                                                                      cAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTg                                                                                                   >  2:1400718/1‑96 (MQ=255)
                                                                                      cAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTg                                                                                                   <  1:1400718/96‑1 (MQ=255)
                                                                                      cAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCGTATGCTTCTTGAGGGAGTGCGGGCTCAGCGTGCTCCGGGTGAGCCGCTTCGTCTGCGTCTTGa                                                       >  2:1062552/1‑140 (MQ=17)
                                                                                      cAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGGGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGa                                                       <  1:1913460/140‑1 (MQ=255)
                                                                                        gCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACg                                                     >  2:4066496/1‑140 (MQ=255)
                                                                                        gCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACg                                                     >  1:488634/1‑140 (MQ=255)
                                                                                         cGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACgg                                                    <  2:2667285/140‑1 (MQ=255)
                                                                                         cGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACgg                                                    <  1:133024/140‑1 (MQ=255)
                                                                                            gCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGc                                                                                     >  1:2437223/1‑104 (MQ=255)
                                                                                            gCTCCGGTTGAGCCGCGTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGc                                                                                     <  2:2437223/104‑1 (MQ=255)
                                                                                             gtCCGGTTGAGCCGCGTCTTTCTCTTATTGACGTAGTGCAGGCTCTGCCTGCTCCGTTTGAGCCGCTTCTTCCGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATg                                                <  1:1099731/139‑1 (MQ=11)
                                                                                             cTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCATCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATg                                                <  2:784141/140‑1 (MQ=255)
                                                                                             cTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGGGTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTGGGCGTCTTGACGGAATg                                                >  1:1676115/1‑140 (MQ=17)
                                                                                             cTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTGTGCGTCTTTACGGAATg                                                >  2:1993487/1‑140 (MQ=255)
                                                                                             cTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATg                                                >  1:2983186/1‑140 (MQ=255)
                                                                                             cTCCGGTTGAGCCGCTTCTTGCTCTTCTTCACGGAGTGCGGGCGCAACGTGCTGCGGTTGAGCCGCTTCTTCTGCGTCTTTACGGAGTGCGGGCTCAGCGTGCTCCGGGTGAGCCGGTACTACATCATCCTGACGGactc                                                >  2:238603/1‑137 (MQ=14)
                                                                                             cTCCGGTTGAGCCGCGTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATg                                                <  2:2910012/140‑1 (MQ=255)
                                                                                                 ggTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTGTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCGTCTTCTGCTTCTTGACGGAATGCAgg                                            >  1:4100741/1‑140 (MQ=255)
                                                                                                 ggTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTTCGGGCTCAGCGTGCTCCGGTTGAGCCGCGTCTTATTCTTCTTGACGGAATGCAgg                                            >  1:734955/1‑140 (MQ=255)
                                                                                                  gTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTATTCTGCTTCTTTACGGAATGGAggg                                           >  1:2327220/1‑139 (MQ=255)
                                                                                                  gTTGAGCCGCTTCTTTCTCTTCGTGACGGAGTGCGGGCTCACCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGc                                           <  1:3949642/140‑1 (MQ=255)
                                                                                                   ttGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACgg                                                    <  1:3294394/130‑1 (MQ=255)
                                                                                                   ttGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACgg                                                    >  2:3294394/1‑130 (MQ=255)
                                                                                                     gAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCa                                        <  2:3054274/140‑1 (MQ=255)
                                                                                                        ccGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGcttct                                                          <  2:2430922/119‑1 (MQ=255)
                                                                                                        ccGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCCGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCg                                     <  2:1458911/140‑1 (MQ=255)
                                                                                                        ccGCTTCTCTCTCTTCGTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCg                                     <  2:3388823/140‑1 (MQ=255)
                                                                                                         cGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGt                                    <  1:594956/140‑1 (MQ=255)
                                                                                                                               aGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGAc                                                      >  2:564188/1‑100 (MQ=255)
                                                                                                                               aGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGGTCCGGTTGAGCTGCttcttt              >  1:65801/1‑140 (MQ=255)
                                                                                                                               aGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCttcttt              >  1:4052006/1‑140 (MQ=255)
                                                                                                                               aGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCttcttt              >  1:3896874/1‑140 (MQ=255)
                                                                                                                               aGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCttcgtt              >  1:67355/1‑140 (MQ=255)
                                                                                                                                 tGCAGGCTCGGCATGCTCCGGTTGAGCCGCTTCTTCAGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTATGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCAGCttctttat            >  1:313873/1‑138 (MQ=11)
                                                                                                                                           aCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGACTGCAGGCACAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACg  >  2:4270912/1‑140 (MQ=255)
                                                                                                                                           aCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCggttctgttct  >  2:4178848/1‑135 (MQ=255)
                                                                                                                                           aCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACg  >  2:1370703/1‑140 (MQ=255)
                                                                                                                                           aCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACg  >  1:1817610/1‑140 (MQ=255)
                                                                                                                                           aCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACg  >  1:770837/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
AGCCGCTTCTTTCTCTTCATGACGTAGTGCAGGCTCGGCATGCTCCGATTGAGCCGCTTCTTCCGCTTCATGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACG  >  NZ_CP034943/2680261‑2680539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: