Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,775,852 A→G 5.4% G145G (GGT→GGC EO946_RS14350 ← TVP38/TMEM64 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,775,8520AG 5.4% 619.8 / 16.8 186G145G (GGT→GGCEO946_RS14350TVP38/TMEM64 family protein
Reads supporting (aligned to +/- strand):  ref base A (122/54);  new base G (6/4);  total (128/58)
Fisher's exact test for biased strand distribution p-value = 5.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-01

Only 100 of 191 total aligned reads displayed.

TATACAAAGCGAGCACCATAAAGAAAGCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAA  >  NZ_CP034943/2775713‑2775986
                                                                                                                                           |                                                                                                                                      
tataCAAAGCGAGCCCCATAAAGAAAGCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTCACATTGGAAAGa                                                                                                                                        >  1:922505/1‑140 (MQ=255)
tataCAAAGCGAGCACCATAAAGAAAGCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGa                                                                                                                                        >  1:1331864/1‑140 (MQ=255)
  taCAAAGCGAGCACCATAAAGAAAGCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGAcc                                                                                                                                      <  2:395330/140‑1 (MQ=255)
      aaGCGAGCACCATAAAGAAAGCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGcc                                                                                                                                  >  1:2038910/1‑140 (MQ=255)
      aaGCGAGCACCATAAAGAAAGCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGcc                                                                                                                                  >  1:2679521/1‑140 (MQ=255)
      aaGCGAGCACCATAAAGAAAGCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTGTGACATAGGAAAGACCTGcc                                                                                                                                  >  1:3821952/1‑140 (MQ=255)
      aaGCGAGCACCATAAAGAAAGCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCACAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGcc                                                                                                                                  >  1:62592/1‑140 (MQ=255)
          gAGCACCATAAAGAAAGCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGAc                                                                                                                                       <  1:3939019/131‑1 (MQ=255)
            gCACCATAAAGAAAGCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACCTTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATaa                                                                                                                            >  2:35155/1‑140 (MQ=255)
            gCACCATAAAGAAAGCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATaa                                                                                                                            >  2:238568/1‑140 (MQ=255)
            gCACCATAAAGAAAGCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATaa                                                                                                                            >  1:705922/1‑140 (MQ=255)
            gCACCATAAAGAAAGCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATaa                                                                                                                            >  2:2836733/1‑140 (MQ=255)
            gCACCATAAAGAAAGCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATaa                                                                                                                            >  1:3659445/1‑140 (MQ=255)
            gCACCATAAAGAAAGCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATaa                                                                                                                            >  2:3306264/1‑140 (MQ=255)
            gCACCATAAAGAAAGCGGGCAGATTGCCAGCCAAAAACCTCTCACCCAGCACATTAAGCGCAATAGTCACAGGGATGATTCACGCAGCCGTCGCCGAAAAATACGTCAGCGCTTTGACAATGGAAAGACCTGCCGCATaa                                                                                                                            >  1:1871908/1‑140 (MQ=255)
                  tAAAGAAAGCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAca                                                                                                                      >  2:1797399/1‑140 (MQ=255)
                      gaaagCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAcaaaat                                                                                                                  <  2:2582160/140‑1 (MQ=255)
                       aaagCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAcaaaatc                                                                                                                 >  1:153432/1‑140 (MQ=255)
                          gCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGAGATTGGAAAGACCTGCCGCATAACTGAcaaaatcaaa                                                                                                              >  2:1444193/1‑140 (MQ=255)
                          gCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGACAGACCTGCCGCATAACTGAcaaaatcaaa                                                                                                              >  2:3578370/1‑140 (MQ=255)
                          gCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCCTAACTGAcacaatctaa                                                                                                              >  2:2572438/1‑140 (MQ=255)
                          gCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAcaaaatcaaa                                                                                                              >  2:2938321/1‑140 (MQ=255)
                          gCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAcaaaatcaaa                                                                                                              >  2:500975/1‑140 (MQ=255)
                          gCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAcaaaatcaaa                                                                                                              >  2:4217863/1‑140 (MQ=255)
                          gCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAcaaaatcaaa                                                                                                              >  1:3639534/1‑140 (MQ=255)
                          gCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAcaaaatcaaa                                                                                                              >  2:3149111/1‑140 (MQ=255)
                          gCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAcaaaatcaaa                                                                                                              >  2:1335752/1‑140 (MQ=255)
                          gCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAcaaaatcaaa                                                                                                              >  2:3405034/1‑140 (MQ=255)
                          gCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAcaaaatcaaa                                                                                                              >  2:3480956/1‑140 (MQ=255)
                          gCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAcaaaatcaaa                                                                                                              >  2:2033209/1‑140 (MQ=255)
                          gCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAcaaaatcaaa                                                                                                              >  2:3558640/1‑140 (MQ=255)
                            gggCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAcaaaatcaaaat                                                                                                            >  1:1119614/1‑140 (MQ=255)
                                aGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGAt                                                                                                        <  2:2535085/140‑1 (MQ=255)
                                          ccaAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGAtt                                                                                              >  1:2655689/1‑140 (MQ=255)
                                                    cGCACCCAGCACGTTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAaaagaa                                                                                    <  1:2924906/140‑1 (MQ=255)
                                                    cGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTaaacgta                                                                                    >  1:3737094/1‑136 (MQ=255)
                                                    agCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAAGACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAaaagaa                                                                                    <  2:2156695/139‑1 (MQ=255)
                                                        cccATCACATGAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGAAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGat                                                                                <  2:3645433/140‑1 (MQ=255)
                                                        cccAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGat                                                                                <  2:2697752/140‑1 (MQ=255)
                                                        cacAGCACATTAAGCGCAATAGTCCCAGGGATGATTCACGCAGCCGTAGCCGCAAAATACGTCAGCGCTTTTACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGat                                                                                <  2:2067052/138‑1 (MQ=255)
                                                           aGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGAtata                                                                             >  1:665722/1‑140 (MQ=255)
                                                           aGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGAtata                                                                             >  2:3697378/1‑140 (MQ=255)
                                                            gCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTCAAAGAAAGATATaa                                                                            >  1:1272672/1‑140 (MQ=255)
                                                            gCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATaa                                                                            >  1:1987200/1‑140 (MQ=255)
                                                                 ttAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATCTaaaaaaa                                                                       >  2:45131/1‑140 (MQ=255)
                                                                 ttAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATaaaaaaa                                                                       >  2:2889983/1‑140 (MQ=255)
                                                                 ttAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATaaaaaaa                                                                       >  2:1438824/1‑140 (MQ=255)
                                                                 ttAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATaaaaaaa                                                                       >  2:1578316/1‑140 (MQ=255)
                                                                  ttaGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCTCATCAAAATAAGTCAGCGCTTTGACATTGGAAATACCTGCCGCATAACTGACAAAATCAAAATTGATCGGAAGGATTCTTAAAAGAAAGATATAAAAAAAt                                                                      <  1:1972847/138‑1 (MQ=255)
                                                                   aaGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATc                                                                     >  1:3901646/1‑140 (MQ=255)
                                                                   aaGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATc                                                                     >  1:3470950/1‑140 (MQ=255)
                                                                      cgcAAGAGTCCCAGGTATGATTCCCTCAGACGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTTACAAAATCAAAATTGATCGGCAGGTTTCTTAAAAGAAAGATATAAAAAAATCCGt                                                                  <  2:898683/140‑1 (MQ=255)
                                                                        cAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTa                                                                <  2:665722/140‑1 (MQ=255)
                                                                          atcgTCCCAGGGATGATTCCCACAGCCGTTGCGGCAAAATACGGCAGCGCTTTGACATTGGAAAGGCCTGCCGCATAGCTGACGAAATCAAAATTGATCGGCAGGATTCTCAAAAGAAAGATATAGAAAAATCCGTTAtc                                                              >  1:2640036/4‑140 (MQ=255)
                                                                          atcgTCCCAGGGATGATTCCCACAGCCGTTGCGGCAAAATACGGCAGCGCTTTGACATTGGAAAGGCCTGCCGCATAGCTGACGAAATCAAAATTGATCGGCAGGATTCTCAAAAGAAAGATATAGAAAAATCCGTTAtc                                                              >  1:2346410/4‑140 (MQ=255)
                                                                          aTAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTAtc                                                              >  1:2979427/1‑140 (MQ=255)
                                                                                ccAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCAt                                                        >  1:1147946/1‑140 (MQ=255)
                                                                                ccAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCAt                                                        <  1:1243874/140‑1 (MQ=255)
                                                                                 cAGGGATGATTCCCGCAGCCGTCGCCTCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATc                                                       <  2:2243000/140‑1 (MQ=255)
                                                                                 cAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATc                                                       <  1:2836733/140‑1 (MQ=255)
                                                                                 cAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATc                                                       <  1:2287952/140‑1 (MQ=255)
                                                                                 cAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATc                                                       <  2:3901646/140‑1 (MQ=255)
                                                                                 cAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATc                                                       <  2:4041803/140‑1 (MQ=255)
                                                                                   gggATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTg                                                     <  2:529441/140‑1 (MQ=255)
                                                                                   gggATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTg                                                     >  1:1656217/1‑140 (MQ=255)
                                                                                   gggATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTg                                                     >  1:1614952/1‑140 (MQ=255)
                                                                                   gggATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTg                                                     >  2:3099812/1‑140 (MQ=255)
                                                                                       tgatTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTc                                                 >  2:3019795/1‑140 (MQ=255)
                                                                                       tgatTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTc                                                 >  2:3019703/1‑140 (MQ=255)
                                                                                       tgatTCCCGCAGCCGTCACCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTc                                                 >  2:2342439/1‑140 (MQ=255)
                                                                                        gatTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAaaagaa                                                                                    >  2:116267/1‑104 (MQ=255)
                                                                                        gatTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTg                                                     >  2:2223838/1‑135 (MQ=255)
                                                                                        gatTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTg                                                     <  1:2223838/135‑1 (MQ=255)
                                                                                        gatTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCt                                                >  1:1161842/1‑140 (MQ=255)
                                                                                        gatTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCt                                                >  1:4331471/1‑140 (MQ=255)
                                                                                        gatTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCt                                                >  2:717128/1‑140 (MQ=255)
                                                                                         atTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATc                                                       <  2:3188992/132‑1 (MQ=255)
                                                                                         atTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTg                                               >  1:2768525/1‑140 (MQ=255)
                                                                                         atTCCCACAGCCGTTGCGGCAAAATACGGCAGCGCTTTGACATTGGAAAGGCCTGCCGCATAGCTGACGAAATCAAAATTGATCGGCAGGATTCTCAAAAGAAAGATATAGAAAAATCCGTTATCCTCCATTTGCTTCTg                                               >  2:171300/1‑140 (MQ=255)
                                                                                         atTCCCACAGCCGTTGCGGCAAAATACGGCAGCGCTTTGACATTGGAAAGGCCTGCCGCATAGCTGACGAAATCAAAATTGATCGGCAGGATTCTCAAAAGAAAGATATAGAAAAATCCGTTATCCTCCATTTGCTTCTg                                               >  2:178380/1‑140 (MQ=255)
                                                                                           tCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGaa                                             <  1:890001/140‑1 (MQ=255)
                                                                                           tCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGaa                                             <  1:2787443/140‑1 (MQ=255)
                                                                                           tCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGaa                                             <  1:3253608/140‑1 (MQ=255)
                                                                                           tCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGaa                                             <  1:563041/140‑1 (MQ=255)
                                                                                           tCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGaa                                             <  1:1444121/140‑1 (MQ=255)
                                                                                                cAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTCAAAGACAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGACTGGCt                                        >  2:372769/1‑140 (MQ=255)
                                                                                                     gTCGCCGCAACATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAg                                   <  1:2485877/140‑1 (MQ=255)
                                                                                                     gTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAg                                   <  1:3379768/140‑1 (MQ=255)
                                                                                                                    gTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGcc                    >  1:4187020/1‑140 (MQ=255)
                                                                                                                       aGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTa                                                                >  1:477978/1‑93 (MQ=255)
                                                                                                                       aGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGtt                 >  2:2789148/1‑140 (MQ=255)
                                                                                                                         cgcTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCt               >  1:1942079/1‑140 (MQ=255)
                                                                                                                           cTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCttt             >  1:3245883/1‑140 (MQ=255)
                                                                                                                                    tGGAAAGGCCTGCCGCATAGCTGACGAAATCAAAATTGATCGGCAGGATTCTCAAAAGAAAGATATAGAAAAATCCGTTATCCTCCATTTGCTTCTGTATGGCTTCAAGCCTTTCGTAATGCCCGTTCTTTTTCgccgaa    <  2:2640036/140‑5 (MQ=255)
                                                                                                                                    tGGAAAGGCCTGCCGCATAGCTGACGAAATCAAAATTGATCGGCAGGATTCTCAAAAGAAAGATATAGAAAAATCCGTTATCCTCCATTTGCTTCTGTATGGCTTCAAGCCTTTCGTAATGCCCGTTCTTTTTCgccgaa    <  2:908238/140‑5 (MQ=255)
                                                                                                                                    tGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCTGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCTTAATGGCCGTTCTTTTTCgaagca    >  1:2315587/1‑140 (MQ=255)
                                                                                                                                    tGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGACAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTCATGGCCGTTCTTTTTCgcagca    >  1:4030663/1‑140 (MQ=255)
                                                                                                                                    tGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATCTAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCgcagca    <  1:3098843/140‑1 (MQ=255)
                                                                                                                                    tGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTGCGTAATGGCCGTTCTTTTTCgcagca    >  2:3437828/1‑140 (MQ=255)
                                                                                                                                      gAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGcaaa  >  2:2412986/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                      
TATACAAAGCGAGCACCATAAAGAAAGCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAA  >  NZ_CP034943/2775713‑2775986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: