Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,775,864 A→G 5.6% S141S (AGT→AGC EO946_RS14350 ← TVP38/TMEM64 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,775,8640AG 5.6% 592.7 / 16.7 178S141S (AGT→AGCEO946_RS14350TVP38/TMEM64 family protein
Reads supporting (aligned to +/- strand):  ref base A (117/51);  new base G (6/4);  total (123/55)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 191 total aligned reads displayed.

GCACCATAAAGAAAGCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAA  >  NZ_CP034943/2775725‑2776002
                                                                                                                                           |                                                                                                                                          
gCACCATAAAGAAAGCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATaa                                                                                                                                            >  2:2836733/1‑140 (MQ=255)
gCACCATAAAGAAAGCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATaa                                                                                                                                            >  2:3451059/1‑140 (MQ=255)
gCACCATAAAGAAAGCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATaa                                                                                                                                            >  1:3494958/1‑140 (MQ=255)
gCACCATAAAGAAAGCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATaa                                                                                                                                            >  1:3659445/1‑140 (MQ=255)
gCACCATAAAGAAAGCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATaa                                                                                                                                            >  2:238568/1‑140 (MQ=255)
gCACCATAAAGAAAGCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATaa                                                                                                                                            >  1:1878353/1‑140 (MQ=255)
gCACCATAAAGAAAGCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATaa                                                                                                                                            >  1:3969433/1‑140 (MQ=255)
gCACCATAAAGAAAGCGGGCAGATTGCCAGCCAAAAAGCACGCACCCAGCACATTAAGCGCAATAGACCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATaa                                                                                                                                            >  2:4234463/1‑140 (MQ=255)
      tAAAGAAAGCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAca                                                                                                                                      >  2:1797399/1‑140 (MQ=255)
          gaaagCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAcaaaat                                                                                                                                  <  2:2582160/140‑1 (MQ=255)
           aaagCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAcaaaatc                                                                                                                                 >  1:153432/1‑140 (MQ=255)
              gCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGAGATTGGAAAGACCTGCCGCATAACTGAcaaaatcaaa                                                                                                                              >  2:1444193/1‑140 (MQ=255)
              gCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCCTAACTGAcacaatctaa                                                                                                                              >  2:2572438/1‑140 (MQ=255)
              gCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAccaaatcaaa                                                                                                                              >  2:398158/1‑140 (MQ=255)
              gCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAcaaaatcaaa                                                                                                                              >  2:3149111/1‑140 (MQ=255)
              gCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAcaaaatcaaa                                                                                                                              >  2:3428743/1‑140 (MQ=255)
              gCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAcaaaatcaaa                                                                                                                              >  2:2938321/1‑140 (MQ=255)
              gCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAcaaaatcaaa                                                                                                                              >  2:3527689/1‑140 (MQ=255)
              gCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAcaaaatcaaa                                                                                                                              >  2:3581506/1‑140 (MQ=255)
              gCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAcaaaatcaaa                                                                                                                              >  2:500975/1‑140 (MQ=255)
              gCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAcaaaatcaaa                                                                                                                              >  1:4041803/1‑140 (MQ=255)
              gCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAcaaaatcaaa                                                                                                                              >  2:1335752/1‑140 (MQ=255)
                gggCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAcaaaatcaaaat                                                                                                                            >  1:1119614/1‑140 (MQ=255)
                    aGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGAt                                                                                                                        <  2:2535085/140‑1 (MQ=255)
                        tGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGc                                                                                                                    >  1:2472510/1‑140 (MQ=255)
                              ccaAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGAtt                                                                                                              >  1:2655689/1‑140 (MQ=255)
                                 aaaaGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCtt                                                                                                           <  1:2313432/140‑1 (MQ=255)
                                        cGCACCCAGCACGTTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAaaagaa                                                                                                    <  1:2924906/140‑1 (MQ=255)
                                        cGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAaaagaa                                                                                                    <  1:82726/140‑1 (MQ=255)
                                        agCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAAGACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAaaagaa                                                                                                    <  2:2156695/139‑1 (MQ=255)
                                            cccAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGat                                                                                                <  2:2697752/140‑1 (MQ=255)
                                            cacAGCACATTAAGCGCAATAGTCCCAGGGATGATTCACGCAGCCGTAGCCGCAAAATACGTCAGCGCTTTTACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGat                                                                                                <  2:2067052/138‑1 (MQ=255)
                                               aGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGAtata                                                                                             >  1:665722/1‑140 (MQ=255)
                                               aGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGAtata                                                                                             >  2:365873/1‑140 (MQ=255)
                                                gCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTCAAAGAAAGATATaa                                                                                            >  1:1272672/1‑140 (MQ=255)
                                                gCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATaa                                                                                            >  1:1987200/1‑140 (MQ=255)
                                                     ttAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATaaaaaaa                                                                                       >  2:1438824/1‑140 (MQ=255)
                                                     ttAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATaaaaaaa                                                                                       >  2:2889983/1‑140 (MQ=255)
                                                     ttAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATaaaaaaa                                                                                       >  2:1578316/1‑140 (MQ=255)
                                                      ttaGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCTCATCAAAATAAGTCAGCGCTTTGACATTGGAAATACCTGCCGCATAACTGACAAAATCAAAATTGATCGGAAGGATTCTTAAAAGAAAGATATAAAAAAAt                                                                                      <  1:1972847/138‑1 (MQ=255)
                                                      tAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAAt                                                                                      <  1:266116/140‑1 (MQ=255)
                                                       aaGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATc                                                                                     >  1:3901646/1‑140 (MQ=255)
                                                       aaGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATc                                                                                     >  1:3470950/1‑140 (MQ=255)
                                                         gcgcAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATcag                                                                                   >  2:3577491/1‑138 (MQ=255)
                                                          cgcAAGAGTCCCAGGTATGATTCCCTCAGACGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTTACAAAATCAAAATTGATCGGCAGGTTTCTTAAAAGAAAGATATAAAAAAATCCGt                                                                                  <  2:898683/140‑1 (MQ=255)
                                                            cAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTa                                                                                <  2:665722/140‑1 (MQ=255)
                                                              atcgTCCCAGGGATGATTCCCACAGCCGTTGCGGCAAAATACGGCAGCGCTTTGACATTGGAAAGGCCTGCCGCATAGCTGACGAAATCAAAATTGATCGGCAGGATTCTCAAAAGAAAGATATAGAAAAATCCGTTAtc                                                                              >  1:908238/4‑140 (MQ=255)
                                                              atcgTCCCAGGGATGATTCCCACAGCCGTTGCGGCAAAATACGGCAGCGCTTTGACATTGGAAAGGCCTGCCGCATAGCTGACGAAATCAAAATTGATCGGCAGGATTCTCAAAAGAAAGATATAGAAAAATCCGTTAtc                                                                              >  1:2640036/4‑140 (MQ=255)
                                                              aTAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTAtc                                                                              >  1:2979427/1‑140 (MQ=255)
                                                                    ccAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCAt                                                                        >  1:1147946/1‑140 (MQ=255)
                                                                    ccAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCAt                                                                        >  1:2777818/1‑140 (MQ=255)
                                                                    ccAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCAt                                                                        <  1:3775789/140‑1 (MQ=255)
                                                                    ccAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCAt                                                                        <  1:1243874/140‑1 (MQ=255)
                                                                     cAGGGATGATTCCCGCAGCCGTCGCCTCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATc                                                                       <  2:2243000/140‑1 (MQ=255)
                                                                       gggATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTg                                                                     >  2:3379768/1‑140 (MQ=255)
                                                                       gggATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTg                                                                     >  2:3099812/1‑140 (MQ=255)
                                                                       gggATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTg                                                                     >  1:2342370/1‑140 (MQ=255)
                                                                       gggATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTg                                                                     >  1:1656217/1‑140 (MQ=255)
                                                                       gggATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTg                                                                     >  1:1614952/1‑140 (MQ=255)
                                                                           tgatTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTc                                                                 >  2:3019795/1‑140 (MQ=255)
                                                                           tgatTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTc                                                                 >  2:3019703/1‑140 (MQ=255)
                                                                           tgatTCCCGCAGCCGTCACCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTc                                                                 >  2:2342439/1‑140 (MQ=255)
                                                                            gatTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAaaagaa                                                                                                    >  2:116267/1‑104 (MQ=255)
                                                                            gatTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTg                                                                     >  2:2223838/1‑135 (MQ=255)
                                                                            gatTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCt                                                                >  1:2244470/1‑140 (MQ=255)
                                                                            gatTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCt                                                                >  1:695720/1‑140 (MQ=255)
                                                                            gatTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCt                                                                >  2:717128/1‑140 (MQ=255)
                                                                            gatTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCt                                                                >  1:1161842/1‑140 (MQ=255)
                                                                             atTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATc                                                                       <  2:3188992/132‑1 (MQ=255)
                                                                             atTCCCACAGCCGTTGCGGCAAAATACGGCAGCGCTTTGACATTGGAAAGGCCTGCCGCATAGCTGACGAAATCAAAATTGATCGGCAGGATTCTCAAAAGAAAGATATAGAAAAATCCGTTATCCTCCATTTGCTTCTg                                                               >  2:178380/1‑140 (MQ=255)
                                                                             atTCCCACAGCCGTTGCGGCAAAATACGGCAGCGCTTTGACATTGGAAAGGCCTGCCGCATAGCTGACGAAATCAAAATTGATCGGCAGGATTCTCAAAAGAAAGATATAGAAAAATCCGTTATCCTCCATTTGCTTCTg                                                               >  2:171300/1‑140 (MQ=255)
                                                                               tCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGaa                                                             <  1:563041/140‑1 (MQ=255)
                                                                               tCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGaa                                                             <  1:3253608/140‑1 (MQ=255)
                                                                               tCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGaa                                                             <  1:1444121/140‑1 (MQ=255)
                                                                                cccGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAAt                                                            <  2:3489722/140‑1 (MQ=255)
                                                                                    cAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTCAAAGACAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGACTGGCt                                                        >  2:372769/1‑140 (MQ=255)
                                                                                    cAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCt                                                        >  2:372709/1‑140 (MQ=255)
                                                                                         gTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAg                                                   <  1:3379768/140‑1 (MQ=255)
                                                                                                    aTACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAAt                                        <  2:4078791/140‑1 (MQ=255)
                                                                                                           aGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTa                                                                                >  1:477978/1‑93 (MQ=255)
                                                                                                           aGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGtt                                 >  2:2789148/1‑140 (MQ=255)
                                                                                                               cTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCttt                             >  1:3245883/1‑140 (MQ=255)
                                                                                                                        tGGAAAGGCCTGCCGCATAGCTGACGAAATCAAAATTGATCGGCAGGATTCTCAAAAGAAAGATATAGAAAAATCCGTTATCCTCCATTTGCTTCTGTATGGCTTCAAGCCTTTCGTAATGCCCGTTCTTTTTCgccgaa                    <  2:908238/140‑5 (MQ=255)
                                                                                                                        tGGAAAGGCCTGCCGCATAGCTGACGAAATCAAAATTGATCGGCAGGATTCTCAAAAGAAAGATATAGAAAAATCCGTTATCCTCCATTTGCTTCTGTATGGCTTCAAGCCTTTCGTAATGCCCGTTCTTTTTCgccgaa                    <  2:2640036/140‑5 (MQ=255)
                                                                                                                        tGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATCTAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCgcagca                    <  1:3098843/140‑1 (MQ=255)
                                                                                                                          gAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGcaaa                  >  2:2412986/1‑140 (MQ=255)
                                                                                                                                  tGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAg          >  2:455340/1‑140 (MQ=255)
                                                                                                                                    ccGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGcc        >  1:2037011/1‑140 (MQ=255)
                                                                                                                                    ccGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGcc        >  1:1980977/1‑140 (MQ=255)
                                                                                                                                    ccGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGcc        >  1:2148004/1‑140 (MQ=255)
                                                                                                                                    ccGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGcc        >  2:1966708/1‑140 (MQ=255)
                                                                                                                                    ccGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGcc        >  1:4291944/1‑140 (MQ=255)
                                                                                                                                    ccGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGcc        >  1:4212818/1‑140 (MQ=255)
                                                                                                                                    ccGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGcc        >  1:2501561/1‑140 (MQ=255)
                                                                                                                                    ccGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGcc        >  1:1401852/1‑140 (MQ=255)
                                                                                                                                    ccGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGcc        >  2:572366/1‑140 (MQ=255)
                                                                                                                                    ccGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGcc        >  2:1669179/1‑140 (MQ=255)
                                                                                                                                    ccGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGcc        >  1:281535/1‑140 (MQ=255)
                                                                                                                                    ccGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGcc        >  1:2838424/1‑140 (MQ=255)
                                                                                                                                          aaCTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCaaaa  <  2:3245883/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
GCACCATAAAGAAAGCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAA  >  NZ_CP034943/2775725‑2776002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: