Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 438,792 C→A 8.7% intergenic (‑58/‑48) gabR ← / → gabT transcriptional regulator GabR/4‑aminobutyrate‑‑2‑oxoglutarate transaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943438,7920CA 8.7% 511.4 / 6.0 217intergenic (‑58/‑48)gabR/gabTtranscriptional regulator GabR/4‑aminobutyrate‑‑2‑oxoglutarate transaminase
Reads supporting (aligned to +/- strand):  ref base C (117/73);  new base A (4/23);  total (121/96)
Fisher's exact test for biased strand distribution p-value = 4.86e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 221 total aligned reads displayed.

TTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAACGGCAACCGCAG  >  NZ_CP034943/438658‑438931
                                                                                                                                      |                                                                                                                                           
ttttttTGTTTTTTCTAAATTTGCTGATAGATATAATCGTCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTACATTTGCAGATTCTCCTTCTGGTACCATAAAAAAGTTATAATTGGTACTTTTCATCATACCaaaaa                                                                                                                                        <  1:3668990/140‑1 (MQ=255)
ttttttAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCaaaaa                                                                                                                                        <  1:3171199/140‑1 (MQ=255)
ttttttAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCaaaaa                                                                                                                                        <  1:111402/140‑1 (MQ=255)
         ttttGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGATCGGTCGATTTTGAATGTGATGTCCATTTGCAGATTCTCCGTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGa                                                                                                                               <  2:1229935/140‑1 (MQ=255)
         ttttGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCAGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGa                                                                                                                               <  2:3040047/140‑1 (MQ=255)
              aTAACTTTGCTGATAGATCTAATCGGCTTGTTCTGAGCGGTCGAGTGAGAATGTGATGTCCAATTGCAGATTCTCCATCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGt                                                                                                                          <  1:2838442/140‑1 (MQ=255)
              aTAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGt                                                                                                                          <  1:1544316/140‑1 (MQ=255)
              aTAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGt                                                                                                                          <  2:2136752/140‑1 (MQ=255)
                    ttGCTGATAGATATAATCGGATTTTTCTGAGCGGTCGAGTTTGATTTTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAATTTATAATTGGTACTTTTCATCATACCAAAAATAAGTCAGAATGGTAATaaa                                                                                                                    <  1:1484052/140‑1 (MQ=255)
                              ctataCTCGGCTTGTACTGAGCCGTCGAGTGTGAATGTGATGTCAATTTGCTGATTATACTTCTGTTACCATCAAAAAGTTTTTATTTGTACTTTTCATTATACCAAAATGTTGTCAGATTGTTATGATAATAGCGACAg                                                                                                          <  2:2179854/139‑1 (MQ=255)
                              atttaATCGGCTTGTTCTGAGGGGTGGAGTGTGAATGTGATGTCCTTTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGTTAAGAAAATAGCGACAg                                                                                                          <  2:2407572/137‑1 (MQ=255)
                                ataATCGGCTTGTTCTGAGCGGTCTAGTGTTATTGTGATGTCCATTTGCAGATTCTCCTTCTGGTACAATCAAAAAGTTATAATTGTTACTTTTAATCATACCAAAAAGAAGTCAGAATGTTAAGAAAATAGCGACAggg                                                                                                        <  2:785137/140‑1 (MQ=255)
                                  aaTGGGCTTTTTCTGAGCGGTCGAGTGTGAATTTGATGTCCATTTGCAGTTTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAggggg                                                                                                      <  2:2360490/140‑1 (MQ=255)
                                    tCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGAc                                                                                                    >  1:3264922/1‑140 (MQ=255)
                                     gggCTTTTTCTGAGCGGTCGTGTGTTAATGTGATTTCATTTTGCATATTCTCCTTCTGTTACCATCAAAAATTTATAATTGGTACTTTTCATCATACAAAAAAGAAGTCATAATGGTAAGAAAATAGCGACAGGGGGACa                                                                                                   <  2:535168/139‑1 (MQ=255)
                                     gggCTGGTGCTGCGCGGTCGAGTGTGAATGTGATGGCCATTTGCACATTCTCCTTCGGGTACCATCAAAAAGTTATAATTGGAACTTTTCATCATACCAAAAAGAAGTCACAATGGTAAGAAAATAGCGACAGGGGGACa                                                                                                   <  2:2224768/139‑1 (MQ=255)
                                       gCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATa                                                                                                 >  2:2870061/1‑140 (MQ=255)
                                        cTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATAc                                                                                                >  2:4158617/1‑140 (MQ=255)
                                         ttGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACg                                                                                               >  1:3829512/1‑140 (MQ=255)
                                         ttGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACg                                                                                               >  1:1079759/1‑140 (MQ=255)
                                          tGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGc                                                                                              >  2:3328748/1‑140 (MQ=255)
                                          tGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGc                                                                                              >  2:1041034/1‑140 (MQ=255)
                                          tGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTACATCATACCAAAAAGAAGTCAGAATGTTAAGAAAATAGAGACAGGGGCACATACGc                                                                                              >  2:2302801/1‑140 (MQ=255)
                                          tGTTCGGAGGGGTCTTTTGTGAATGTGATGTCCATTTGCAGATTCTCCTACTGGTACCAACAAAAATTTATAATTGGTACTTTTCATCATACAAAAAATAAGACAGAATGGTAACAAAATAGCGACAGGGGGACATACGc                                                                                              <  1:3936178/140‑1 (MQ=255)
                                           gTTGTGGGCGGACGTGTGTCAATGTGTTGTCAAGTTGCAGATTCTCCTTCTGGTACCATCAAAATGTTATAATTGGTAGTTTTCAACATACAAAAAATAAGTCAGAATTGTATGAAAATAGCGACAGGGGGACATACGCa                                                                                             <  2:2713692/140‑1 (MQ=255)
                                           gTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCa                                                                                             <  2:910582/140‑1 (MQ=255)
                                            ttCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCAt                                                                                            >  2:3130064/1‑140 (MQ=255)
                                            ttCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCAt                                                                                            >  2:1163181/1‑140 (MQ=255)
                                            ttCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCAt                                                                                            >  2:508576/1‑140 (MQ=255)
                                                 aGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACATGGGGACATACGCATGAGTc                                                                                       >  1:1273344/1‑140 (MQ=255)
                                                 aGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTc                                                                                       >  1:1773672/1‑140 (MQ=255)
                                                 aGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTc                                                                                       >  1:1011961/1‑140 (MQ=255)
                                                 aGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTc                                                                                       >  2:1181967/1‑140 (MQ=255)
                                                 aGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTc                                                                                       >  2:4191876/1‑140 (MQ=255)
                                                 aGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTc                                                                                       >  1:3296546/1‑140 (MQ=255)
                                                 aGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTc                                                                                       >  2:3694727/1‑140 (MQ=255)
                                                    gggcGAGTGTGAATGTTATTTCCATTGGCACATTCACCTTCTGGAACCATCAAAAAGTTTTACTTGCTACTTTTCATAATACAAAAAATAAGTCAGAAGGGTATGAAAAAAGCGACAGGGGGACATACGCATGAGTCaaa                                                                                    <  2:3034362/137‑1 (MQ=255)
                                                     gTCGAGTTTGAATGTTATGTCCATTTGCATATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACAAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAaac                                                                                   <  2:3498343/140‑1 (MQ=255)
                                                     gTCGAGTGTTAATGTTATGTACTTTTGCATATTCTCCTTCGGGTACAATAAAAAAGTTATATTTGGTACTTTACATAATACAAAAAATAAGAAAGATTGTTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAaac                                                                                   <  1:2981311/140‑1 (MQ=255)
                                                     gTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAaac                                                                                   >  1:500272/1‑140 (MQ=255)
                                                      tCGAGTGTGACTGTGATGTCCATGTGCAGATTCTCCTTCTGGTACCAGCAAAAAGTGATCATTGGTACTTTTCATCATACCAAAAAGAAGTCCGAATGGTCAGAAAATAGCGACAGGGGGACATACGCATGAGTCAaaca                                                                                  >  1:2061368/1‑140 (MQ=255)
                                                      tCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGACTGGTAAGAAAATAGCGACAGGGGGACATAAGCATGAGTCAaaca                                                                                  >  2:830332/1‑140 (MQ=255)
                                                      tCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAaaca                                                                                  >  2:3908268/1‑140 (MQ=255)
                                                      tCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAaaca                                                                                  >  2:977631/1‑140 (MQ=255)
                                                      tCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAaaca                                                                                  >  2:1696819/1‑140 (MQ=255)
                                                      tCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAaaca                                                                                  >  2:166386/1‑140 (MQ=255)
                                                      tCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAaaca                                                                                  >  2:1497213/1‑140 (MQ=255)
                                                      tCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAaaca                                                                                  >  1:3540420/1‑140 (MQ=255)
                                                      tCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAaaca                                                                                  >  1:3607833/1‑140 (MQ=255)
                                                      tCGAGTGTGAATGTGATGTCCATTTGCAGATTCTACTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACAAAAAAGAATTCAGAATGGTAAGAAAATAGCGACAGGGGGACATAAGAATGAGACAaaca                                                                                  >  2:703113/1‑140 (MQ=255)
                                                      aacattgtcAATGTGATGTCAATAAGAAAAATCTACTTATCGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAaaca                                                                                  >  2:1912640/6‑140 (MQ=255)
                                                            ttcattgtgATGACCATTTCACTTTTCTCCTTCTGGTACCAAAAAAAATTTATAATTGGAACTTTAAATCATACCAAAAATAAGACAGATTGTTAACAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACagca                                                                            <  1:2214942/135‑1 (MQ=255)
                                                            gttaaTTTGATGTCCTTTTGCTTTTTCTACTTCTGTTAACATAAAAAATTTATAATTGGTACTTTTCATCATACCAAAAATAAGTCAGAATGGTAATAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACagca                                                                            <  2:2157142/137‑1 (MQ=255)
                                                            gtgATTGTGATGGCCTTTTTCTTATTCTCCTTGTGGTACCATAAAAAATTTTTATTTGGTACTTTTAATCATACAAAAAATAAGTAAGATTGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACagca                                                                            <  1:3490288/140‑1 (MQ=255)
                                                            gtgAATGTGATGTCCATTTGCATATTCTCCTTCTGGTACCATAAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACagca                                                                            <  2:3957823/140‑1 (MQ=255)
                                                                aTGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCACAAAGAAGTCAGAATGCTAAGAAAATAGCGACAGGGGGACATACGCAGGAGTCAAACAACagcaagca                                                                        >  2:2485634/1‑140 (MQ=255)
                                                                aTGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATATCGACAGGGGGACATACGCATGAGTCAAACAACagcaagca                                                                        >  2:2840498/1‑140 (MQ=255)
                                                                aTGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACagcaagca                                                                        >  1:2769776/1‑140 (MQ=255)
                                                                aTGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACAAAAAAGAAGTCAGAATGCTAAGAAAATAGCGACAGGGGGACATACGCAGGAGTCAAACAACagcaagca                                                                        >  2:1472723/1‑140 (MQ=255)
                                                                 ttttaTGTCCTTTTCCATATTCTCCTTCTGTTACCATAAAAATGTTATAATTGTTAATTTTCAACATACAAAAAATAACTCATATTGGTAATAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCAt                                                                       <  1:977631/136‑1 (MQ=255)
                                                                 tgttATGTCCATGTGAATATTCTCCTTCTGGTACAATAAAAAATTTATAATTGTTACTTTTAATCATACCAAAAAGAAGTCAGAATGTTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCAt                                                                       <  2:3409469/140‑1 (MQ=255)
                                                                 tgtgATGTCCATTTGCAGATTCTCCTTCTGGTACCATAAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCAt                                                                       <  2:4037375/140‑1 (MQ=255)
                                                                    gATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCAc                                                                    >  2:4279985/1‑140 (MQ=255)
                                                                     aTGTCCATTTGCAGATTCTCCTTCCGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAATTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACg                                                                   >  2:2901731/1‑140 (MQ=255)
                                                                     aTGTCCATTTGCAGATTATCATTCTGGTACCATCAAAAAGATATAATAGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGAGACATGGCCACATACGCATGAGTCAAACAAAAGAAAGCATCAct                                                                   >  2:867563/1‑139 (MQ=255)
                                                                       gTCCTTTTGCAGATACTCCTACTGGTACAATCAAAAATTTATAATTGGTACTTTTCATCATACAAAAAACAAGTCACAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGAc                                                                 <  1:408680/140‑1 (MQ=255)
                                                                       gTCCATTTGCAGATACTACTTCTGGAACCAACAAAAAGTTATAATTGTTACTTTACATCATACAAAAAAGAAGTAAGAATGCTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGAc                                                                 <  1:4222113/140‑1 (MQ=255)
                                                                            tttGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGccc                                                            >  2:2393261/1‑140 (MQ=255)
                                                                                cAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTg                                                        >  2:1441406/1‑140 (MQ=255)
                                                                                     tctcCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGgcagc                                                   >  2:2323665/1‑140 (MQ=255)
                                                                                       tcCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCaa                                                 >  1:2799116/1‑140 (MQ=255)
                                                                                              ggTACCATAAAATAGTTATTTTTGTTATTTTAAATCATACCAAAAAGAAGTCAGATTGTAAATAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACggg                                          <  1:2609021/140‑1 (MQ=255)
                                                                                               gTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGa                                         >  1:1203076/1‑140 (MQ=255)
                                                                                               gTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGa                                         >  1:1202558/1‑140 (MQ=255)
                                                                                                 aCCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGc                                       >  2:612942/1‑140 (MQ=255)
                                                                                                 aCCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGc                                       >  2:2604512/1‑140 (MQ=255)
                                                                                                     tCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGTATGGGAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCACtt                                   >  2:2204633/1‑140 (MQ=255)
                                                                                                     tCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCCACACCGGGAGCCAtt                                   >  2:2985861/1‑140 (MQ=255)
                                                                                                     tCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAAtt                                   >  2:4088272/1‑140 (MQ=255)
                                                                                                     tCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAAtt                                   >  2:756445/1‑140 (MQ=255)
                                                                                                        aaaaGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGTTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGt                                <  1:2896068/140‑1 (MQ=255)
                                                                                                            gTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGACAATAGCGACATGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACCGGAGCAATACGTCTcc                            >  2:3613062/1‑140 (MQ=255)
                                                                                                             ttATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGTTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCa                           >  1:820385/1‑140 (MQ=255)
                                                                                                             ttATAATTGGTACTTTTCATCATACAAAAAAGAAGTCATAAGGGAAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCa                           <  1:2232556/140‑1 (MQ=255)
                                                                                                              tataATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCaa                          >  1:2178843/1‑140 (MQ=255)
                                                                                                               ataATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCaaa                         >  1:613662/1‑140 (MQ=255)
                                                                                                                  aTTGGTAGTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGgt                      <  2:3638166/140‑1 (MQ=255)
                                                                                                                      gTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGa                  >  1:3001923/1‑140 (MQ=255)
                                                                                                                       tACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAg                 >  2:2057214/1‑140 (MQ=255)
                                                                                                                         gttttCATCATACCAAAAATAAGCCAGATTGGTAATAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCa               <  1:166386/139‑1 (MQ=255)
                                                                                                                         cTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAATCGGGAGCAATTCGTCTCCAAAGGTGTGAGCa               <  2:3250241/140‑1 (MQ=255)
                                                                                                                         cTTTTCATCATACCAAAAACAAGTAAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCa               <  2:1601099/140‑1 (MQ=255)
                                                                                                                           tttCATCCTCCCGAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAAc             <  2:215574/140‑1 (MQ=255)
                                                                                                                           tttCATCATACCAAAAATAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAAc             <  1:2374204/140‑1 (MQ=255)
                                                                                                                              aatcatACCAAAAATAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAACGGc          <  2:1398677/139‑1 (MQ=255)
                                                                                                                               atcatACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAACGGCa         >  1:3129247/1‑140 (MQ=255)
                                                                                                                                   tACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAACGGCAACCg     <  2:1202628/140‑1 (MQ=255)
                                                                                                                                   tACCAAAAAGAAGTCACAAGGTTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAACGGCAACCg     <  1:428138/140‑1 (MQ=255)
                                                                                                                                    aCCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAATCAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAACGGCAACCGc    >  2:2702374/1‑140 (MQ=255)
                                                                                                                                      cAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAACGGCAACCGCAg  >  2:1687902/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                           
TTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAACGGCAACCGCAG  >  NZ_CP034943/438658‑438931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: