Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,775,870 A→G 6.3% F139F (TTT→TTC EO946_RS14350 ← TVP38/TMEM64 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,775,8700AG 6.3% 518.5 / 17.5 158F139F (TTT→TTCEO946_RS14350TVP38/TMEM64 family protein
Reads supporting (aligned to +/- strand):  ref base A (92/56);  new base G (6/4);  total (98/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

Only 100 of 189 total aligned reads displayed.

TAAAGAAAGCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAA  >  NZ_CP034943/2775731‑2776009
                                                                                                                                           |                                                                                                                                           
     aaagCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAcaaaatc                                                                                                                                        >  1:153432/1‑140 (MQ=255)
        gCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCTGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAcaaactcaaa                                                                                                                                     >  2:3208137/1‑140 (MQ=255)
        gCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGAGATTGGAAAGACCTGCCGCATAACTGAcaaaatcaaa                                                                                                                                     >  2:1444193/1‑140 (MQ=255)
        gCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGACAGACCTGCCGCATAACTGAcaaaatcaaa                                                                                                                                     >  2:3578370/1‑140 (MQ=255)
        gCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCCTAACTGAcacaatctaa                                                                                                                                     >  2:2572438/1‑140 (MQ=255)
        gCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAcaaaatcaaa                                                                                                                                     >  2:2938321/1‑140 (MQ=255)
        gCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAcaaaatcaaa                                                                                                                                     >  2:1335752/1‑140 (MQ=255)
        gCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAcaaaatcaaa                                                                                                                                     >  2:1677765/1‑140 (MQ=255)
        gCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAcaaaatcaaa                                                                                                                                     >  2:2033209/1‑140 (MQ=255)
        gCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAcaaaatcaaa                                                                                                                                     >  1:4258343/1‑140 (MQ=255)
        gCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAcaaaatcaaa                                                                                                                                     >  2:3149111/1‑140 (MQ=255)
        gCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAcaaaatcaaa                                                                                                                                     >  2:3558640/1‑140 (MQ=255)
        gCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAcaaaatcaaa                                                                                                                                     >  2:672577/1‑140 (MQ=255)
        gCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAcaaaatcaaa                                                                                                                                     >  2:3428743/1‑140 (MQ=255)
          gggCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAcaaaatcaaaat                                                                                                                                   >  1:1119614/1‑140 (MQ=255)
              aGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGAt                                                                                                                               <  2:2535085/140‑1 (MQ=255)
                  tGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGc                                                                                                                           >  1:2472510/1‑140 (MQ=255)
                        ccaAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGAtt                                                                                                                     >  1:583592/1‑140 (MQ=255)
                        ccaAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGAtt                                                                                                                     >  1:2655689/1‑140 (MQ=255)
                                  cGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTaaacgta                                                                                                           >  1:3737094/1‑136 (MQ=255)
                                  agCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAAGACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAaaagaa                                                                                                           <  2:2156695/139‑1 (MQ=255)
                                      cccATCACATGAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGAAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGat                                                                                                       <  2:3645433/140‑1 (MQ=255)
                                         aGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGAtata                                                                                                    >  2:3697378/1‑140 (MQ=255)
                                          gCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTCAAAGAAAGATATaa                                                                                                   >  1:1272672/1‑140 (MQ=255)
                                          gCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATaa                                                                                                   >  1:1987200/1‑140 (MQ=255)
                                               ttAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATCTaaaaaaa                                                                                              >  2:45131/1‑140 (MQ=255)
                                               ttAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATaaaaaaa                                                                                              >  2:2889983/1‑140 (MQ=255)
                                               ttAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATaaaaaaa                                                                                              >  2:1578316/1‑140 (MQ=255)
                                               ttAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATaaaaaaa                                                                                              >  2:1438824/1‑140 (MQ=255)
                                                tAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAAt                                                                                             <  2:1099837/140‑1 (MQ=255)
                                                 aaGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATc                                                                                            >  1:3901646/1‑140 (MQ=255)
                                                    cgcAAGAGTCCCAGGTATGATTCCCTCAGACGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTTACAAAATCAAAATTGATCGGCAGGTTTCTTAAAAGAAAGATATAAAAAAATCCGt                                                                                         <  2:898683/140‑1 (MQ=255)
                                                        atcgTCCCAGGGATGATTCCCACAGCCGTTGCGGCAAAATACGGCAGCGCTTTGACATTGGAAAGGCCTGCCGCATAGCTGACGAAATCAAAATTGATCGGCAGGATTCTCAAAAGAAAGATATAGAAAAATCCGTTAtc                                                                                     >  1:908238/4‑140 (MQ=255)
                                                        atcgTCCCAGGGATGATTCCCACAGCCGTTGCGGCAAAATACGGCAGCGCTTTGACATTGGAAAGGCCTGCCGCATAGCTGACGAAATCAAAATTGATCGGCAGGATTCTCAAAAGAAAGATATAGAAAAATCCGTTAtc                                                                                     >  1:2640036/4‑140 (MQ=255)
                                                        atcgTCCCAGGGATGATTCCCACAGCCGTTGCGGCAAAATACGGCAGCGCTTTGACATTGGAAAGGCCTGCCGCATAGCTGACGAAATCAAAATTGATCGGCAGGATTCTCAAAAGAAAGATATAGAAAAATCCGTTAtc                                                                                     >  1:2346410/4‑140 (MQ=255)
                                                        aTAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTAtc                                                                                     >  1:2979427/1‑140 (MQ=255)
                                                              ccAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCAt                                                                               >  1:1147946/1‑140 (MQ=255)
                                                              ccAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCAt                                                                               <  1:1243874/140‑1 (MQ=255)
                                                               cAGGGATGATTCCCGCAGCCGTCGCCTCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATc                                                                              <  2:2243000/140‑1 (MQ=255)
                                                               cAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATc                                                                              <  1:2287952/140‑1 (MQ=255)
                                                               cAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATc                                                                              <  2:3901646/140‑1 (MQ=255)
                                                               cAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATc                                                                              <  2:4041803/140‑1 (MQ=255)
                                                               cAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATc                                                                              <  1:2836733/140‑1 (MQ=255)
                                                               cAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTGATCCTCCATc                                                                              <  1:3451059/140‑1 (MQ=255)
                                                                 gggATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTg                                                                            >  1:3825516/1‑140 (MQ=255)
                                                                 gggATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTg                                                                            >  2:3379768/1‑140 (MQ=255)
                                                                 gggATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTg                                                                            >  2:3099812/1‑140 (MQ=255)
                                                                 gggATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTg                                                                            >  1:1656217/1‑140 (MQ=255)
                                                                 gggATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTg                                                                            >  1:1614952/1‑140 (MQ=255)
                                                                 gggATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTg                                                                            <  2:529441/140‑1 (MQ=255)
                                                                     tgatTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTc                                                                        >  2:3019795/1‑140 (MQ=255)
                                                                     tgatTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTc                                                                        >  2:3019703/1‑140 (MQ=255)
                                                                     tgatTCCCGCAGCCGTCACCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTc                                                                        >  2:2342439/1‑140 (MQ=255)
                                                                      gatTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTg                                                                            <  1:2223838/135‑1 (MQ=255)
                                                                      gatTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTg                                                                            >  2:2223838/1‑135 (MQ=255)
                                                                      gatTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCt                                                                       >  1:4331471/1‑140 (MQ=255)
                                                                      gatTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCt                                                                       >  1:695720/1‑140 (MQ=255)
                                                                      gatTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCt                                                                       >  1:1161842/1‑140 (MQ=255)
                                                                       atTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTg                                                                      >  1:2768525/1‑140 (MQ=255)
                                                                       atTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTg                                                                      >  2:2873725/1‑140 (MQ=255)
                                                                       atTCCCACAGCCGTTGCGGCAAAATACGGCAGCGCTTTGACATTGGAAAGGCCTGCCGCATAGCTGACGAAATCAAAATTGATCGGCAGGATTCTCAAAAGAAAGATATAGAAAAATCCGTTATCCTCCATTTGCTTCTg                                                                      >  2:178380/1‑140 (MQ=255)
                                                                       atTCCCACAGCCGTTGCGGCAAAATACGGCAGCGCTTTGACATTGGAAAGGCCTGCCGCATAGCTGACGAAATCAAAATTGATCGGCAGGATTCTCAAAAGAAAGATATAGAAAAATCCGTTATCCTCCATTTGCTTCTg                                                                      >  2:171300/1‑140 (MQ=255)
                                                                        ttCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGa                                                                     >  2:2606183/1‑140 (MQ=255)
                                                                         tctcGCAGCCGTAGCCGCAAAATACGTCAGAGCTTTTAAATTGGAAAGACCAGCCGAATAACTGACAAAATCAATATTGCTCGGCAGGATTATTAAAATAAAGATATAAAAAAATCCGTTATCCTCCATCTTCTTCTGaa                                                                    <  2:1871908/137‑1 (MQ=255)
                                                                         tCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGaa                                                                    <  1:2787443/140‑1 (MQ=255)
                                                                         tCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGaa                                                                    <  1:890001/140‑1 (MQ=255)
                                                                         tCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGaa                                                                    <  1:3253608/140‑1 (MQ=255)
                                                                         tCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGaa                                                                    <  1:1444121/140‑1 (MQ=255)
                                                                         tCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGaa                                                                    <  1:555713/140‑1 (MQ=255)
                                                                          cccGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAAt                                                                   <  2:3489722/140‑1 (MQ=255)
                                                                              cAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTCAAAGACAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGACTGGCt                                                               >  2:372769/1‑140 (MQ=255)
                                                                                   gTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAg                                                          <  1:3379768/140‑1 (MQ=255)
                                                                                              aTACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAAt                                               <  2:4078791/140‑1 (MQ=255)
                                                                                              aTACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAAt                                               <  2:64900/140‑1 (MQ=255)
                                                                                                  gTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGcc                                           >  1:4187020/1‑140 (MQ=255)
                                                                                                     aGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTa                                                                                       <  2:477978/93‑1 (MQ=255)
                                                                                                     aGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGtt                                        >  2:2789148/1‑140 (MQ=255)
                                                                                                       cgcTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCt                                      >  1:1942079/1‑140 (MQ=255)
                                                                                                         cTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCttt                                    >  1:3245883/1‑140 (MQ=255)
                                                                                                                  tGGAAAGGCCTGCCGCATAGCTGACGAAATCAAAATTGATCGGCAGGATTCTCAAAAGAAAGATATAGAAAAATCCGTTATCCTCCATTTGCTTCTGTATGGCTTCAAGCCTTTCGTAATGCCCGTTCTTTTTCgccgaa                           <  2:908238/140‑5 (MQ=255)
                                                                                                                  tGGAAAGGCCTGCCGCATAGCTGACGAAATCAAAATTGATCGGCAGGATTCTCAAAAGAAAGATATAGAAAAATCCGTTATCCTCCATTTGCTTCTGTATGGCTTCAAGCCTTTCGTAATGCCCGTTCTTTTTCgccgaa                           <  2:2640047/140‑5 (MQ=255)
                                                                                                                  tGGAAAGGCCTGCCGCATAGCTGACGAAATCAAAATTGATCGGCAGGATTCTCAAAAGAAAGATATAGAAAAATCCGTTATCCTCCATTTGCTTCTGTATGGCTTCAAGCCTTTCGTAATGCCCGTTCTTTTTCgccgaa                           <  2:2346410/140‑5 (MQ=255)
                                                                                                                  tGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCTGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCTTAATGGCCGTTCTTTTTCgaagca                           >  1:2315587/1‑140 (MQ=255)
                                                                                                                  tGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGACAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTCATGGCCGTTCTTTTTCgcagca                           >  1:4030663/1‑140 (MQ=255)
                                                                                                                  tGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATCTAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCgcagca                           <  1:3098843/140‑1 (MQ=255)
                                                                                                                  tGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCgcagca                           >  1:296138/1‑140 (MQ=255)
                                                                                                                  tGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCgcagca                           >  1:287899/1‑140 (MQ=255)
                                                                                                                  tGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTGCGTAATGGCCGTTCTTTTTCgcagca                           >  2:3437828/1‑140 (MQ=255)
                                                                                                                    gAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGcaaa                         >  2:2412986/1‑140 (MQ=255)
                                                                                                                              ccGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGcc               >  1:1980977/1‑140 (MQ=255)
                                                                                                                              ccGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGcc               >  1:1401852/1‑140 (MQ=255)
                                                                                                                              ccGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGcc               >  2:1669030/1‑140 (MQ=255)
                                                                                                                              ccGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGcc               >  1:2501561/1‑140 (MQ=255)
                                                                                                                              ccGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGcc               >  1:2037011/1‑140 (MQ=255)
                                                                                                                                         aCAAAATCACAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGa    <  2:759334/140‑1 (MQ=255)
                                                                                                                                         aCAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGa    >  2:2129927/1‑140 (MQ=255)
                                                                                                                                         aCAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGa    <  2:1255045/140‑1 (MQ=255)
                                                                                                                                         aCAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGa    <  1:3634536/140‑1 (MQ=255)
                                                                                                                                           aaaatcaaaatTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGaaa  <  2:2758574/140‑1 (MQ=255)
                                                                                                                                           aaaatcaaaatTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGaaa  <  1:2129927/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
TAAAGAAAGCGGGCAGATTGCCAGCCAAAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAA  >  NZ_CP034943/2775731‑2776009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: