Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,775,897 T→C 6.1% L130L (TTA→TTG)  EO946_RS14350 ← TVP38/TMEM64 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,775,8970TC 6.1% 592.6 / 18.1 179L130L (TTA→TTG) EO946_RS14350TVP38/TMEM64 family protein
Reads supporting (aligned to +/- strand):  ref base T (93/75);  new base C (6/5);  total (99/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 212 total aligned reads displayed.

AAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAATA  >  NZ_CP034943/2775758‑2776036
                                                                                                                                           |                                                                                                                                           
       cGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAaaagaa                                                                                                                                      <  2:1441563/140‑1 (MQ=255)
       agCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAAGACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAaaagaa                                                                                                                                      <  2:2156695/139‑1 (MQ=255)
           cccAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGat                                                                                                                                  <  2:2697752/140‑1 (MQ=255)
              aGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGAtata                                                                                                                               >  2:3697378/1‑140 (MQ=255)
               gCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTCAAAGAAAGATATaa                                                                                                                              >  1:1272672/1‑140 (MQ=255)
                    ttAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATaaaaaaa                                                                                                                         >  2:1578316/1‑140 (MQ=255)
                     ttaGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCTCATCAAAATAAGTCAGCGCTTTGACATTGGAAATACCTGCCGCATAACTGACAAAATCAAAATTGATCGGAAGGATTCTTAAAAGAAAGATATAAAAAAAt                                                                                                                        <  1:1972847/138‑1 (MQ=255)
                     tAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAAt                                                                                                                        >  1:1985054/1‑140 (MQ=255)
                     tAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAAt                                                                                                                        <  2:2824155/140‑1 (MQ=255)
                      aaGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATc                                                                                                                       >  1:3094974/1‑140 (MQ=255)
                      aaGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATc                                                                                                                       >  1:3470950/1‑140 (MQ=255)
                      aaGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATc                                                                                                                       >  1:3901646/1‑140 (MQ=255)
                        gcgcAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATcag                                                                                                                     >  2:3577491/1‑138 (MQ=255)
                         cgacaTAGTCCCAGTGATGATTCCCTCAGCCGTCGCCGCAAAATTCGTCAGCGCTTTGACATTGGAATTAACTGCCGCATAACTGCAAAAATCAAAATTCATCGGCATGATTATTAAAACAAAGATATAAAAAAATCCGt                                                                                                                    <  2:898937/136‑1 (MQ=255)
                           cAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTa                                                                                                                  <  2:665722/140‑1 (MQ=255)
                             atcgTCCCAGGGATGATTCCCACAGCCGTTGCGGCAAAATACGGCAGCGCTTTGACATTGGAAAGGCCTGCCGCATAGCTGACGAAATCAAAATTGATCGGCAGGATTCTCAAAAGAAAGATATAGAAAAATCCGTTAtc                                                                                                                >  1:2640047/4‑140 (MQ=255)
                             atcgTCCCAGGGATGATTCCCACAGCCGTTGCGGCAAAATACGGCAGCGCTTTGACATTGGAAAGGCCTGCCGCATAGCTGACGAAATCAAAATTGATCGGCAGGATTCTCAAAAGAAAGATATAGAAAAATCCGTTAtc                                                                                                                >  1:2346410/4‑140 (MQ=255)
                             aTAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTAtc                                                                                                                >  2:1739758/1‑140 (MQ=255)
                               aGTCCCAGGGATGAGTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTAtcct                                                                                                              <  2:1431078/140‑1 (MQ=255)
                                   ccAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCAt                                                                                                          <  1:3775789/140‑1 (MQ=255)
                                   ccAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCAt                                                                                                          <  1:1243874/140‑1 (MQ=255)
                                   ccAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCAt                                                                                                          >  1:1147946/1‑140 (MQ=255)
                                   ccAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCAt                                                                                                          >  1:2777818/1‑140 (MQ=255)
                                    cAGGGATGATTCCCGCAGCCGTCGCCTCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATc                                                                                                         <  2:2243000/140‑1 (MQ=255)
                                    cAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATc                                                                                                         <  1:2836733/140‑1 (MQ=255)
                                    cAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATc                                                                                                         <  2:4041803/140‑1 (MQ=255)
                                    cAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATc                                                                                                         <  1:2287952/140‑1 (MQ=255)
                                      gggATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTg                                                                                                       >  1:1614952/1‑140 (MQ=255)
                                      gggATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTg                                                                                                       <  2:529441/140‑1 (MQ=255)
                                          tgatTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTc                                                                                                   >  2:3019795/1‑140 (MQ=255)
                                           gatTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAGAAAATCAAAATTGATCGGCAGGAGTCTTAAAAGACAGATATAAAAAAATCGGGTATCCTCCTTCTGCTACt                                                                                                  >  1:3249865/1‑140 (MQ=255)
                                           gatTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTCAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCt                                                                                                  >  2:314260/1‑140 (MQ=255)
                                           gatTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTg                                                                                                       <  1:2223838/135‑1 (MQ=255)
                                           gatTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTg                                                                                                       >  2:2223838/1‑135 (MQ=255)
                                           gatTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCt                                                                                                  >  1:1161842/1‑140 (MQ=255)
                                           gatTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCt                                                                                                  >  1:695720/1‑140 (MQ=255)
                                            atTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATc                                                                                                         >  1:3188992/1‑132 (MQ=255)
                                            atTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTg                                                                                                 >  2:2873725/1‑140 (MQ=255)
                                            atTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTg                                                                                                 >  2:2913583/1‑140 (MQ=255)
                                              tctcGCAGCCGTAGCCGCAAAATACGTCAGAGCTTTTAAATTGGAAAGACCAGCCGAATAACTGACAAAATCAATATTGCTCGGCAGGATTATTAAAATAAAGATATAAAAAAATCCGTTATCCTCCATCTTCTTCTGaa                                                                                               <  2:1871908/137‑1 (MQ=255)
                                              tCCCGCATCCGTCGCCTCAAAATAAGTCAGAGCTTTGACATTTGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGaa                                                                                               <  1:1989245/140‑1 (MQ=255)
                                              tCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGaa                                                                                               <  1:555713/140‑1 (MQ=255)
                                              tCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGaa                                                                                               <  1:890001/140‑1 (MQ=255)
                                              tCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGaa                                                                                               <  1:1444121/140‑1 (MQ=255)
                                               cccGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAAt                                                                                              <  2:3489722/140‑1 (MQ=255)
                                                 cGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTATTAAAAGAAATATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATgg                                                                                            >  2:858223/1‑140 (MQ=255)
                                                   cAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTCAAAGACAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGACTGGCt                                                                                          >  2:372769/1‑140 (MQ=255)
                                                        gTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAg                                                                                     <  1:3379768/140‑1 (MQ=255)
                                                                          aGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTa                                                                                                                  >  1:477978/1‑93 (MQ=255)
                                                                          aGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGtt                                                                   >  2:2789148/1‑140 (MQ=255)
                                                                                       tGGAAAGGCCTGCCGCATAGCTGACGAAATCAAAATTGATCGGCAGGATTCTCAAAAGAAAGATATAGAAAAATCCGTTATCCTCCATTTGCTTCTGTATGGCTTCAAGCCTTTCGTAATGCCCGTTCTTTTTCgccgaa                                                      <  2:2640036/140‑5 (MQ=255)
                                                                                       tGGAAAGGCCTGCCGCATAGCTGACGAAATCAAAATTGATCGGCAGGATTCTCAAAAGAAAGATATAGAAAAATCCGTTATCCTCCATTTGCTTCTGTATGGCTTCAAGCCTTTCGTAATGCCCGTTCTTTTTCgccgaa                                                      <  2:2346410/140‑5 (MQ=255)
                                                                                       tGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCTGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCTTAATGGCCGTTCTTTTTCgaagca                                                      >  1:2315587/1‑140 (MQ=255)
                                                                                       tGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGACAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTCATGGCCGTTCTTTTTCgcagca                                                      >  1:4030663/1‑140 (MQ=255)
                                                                                       tGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCgcagca                                                      >  1:296138/1‑140 (MQ=255)
                                                                                       tGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCgcagca                                                      >  1:287899/1‑140 (MQ=255)
                                                                                       tGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTGCGTAATGGCCGTTCTTTTTCgcagca                                                      >  2:3437828/1‑140 (MQ=255)
                                                                                         gAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGcaaa                                                    >  2:2412986/1‑140 (MQ=255)
                                                                                                 tGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAg                                            >  2:455340/1‑140 (MQ=255)
                                                                                                  gCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGc                                           >  1:2045229/1‑140 (MQ=255)
                                                                                                   ccGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGcc                                          >  2:1669179/1‑140 (MQ=255)
                                                                                                   ccGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGcc                                          >  2:572366/1‑140 (MQ=255)
                                                                                                   ccGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGcc                                          >  1:2501561/1‑140 (MQ=255)
                                                                                                   ccGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGcc                                          >  1:1401852/1‑140 (MQ=255)
                                                                                                   ccGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGcc                                          >  1:4212818/1‑140 (MQ=255)
                                                                                                              aCAAAATCACAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGa                               <  2:759334/140‑1 (MQ=255)
                                                                                                              aCAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGa                               <  1:3634536/140‑1 (MQ=255)
                                                                                                              aCAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGa                               <  2:3807038/140‑1 (MQ=255)
                                                                                                              aCAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGa                               >  1:968527/1‑140 (MQ=255)
                                                                                                              aCAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGa                               <  2:1255045/140‑1 (MQ=255)
                                                                                                              aCAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGa                               >  2:2129927/1‑140 (MQ=255)
                                                                                                                cacatcaaaagAGACCCGCACGATACTCAACACAAAGAGCTAAACCACTCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGaaa                             <  1:2423715/137‑1 (MQ=255)
                                                                                                                   atcaaaatTGATCGGCAGGATTCTTAAAAGAATGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTAGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAATCGAAACAg                          >  2:4092117/1‑140 (MQ=255)
                                                                                                                   atcaaaatTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAg                          >  2:774109/1‑140 (MQ=255)
                                                                                                                     caaaatTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCt                        <  2:705922/140‑1 (MQ=255)
                                                                                                                     caaaatTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCt                        <  2:1878353/140‑1 (MQ=255)
                                                                                                                          ttGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGgc                   <  2:1161842/140‑1 (MQ=255)
                                                                                                                          ttGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGgc                   <  2:4331471/140‑1 (MQ=255)
                                                                                                                          ttGATCGGCAGGATTCATAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGgc                   <  2:3249865/140‑1 (MQ=255)
                                                                                                                           tGATCTGCAGTATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGgcg                  <  2:2342370/140‑1 (MQ=255)
                                                                                                                           tGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGgcg                  <  1:3405034/140‑1 (MQ=255)
                                                                                                                                 gCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATc            <  2:2768525/140‑1 (MQ=255)
                                                                                                                                   aGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGa          <  2:1656217/140‑1 (MQ=255)
                                                                                                                                        tCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTTCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGa     <  1:1672152/140‑1 (MQ=255)
                                                                                                                                        tCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGa     <  2:260074/140‑1 (MQ=255)
                                                                                                                                        tCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGa     <  2:3094974/140‑1 (MQ=255)
                                                                                                                                          ttAAAAGAACGATAGAAAAAAAGCCCTTATCCTCCATATGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAAt   <  2:2483473/140‑1 (MQ=255)
                                                                                                                                          ttAAAAGAAAGATATAAACAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAAt   <  2:1987200/140‑1 (MQ=255)
                                                                                                                                          ttAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGGACCGAAt   <  1:1642797/140‑1 (MQ=255)
                                                                                                                                          ttAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAAt   <  1:1557206/140‑1 (MQ=255)
                                                                                                                                          ttAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAAt   <  1:2938321/140‑1 (MQ=255)
                                                                                                                                          ttAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAAt   <  1:572366/140‑1 (MQ=255)
                                                                                                                                          ttAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAAt   <  1:1669030/140‑1 (MQ=255)
                                                                                                                                          ttAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAAt   <  1:2789148/140‑1 (MQ=255)
                                                                                                                                          ttAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAAt   <  2:3231506/140‑1 (MQ=255)
                                                                                                                                          ttAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAAt   <  2:337717/140‑1 (MQ=255)
                                                                                                                                          ttAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAAt   <  1:2606183/140‑1 (MQ=255)
                                                                                                                                          ttAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAAt   <  1:1797399/140‑1 (MQ=255)
                                                                                                                                           tAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAATa  <  2:3846349/140‑1 (MQ=255)
                                                                                                                                           tAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAATa  <  1:1331811/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
AAAAGCTCGCACCCAGCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAATA  >  NZ_CP034943/2775758‑2776036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑