Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,775,912 A→G 5.9% F125F (TTT→TTC EO946_RS14350 ← TVP38/TMEM64 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,775,9120AG 5.9% 661.0 / 18.7 201F125F (TTT→TTCEO946_RS14350TVP38/TMEM64 family protein
Reads supporting (aligned to +/- strand):  ref base A (86/103);  new base G (6/6);  total (92/109)
Fisher's exact test for biased strand distribution p-value = 7.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 208 total aligned reads displayed.

GCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAATAACGTGTAAAGC  >  NZ_CP034943/2775773‑2776047
                                                                                                                                           |                                                                                                                                       
gCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTCAAAGAAAGATATaa                                                                                                                                         >  1:1272672/1‑140 (MQ=255)
gCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATaa                                                                                                                                         >  1:1987200/1‑140 (MQ=255)
  acaTTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATaaaa                                                                                                                                       <  2:2298479/140‑1 (MQ=255)
     ttAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATaaaaaaa                                                                                                                                    >  2:1438824/1‑140 (MQ=255)
     ttAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATaaaaaaa                                                                                                                                    >  2:1578316/1‑140 (MQ=255)
      tAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAAt                                                                                                                                   <  2:1099837/140‑1 (MQ=255)
       aaGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATc                                                                                                                                  >  1:3094974/1‑140 (MQ=255)
       aaGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATc                                                                                                                                  >  1:3901646/1‑140 (MQ=255)
       aaGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATc                                                                                                                                  >  1:260074/1‑140 (MQ=255)
          cgacaTAGTCCCAGTGATGATTCCCTCAGCCGTCGCCGCAAAATTCGTCAGCGCTTTGACATTGGAATTAACTGCCGCATAACTGCAAAAATCAAAATTCATCGGCATGATTATTAAAACAAAGATATAAAAAAATCCGt                                                                                                                               <  2:898937/136‑1 (MQ=255)
              atcgTCCCAGGGATGATTCCCACAGCCGTTGCGGCAAAATACGGCAGCGCTTTGACATTGGAAAGGCCTGCCGCATAGCTGACGAAATCAAAATTGATCGGCAGGATTCTCAAAAGAAAGATATAGAAAAATCCGTTAtc                                                                                                                           >  1:2640036/4‑140 (MQ=255)
              atcgTCCCAGGGATGATTCCCACAGCCGTTGCGGCAAAATACGGCAGCGCTTTGACATTGGAAAGGCCTGCCGCATAGCTGACGAAATCAAAATTGATCGGCAGGATTCTCAAAAGAAAGATATAGAAAAATCCGTTAtc                                                                                                                           >  1:908238/4‑140 (MQ=255)
              aTAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTAtc                                                                                                                           >  2:1739758/1‑140 (MQ=255)
                    ccAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCAt                                                                                                                     >  1:1147946/1‑140 (MQ=255)
                    ccAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCAt                                                                                                                     >  1:2777818/1‑140 (MQ=255)
                     cAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATc                                                                                                                    <  1:2836733/140‑1 (MQ=255)
                     cAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATc                                                                                                                    <  1:2287952/140‑1 (MQ=255)
                     cAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTGATCCTCCATc                                                                                                                    <  1:3451059/140‑1 (MQ=255)
                     cAGGGATGATGCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCATTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATc                                                                                                                    <  2:2188259/140‑1 (MQ=255)
                       gggATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTg                                                                                                                  >  2:3379768/1‑140 (MQ=255)
                       gggATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTg                                                                                                                  >  2:3099812/1‑140 (MQ=255)
                       gggATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTg                                                                                                                  >  1:2342370/1‑140 (MQ=255)
                       gggATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTg                                                                                                                  >  1:3825516/1‑140 (MQ=255)
                       gggATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTg                                                                                                                  >  1:1614952/1‑140 (MQ=255)
                       gggATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTg                                                                                                                  >  1:1656217/1‑140 (MQ=255)
                           tgatTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTc                                                                                                              >  2:3019703/1‑140 (MQ=255)
                            gatTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGAGAAAATCAAAATTGATCGGCAGGAGTCTTAAAAGACAGATATAAAAAAATCGGGTATCCTCCTTCTGCTACt                                                                                                             >  1:3249865/1‑140 (MQ=255)
                            gatTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTg                                                                                                                  <  1:2223838/135‑1 (MQ=255)
                            gatTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCt                                                                                                             >  1:4331471/1‑140 (MQ=255)
                            gatTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCt                                                                                                             >  1:695720/1‑140 (MQ=255)
                            gatTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCt                                                                                                             >  2:717128/1‑140 (MQ=255)
                            gatTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCt                                                                                                             >  1:1161842/1‑140 (MQ=255)
                             atTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATc                                                                                                                    >  1:3188992/1‑132 (MQ=255)
                             atTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATc                                                                                                                    <  2:3188992/132‑1 (MQ=255)
                             atTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTg                                                                                                            >  2:306650/1‑140 (MQ=255)
                             atTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTg                                                                                                            >  2:2913583/1‑140 (MQ=255)
                             atTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTg                                                                                                            >  2:2787443/1‑140 (MQ=255)
                               tctcGCAGCCGTAGCCGCAAAATACGTCAGAGCTTTTAAATTGGAAAGACCAGCCGAATAACTGACAAAATCAATATTGCTCGGCAGGATTATTAAAATAAAGATATAAAAAAATCCGTTATCCTCCATCTTCTTCTGaa                                                                                                          <  2:1871908/137‑1 (MQ=255)
                               tCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGaa                                                                                                          <  1:563041/140‑1 (MQ=255)
                               tCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGaa                                                                                                          <  1:1444121/140‑1 (MQ=255)
                                  cGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTATTAAAAGAAATATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATgg                                                                                                       >  2:858223/1‑140 (MQ=255)
                                    cAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCt                                                                                                     >  2:372709/1‑140 (MQ=255)
                                         gTCGCCGCAACATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAg                                                                                                <  1:2485877/140‑1 (MQ=255)
                                                    aTACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAAt                                                                                     <  2:64900/140‑1 (MQ=255)
                                                                tttGAGATTGGAAAGACCTGCAGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCtttt                                                                         <  2:2225981/140‑1 (MQ=255)
                                                                        tGGAAAGGCCTGCCGCATAGCTGACGAAATCAAAATTGATCGGCAGGATTCTCAAAAGAAAGATATAGAAAAATCCGTTATCCTCCATTTGCTTCTGTATGGCTTCAAGCCTTTCGTAATGCCCGTTCTTTTTCgccgaa                                                                 <  2:2346410/140‑5 (MQ=255)
                                                                        tGGAAAGGCCTGCCGCATAGCTGACGAAATCAAAATTGATCGGCAGGATTCTCAAAAGAAAGATATAGAAAAATCCGTTATCCTCCATTTGCTTCTGTATGGCTTCAAGCCTTTCGTAATGCCCGTTCTTTTTCgccgaa                                                                 <  2:2640036/140‑5 (MQ=255)
                                                                        tGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGACAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTCATGGCCGTTCTTTTTCgcagca                                                                 >  1:4030663/1‑140 (MQ=255)
                                                                        tGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCgcagca                                                                 >  1:287899/1‑140 (MQ=255)
                                                                        tGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCgcagca                                                                 >  1:296138/1‑140 (MQ=255)
                                                                          gAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGcaaa                                                               >  2:2412986/1‑140 (MQ=255)
                                                                                  tGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAg                                                       >  2:455340/1‑140 (MQ=255)
                                                                                    ccGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGcc                                                     >  1:1980977/1‑140 (MQ=255)
                                                                                    ccGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGcc                                                     >  1:2037011/1‑140 (MQ=255)
                                                                                    ccGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGcc                                                     >  2:1669179/1‑140 (MQ=255)
                                                                                    ccGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGcc                                                     >  1:4212818/1‑140 (MQ=255)
                                                                                    ccGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGcc                                                     >  1:1401852/1‑140 (MQ=255)
                                                                                          aaCTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCaaaa                                               <  2:3245883/140‑1 (MQ=255)
                                                                                               aCAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGa                                          <  1:3389599/140‑1 (MQ=255)
                                                                                               aCAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGa                                          <  1:3634536/140‑1 (MQ=255)
                                                                                               aCAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGa                                          >  2:2129927/1‑140 (MQ=255)
                                                                                               aCAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGa                                          <  2:1255045/140‑1 (MQ=255)
                                                                                                 aaaatcaaaatTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGaaa                                        <  2:2758574/140‑1 (MQ=255)
                                                                                                 aaaatcaaaatTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGaaa                                        <  1:2129927/140‑1 (MQ=255)
                                                                                                    atcaaaatTGATCGGCAGGATTCTTAAAAGAATGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTAGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAATCGAAACAg                                     >  2:4092117/1‑140 (MQ=255)
                                                                                                    atcaaaatTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAg                                     >  2:3738445/1‑140 (MQ=255)
                                                                                                    atcaaaatTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAg                                     >  2:773982/1‑140 (MQ=255)
                                                                                                      caaaatTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCt                                   <  2:1878353/140‑1 (MQ=255)
                                                                                                           ttGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGgc                              <  2:4331471/140‑1 (MQ=255)
                                                                                                           ttGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGgc                              <  2:695720/140‑1 (MQ=255)
                                                                                                            tGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGgcg                             <  2:3825516/140‑1 (MQ=255)
                                                                                                                    aGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGa                     <  2:1656217/140‑1 (MQ=255)
                                                                                                                         tCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGa                <  2:3494958/140‑1 (MQ=255)
                                                                                                                         tCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGa                <  2:3470950/140‑1 (MQ=255)
                                                                                                                         tCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGa                <  2:260074/140‑1 (MQ=255)
                                                                                                                           ttAAAAGAACGATAGAAAAAAAGCCCTTATCCTCCATATGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAAt              <  2:2483473/140‑1 (MQ=255)
                                                                                                                           ttAAAAGAAAGATATAAACAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAAt              <  2:1987200/140‑1 (MQ=255)
                                                                                                                           ttAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACTAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAAt              <  2:525679/140‑1 (MQ=255)
                                                                                                                           ttAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAAt              <  1:500975/140‑1 (MQ=255)
                                                                                                                           ttAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAAt              <  1:238568/140‑1 (MQ=255)
                                                                                                                           ttAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAAt              <  1:2789148/140‑1 (MQ=255)
                                                                                                                           ttAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAAt              <  2:786306/140‑1 (MQ=255)
                                                                                                                           ttAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAAt              <  1:2938321/140‑1 (MQ=255)
                                                                                                                           ttAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAAt              <  1:1677765/140‑1 (MQ=255)
                                                                                                                           ttAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAAt              <  1:1669179/140‑1 (MQ=255)
                                                                                                                           ttAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAAt              <  2:281535/140‑1 (MQ=255)
                                                                                                                           ttAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAAt              <  2:3344800/140‑1 (MQ=255)
                                                                                                                           ttAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAAt              <  2:285148/140‑1 (MQ=255)
                                                                                                                           ttAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAAt              <  1:1642436/140‑1 (MQ=255)
                                                                                                                           ttAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAAt              <  1:1557206/140‑1 (MQ=255)
                                                                                                                           tcaaaaGAAAGATATAGAAAAATCCGTTATCCTCCATTTGCTTCTGTATGGCTTCAAGCCTTTCGTAATGCCCGTTCTTTTTCGCCGAAAACAAACCAGCCGCAAAAAACGAAACAGCTGAAGCGCACATCGAACCGAAt              <  1:178380/138‑1 (MQ=255)
                                                                                                                           atAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAAt              <  1:3306264/139‑1 (MQ=255)
                                                                                                                            tAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAATa             <  1:1331811/140‑1 (MQ=255)
                                                                                                                            tAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAATa             <  2:563265/140‑1 (MQ=255)
                                                                                                                            tAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAATa             <  2:3846349/140‑1 (MQ=255)
                                                                                                                                  aaagaTATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAATAACgtgt       >  2:1359227/1‑140 (MQ=255)
                                                                                                                                     gaTATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAAc                                       <  2:2883569/105‑1 (MQ=255)
                                                                                                                                       tataAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAATAACGTGTAAAGc  <  1:2731415/140‑1 (MQ=255)
                                                                                                                                       tataAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAATAACGTGTAAAGc  <  1:1956249/140‑1 (MQ=255)
                                                                                                                                       tataAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAATAACGTGTAAAGc  <  2:3969433/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                       
GCACATTAAGCGCAATAGTCCCAGGGATGATTCCCGCAGCCGTCGCCGCAAAATACGTCAGCGCTTTGACATTGGAAAGACCTGCCGCATAACTGACAAAATCAAAATTGATCGGCAGGATTCTTAAAAGAAAGATATAAAAAAATCCGTTATCCTCCATCTGCTTCTGAATGGCTTCCAGCTTTTCGTAATGGCCGTTCTTTTTCGCAGCAAACAAACCAGCCGCAAAAAACGAAACAGCTGAGGCGCACATCGAACCGAATAACGTGTAAAGC  >  NZ_CP034943/2775773‑2776047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: