BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,865,472	C→A	8.3%	D116Y (GAT→TAT)	pulA ←	type I pullulanase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,865,472	0	C	A	8.3%	595.4 / 9.2	241	D116Y (GAT→TAT)	pulA	type I pullulanase
Reads supporting (aligned to +/- strand): ref base C (93/118); new base A (27/2); total (121/120)
Fisher's exact test for biased strand distribution p-value = 3.89e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 244 total aligned reads displayed.

AACCGTGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATCAAACGTATCGGTCCGGATGACCGCGCCAATTTGGAGATCCGTTTTGTCGCCGCTGGACGCTCTGACGGCATGTATTTTTCCG  >  NZ_CP034943/2865334‑2865611

aaCCGTGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCa                                                                                                                                            >  2:3106916/1‑140 (MQ=255)

  ccGTGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGt                                                                                                                                          <  1:925324/140‑1 (MQ=255)

  ccGTGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGt                                                                                                                                          <  1:3736955/140‑1 (MQ=255)

  ccGTGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCGGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGt                                                                                                                                          >  2:3926409/1‑140 (MQ=255)

  ccGTGAAGATCACCGGTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGt                                                                                                                                          <  2:1992571/140‑1 (MQ=255)

     tGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGACTATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGtata                                                                                                                                       >  2:1283809/1‑140 (MQ=255)

     tGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGCGGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGtata                                                                                                                                       >  2:1347004/1‑140 (MQ=255)

     tGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGtata                                                                                                                                       >  2:3763375/1‑140 (MQ=255)

     tGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGtata                                                                                                                                       <  1:2835570/140‑1 (MQ=255)

     tGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAACCTTTACAGCAGCGGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGtata                                                                                                                                       >  2:3421189/1‑140 (MQ=255)

     tGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATGTGGAATATGCGGCCGCTTGTATTAGGATGGGAAATCATGACACCAGCGGTGGTTGGCGCAGCGGCCCCTGCTTAAAAATCCGtata                                                                                                                                       >  2:1663663/1‑140 (MQ=255)

     tGAAGATCACCGTTGACAGAAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTTGGGATCGACACCTTGACAACAGCGGAGGGTGCGCCCGGCGCCCACACATTAAAATCAGtata                                                                                                                                       >  1:3579043/1‑140 (MQ=255)

       aaGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTAGGGTGGGAAAGCTTGACAGCAGCTGAGGGTGCGGCAGGCGCCCAAACTTTAAAATCAGTATAAt                                                                                                                                     >  1:2988153/1‑140 (MQ=255)

       aaGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCGTTTATTCGGATGGGTCAGCGTGTCAGCAGCGGAGGTTGGGGCAGGCGCCCATACTTTAAAATCAGTATaag                                                                                                                                     >  1:3834039/1‑139 (MQ=255)

        aGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAAtt                                                                                                                                    <  1:838715/140‑1 (MQ=255)

         taTCACCGTTGACATTAACGGTATAGACTCCTTTTTCAATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATTGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTc                                                                                                                                   <  1:3554363/139‑1 (MQ=255)

         gATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTc                                                                                                                                   <  1:3211806/140‑1 (MQ=255)

         gATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTc                                                                                                                                   <  1:2930625/140‑1 (MQ=255)

         gATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTc                                                                                                                                   <  2:2771146/140‑1 (MQ=255)

         gATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTGATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTc                                                                                                                                   <  2:1507698/140‑1 (MQ=255)

         gATCACCGTTGACAGTAACGGTATAGACGCCTTTTTACATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTc                                                                                                                                   <  2:261037/140‑1 (MQ=255)

          aTCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCg                                                                                                                                  <  1:2277334/140‑1 (MQ=255)

           tCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGc                                                                                                                                 <  2:832835/140‑1 (MQ=255)

           tCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGc                                                                                                                                 <  1:2526863/140‑1 (MQ=255)

           tCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGc                                                                                                                                 <  2:1914070/140‑1 (MQ=255)

           tCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGc                                                                                                                                 <  1:1925855/140‑1 (MQ=255)

           tCACCGTTGACAGTAACGGTATAGACGCCTTTGTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGc                                                                                                                                 <  2:2556817/140‑1 (MQ=255)

            cACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTGGGTTGGGGAAGCTTTACAGCAGCGGAGGTTTGGGCCGGCGCCCAAACGTTAAAATCAGTATAATTAGCa                                                                                                                                >  1:2762499/1‑140 (MQ=255)

            cACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCa                                                                                                                                >  2:3300365/1‑140 (MQ=255)

             aCCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGAGAGCAGCTGAGGTTGCGGCAGGCGCCCATACGTTAAAAGAAGTATAATTCGCAg                                                                                                                               >  2:606131/1‑140 (MQ=255)

             aCCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCAGTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTAGAAATCGCAg                                                                                                                               >  2:1275987/1‑140 (MQ=255)

                   gACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATaaa                                                                                                                         <  2:866620/140‑1 (MQ=255)

                   gACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATaaa                                                                                                                         <  1:95536/140‑1 (MQ=255)

                   gACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATaaa                                                                                                                         <  1:3307142/140‑1 (MQ=255)

                   gACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATaaa                                                                                                                         <  1:3092007/140‑1 (MQ=255)

                   gACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATaaa                                                                                                                         <  1:2138572/140‑1 (MQ=255)

                   gACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATaaa                                                                                                                         <  2:3161833/140‑1 (MQ=255)

                   gACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATaaa                                                                                                                         <  1:164817/140‑1 (MQ=255)

                   gACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATaaa                                                                                                                         <  2:4254298/140‑1 (MQ=255)

                    aCAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTGGGATTGGAAAGATTTAAAGCACCAGAGGTTGCGGGACGCGCCCAAACTATACAATAACGATAAATCTCCGTAaaaaa                                                                                                                        >  1:1253489/1‑139 (MQ=255)

                    aCAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTAGGCGGGGAAAGCTTGACAGCCGCTGAGGTTGCGGCCGGCGCCCCAACATTTAAATCCGTATAATTCGCACTATAAAc                                                                                                                        >  1:736788/1‑140 (MQ=255)

                         aaCGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGGCAACGTGACAGCAGCGGAGGTTGCGGCCCGCGCCCAAAAATTAAAAACAGTATAATTCGCACAATAAACAcccc                                                                                                                   >  1:1167340/1‑140 (MQ=255)

                         aaCGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAATCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGcac                                                                                                                   >  2:2960915/1‑138 (MQ=255)

                          aaggTATAGACGCCTTGTTCCATCCGAATCATTTTGAATATGCGGCCTCTTTTATTCGGATGGTAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGcccc                                                                                                                  <  2:3942783/138‑1 (MQ=255)

                             gTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTTTTGGGCTGGGAAAGCTTGTCAGCAGCTGAGGTTGCGGCAGGCGCCCAAACATTAAAAAAAGTATAATTAGCAGTATAAAAAGCCCCCac                                                                                                               >  1:3798150/1‑139 (MQ=255)

                             gTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAg                                                                                                               <  2:1467361/140‑1 (MQ=255)

                                taGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGctc                                                                                                            >  2:231063/1‑140 (MQ=255)

                                  gACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTGGGGTGGGAAAGCTTGACAGCAGCGGAGGTTGGGGGCGGCGCCCCTAATTTAAAAAAAGTATAATTAGCAGGATAAAAAGCCCCCACCactc                                                                                                          >  1:2802896/1‑140 (MQ=255)

                                     gCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTAGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATAATTATTATTCTCATTATAAACAGCCCCCAGCtctcgtt                                                                                                       <  2:363347/140‑4 (MQ=255)

                                      ccTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTGGGATGGGAAAGCTTGACAGCAGCGGAGGTTGCGGCCGGCGCCCAAACTTTAAAATCACTATAATTCGCACTATAAACAGCCCCCAGCTCTCCGTc                                                                                                      >  1:4052956/1‑140 (MQ=255)

                                      ccTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGGTGGGAAAGCTTTACAGCAGCTGAGGTTTCGGCCGGCGCCCAAACTTTAAAATAAGTATAATTCGCAGTATAAAAAGCCCCCACCTCTCCGTc                                                                                                      >  1:3670597/1‑140 (MQ=255)

                                      ccTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGCTGGGAAAGCTTGAACGCCGCTGGGGTTGCGGCAGGCGCCCATACGTTAAAATAAGTATAATTAGCAGTAAAAAAAGCCCCCACCTCTcccgc                                                                                                      >  2:512749/1‑137 (MQ=255)

                                      ccTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACCGCAGCGGAGGTTGCGGCCGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAAAAGCCCCCACCTCTCCGTc                                                                                                      >  2:3744006/1‑140 (MQ=255)

                                      ccTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTc                                                                                                      >  2:2255730/1‑140 (MQ=255)

                                      ccTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTc                                                                                                      >  2:1010934/1‑140 (MQ=255)

                                      ccTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTAGGATGGGAAAGCTTGTCAGCCGCGGAGGTTGGGGGAGGCGCCCCAACTTTTAAATCAGTATAATTAGCAGTATAAAAAGCCCCCAGCTCTCCGTc                                                                                                      >  1:24380/1‑140 (MQ=255)

                                        tttttCCATCCGAATCATTTGGAATATGCGGCCGCTTTTTTTAGGATGGGGAAGCTTTAAAGCAGCGGGGGTTTGGGCCTGCGCCCCTACGTTTAAATCAGTATAATTAGCAGTATAAACAGCCCCCCGCTCTCCCTCag                                                                                                    >  1:1189038/1‑139 (MQ=255)

                                        tttttCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGCTGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATAAGTATAATTCGCAGTATAAAAAGCCCCCAGCTCTCCCTCaa                                                                                                    >  2:2110745/1‑139 (MQ=255)

                                        tttttCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGCAAGCTTGACAGCAGGTGAGGTTGGGGCAGGCGCCCCTACGTTAAAATCCGTATAATTCGCAGTATAAACAGCCCCCAGCTCTcccgcct                                                                                                    >  2:3531689/1‑135 (MQ=255)

                                        tttttCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTTACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATAAGTATAATTAGCAGTATAAAAAGCCCCCAGCTCTCCGTCat                                                                                                    >  2:1644633/1‑140 (MQ=255)

                                        tttttCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGGTCTCCGTCat                                                                                                    >  2:1831390/1‑140 (MQ=255)

                                        tttttCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATAATTTAAAATCAGTATAATTGGCATTATAAAAAGCCCCCAGCTCTCCGTCat                                                                                                    >  2:1802486/1‑140 (MQ=255)

                                        tttttCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTAGGATGGGAAAGCGTGACAGCAGGTGAGGTTGCGGCAGGCGCCCAGACTTTAAAATCAGTATAATTCGCAGGATAAACATCCCCCCGCTCTCCGTCat                                                                                                    >  2:3659463/1‑140 (MQ=255)

                                        tttttCCATCCGAATCATTTGGAATATGCGGCCGCTGTTATTGGGTGGGGGAAGCGTGACAGCCGCGGTGGTTGCGGCAGGCGCCCCAAATTTAAAATCAGTATAATTGGCAGTATAAACATCCCCCAGCTCTGCggcct                                                                                                    >  1:1669939/1‑136 (MQ=255)

                                        tttttCCATCCGAATCATTTGGAATATGCGGCCGCGTTTTTTGGGAGGGGGAACCGTGCCACCCCCGGGGGTTTGGGCCGGCGCCCCAACTTTAAAAAAACTATAATTAGCAGTATAAACAGCCCCCccctcaccctcaa                                                                                                    >  1:1601003/1‑139 (MQ=255)

                                        tttttCCATCCGAATCATTTGGAATATGCGGCCGCGTTTATTCGGATGGGAAAGCTTGACAGCACCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCACTATAAACAGCCCCCAGCTCTCCGTCat                                                                                                    >  2:11472/1‑140 (MQ=255)

                                               aTCCGAATCATTTGGAATATGCGGCCGCTTTTATTAGGATGGGTAAGCTTTTAAGCAGCTGGGGTTGGGGGGGGCGCCCATACTTTAAAATAAATATAATTAGCAGTATAAAAAGCCCCCAGCTCTCCGTCAGAATaaaa                                                                                             >  1:1435561/1‑140 (MQ=255)

                                                tCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCCTACTTTAAAAACACTATAATTAGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAAt                                                                                            >  2:176648/1‑140 (MQ=255)

                                                tCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAAt                                                                                            <  1:1883065/140‑1 (MQ=255)

                                                 ccGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATg                                                                                           <  1:2036682/140‑1 (MQ=255)

                                                  cGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGa                                                                                          <  2:1106956/140‑1 (MQ=255)

                                                               atatGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATCAAACGTa                                                                             <  2:2050520/140‑1 (MQ=255)

                                                                tatGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATCAAACGTAt                                                                            <  2:1042192/140‑1 (MQ=255)

                                                                tatGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATCAAACGTAt                                                                            <  2:3198291/140‑1 (MQ=255)

                                                                  tGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATCAAACGTATCg                                                                          <  2:1948248/140‑1 (MQ=255)

                                                                  tGCGGCCGCTTGTCTTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAATATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATCAAACGTATCg                                                                          <  2:3560213/140‑1 (MQ=255)

                                                                        cGCTTTTATTCGGATGGGAAACCTTGAGAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTAGCAGTAAAAACAGCCCCCCGCACTCCGTCATAATAAAAATAATCATCAAAAGTATAGGGCCgg                                                                    >  2:3252655/1‑140 (MQ=255)

                                                                                 tCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATCAAACGTATCGGTCCGGATGACcgcg                                                           <  2:1189038/140‑1 (MQ=255)

                                                                                  cGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATCAAACGTATCGGTCCGGATGACCgcgc                                                          <  1:1010934/140‑1 (MQ=255)

                                                                                  cGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATCAAACGTATCGGTCCGGATGACCgcgc                                                          <  1:2255730/140‑1 (MQ=255)

                                                                                     aTGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGcc                                                                                                                    <  2:413837/79‑1 (MQ=255)

                                                                                     aTGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATCAAACGTATCGGTCCGGATGACCGCGCCaa                                                       >  1:1184394/1‑140 (MQ=255)

                                                                                        tgAAAGCTTGACAGCAGCTGAGGTTGCAGCAGGCGCCCATACTTTAAATACGGTATGATCCGCGGTATAAACGGCGCCCAGCTCTCCGTCATAATAAAATTCGTCATCAAACGCTGCCGTCCGGATGACCGCGCCAAttt                                                    >  2:2914442/2‑140 (MQ=255)

                                                                                                 gACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATCAAACGTATCGGTCCGGATGACCGCGCCAATTTGGAGATCCg                                           >  1:4226082/1‑140 (MQ=255)

                                                                                                         cTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATCAAACGTATCGGTCCGGATGACCGCGCCAATTTGGAGATCCGTTTTGTcg                                   >  1:1457802/1‑140 (MQ=255)

                                                                                                                 gCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATTAATCATCAAACGTATCGGTCCGGATGACCGCGCCAATTTGTAGATCCGTTTTGTCGCCGCTGGa                           <  2:238402/140‑1 (MQ=255)

                                                                                                                 gCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATCAAACGTATCGGTCCGGATGACCGCGCCAATTTGGAGATCCGTTTTGTCGCCGCTGGa                           <  2:3345797/140‑1 (MQ=255)

                                                                                                                 gCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATCAAACGTATCGGTCCGGATGACCGCGCCAATTTGGAGATCCGTTTTGTCGCCGCTGGa                           <  1:2859687/140‑1 (MQ=255)

                                                                                                                 gCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATCAAACGTATCGGTCCGGATGACCGCGCCAATTTGGAGATCCGTTTTGTCGCCGCTGGa                           <  1:333684/140‑1 (MQ=255)

                                                                                                                 gCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATCAAACGTATCGGTCCGGATGACCGCGCCAATTTGGAGATCCGTTTTGTCGCCGCTGGa                           <  1:3609651/140‑1 (MQ=255)

                                                                                                                 gCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATCAAACGTATCGGTCCGGATGACCGCGCCAATTTGGAGATCCGTTTTGTCGCCGCTGGa                           <  1:4332271/140‑1 (MQ=255)

                                                                                                                 gCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATCAAACGTATCGGTCCGGATGACCGCGCCAATTTGGAGATCCGTTTTGTCGCCGCTGGa                           <  1:2128332/140‑1 (MQ=255)

                                                                                                                 gCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATCAAACGTATCGGTCCGGATGACCGCGCCAATTTGGAGATCCGTTTTGTCGCCGCTGGa                           <  1:1812195/140‑1 (MQ=255)

                                                                                                                 gCGGCAGGCGCCCAGACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATCAAACGTATCGGTCCGGATGACCGCGCCAATTTGGAGATCCGTTTTGTCGCCGCTGGa                           <  2:1313054/140‑1 (MQ=255)

                                                                                                                        gcgcCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATCAAACGTATCGGTCCGGATGACCGCGCCAATTTGGAGATCCGTTTTGTCGCCGCTGGACGCTCTg                    >  1:1571404/1‑140 (MQ=255)

                                                                                                                               tACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATCAAACGTATCGGTCCGGATGACCGCGCCAATTTGGAGATCCGTTTTGTCGCCGCTGGACGCTCTGACGGc               >  1:4126558/1‑138 (MQ=255)

                                                                                                                                  tttAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATCAAACGTATCGGTCCGGATGACCGCGCCAATTTGGAGATCCGTTTTGTCGCCGCTGGa                           >  2:1539831/1‑123 (MQ=255)

                                                                                                                                  tttAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATCAAACGTATCGGTCCGGATGACCGCGCCAATTTGGAGATCCGTTTTGTCGCCGCTGGa                           <  1:1539831/123‑1 (MQ=255)

                                                                                                                                    tAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATCAAACGTATCGGTCCGGATGACCGCGCCAATTTGGAGATCCGTTTTGTCGCCGCTGGACGCTCTGACGGCATGTAtt        >  2:4107445/1‑140 (MQ=255)

                                                                                                                                       aaTCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATCAACCGTATCGGTCCGGATGACCGCGCCAATTTGGAGATCCGTTTTGTCGCCGCTGGACGCTCTGACGGCATGTAttttt     <  1:3530074/140‑1 (MQ=255)

AACCGTGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATCAAACGTATCGGTCCGGATGACCGCGCCAATTTGGAGATCCGTTTTGTCGCCGCTGGACGCTCTGACGGCATGTATTTTTCCG  >  NZ_CP034943/2865334‑2865611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`