Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,991,262 G→T 24.4% intergenic (+63/‑144) gbsR → / → bslA transcriptional regulator GbsR/biofilm‑surface layer protein BslA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,991,2620GT24.4% 164.0 / 19.5 100intergenic (+63/‑144)gbsR/bslAtranscriptional regulator GbsR/biofilm‑surface layer protein BslA
Reads supporting (aligned to +/- strand):  ref base G (25/48);  new base T (24/3);  total (49/51)
Fisher's exact test for biased strand distribution p-value = 8.89e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTTTTAGGGGG  >  NZ_CP034943/2991127‑2991396
                                                                                                                                          |                                                                                                                                        
ttGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAATCGCCTGGGAAACCGGGCGGTGTTTTGTTGTTGTTTTTGTGACTAATTCAAATTTTaaaa                                                                                                                                      >  2:3085492/1‑140 (MQ=255)
ttGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGGTTGTGTTGTTTTTTTTGTTATTTCTACACAAGTTGaaa                                                                                                                                       >  1:3468233/1‑140 (MQ=255)
  ggCTGAAACGCCTTGCTGAATCGGTGCACAGCGGGTAAATTTTTAAATTTATACCGATTCAAACAACCAAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAt                                                                                                                                    <  1:2120817/140‑1 (MQ=255)
   gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTTTTTTATTACAAAAAATTTTTAAAATa                                                                                                                                   >  1:166935/1‑140 (MQ=255)
   gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTTTTTAATTACTAAAAAATTTaaaaaaa                                                                                                                                   >  1:19781/1‑138 (MQ=255)
    cTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTATTTAAATAATAAAAATATTAAAAATaa                                                                                                                                  >  1:825329/1‑139 (MQ=255)
     tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGTAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTTTGTTATGACTCACAATGTGAAAtataag                                                                                                                                 >  2:3605992/1‑138 (MQ=255)
     tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTTTTTTTTATGTAATTAAAAAAAAATTTAAAAAAACg                                                                                                                                 >  1:4083360/1‑140 (MQ=255)
     tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTTAATAATACAAATTTTAAAAAAACg                                                                                                                                 >  2:1416911/1‑140 (MQ=255)
     tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTTTTTTATTAAAACAAAATTTAAAAATaag                                                                                                                                 >  1:447972/1‑138 (MQ=255)
       aaaCGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACggg                                                                                                                               <  2:3071718/140‑1 (MQ=255)
         aCGGCTTGATAAATCGCTGCAGAGCGGTTAAATTTTAAAATTTATACCTATTCAAAAAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGaa                                                                                                                            <  1:3252270/140‑1 (MQ=255)
         aCGGATTGCTCAATCTGAGCACAGCGGTGAAATTTTTAAATTTATACCGATTTAAACAAAGAAAAACAAAAACGCCAGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGaa                                                                                                                            <  1:1463005/140‑1 (MQ=255)
           ggCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAACACGCCTGGTAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGaaaa                                                                                                                          <  2:4143663/140‑1 (MQ=255)
            gCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGaaaaa                                                                                                                         <  2:876932/140‑1 (MQ=255)
             tttGCTTAATATGTGCAGACCGTGTATATTTTTAAATTTATACCTATTTAAACAAAGAAAAAAAAAAACGCCTGTGAAACCGGGCTTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAAc                                                                                                                        <  2:2486697/139‑1 (MQ=255)
             cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAATCAAAAACGCCTGGGAAACCGGGCGTTTTGTTGTTGTTTTTTTTGTTATTAATACAAATTTTAAAAAAACGGGcaaatc                                                                                                                        >  2:110797/1‑138 (MQ=255)
             cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGACACCGGGCGTGGTTTTGTTGGTTTTTTTTTTATTAATACAAAATTTTAAAATAACGGGgaaaa                                                                                                                         >  1:525395/1‑140 (MQ=255)
             cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTGTTTTTTTGATTTTTATGTAATTACTACCAATTTGAAAAATACGGGaaaaaa                                                                                                                        >  2:817246/1‑139 (MQ=255)
             cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTATTTTTTATGTAATTAATACAAATTTTTAAAATAAGGGaaaaaa                                                                                                                        >  2:1068553/1‑139 (MQ=255)
              ttGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTTTGTAATTAATACAAATTTGTAAAATAACGGAAAAACa                                                                                                                       >  2:1313092/1‑140 (MQ=255)
              ttGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTAATTACTACCAATTTGAAAAATACGGGAAAAACa                                                                                                                       >  2:3178759/1‑140 (MQ=255)
                gCTTAATCGGTGAAGTGCGGGGAAATTTTTAAATTTATACCTATTTAAACAATGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATc                                                                                                                     <  2:1465433/140‑1 (MQ=255)
                gCTGAATCGTCGCAGCGCGGTGAAATTTATAAATCTATACCGAGAGAAACAAAGTCACCCAAACACGCCGCGGACACCGGGCGATTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATc                                                                                                                     <  2:1980151/140‑1 (MQ=255)
                      tCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAACCCGGGCGTGGTGTTGTTGTTTTTTATGTAATTACTACAAATTTGAAAAATACCGGAAAAAAATCCCAttt                                                                                                               >  2:1624505/1‑140 (MQ=255)
                      tCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTATTTTTTTTGTAATTAATACAAAATTTAAAAATAAGGGAAAAACATACCAttt                                                                                                               >  2:1624695/1‑140 (MQ=255)
                            cAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTTTTTTTTATTTAATTTAAAAAAATTTTAAAAAAAAGTGAAAAACAAACCaattaattaa                                                                                                         >  2:2584791/1‑138 (MQ=255)
                            cAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTTTTTTTTTTTTAATTACTAAAAATTTGAAAAATACGGGCAAAAACTGCCCAttacttac                                                                                                          >  2:2734099/1‑134 (MQ=255)
                                   gggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTTTGTATTTAGTACAAAATTTAAAAAAAAGGGGAAAAAAACCCCGTTACTTGCCaaaaat                                                                                                  >  1:2945238/1‑138 (MQ=255)
                                    ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTTTTTAATTACTACAAATTTGTAAAATACGGGCAAAAAATGCCATTTAGTTCCAAAATAtt                                                                                                >  2:2733562/1‑140 (MQ=255)
                                    ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTTTTTAATTTGTACAAATTTTTAAAAAAAGGGGAAAAAATAACAATTAATTTCAAAaaatg                                                                                                >  1:2485035/1‑139 (MQ=255)
                                    ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTAATTACTAAAAATTTTAAAAATAAGGGAAAAACATACCAGTTAATTCCAAAATAtt                                                                                                >  2:968173/1‑140 (MQ=255)
                                    ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAAATACTACAAAATTGAAAAAAACGGGTAAAAAATGCCATTTACTTCCAAAattgt                                                                                                >  2:2433667/1‑137 (MQ=255)
                                    ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTTTTTTTTTTGTTATTAATAAAAATTTTTAAAAAAAGGGAAAAAAAACCCCATTTATTTCAaaaaatt                                                                                                 >  1:804427/1‑137 (MQ=255)
                                    ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTTTGTAATTAGTACCAATTTGAAAAATAATGGAAAATAATGCCATTTACTTCCAAAATAtt                                                                                                >  2:3839974/1‑140 (MQ=255)
                                    ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTATGTAATTACTACAAATTTGAAAAATAAGGGAAAAAAATCCCAGTTACTTGCAAAAAAtt                                                                                                >  2:1671330/1‑140 (MQ=255)
                                    ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTTTTTAATTAGTAAAAAATTGAAAAATACGGGAAAAAAAACCCATGTACTAGCAAAAAAtt                                                                                                >  2:1392834/1‑140 (MQ=255)
                                    ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTTTTTAATTAATACAAAATTTAAAAAAAAGGGAAAAAAAAGCCAATTACGTGCAAAatagt                                                                                                >  2:1667804/1‑138 (MQ=255)
                                    ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTATTTAAATAATACAAAATTTAAAAAAAAGGGAAAAACATACCATTTAATTACAAAAAAtt                                                                                                >  2:2806468/1‑140 (MQ=255)
                                    ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGGGTTTTTTGTTTTTTTTTTAATTTATAAAAAATTTTAAAAAAAAGGAAAAAAAACACAATTAATTGGAAAataat                                                                                                >  1:2885395/1‑138 (MQ=255)
                                     taaaTTTTTAAATTTATACCGATTTAAAAAAATTAAAAAAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATAttt                                                                                               <  2:1820847/139‑1 (MQ=255)
                                     gAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATAttt                                                                                               <  2:3708105/140‑1 (MQ=255)
                                     gAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATAttt                                                                                               <  2:223713/140‑1 (MQ=255)
                                          ttttAAATTTATACCGATTTAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATgg                                                                                          <  1:1828808/140‑1 (MQ=255)
                                          ttttAAATTTATACCGATTGAAACAACGTAACACAAACACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTGGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATgg                                                                                          <  2:3601884/140‑1 (MQ=255)
                                          ttttAAATTTATACCGATTGAAACAACGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATgg                                                                                          <  1:1828464/140‑1 (MQ=255)
                                          tttcacaTTGATACCCATTCAACCACAGACAAACAAACACCCCTGCGACACCGGCCGTTTTTTTGTTCATTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATgg                                                                                          <  1:2417057/134‑1 (MQ=255)
                                          ttatactttttTACCTATTGAAAAAACGTAAAACAAAAACGCCTGGGAAAGCGGGAGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATGTTAAAAATAATGGAAAAACATCCCATTTACTTCCCACATATTTGATgg                                                                                          <  2:2045467/133‑1 (MQ=255)
                                                  ttATACCTATTTAAAAAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTa                                                                                  <  2:2300034/140‑1 (MQ=255)
                                                  ttATACCGATTGAAACAAACTACAACAAACACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTa                                                                                  <  2:323989/140‑1 (MQ=255)
                                                    ataCCGATTGAAACAAAGTAAAACAAAAACGCCCGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAACACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATg                                                                                <  1:3034934/140‑1 (MQ=255)
                                                    ataCCGATTCAAACAAAGTAAAACAAACACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATg                                                                                <  1:1713623/140‑1 (MQ=255)
                                                    ataCCGATTCAAACAAACTAAAACAATAACGCCTTGGAAACCGGTCGTTTTTTTGTTCTTTTTTTTTGAATTACTACAAATTTGAAAAATACGGGAACAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATg                                                                                <  1:2906400/140‑1 (MQ=255)
                                                     taCCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTTTTTAATTTCTACAAAATTGAAAAATACGGGAAAAACATACCAGTTTCTTGCCAAATATTTTATTGTAATTTTTTGt                                                                               >  2:3411008/1‑140 (MQ=255)
                                                     taCCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTCTTGTTTTTGTAATTACTAAAAATTTTAAAAAAAAGGGAAAAAAAAACCAATTAATTAAAAAAAATTTGTTTGTAATTTTTTGt                                                                               >  2:3134600/1‑140 (MQ=255)
                                                          aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTTTTTAATTAATAAAAAATTTAAAAATAAAGGAAAAAAAAACCATTTAATTAAAAAAGAATTGATATTAAATTTTTGTAGGTa                                                                          >  2:1626791/1‑140 (MQ=255)
                                                          aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTATGTAATTACTAAAAAATTGAAAAATAAGGGACAAAAATACCAATTTCATCCACAATATTTTATGGTAATATTATTTAGGTa                                                                          >  2:8240/1‑140 (MQ=255)
                                                          aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTATTTTTTTTGTAAATAATAAAAAATTGAAAAAAAAGGGAAAAACAAACCCATTACTTCCAAAAAATTTGTTGGTAATTTTATGTTGGTa                                                                          >  2:1465788/1‑140 (MQ=255)
                                                              aaaaaaACTAACACAAAAACGCCTGGGACACCGGGCGTTTTTTTGTTCTTTTTTATGTAAGTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACGTCCAAAATAATTGATGGTAATTTTATGTAGGTAAAAt                                                                      <  1:1549644/140‑1 (MQ=255)
                                                              aaaCAAAGTAACACAAAAACGCCTGTGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAAAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                                      <  2:1422260/140‑1 (MQ=255)
                                                              aaaCAAAGTAAAACAAACACGCCTGGGAAACCGCGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                                      <  2:2796459/140‑1 (MQ=255)
                                                                    aGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGGTGTTTTTTTTTTTATTAATACAAAATTTTAAAATAAGGGAAAAAAAAACCAATTAATTACAAACTATTTTGTTGTTATTTTATGTTTTTAAAAttttttt                                                                >  1:1844047/1‑136 (MQ=255)
                                                                        aaacaaacACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                            <  2:4269372/140‑1 (MQ=255)
                                                                        aaacaaacACGACTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                            <  2:2763119/140‑1 (MQ=255)
                                                                        aaaaaaACACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                            <  1:1667804/140‑1 (MQ=255)
                                                                        aaaaaaACACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                            <  1:1416911/140‑1 (MQ=255)
                                                                        aaaCACAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAACACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                            <  2:3468233/140‑1 (MQ=255)
                                                                        aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                            <  1:1392834/140‑1 (MQ=255)
                                                                        aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                            <  2:722703/140‑1 (MQ=255)
                                                                        aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAAGTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                            <  2:1131545/140‑1 (MQ=255)
                                                                        aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTATTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                            <  2:4221343/140‑1 (MQ=255)
                                                                        aaaCAAAAACGCCTGGGAAACCGCGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                            <  1:565349/140‑1 (MQ=255)
                                                                         aaCAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTTTTTAATTTATACAAAATTTAAAAATAAGGGAAAAACAACCCAGTTAATTCCCAAATATTTGTTGGTAATTTTTTTTATGTTAAATTTGTAttttgg                                                           >  2:3733196/1‑137 (MQ=255)
                                                                         aaCAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTTTTTAATTAAAACAAAATTGAAAAAAAAGGGCAAAACAAACCATTTAAATCCCAAATATTTTTTTGTTATTTTATTTAGGTTAAATTTTTAttttgg                                                           >  2:3733197/1‑137 (MQ=255)
                                                                           cAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTTAAAAATACGCGACAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCg                                                         <  2:3622160/140‑1 (MQ=255)
                                                                             aaaaCGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGtt                                                       <  2:4083360/140‑1 (MQ=255)
                                                                             aaaaCGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGtt                                                       <  2:447972/140‑1 (MQ=255)
                                                                             aaaaCGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGtt                                                       <  1:4023896/140‑1 (MQ=255)
                                                                                ccGCCTGGGAACCCGGGCGTTGTTTTTTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACAACCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCag                                                    <  2:3602075/139‑1 (MQ=255)
                                                                                aCGCCTGGTAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCag                                                    <  1:3411008/140‑1 (MQ=255)
                                                                                aCGCCTGGGAAACCGGGAGTATTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCag                                                    <  1:3134600/140‑1 (MQ=255)
                                                                                  gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                                  <  1:2887856/140‑1 (MQ=255)
                                                                                               ggCGTTTTTTTGTTGGTTTTTATGTAATTTCTTAAAATTTGTAAAATAACGGTAAAACAACCCATTTAATTACAAAATATTTTTAGGTAATTTTATGTAGGTAAAAAGTGTATTTGGGCGGTAAGAGGAAGTGAAAAGCg                                     >  2:1070884/1‑140 (MQ=255)
                                                                                               ggCGTTTTTTTGTTCTTTTTTAAGTAATTAATACAAAATTTAAAAATACGGGAAAAAAATCCCATTTTGATGCAAAATATTTGATGGTTATTTTATGTATGTAAAATTTTTTTTTTGGCGTTATCTGGAGGTGTAAATCg                                     >  2:1051112/1‑140 (MQ=255)
                                                                                                 cttttttttGTTGTTTTTTATGTAATTACTACAAATTTTAAAAATACGGGAAAAACATCCCATTTACTTACAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                                   <  1:156165/138‑1 (MQ=255)
                                                                                                            tCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACAGCCCATTTACTTCCAAAATATGTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAat                        <  1:1235693/140‑1 (MQ=255)
                                                                                                              ttttttATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAatat                      <  2:2403552/140‑1 (MQ=255)
                                                                                                                          ttACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACttt          >  1:2212365/1‑140 (MQ=255)
                                                                                                                               acAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTACTTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGACTATTTTTATAACTTTTTAgg     >  1:2757337/1‑140 (MQ=255)
                                                                                                                               acAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTTTTAgg     <  2:4102030/140‑1 (MQ=255)
                                                                                                                               acAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTTTTAgg     >  1:1081713/1‑140 (MQ=255)
                                                                                                                               acAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTTTTAgg     <  2:4102179/140‑1 (MQ=255)
                                                                                                                               acAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTTTTAgg     >  1:2329904/1‑140 (MQ=255)
                                                                                                                               acAAATTTGAAAAATACGGGAAAAACATCCCAATTACTTCCAAAAGATTTGATGGTAATTTTATGTAGGTGAAATTAGTATTTGGCCGTTCAGAGGAGGGGAAAAGCGGGCAGGCCGGAATATTTTTATACCTTTTTAgg     >  1:4017443/1‑140 (MQ=255)
                                                                                                                                  aaaTTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATTGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTTTTAgggg   <  1:1152391/140‑1 (MQ=255)
                                                                                                                                  aaaTTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTTTTAgggg   <  1:2733562/140‑1 (MQ=255)
                                                                                                                                  aaaTTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTTTTAgggg   <  1:1010919/140‑1 (MQ=255)
                                                                                                                                    aaTTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAACTTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGACTATTTTTAGAACTTTTTAggggg  >  2:141542/1‑140 (MQ=255)
                                                                                                                                    aaTTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCGTGAATATTTTTATAACTTTTTAggggg  >  2:3603701/1‑140 (MQ=255)
                                                                                                                                    aaTTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGCCCGGCCTGAATATTTTTATACCTTTTTAggggg  >  2:3770092/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                        
TTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTTTTAGGGGG  >  NZ_CP034943/2991127‑2991396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: