Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,247,065 C→T 100% intergenic (‑11/+110) bdbD ← / ← cadA disulfide bond formation protein BdbD/cadmium‑translocating P‑type ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,247,0650CT100.0% 839.1 / ‑6.6 247intergenic (‑11/+110)bdbD/cadAdisulfide bond formation protein BdbD/cadmium‑translocating P‑type ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base T (134/108);  minor base G (3/1);  total (137/110)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 247 total aligned reads displayed.

GTCCGGAAACCGCTTCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTCATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTGAGACGCATGA  >  NZ_CP034943/3246928‑3247197
                                                                                                                                         |                                                                                                                                    
    ggAAACCGCTTCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGtt                                                                                                                                >  1:1726619/1‑140 (MQ=255)
        aCCGCTTCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTttaa                                                                                                                            >  2:570844/1‑140 (MQ=255)
        aCCGCTTCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTttaa                                                                                                                            >  2:439896/1‑140 (MQ=255)
        aCCGCTTCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTttaa                                                                                                                            >  2:2418965/1‑140 (MQ=255)
        aCCGCTTCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTAACTCCGACACCTTATCCTTttaa                                                                                                                            >  2:3626579/1‑140 (MQ=255)
          cGCTTCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTttaaaa                                                                                                                          <  2:1431985/140‑1 (MQ=255)
           gCTTCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTttaaaat                                                                                                                         >  2:640276/1‑140 (MQ=255)
           gCTTCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTttaaaat                                                                                                                         >  2:2444262/1‑140 (MQ=255)
             ttCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTttaaaatta                                                                                                                       >  1:3562912/1‑140 (MQ=255)
             ttCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTttaaaataa                                                                                                                       >  1:4150238/1‑138 (MQ=255)
                           ccGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGa                                                                                                         <  2:1173356/140‑1 (MQ=255)
                           ccGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGa                                                                                                         <  2:2906813/140‑1 (MQ=255)
                            cGTTTTGTTATTAAAGATGACAATGGCTGCTATTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                        <  1:253724/140‑1 (MQ=255)
                            cGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                        <  2:946873/140‑1 (MQ=255)
                            cGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                        <  1:3677337/140‑1 (MQ=255)
                            cGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                        <  2:1631752/140‑1 (MQ=255)
                            cGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                        <  1:1255686/140‑1 (MQ=255)
                            cGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                        <  1:1761892/140‑1 (MQ=255)
                            cGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                        <  1:1782646/140‑1 (MQ=255)
                            cGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGAAAACAACCACAACCGTAAGGATGACCGCGAATTTCGCATAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAATTTAAAAAAAGAAGCGGAt                                                                                                        <  1:687592/140‑1 (MQ=255)
                            cGTTTTGTTATTAAAGATGACAATGGCTGCTAGAAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                        <  1:1316972/140‑1 (MQ=255)
                            cGTTCTTTTATTAAAGATGACAATTGCTGCTAGTAAGACAACAACCAGATGCTTATGGATGACCTCGTTTTTCGCAGACGACTGCTTTTTCTTTTTCACTCCTACACCTTATCTTTTTAAAATTAAAAAAAGAAGGGGAt                                                                                                        <  2:3286763/140‑1 (MQ=255)
                             gTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGAAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATc                                                                                                       <  1:3043309/140‑1 (MQ=255)
                              ttttGTTATTAAAGATGACAATGGCTGCTAGTAATACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCg                                                                                                      >  1:2011841/1‑140 (MQ=255)
                              ttttGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCg                                                                                                      >  1:945795/1‑140 (MQ=255)
                              ttttGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGAGGATCg                                                                                                      >  1:3268776/1‑140 (MQ=255)
                              ttttGTTATGAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCg                                                                                                      >  1:491319/1‑140 (MQ=255)
                                  gTTTTTAAAGATGACAATGGCTGCTAGTAACACAACCACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAAt                                                                                                  <  1:2392207/140‑1 (MQ=255)
                                  gTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAAt                                                                                                  <  1:3699425/140‑1 (MQ=255)
                                  gTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAAt                                                                                                  <  1:1977704/140‑1 (MQ=255)
                                  gTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGGAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAAt                                                                                                  <  1:435743/140‑1 (MQ=255)
                                   ttattaAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATa                                                                                                 >  2:1778264/1‑140 (MQ=255)
                                     attaAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGa                                                                                               <  1:1914992/140‑1 (MQ=255)
                                        aaaGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTGATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGAccc                                                                                            >  2:1973136/1‑140 (MQ=255)
                                        aaaGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTCTTTCTTTTACACTCCGAAACCTTATCGGTTTAAAATTAAAAAAAGAAGCGGAACGCAATAGAccc                                                                                            >  2:1496495/1‑140 (MQ=255)
                                         aaGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCa                                                                                           <  2:1660665/140‑1 (MQ=255)
                                         aaGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCa                                                                                           <  2:3083741/140‑1 (MQ=255)
                                              gACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCActtct                                                                                      <  2:3116916/140‑1 (MQ=255)
                                                  aTGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTAt                                                                                  <  1:1943856/140‑1 (MQ=255)
                                                  aTGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTAt                                                                                  <  2:1252695/140‑1 (MQ=255)
                                                  aTGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTAt                                                                                  <  1:226131/140‑1 (MQ=255)
                                                  aTGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGATTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTAt                                                                                  <  2:1098021/140‑1 (MQ=255)
                                                  aTGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTCATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTAt                                                                                  <  1:1110318/140‑1 (MQ=255)
                                                       tgctAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTaa                                                                             <  1:3614962/140‑1 (MQ=255)
                                                        gctAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAac                                                                            <  1:4113634/140‑1 (MQ=255)
                                                        gctAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAac                                                                            <  2:413058/140‑1 (MQ=255)
                                                         ctAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAaca                                                                           >  2:4055293/1‑140 (MQ=255)
                                                             tAAGACAACACCCACCACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAacaagac                                                                       <  1:401721/140‑1 (MQ=255)
                                                               aGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAacaagacaa                                                                     >  2:2733515/1‑140 (MQ=255)
                                                                    acaaccacaaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGcc                                                                >  1:713339/1‑140 (MQ=255)
                                                                     caaccacaaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGa                                                                                                         <  1:2487203/98‑1 (MQ=255)
                                                                     caaccacaaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCt                                                               >  2:2481189/1‑140 (MQ=255)
                                                                      aaccacaaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCta                                                              >  1:2983122/1‑140 (MQ=255)
                                                                        ccacaaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATATACCCACTTCTTTATTTTAACAAGACAAGTGCCtata                                                            >  1:2091034/1‑140 (MQ=255)
                                                                        ccacaaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCCGAGCGCAATAGACCCACTTCTTTATATTAACAAGACAAGTGCCtata                                                            >  2:124003/1‑140 (MQ=255)
                                                                            aaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCCATAGACCCACTTCTTTATTTTAAAAAGTAAAGTGTCTAgaagaa                                                        >  2:2422058/1‑140 (MQ=255)
                                                                            aaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGaa                                                        >  2:3009725/1‑140 (MQ=255)
                                                                            aaccGTAAGGATGACCGCGAATTTAGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGaa                                                        <  2:2335294/140‑1 (MQ=255)
                                                                             accGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGaaa                                                       >  2:4216544/1‑140 (MQ=255)
                                                                                gTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTAtttt                                                                               >  1:3113464/1‑113 (MQ=255)
                                                                                 tAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTATATTTTAACAAGACAAGTGCCTATAAGAAAAacc                                                   >  2:892966/1‑140 (MQ=255)
                                                                                  aaGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTGATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAAcccc                                                  >  2:1357509/1‑140 (MQ=255)
                                                                                        gACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaa                                            >  2:3211549/1‑140 (MQ=255)
                                                                                        gACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaa                                            >  1:1003022/1‑140 (MQ=255)
                                                                                        gACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaa                                            >  2:2764945/1‑140 (MQ=255)
                                                                                        gACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaa                                            >  1:57458/1‑140 (MQ=255)
                                                                                        gACCGCGAATTTCGCAGAAGACTGCCGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaa                                            >  2:3150657/1‑140 (MQ=255)
                                                                                        gACCGCGAATTTCGCAGAAGACTCCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATATTAACAAGACAAGTGCCTATAAGAAAAACCCGaaaaa                                            >  2:1947382/1‑140 (MQ=255)
                                                                                         aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaacaaa                                           >  2:3248161/1‑140 (MQ=255)
                                                                                         aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                           >  1:2925005/1‑140 (MQ=255)
                                                                                         aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                           >  1:149456/1‑140 (MQ=255)
                                                                                         aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                           >  1:383067/1‑140 (MQ=255)
                                                                                         aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                           >  2:3888467/1‑140 (MQ=255)
                                                                                         aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                           >  2:3726040/1‑140 (MQ=255)
                                                                                         aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCATATAATAAAACCCCGaaaaaa                                           >  2:1500905/1‑140 (MQ=255)
                                                                                         aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTGTAACAAGACAAGTGCCTCTAAGAAAACCCCCaaaaaa                                           >  2:3481590/1‑140 (MQ=255)
                                                                                         aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTATTAAAATTAAAAAAATAAGCGGATCGCACTAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACACCGaaaaaa                                           >  1:647821/1‑140 (MQ=255)
                                                                                         aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACGCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCTCAATAGACCCACTTCTTTATTTTAACAAGACAAGAGCCTATAAGAAAACCCCGaaaaaa                                           >  2:2167568/1‑140 (MQ=255)
                                                                                           cgcgAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGt                                                                   <  2:2059503/114‑1 (MQ=255)
                                                                                             cgAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGaa                                       <  2:1709402/140‑1 (MQ=255)
                                                                                             cgAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGaa                                       <  2:3641008/140‑1 (MQ=255)
                                                                                               aaTTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAAtt                                     >  2:3804029/1‑140 (MQ=255)
                                                                                                              tgctgTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTa                      >  1:3149085/1‑140 (MQ=255)
                                                                                                              tgctgTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTa                      >  2:3905937/1‑140 (MQ=255)
                                                                                                              tgctgTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTa                      >  2:3045712/1‑140 (MQ=255)
                                                                                                                ctgTTTCTTTTTCACTCCGACACCTTATCGTTGTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACCATTTATc                    >  2:738588/1‑140 (MQ=255)
                                                                                                                   tttCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTAtttt                                                                               >  1:1828314/1‑78 (MQ=255)
                                                                                                                   tttCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTAtttt                                                                               <  2:1828314/78‑1 (MQ=255)
                                                                                                                        ttttCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGTAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTg            >  1:619000/1‑140 (MQ=255)
                                                                                                                        ttttCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACACTTTATCCGTTTTTg            >  1:2688765/1‑140 (MQ=255)
                                                                                                                        ttttCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTg            >  1:3963049/1‑140 (MQ=255)
                                                                                                                        ttttCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTg            >  1:1142986/1‑140 (MQ=255)
                                                                                                                        ttttCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTg            >  1:789517/1‑140 (MQ=255)
                                                                                                                         tttCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTga           <  1:2791387/140‑1 (MQ=255)
                                                                                                                         tttCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTga           <  1:2871372/140‑1 (MQ=255)
                                                                                                                          ttCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTgag          <  2:491319/140‑1 (MQ=255)
                                                                                                                            caatCCGACACCTGATCTTTTTAAAATTAAAAAAACAAGCGGATCGCTATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCGCTCCGACAATTTATCCGTTTTTGAGAc        <  1:3331113/137‑1 (MQ=255)
                                                                                                                             aCTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTGAGACg       >  2:490655/1‑140 (MQ=255)
                                                                                                                                ccGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTGAGACGCAt    <  2:2803975/140‑1 (MQ=255)
                                                                                                                                  gACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTGAGACGCATGa  <  1:1521334/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                    
GTCCGGAAACCGCTTCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTCATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTGAGACGCATGA  >  NZ_CP034943/3246928‑3247197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: