Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,268,712 G→T 9.5% intergenic (+60/+150) catR → / ← EO946_RS16920 catDE operon transcriptional regulator CatR/HAMP domain‑containing histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,268,7120GT 9.5% 576.3 / 18.6 237intergenic (+60/+150)catR/EO946_RS16920catDE operon transcriptional regulator CatR/HAMP domain‑containing histidine kinase
Reads supporting (aligned to +/- strand):  ref base G (57/152);  new base T (25/2);  total (82/155)
Fisher's exact test for biased strand distribution p-value = 4.00e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 238 total aligned reads displayed.

AAGGCGTTGGCCCCCATCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTCCAGATAG  >  NZ_CP034943/3268578‑3268851
                                                                                                                                      |                                                                                                                                           
aaggCGTTGGCCCCCATCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTGATTTTGCAgg                                                                                                                                        >  1:1534515/1‑140 (MQ=255)
  ggCGTTGGCCCCCATCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTAATTTGCCAGGTa                                                                                                                                      >  2:1393658/1‑140 (MQ=255)
   gCGTTGGCCCCCATCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTTTTTTTGATTTGGCAGGTaa                                                                                                                                     >  1:3250789/1‑140 (MQ=255)
      ttGGCCCCCATCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTGATTTTGCAGGTaaaaa                                                                                                                                  >  1:510773/1‑140 (MQ=255)
      ttGGCCCCCATCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTGTTTTTATTTTTAATTTGGCCGGGaaaaa                                                                                                                                  >  1:3624826/1‑140 (MQ=255)
          cccccATCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGa                                                                                                                              <  2:10639/140‑1 (MQ=255)
              cATCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTTCCAGGGAAAAAACGACtta                                                                                                                          >  1:927076/1‑139 (MQ=255)
                tattGGTTGAGATTTCTACATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGATAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTtctc                                                                                                                        <  2:1118454/138‑1 (MQ=255)
                tCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTtctc                                                                                                                        <  1:1262795/140‑1 (MQ=255)
                tCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTtctc                                                                                                                        <  2:1778422/140‑1 (MQ=255)
                tCTGGGGTGAGTTTTCTAACTGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTtctc                                                                                                                        <  1:178283/140‑1 (MQ=255)
                   tgtgTGAGATTTCTAAAGTGGCGACGGATTGGATTGACCCTTCCTTTGTTTACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                     <  1:3047670/137‑1 (MQ=255)
                   tGGGTGAGATTTCTAAATGGGCGACGGTTTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                     <  1:3955563/140‑1 (MQ=255)
                   tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCGTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                     <  1:1012573/140‑1 (MQ=255)
                   tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                     <  2:956487/140‑1 (MQ=255)
                   tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                     <  1:2998526/140‑1 (MQ=255)
                   tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                     <  2:737407/140‑1 (MQ=255)
                   tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                     <  1:3373400/140‑1 (MQ=255)
                   tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                     <  2:4024726/140‑1 (MQ=255)
                   tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                     <  2:722185/140‑1 (MQ=255)
                   tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATGGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                     <  1:1189621/140‑1 (MQ=255)
                   tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAAGTGCTAAAACGGTTCTGATCTCAAACGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                     <  2:1810248/140‑1 (MQ=255)
                   gtggTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTATTTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                     <  2:3007311/138‑1 (MQ=255)
                     ggTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCg                                                                                                                   <  1:906489/140‑1 (MQ=255)
                     ggTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCg                                                                                                                   <  1:3027417/140‑1 (MQ=255)
                     ggTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCg                                                                                                                   <  1:1983708/140‑1 (MQ=255)
                     ggTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCg                                                                                                                   <  1:1957063/140‑1 (MQ=255)
                     ggTGAGATTTCTAAATGGGCGACGGAGTGGATGGACCGTTCCTTTCTTGACTAATTCCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCg                                                                                                                   <  2:4324297/140‑1 (MQ=255)
                     ggTGAGATTTCTAAAGGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCg                                                                                                                   <  1:4323157/140‑1 (MQ=255)
                      gTGAGATTTCTAAATGGGCGACGGATTGTATTGACCCTTCCTTTCTTGAATAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGa                                                                                                                  <  2:3104207/140‑1 (MQ=255)
                      gTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGa                                                                                                                  <  2:3514908/140‑1 (MQ=255)
                        gagaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGata                                                                                                                <  1:830259/140‑1 (MQ=255)
                        gagaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGata                                                                                                                <  1:770112/140‑1 (MQ=255)
                        gagaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGata                                                                                                                <  2:1656943/140‑1 (MQ=255)
                        gagaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGata                                                                                                                <  2:1599612/140‑1 (MQ=255)
                        gagaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGata                                                                                                                <  1:2153152/140‑1 (MQ=255)
                          gaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGatata                                                                                                              <  2:3227012/140‑1 (MQ=255)
                          gaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGatata                                                                                                              <  2:2383897/140‑1 (MQ=255)
                           aTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAAACACATCTCAGCCGatataa                                                                                                             >  2:1929124/1‑139 (MQ=255)
                           aTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTGTTTTTATTTTTGATTTTCCAGGTAAAAAAACACATCTAACCCGatatat                                                                                                             >  1:2442520/1‑140 (MQ=255)
                             ttCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTCATAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATAtag                                                                                                           <  1:2291031/140‑1 (MQ=255)
                             ttCTAAATGGGCGACGGATTGGAGTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGAATTCTCAGCCGATATAtag                                                                                                           <  2:3939271/140‑1 (MQ=255)
                               cTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTAATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATAtagta                                                                                                         >  2:4134749/1‑140 (MQ=255)
                                      ggCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGGCTTCTCAGGCGATATATAGTAGAAaccc                                                                                                  >  2:2720392/1‑140 (MQ=255)
                                      ggCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAAcccc                                                                                                  >  2:2174504/1‑140 (MQ=255)
                                        cGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGGTAAAAAAGAATTATCAGCCGATATATATTAGAAcccccc                                                                                                >  1:2965356/1‑138 (MQ=255)
                                                  gattgACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGtttt                                                                                      <  1:2211838/140‑1 (MQ=255)
                                                       aCCCTTCCTTTCTTGAGTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTTTTGTTGCTTTGCCAGGTAAAAAACGACGTCTCCGCCGCTATAAAGTGGAACCCCCCATTGCGTTTTTTGcc                                                                                 >  1:1219010/1‑140 (MQ=255)
                                                       aCCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCATAGGCGAGAAAAGAGCCGTGTTTTTATTTTTCATTTTCCAGGTGAAAAAAGACTTCTCACCCCCTATATAGTAGAAACCCACAGAGGGTTTTTAggc                                                                                 >  1:4011643/1‑138 (MQ=255)
                                                       aCCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAATATCCGTGTTTTTATTTTTGATTTGCCAGGTAAAAAACGCCTTATCAGACGATATATAGTTGAACCCCACATTTCGTTTTTAGcc                                                                                 >  2:189165/1‑140 (MQ=255)
                                                       aCCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGGCAGGTAAAAAAAGAATTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTTGcc                                                                                 >  2:323766/1‑140 (MQ=255)
                                                       aCCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAAAGACTTATCAGCCGAAATATAGTAAAAACCCAAATTTCGTTTTTATcc                                                                                 >  2:3287150/1‑140 (MQ=255)
                                                       aCCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTGTTTTTATTTTTAATTTTGCAGGTAAAAAAACACTTGTCAGCCGATATATATTAGAACCCCACATTGCGTTTTTATcc                                                                                 >  1:562606/1‑140 (MQ=255)
                                                         ccttccttTCTTGAATAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCga                                                                               <  1:3754144/140‑1 (MQ=255)
                                                            tccttTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTGATTTTGCCGGGAAAAAACGACTTGTCAGCCGCTATATAGTAGTAACCCACCGTGCGTTTTTAGGCgaccg                                                                            >  1:3272059/1‑137 (MQ=255)
                                                            tccttTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTGATTTGGCAGGTAAAAAAAGACTTCTCAGCCGATATATAGTAGAACCCCACATTTCGTTTTTTTCCgaaga                                                                            >  2:3754312/1‑140 (MQ=255)
                                                            tccttTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTGTTTTTATTTTTTATTTTCCCGGTAAAAAAAAACTTATCACCCGCTATATAGTATAACCCCACCTAGCCTTTTTATCCggaaa                                                                            >  1:1246881/1‑136 (MQ=255)
                                                                 tCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTTATTTTGCATGTAAAAAAAGACTTGTGAGCCGATATATAGTAGAAACCCACATTTCGTTTTTTTCCgaagctttgt                                                                       >  1:3205219/1‑134 (MQ=255)
                                                                          aTTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTAAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGACTGTGAGCAGCTgg                                                              >  2:2255497/1‑140 (MQ=255)
                                                                          aTTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAAACAATTATAAGCCGATATATAGTATAACCCCACATTTCCTTTTTAGCCGGAGAAATTGAGCAGCTgg                                                              >  2:2759297/1‑140 (MQ=255)
                                                                                     ggTTCTGATCTCAACGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCtt                                                   <  1:3081992/140‑1 (MQ=255)
                                                                                     ggTTCTGATCTCAAAGGCGATAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCtt                                                   <  1:1365114/140‑1 (MQ=255)
                                                                                     ggTTCTGATCACAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACTACTTCTCAGCCGATATATACTAGAACCCCACATTGCGTTTTTAGCCTAAGAATTTGAGCAGCTGGCGAAAGAGCtt                                                   <  2:2465335/140‑1 (MQ=255)
                                                                                       ttCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCa                                                 >  2:1249583/1‑140 (MQ=255)
                                                                                       ttCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACAGTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCa                                                 <  2:2823105/140‑1 (MQ=255)
                                                                                       ttCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATGTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCa                                                 <  2:1525880/140‑1 (MQ=255)
                                                                                        tCTTATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCAt                                                <  1:3406219/140‑1 (MQ=255)
                                                                                        tCTGATCTCAACCGCGAGAAAAGAGCCTTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGCACCCCACATTGCGATTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCAt                                                <  1:2511193/140‑1 (MQ=255)
                                                                                        tCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTTATTTGGCCGGTAAAAAACGACTTCTCAGCCGGTGTGAATTAGAACCCCCCATTGCGTTTTTTTCCGTAGAATTTTTGCACCGGGCGCCAGAGCGTCAt                                                >  1:961112/1‑140 (MQ=255)
                                                                                        tCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCAt                                                >  2:919607/1‑140 (MQ=255)
                                                                                        tCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCAt                                                <  1:2642967/140‑1 (MQ=255)
                                                                                          tGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATga                                              <  1:3804259/140‑1 (MQ=255)
                                                                                          tGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATga                                              <  1:1649247/140‑1 (MQ=255)
                                                                                                    ggCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGAt                                    <  1:378160/140‑1 (MQ=255)
                                                                                                    ggCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGAt                                    <  2:3833544/140‑1 (MQ=255)
                                                                                                     gCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATc                                   <  1:2455350/140‑1 (MQ=255)
                                                                                                      cGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGGCAGGGAAAAAACCACTTCTCAGCCGCTATATAGTAGAACCCCACATTGCGGTTTTATCCGAAGAATTTGAGCAGCTGGCGAAAGAGCATCATGAGAACATGGATCa                                  >  2:597726/1‑140 (MQ=255)
                                                                                                      cGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGa                                                                                                       >  2:3350297/1‑71 (MQ=255)
                                                                                                      cGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGa                                                                                                       <  1:3350297/71‑1 (MQ=255)
                                                                                                         gaAAAGAGCCTTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGtt                               <  2:3667778/140‑1 (MQ=255)
                                                                                                         gaAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGtt                               <  2:619520/140‑1 (MQ=255)
                                                                                                          aaaaGAGCCGTTTTTTTATTTTTCATGTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTc                              <  2:3398846/140‑1 (MQ=255)
                                                                                                           aaaGAGCCGTTTTTTTATTTTTCATTTGCCCGGTAAAAAAAGACTTCTAAGCCGATATATAGTAGAACCCCACAATGCGTTTTTATCCGAAGAATTTTAGCACCTGGCGAAAGATCTTCATGTGAAAATGGAtaacttaa                             >  2:3481236/1‑133 (MQ=255)
                                                                                                               agCCGTTTTTTTATTTTTCATTTGCCATGTAAAAAACTACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACAtt                         <  1:1837349/140‑1 (MQ=255)
                                                                                                                gCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACAttt                        <  2:197799/140‑1 (MQ=255)
                                                                                                                         ttATTTTTTTTTTTCCAGGTAAAACTCGTATTCTCAGCCTATATATAGTAGAACCCCACTTTGCGTTTTTAGCCGAATAATTTGAGCAGCTGGCGAAATAGCTTCTTGAGAAGATGGATCAGTTCACATTTAAAATAAcc               <  1:189165/140‑1 (MQ=255)
                                                                                                                         ttATTTTTCATTTTCCAGGTAAAATACGACTTCTCTGCCGATATATAGTAGTACCCAACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAAca               <  2:1347569/140‑2 (MQ=255)
                                                                                                                         ttATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAAcc               <  1:2497895/140‑1 (MQ=255)
                                                                                                                           aTTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCa             <  1:1094606/140‑1 (MQ=255)
                                                                                                                           aTTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCa             <  1:3547749/140‑1 (MQ=255)
                                                                                                                              tttCATTTGCCAGTTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGt          <  2:2194836/140‑1 (MQ=255)
                                                                                                                              tttCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGt          <  2:2898237/140‑1 (MQ=255)
                                                                                                                              tttCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGt          <  1:2820072/140‑1 (MQ=255)
                                                                                                                              tttCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGt          <  1:160092/140‑1 (MQ=255)
                                                                                                                               ttCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTc         <  1:2174504/140‑1 (MQ=255)
                                                                                                                               ttCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTc         <  1:1422292/140‑1 (MQ=255)
                                                                                                                                tCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTcc        <  1:668670/140‑1 (MQ=255)
                                                                                                                                   tttGCCAGGTAAAAAACGACTTCTCTGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTCCAGa     <  1:4134749/140‑1 (MQ=255)
                                                                                                                                   tttGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTCCAGa     <  1:4194136/140‑1 (MQ=255)
                                                                                                                                    ttGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTCCAGAt    <  1:2711691/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                           
AAGGCGTTGGCCCCCATCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTCCAGATAG  >  NZ_CP034943/3268578‑3268851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: