Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,268,725 C→A 10.9% intergenic (+73/+137) catR → / ← EO946_RS16920 catDE operon transcriptional regulator CatR/HAMP domain‑containing histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,268,7250CA10.9% 582.4 / 13.6 232intergenic (+73/+137)catR/EO946_RS16920catDE operon transcriptional regulator CatR/HAMP domain‑containing histidine kinase
Reads supporting (aligned to +/- strand):  ref base C (35/162);  new base A (33/2);  total (68/164)
Fisher's exact test for biased strand distribution p-value = 9.30e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 234 total aligned reads displayed.

GGCCCCCATCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTCCAGATAGGACATGGGCT  >  NZ_CP034943/3268586‑3268861
                                                                                                                                           |                                                                                                                                        
ggCCCCCATCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAAc                                                                                                                                          >  2:1902013/1‑140 (MQ=255)
  cccccATCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGa                                                                                                                                        <  2:10639/140‑1 (MQ=255)
      cATCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTTCCAGGGAAAAAACGACtta                                                                                                                                    >  1:927076/1‑139 (MQ=255)
        tattGGTTGAGATTTCTACATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGATAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTtctc                                                                                                                                  <  2:1118454/138‑1 (MQ=255)
        tCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTtctc                                                                                                                                  <  1:1262795/140‑1 (MQ=255)
           tgtgTGAGATTTCTAAAGTGGCGACGGATTGGATTGACCCTTCCTTTGTTTACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                               <  1:3047670/137‑1 (MQ=255)
           tGGGTGAGATTTCTAAATGGGCGACGGTTTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                               <  1:3955563/140‑1 (MQ=255)
           tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCGTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                               <  1:1012573/140‑1 (MQ=255)
           tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAGACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                               <  2:2178816/140‑1 (MQ=255)
           tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                               <  2:1774873/140‑1 (MQ=255)
           tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                               <  2:956487/140‑1 (MQ=255)
           tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                               <  1:4332924/140‑1 (MQ=255)
           tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                               <  1:3388509/140‑1 (MQ=255)
           tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                               <  2:409664/140‑1 (MQ=255)
           tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                               <  1:221360/140‑1 (MQ=255)
           tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                               <  1:2998526/140‑1 (MQ=255)
           tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATGGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                               <  1:1189621/140‑1 (MQ=255)
           tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAAGTGCTAAAACGGTTCTGATCTCAAACGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                               <  2:1810248/140‑1 (MQ=255)
             ggTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTGATTTTCCAGGTAAAAAAAGACTTATGAGCCg                                                                                                                             >  1:3506148/1‑140 (MQ=255)
             ggTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCg                                                                                                                             <  1:906489/140‑1 (MQ=255)
             ggTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCg                                                                                                                             <  2:3480478/140‑1 (MQ=255)
             ggTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCg                                                                                                                             <  1:1983708/140‑1 (MQ=255)
             ggTGAGATTTCTAAATGGGCGACGGAGTGGATGGACCGTTCCTTTCTTGACTAATTCCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCg                                                                                                                             <  2:4324297/140‑1 (MQ=255)
             ggTGAGATTTCTAAAGGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCg                                                                                                                             <  1:4323157/140‑1 (MQ=255)
             ggTGAGATGTCAAAATGGGCGACGGATTGGCTGCACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCACAGGCGACAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCg                                                                                                                             <  1:250572/140‑1 (MQ=255)
              gTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGa                                                                                                                            <  2:3514908/140‑1 (MQ=255)
                tatttttCTATATGGGCGAGGTATTGTATTGACGCTTCATTTCTTGAATAATTTCTAAAACGGTTCTGATATCAAAGGCGATAAAAGAGCCGTTTTTTTATTTTTAATTTGCCAGGTAAAAAACGACTTCTCAGCCGata                                                                                                                          <  1:1500897/136‑1 (MQ=255)
                gagaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGata                                                                                                                          <  1:770112/140‑1 (MQ=255)
                gagaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGata                                                                                                                          <  2:2151050/140‑1 (MQ=255)
                gagaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGata                                                                                                                          <  2:1656943/140‑1 (MQ=255)
                gagaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGata                                                                                                                          <  2:1599612/140‑1 (MQ=255)
                gagaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGata                                                                                                                          <  1:830259/140‑1 (MQ=255)
                gagaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGata                                                                                                                          <  1:2153152/140‑1 (MQ=255)
                  gaTTTCTAACGGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAACCGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGatata                                                                                                                        <  1:1600813/140‑1 (MQ=255)
                  gaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGatata                                                                                                                        <  2:3227012/140‑1 (MQ=255)
                  gaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGatata                                                                                                                        <  1:3267024/140‑1 (MQ=255)
                   aTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTGTTTTTATTTTTGATTTTCCAGGTAAAAAAACACATCTAACCCGatatat                                                                                                                       >  1:2442520/1‑140 (MQ=255)
                     ttCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTCATAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATAtag                                                                                                                     <  1:2291031/140‑1 (MQ=255)
                       cTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATAtagta                                                                                                                   <  1:3168690/140‑1 (MQ=255)
                        tAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATAtagtag                                                                                                                  >  2:2455350/1‑140 (MQ=255)
                              ggCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAAcccc                                                                                                            >  2:3278522/1‑140 (MQ=255)
                              ggCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTAATTTGCCAGGTAAAAAAAGACTTATCACCCGATATAAAGTAGAAcccc                                                                                                            >  2:1422292/1‑140 (MQ=255)
                                cGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGGTAAAAAAGAATTATCAGCCGATATATATTAGAAcccccc                                                                                                          >  1:2965356/1‑138 (MQ=255)
                                   cGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACAtt                                                                                                       <  1:3802842/140‑1 (MQ=255)
                                          gattgACCCTTCCTTTCTTTACTAATTGCTAACACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGtttt                                                                                                <  1:3821444/140‑1 (MQ=255)
                                               aCCCTTCCTTTCTTGAGTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTTTTGTTGCTTTGCCAGGTAAAAAACGACGTCTCCGCCGCTATAAAGTGGAACCCCCCATTGCGTTTTTTGcc                                                                                           >  1:1219010/1‑140 (MQ=255)
                                               aCCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCATAGGCGAGAAAAGAGCCGTGTTTTTATTTTTCATTTTCCAGGTGAAAAAAGACTTCTCACCCCCTATATAGTAGAAACCCACAGAGGGTTTTTAggc                                                                                           >  1:4011643/1‑138 (MQ=255)
                                               aCCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTGCTTTGCCAGGTAAAAATCGAATTCTGAGCCGCTCTTTAGTATAACCCCCAATTGCGGGTTTAGcc                                                                                           >  1:4072021/1‑140 (MQ=255)
                                               aCCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGGCAGGTAAAAAAAGAATTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTTGcc                                                                                           >  2:323766/1‑140 (MQ=255)
                                               aCCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGcc                                                                                           >  2:636587/1‑140 (MQ=255)
                                               aCCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACAGTGCGTTTTTAGcc                                                                                           >  2:729532/1‑140 (MQ=255)
                                               aCCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAAAGAATTATAAGCCGATATATATTAGAACCCCACATTGCGTTTTTATcc                                                                                           >  2:272434/1‑140 (MQ=255)
                                               aCCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTGTTTTTATTTTTAATTTTGCAGGTAAAAAAACACTTGTCAGCCGATATATATTAGAACCCCACATTGCGTTTTTATcc                                                                                           >  1:562606/1‑140 (MQ=255)
                                                 ccttccttTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCTTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCga                                                                                         <  1:3754312/140‑1 (MQ=255)
                                                    tccttTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTTTTTTTTATTTTGCCGGTAAAAAAAACAATGTGAGCCGATATATAGTAGAACCCCCCAGTGGGTTTTTATCCggagg                                                                                      >  1:3616143/1‑139 (MQ=255)
                                                    tccttTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTTTTTTTTATTTTGAACGTTAAAAAAAACTTCTGGCCCGATTTATTGTTGAAACCCCCAATGGGTTTTTATGCgaaga                                                                                      >  1:1682014/1‑140 (MQ=255)
                                                    tccttTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTGATTTGGCAGGTAAAAAAAGACTTCTCAGCCGATATATAGTAGAACCCCACATTTCGTTTTTTTCCgaaga                                                                                      >  2:3754312/1‑140 (MQ=255)
                                                    tccttTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTGTTTTTATTTTTTATTTTCCCGGTAAAAAAAAACTTATCACCCGCTATATAGTATAACCCCACCTAGCCTTTTTATCCggaaa                                                                                      >  1:1246881/1‑136 (MQ=255)
                                                     ccttTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACATCTAAGCCCAAATATAGGAGAACCCCAACATGCGTATTTATCCgacgaa                                                                                     >  2:2315059/1‑140 (MQ=255)
                                                         tCTTGACTAATTGCTAAAACGGTTCTGATCACAAAGGCGAGAAAAGAGCCGTTTTTTTATTTATAAATTGCCAGGTAAAAAAAGACTTCTCAGCCGATATATAGTAGAAACCCACATTGCGTTTTTAGCCGAAGAAATTg                                                                                 >  2:3939022/1‑140 (MQ=255)
                                                              aCTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTTATTTTGCAGGTAAAAAAAGACATAGGAGCCCATATATATTATAAACCCCCAATGGGTGTTTATACGAACAATTTTatcag                                                                            >  1:601189/1‑140 (MQ=255)
                                                                  aTTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTAAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGACTGTGAGCAGCTgg                                                                        >  2:2255497/1‑140 (MQ=255)
                                                                             ggTTCTGATCTCAACGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCtt                                                             <  1:3081992/140‑1 (MQ=255)
                                                                             ggTTCTGATCTCAAAGGCGATAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCtt                                                             <  1:1365114/140‑1 (MQ=255)
                                                                               ttCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACCTTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCa                                                           <  2:293426/140‑1 (MQ=255)
                                                                               ttCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACAGTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCa                                                           <  2:2823105/140‑1 (MQ=255)
                                                                                tCTGATCTCAACGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCAt                                                          <  1:2538392/140‑1 (MQ=255)
                                                                                tCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTTATTTGGCCGGTAAAAAACGACTTCTCAGCCGGTGTGAATTAGAACCCCCCATTGCGTTTTTTTCCGTAGAATTTTTGCACCGGGCGCCAGAGCGTCAt                                                          >  1:961112/1‑140 (MQ=255)
                                                                                tCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCAt                                                          >  2:919607/1‑140 (MQ=255)
                                                                                       tccaaGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACAGTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGagaaga                                                   <  1:3022262/137‑1 (MQ=255)
                                                                                       tcAAAGGCTAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGagaaga                                                   <  1:1646961/140‑1 (MQ=255)
                                                                                       tcAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGagaaga                                                   <  2:1237488/140‑1 (MQ=255)
                                                                                            ggCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGAt                                              <  2:2679370/140‑1 (MQ=255)
                                                                                            cgCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGAt                                              <  1:2481902/139‑1 (MQ=255)
                                                                                              cGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGGCAGGGAAAAAACCACTTCTCAGCCGCTATATAGTAGAACCCCACATTGCGGTTTTATCCGAAGAATTTGAGCAGCTGGCGAAAGAGCATCATGAGAACATGGATCa                                            >  2:597726/1‑140 (MQ=255)
                                                                                              cGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGa                                                                                                                 >  2:3350297/1‑71 (MQ=255)
                                                                                              cGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGa                                                                                                                 <  1:3350297/71‑1 (MQ=255)
                                                                                                 gaAAAGAGCCTTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGtt                                         <  2:3667778/140‑1 (MQ=255)
                                                                                                 gaAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGtt                                         <  2:1881608/140‑1 (MQ=255)
                                                                                                  aaaaGAGCCGTTTTTTTATTTTTCATGTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTc                                        <  2:3398846/140‑1 (MQ=255)
                                                                                                       agCCGTTTTTTTATTTTTCATTTGCCATGTAAAAAACTACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACAtt                                   <  1:1837349/140‑1 (MQ=255)
                                                                                                        gCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACAttt                                  <  2:197799/140‑1 (MQ=255)
                                                                                                        gCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACAttt                                  <  2:4011643/140‑1 (MQ=255)
                                                                                                                 ttATTTTTTTTTTTCCAGGTAAAACTCGTATTCTCAGCCTATATATAGTAGAACCCCACTTTGCGTTTTTAGCCGAATAATTTGAGCAGCTGGCGAAATAGCTTCTTGAGAAGATGGATCAGTTCACATTTAAAATAAcc                         <  1:189165/140‑1 (MQ=255)
                                                                                                                 ttATTTTTCATTTTCCAGGTAAAATACGACTTCTCTGCCGATATATAGTAGTACCCAACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAAca                         <  2:1347569/140‑2 (MQ=255)
                                                                                                                   aTTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCa                       <  1:1094606/140‑1 (MQ=255)
                                                                                                                      tttCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGt                    <  2:2898237/140‑1 (MQ=255)
                                                                                                                      tttCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGt                    <  1:2820072/140‑1 (MQ=255)
                                                                                                                      tttCAAGTGCCAGCTAAAAAACGACTTCTCAGCCGATAGATAGTATAACCCCACATTGCCTTTTTAGCCGAAGAATTTGAGCAGCGCGCGAAAGAGCTGCATGAGAAGATGGAGCAGTTCACATTTAAAATAACCCACGt                    <  2:61103/140‑1 (MQ=255)
                                                                                                                       ttCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTc                   <  1:3278522/140‑1 (MQ=255)
                                                                                                                       ttCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTc                   <  1:2174504/140‑1 (MQ=255)
                                                                                                                       ttCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTc                   <  1:1952278/140‑1 (MQ=255)
                                                                                                                       ttCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTc                   <  1:1422292/140‑1 (MQ=255)
                                                                                                                        tCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTcc                  <  1:668670/140‑1 (MQ=255)
                                                                                                                           tttGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTCCAGa               <  1:4194136/140‑1 (MQ=255)
                                                                                                                           tgtGCCAGCTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCCGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTCCAGa               <  2:3034852/138‑1 (MQ=255)
                                                                                                                            ttGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTCCAGAt              <  1:2711691/140‑1 (MQ=255)
                                                                                                                                    tAAAAAACGACGTCTCAGCCGCTCTATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGACGATGGATAAGTTAACATTTAAAATAACCCACGTCCAGATAGGACATg      <  2:748494/140‑1 (MQ=255)
                                                                                                                                      aaaaaCGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTCCAGATAGGACAt       >  2:1444834/1‑137 (MQ=255)
                                                                                                                                        aaaCGACTTCTCAGCCGAGATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTCCAGATAGGACATGGGCt  <  1:2663400/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                        
GGCCCCCATCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTCCAGATAGGACATGGGCT  >  NZ_CP034943/3268586‑3268861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: