BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,280,312	C→A	5.3%	L136F (TTG→TTT)	EO946_RS16965 ←	lantibiotic dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,280,312	0	C	A	5.3%	884.2 / 7.5	319	L136F (TTG→TTT)	EO946_RS16965	lantibiotic dehydratase
Reads supporting (aligned to +/- strand): ref base C (225/67); new base A (2/24); total (227/92)
Fisher's exact test for biased strand distribution p-value = 1.56e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 325 total aligned reads displayed.

TCAAATAGAAAACAGATGTCGCACGAACACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTT  >  NZ_CP034943/3280173‑3280450

tCAAATAGAAAACAGATGTCGCACGAACACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTcc                                                                                                                                            >  2:1386535/1‑140 (MQ=255)

  aaaTAGAAAACAGATGTCGCACGAACACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCaa                                                                                                                                          >  2:1990908/1‑140 (MQ=255)

      aGAAAACAGATGTCGCACGAACACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAAtttt                                                                                                                                      <  2:4238127/140‑1 (MQ=255)

      aGAAAACAGATGTCGCACGAACACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAAtttt                                                                                                                                      <  1:1386535/140‑1 (MQ=255)

      aGAAAACAGATGTCGCACGAACACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAAtttt                                                                                                                                      <  2:3711730/140‑1 (MQ=255)

        aaacCAGATGTCGCTCGAACACTAACTTCGTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCt                                                                                                                                    <  2:3964135/140‑1 (MQ=255)

             aGATGTCGCACGAACACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCtaatta                                                                                                                               <  2:274947/140‑1 (MQ=255)

                 gTCGCACGAACACTAACTTCTTCAGATTTTCCATCTGTGCTGTCCAACAAGTAAGGCCGGTCACCCTTAATATAACAAGCGGACTTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTg                                                                                                                           >  1:2665808/1‑140 (MQ=255)

                  tCGCACGAACACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATGTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCCATTTTCTAATTAATTgg                                                                                                                          >  2:3339829/1‑140 (MQ=255)

                    gCACGAACACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTa                                                                                                                        >  1:3078141/1‑140 (MQ=255)

                    gCACGAACACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTa                                                                                                                        >  1:3032624/1‑140 (MQ=255)

                    gCACGAACACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAACTCGGTCGGTGTATTCGTTTTCCAATTTTCGAATTAATTGGTa                                                                                                                        >  1:3213110/1‑140 (MQ=255)

                            acTAACTGCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCGTAATATAACAAGCGGAATTTAATGTAAAAGACAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACt                                                                                                                <  2:1308971/140‑1 (MQ=255)

                                aCTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCaaa                                                                                                            >  2:3185818/1‑140 (MQ=255)

                                aCTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCaaa                                                                                                            >  1:100732/1‑140 (MQ=255)

                                aCTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCaaa                                                                                                            >  1:1244049/1‑140 (MQ=255)

                                              tCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCt                                                                                              >  1:4150771/1‑140 (MQ=255)

                                                    tgtgCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGt                                                                                        <  1:3709703/140‑1 (MQ=255)

                                                     gtgCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTGTGTa                                                                                       >  1:209890/1‑140 (MQ=255)

                                                           tACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAaatga                                                                                 >  1:1802237/1‑140 (MQ=255)

                                                                caaGTACGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGtt                                                                            >  1:67313/1‑140 (MQ=255)

                                                                caaGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGtt                                                                            >  1:333888/1‑140 (MQ=255)

                                                                caaGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGtt                                                                            >  2:923375/1‑140 (MQ=255)

                                                                caaGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGtt                                                                            >  2:2821000/1‑140 (MQ=255)

                                                                   gTACGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCTTTTTATTCGTTTTACAATTTTCTAATTAATTGTTAGAGCGACTCAAAATCCAAAAGAGCCATTTTGTAAAATGAATgatgat                                                                         >  2:1513320/1‑140 (MQ=255)

                                                                   gTAATCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGTTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGAt                                                                         >  2:2986338/1‑140 (MQ=255)

                                                                   gTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGt                                                                             >  1:925592/1‑136 (MQ=255)

                                                                   gTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGAt                                                                         <  2:1235035/140‑1 (MQ=255)

                                                                   gTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGAt                                                                         >  2:2574412/1‑140 (MQ=255)

                                                                   gTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGAt                                                                         >  2:4147013/1‑140 (MQ=255)

                                                                   gTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGAt                                                                         <  2:1401545/140‑1 (MQ=255)

                                                                   gTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGAt                                                                         >  1:2389159/1‑140 (MQ=255)

                                                                     aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCGTTGTTGTAAAATGAAGGGTGATTa                                                                       >  2:2553371/1‑140 (MQ=255)

                                                                     aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                       >  2:3440963/1‑140 (MQ=255)

                                                                     aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                       >  1:3850155/1‑140 (MQ=255)

                                                                     aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                       >  1:1847638/1‑140 (MQ=255)

                                                                     aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                       >  2:2145202/1‑140 (MQ=255)

                                                                     aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                       >  1:2655558/1‑140 (MQ=255)

                                                                     aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                       >  1:968571/1‑140 (MQ=255)

                                                                        cccGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTcaaa                                                                    >  1:603674/1‑140 (MQ=255)

                                                                        cccGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTaaaa                                                                    >  1:2311345/1‑140 (MQ=255)

                                                                            gTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATa                                                                >  1:3488352/1‑140 (MQ=255)

                                                                            gTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATa                                                                >  1:2193664/1‑140 (MQ=255)

                                                                                    tAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGAGAAGTGAttt                                                        >  1:2884577/1‑140 (MQ=255)

                                                                                        ataACAAGCGTAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTAt                                                    <  1:2051174/140‑1 (MQ=255)

                                                                                        ataACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTAt                                                    <  1:2022617/140‑1 (MQ=255)

                                                                                        agaaCACGCGGAATTTAATCCAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCAAATTAATTGGTAGAGCCACTAAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAACAGATAAGTGATTTTTAt                                                    <  1:2723905/138‑1 (MQ=255)

                                                                                           aCAAGCGGAATTTAATGTAAAAGAAGGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                 >  2:3276556/1‑140 (MQ=255)

                                                                                           aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                 >  1:733849/1‑140 (MQ=255)

                                                                                           aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                 >  2:3774994/1‑140 (MQ=255)

                                                                                           aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                 >  1:2485021/1‑140 (MQ=255)

                                                                                           aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                 >  2:2620125/1‑140 (MQ=255)

                                                                                           aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                 >  1:1677117/1‑140 (MQ=255)

                                                                                           aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCG‑TTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGa                                                >  1:707983/1‑140 (MQ=255)

                                                                                               tcttaatttaatttAAAACAAAGTCGGTCGGTGTATTCGTTTTCAATTTTTCTAATTAATTGTTAGAGCCACTCAAAATAAACACGAGCCTTTTTGTAAAATGAATGTTTATTAAAATATAAGTGTTTTTTATCAGaaaa                                             <  2:2234237/136‑1 (MQ=255)

                                                                                               gctgAATTTAATTTAAAAGAAAGTGGGTGGGTTTATTCGTTTTCAATTTTTCTAATTATTTGTTAGAGCAACTAAAAATAAACCCGAGCCTTTTTGTAAAATTAATTTTGATTAAAATATAAGTGATTTTTATCAGaaaa                                             <  2:2350192/137‑1 (MQ=255)

                                                                                               gctgAATTTAATGTAAAAGAAAGTCGGGCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACACAAAATCAACCCGAGCATTTTTGTAAAATGAATTTTGATTAAAAGATAAGTGTTTTTTATCAGaaaa                                             <  2:2682401/137‑1 (MQ=255)

                                                                                               gcgtattttATTTTAAAATAATGTGGGTGGGTTTTTTGGTTTTCAATTTTTCTAATTAATTGTTAGAGCCACTAAAAATCAACACGCGCCTTTTTTTAAAATGATTGTTTATTAAAAGATATGTGATTTTTATCAGaaaa                                             <  2:1816379/134‑1 (MQ=255)

                                                                                               gCGTAATTTAATTTAAAAGAAAGTCGGTCGGTTTATTCGTTTTCAAATTTTCTAATTAATTGGTAGAGCCACTAAAAATCAACACGAGCCTTTTTTTAAAATCAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                             <  2:2485127/140‑1 (MQ=255)

                                                                                               gCGTAATTTAATGTAAAAGAAAGTCGTTCTGTGTATTCTTTTTCCAATTTTCTAATTAATTGGTATAGCCACTAAAAATAAACACGAGCATTTTTTTAAAATGATTGTTGATTAAAATATAATTGATTTTTATAAGaaca                                             <  2:2383779/140‑3 (MQ=255)

                                                                                               gCGGAATTTAATTTAAACGAAAGTCGGCCGTTGTATTGTTTTTCAAATTTTCTATTTAATTGTTAGAGCCACTCAAAATAACCACCAGCCTTTTTGTAAAATGAATGTTTATTAAAAGATAAGTGATTTTTATAAGaaaa                                             <  2:585736/140‑1 (MQ=255)

                                                                                               gCGGAATTTAATGTAAACTAACGTCGGTCGGTGTTTTCGTTTTCCAATTTTCTAATTAATTGGTAGCGCAACTAAAAATAAACACGCGCCTTTTTGTAAAATGAATGTTGATTAAAATATACGTGTTTTTTATCAGaaaa                                             <  2:3985115/140‑1 (MQ=255)

                                                                                               gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAATTGATTTTTATCAGaaaa                                             >  2:3223997/1‑140 (MQ=255)

                                                                                               gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                             >  2:1865156/1‑140 (MQ=255)

                                                                                               gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                             >  2:559507/1‑140 (MQ=255)

                                                                                               gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                             >  2:2891825/1‑140 (MQ=255)

                                                                                               gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                             >  2:1660026/1‑140 (MQ=255)

                                                                                               gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                             >  2:730439/1‑140 (MQ=255)

                                                                                               gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATAAGaaaa                                             >  2:3816441/1‑140 (MQ=255)

                                                                                               ccttaatttaatttAAAACAATGTGGGGCGGTTTTTTCTTTTTCCATTTTTATATTTATTTGTTAGTGCAAATCAAAATCACCCCGAGCCTTTTTTTAAAATTAATTTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                             <  2:2444024/136‑1 (MQ=255)

                                                                                                 gtaaTTTAATGTAAAATAAAGTCGGTCGTTTTATTCGTTTTCCATTTTTATAATTAATTGGTAGAGCCACTCAAAAACAACACGAGCCTTTTTGTAAAATTAATGTTGATTAAAAGATAATTGATTTTTATCAGAAAACg                                           <  2:866924/138‑1 (MQ=255)

                                                                                                   aaTTTAATGTAAAATAAAGTCGGTCGGTGTATTCTTTTTCAAATTTTATAATTAATTGGTAGAGCAACTAAAAATAAACACGAGCATTTTTTTAAAATGAATGTTGATTAAAAGATACGTGATTTTTATCAGAAAACGAc                                         <  2:3032624/140‑1 (MQ=255)

                                                                                                          tGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGGAAAATGAATGTTGATTATGAGATAAGTGATTTTTTTGAGAAAACGACCCtatag                                  >  1:39864/1‑139 (MQ=255)

                                                                                                           ttAAAATAACGTCGGTGGTTGTATTCGTTTTCCAATTTTCTAATTAATTGTTAGACCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTTATTAAAATATAAGTGATTTTTATAAGAAAACGACCCTATAcc                                 <  2:407002/139‑1 (MQ=255)

                                                                                                           ttAAAAGAAAGTCGGTGGGTGTATTGTTTTTCCATTTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGCGCCTTTTTTTAAAATGAATGTTGATTAAAAGATACGTGATTTTTATCAGAAAACGACCCTATAcc                                 <  2:3596884/139‑1 (MQ=255)

                                                                                                             aaaaGAAATTCGTACGGTCTATACGTTTTAAAATTTTCTAATTAATTTATATAGAAAATAAAAATCAACACGAGCCTTTTTGTAAAATGAATTTTTATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATAACGa                               >  1:2952205/1‑140 (MQ=255)

                                                                                                             aaaaGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGa                               >  2:2092310/1‑140 (MQ=255)

                                                                                                             aaaaGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGa                               >  2:1052377/1‑140 (MQ=255)

                                                                                                             aaaaCAAAGTGGGTCGGTGTATTCGTTTTCCAATTTTCTAATTATTTGGTAGCGCCACTCAAAATCACCACGAGCATTTTTTTAAATTGAATGTTGATTAAAAGATACGTGATTTTTAACACAAAACGACCCTATACCGa                               <  2:1153684/140‑1 (MQ=255)

                                                                                                               aagaaagTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTATAGCCACTCAAAATCAACACGAGCGTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGAGTTTTATCAGAAAACGACCCGATAGCGACt                             >  1:2793316/1‑140 (MQ=255)

                                                                                                                    agTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTAt                                                    >  1:3778793/1‑112 (MQ=255)

                                                                                                                        ggtggttgtTTTCTTTTTCAATTTTTATATTTATTTGGTACAGCCAATAAAAATCAACACGAGCTTTTTTGTAAAATTAATTTTTATTAAAATATACGTTATTTTTATCAGAAAACGACCCTATACCGACTGAAGaaaaa                    <  1:4255459/134‑1 (MQ=255)

                                                                                                                              tttATTCTTTTTACAATTTTCTAATTATTTGTTAGAGCCACTAAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAATATAATTTATTTTTATAAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCa              <  2:295556/138‑1 (MQ=255)

                                                                                                                                tATTCTTTTTCAATTTTTCTAATTATTTGTTATACCAACAAAAATAAAACACTAGCTTTTTTTTAAATTTAATTTTTATTAAAATATATGTTATTTTTATCACAAAACGACCCTATACCGACTGAAGAAAAAAGGCCaaa            <  1:1219286/140‑1 (MQ=255)

                                                                                                                                   tCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAgg         >  1:2572795/1‑140 (MQ=255)

                                                                                                                                       tttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGtgtg     >  1:1183763/1‑140 (MQ=255)

                                                                                                                                        ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACTAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAAATACCCTATACCTACTGAAGAAAAAAGGCCAAAAGGTGTgg    >  1:2027442/1‑140 (MQ=255)

                                                                                                                                        ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAATGCCAAAAGGTGTgg    >  2:4107339/1‑140 (MQ=255)

                                                                                                                                        ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAACAGGTGTgg    >  2:3669265/1‑140 (MQ=255)

                                                                                                                                        ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg    >  2:2032989/1‑140 (MQ=255)

                                                                                                                                        ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg    >  1:237242/1‑140 (MQ=255)

                                                                                                                                        ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg    >  1:1868869/1‑140 (MQ=255)

                                                                                                                                        ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg    >  2:1919270/1‑140 (MQ=255)

                                                                                                                                        ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg    >  1:1749720/1‑140 (MQ=255)

                                                                                                                                        ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg    >  2:47318/1‑140 (MQ=255)

                                                                                                                                        ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg    >  1:439957/1‑140 (MQ=255)

                                                                                                                                        ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg    >  2:65802/1‑140 (MQ=255)

                                                                                                                                        ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg    >  1:3936063/1‑140 (MQ=255)

                                                                                                                                        ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAACGTGTgg    >  2:3866386/1‑140 (MQ=255)

                                                                                                                                        ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAAAGACCATATACCGACTGAAGAAAAAAGGCGAAAAGGTGTgg    >  2:2193569/1‑140 (MQ=255)

TCAAATAGAAAACAGATGTCGCACGAACACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTT  >  NZ_CP034943/3280173‑3280450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`