Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 438,810 G→T 6.0% intergenic (‑76/‑30) gabR ← / → gabT transcriptional regulator GabR/4‑aminobutyrate‑‑2‑oxoglutarate transaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943438,8100GT 6.0% 573.3 / 4.3 246intergenic (‑76/‑30)gabR/gabTtranscriptional regulator GabR/4‑aminobutyrate‑‑2‑oxoglutarate transaminase
Reads supporting (aligned to +/- strand):  ref base G (121/96);  new base T (4/22);  total (127/119)
Fisher's exact test for biased strand distribution p-value = 1.06e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 260 total aligned reads displayed.

ATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAACGGCAACCGCAGTCTGGCAGTCAAAGGAGT  >  NZ_CP034943/438672‑438949
                                                                                                                                          |                                                                                                                                           
aTAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGt                                                                                                                                            <  1:2903298/140‑1 (MQ=255)
        caggatagataAAAACGGATTATAATGAGAAGTAGAGTTAAAATTTGATCTACATTTGAAGATTCTCATTCTGGTACCATCAAAAAGTTATAATTGTTAATATTCATCAAACCAAAAAGAAGTAAATATGGTAAAAAAAt                                                                                                                                    >  2:4062259/4‑140 (MQ=255)
        caagatagataTAATATGATTGTTATAAGCAGTCGAGTGAGAATGTGATGTACATTTTCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATAACAAAAAGAAGTCAGAATGGTAATAAAAt                                                                                                                                    >  2:875175/4‑140 (MQ=255)
                atttaATCGGCTTGTTCTGAGGGGTGGAGTGTGAATGTGATGTCCTTTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGTTAAGAAAATAGCGACAg                                                                                                                            <  2:2407572/137‑1 (MQ=255)
                atatAATCGGTTTGTTCTGCGCGGTCGAGTGTGAATGTTATGTCAATTTGCATATTCTCCTTCTGGTACAAACAAAAAGTTATAATTGGTACTTTTCATCATACAAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAg                                                                                                                            <  2:2179900/140‑1 (MQ=255)
                  ataATCGGCTTGTTCTGAGCGGTCTAGTGTTATTGTGATGTCCATTTGCAGATTCTCCTTCTGGTACAATCAAAAAGTTATAATTGTTACTTTTAATCATACCAAAAAGAAGTCAGAATGTTAAGAAAATAGCGACAggg                                                                                                                          <  2:785137/140‑1 (MQ=255)
                    taTCGGCTGGTTGTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGAGTCTCCTTGTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACAAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAggggg                                                                                                                        <  2:3622868/139‑1 (MQ=255)
                    actCGGCTTGTTCTGCGCGGTCGTGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACAATCAAAAATTTATATTTGGTACTTTTCATCATACCAAAAAGAAGTCAGAAGGGTAATAAAATAGCGACAggggg                                                                                                                        <  2:1482870/138‑1 (MQ=255)
                      tCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGAc                                                                                                                      >  2:2315322/1‑140 (MQ=255)
                      tCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGAc                                                                                                                      >  1:3148189/1‑140 (MQ=255)
                      tCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGAc                                                                                                                      >  1:3264922/1‑140 (MQ=255)
                       gggCTTTTTCTGAGCGGTCGTGTGTTAATGTGATTTCATTTTGCATATTCTCCTTCTGTTACCATCAAAAATTTATAATTGGTACTTTTCATCATACAAAAAAGAAGTCATAATGGTAAGAAAATAGCGACAGGGGGACa                                                                                                                     <  2:535168/139‑1 (MQ=255)
                       gggCTGGTGCTGCGCGGTCGAGTGTGAATGTGATGGCCATTTGCACATTCTCCTTCGGGTACCATCAAAAAGTTATAATTGGAACTTTTCATCATACCAAAAAGAAGTCACAATGGTAAGAAAATAGCGACAGGGGGACa                                                                                                                     <  2:2224768/139‑1 (MQ=255)
                       cGGCTTGTTCTCAGGGGTCGTGTGTTATTGTGATGTCCATTTGCAGTTTCTCCTTCTGGTACCATCAAAAAGTTTTAATGGGTACTTTTCATCATACCAAAAAGAAGTCAGAAGGGTAAGAAAATAGCGACAGGGGGACa                                                                                                                     <  2:251264/140‑1 (MQ=255)
                         gCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATa                                                                                                                   >  2:2870061/1‑140 (MQ=255)
                          cTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCAGTTGCAGATTCTCATTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTAATCATAACAAAAACAAGTCAGAATGGTAAGAAAATCTCGACAGGGGGACCTAc                                                                                                                  >  2:3272806/1‑140 (MQ=255)
                           ttGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACg                                                                                                                 >  1:3829512/1‑140 (MQ=255)
                           ttGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACg                                                                                                                 >  1:1079759/1‑140 (MQ=255)
                            tGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCATATTCTCCTTCTGGTACCAACAAAAAGTTATAATTGGTAGTTTTCATCATACCAAAAATAAGTCACATTGGTAAGAAAATAGCGACAGGGGGACATACGc                                                                                                                <  2:510460/140‑1 (MQ=255)
                            tGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATActc                                                                                                                >  2:1041602/1‑138 (MQ=255)
                            tGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGc                                                                                                                >  2:4183403/1‑140 (MQ=255)
                             gTTCTGAGCGTTCGAGTGTGATTGTGATGTCATTTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTAATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCa                                                                                                               <  2:2362937/140‑1 (MQ=255)
                              ttCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCAt                                                                                                              >  2:3130064/1‑140 (MQ=255)
                                  gAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGt                                                                                                          >  1:1377666/1‑140 (MQ=255)
                                   aGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACCTACGCATGAGTc                                                                                                         >  2:1181966/1‑140 (MQ=255)
                                   aGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTc                                                                                                         >  2:3694727/1‑140 (MQ=255)
                                   aGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTc                                                                                                         >  2:1216170/1‑140 (MQ=255)
                                   aGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTc                                                                                                         >  1:3296546/1‑140 (MQ=255)
                                   aGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTc                                                                                                         >  1:1781202/1‑140 (MQ=255)
                                   aGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTc                                                                                                         >  1:1011961/1‑140 (MQ=255)
                                    gctgTCGTGTTTGAATGTGTTGCACATTTGCCTATTCTCCTTCTGGTACAACCAAAATGTTTTAATTGTTACTTTTCATCATACCAAAATTAAGAAAGATTGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCa                                                                                                        <  2:696906/137‑1 (MQ=255)
                                      gggcGAGTGTGAATGTTATTTCCATTGGCACATTCACCTTCTGGAACCATCAAAAAGTTTTACTTGCTACTTTTCATAATACAAAAAATAAGTCAGAAGGGTATGAAAAAAGCGACAGGGGGACATACGCATGAGTCaaa                                                                                                      <  2:3034362/137‑1 (MQ=255)
                                       gTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAaac                                                                                                     >  1:500272/1‑140 (MQ=255)
                                        tCGAGTGTGACTGTGATGTCCATGTGCAGATTCTCCTTCTGGTACCAGCAAAAAGTGATCATTGGTACTTTTCATCATACCAAAAAGAAGTCCGAATGGTCAGAAAATAGCGACAGGGGGACATACGCATGAGTCAaaca                                                                                                    >  1:2061368/1‑140 (MQ=255)
                                        tCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAaaca                                                                                                    >  2:166386/1‑140 (MQ=255)
                                        tCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAaaca                                                                                                    >  2:4128394/1‑140 (MQ=255)
                                        tCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAaaca                                                                                                    >  2:3943909/1‑140 (MQ=255)
                                        tCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAaaca                                                                                                    >  2:3562438/1‑140 (MQ=255)
                                        tCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAaaca                                                                                                    >  2:977631/1‑140 (MQ=255)
                                        tCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAaaca                                                                                                    >  1:3607833/1‑140 (MQ=255)
                                        tCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAaaca                                                                                                    >  1:3540420/1‑140 (MQ=255)
                                        tCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAaaca                                                                                                    >  1:1643899/1‑140 (MQ=255)
                                        aacattgtcAATGTGATGTAAATAAGAATAATCTACTTATGGTACCATCAAAAAGTTATAATTGGTACTTTTCATAATACAAAAAAGAAGTCAGAATGGTAAGAAAATAGAGACAGGTGAAAATAATCATTATTCaaaaa                                                                                                    >  2:2359400/6‑138 (MQ=255)
                                             tgtgAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGACGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACagc                                                                                               >  1:465961/1‑140 (MQ=255)
                                              ttcattgtgATGACCATTTCACTTTTCTCCTTCTGGTACCAAAAAAAATTTATAATTGGAACTTTAAATCATACCAAAAATAAGACAGATTGTTAACAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACagca                                                                                              <  1:2214942/135‑1 (MQ=255)
                                              gttaaTTTGATGTCCTTTTGCTTTTTCTACTTCTGTTAACATAAAAAATTTATAATTGGTACTTTTCATCATACCAAAAATAAGTCAGAATGGTAATAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACagca                                                                                              <  2:2157142/137‑1 (MQ=255)
                                                  aTGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACagcaagca                                                                                          >  2:2744/1‑140 (MQ=255)
                                                  aTGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACagcaagca                                                                                          >  1:2769776/1‑140 (MQ=255)
                                                  aTGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACagcaagca                                                                                          >  2:1921963/1‑140 (MQ=255)
                                                   tgtgATGTCCATTTGCAGATTCTCCTTCTGGTACCATAAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCAt                                                                                         <  2:4037375/140‑1 (MQ=255)
                                                      gATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCAc                                                                                      >  2:4279985/1‑140 (MQ=255)
                                                       aTGTCCATTTGCAGATTCTCCTTCCGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAATTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACg                                                                                     >  2:2901731/1‑140 (MQ=255)
                                                        tGTCCATTTGCATTTTCACATTCTGGAACAAAAAAAACTTTATATTGGTTAATTTCCAACATACAAAAAACAAGACACAATGGAACCAAAACAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGa                                                                                    <  1:1209961/140‑1 (MQ=255)
                                                        tGTCCATTTGCAGATTCTCCTTCTGGTACAATCAAAAAGTTATAATTGGTACTTTTCATAATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGa                                                                                    <  2:809246/140‑1 (MQ=255)
                                                        gttCCATTGCCAGATTCCCCTTCTGGAACAACCAAAACGTTAAACTTGGTACTTTTCAACAAACAAAAAACAAGTCAGATTGGTAACAAAAGCGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGa                                                                                    <  1:2275596/138‑1 (MQ=255)
                                                         gTCCTTTTGCAGATACTCCTACTGGTACAATCAAAAATTTATAATTGGTACTTTTCATCATACAAAAAACAAGTCACAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGAc                                                                                   <  1:408680/140‑1 (MQ=255)
                                                         gTCCATTTGCAGATACTACTTCTGGAACCAACAAAAAGTTATAATTGTTACTTTACATCATACAAAAAAGAAGTAAGAATGCTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGAc                                                                                   <  1:4222113/140‑1 (MQ=255)
                                                              tttGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGccc                                                                              >  2:1724303/1‑140 (MQ=255)
                                                                  cAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTg                                                                          >  2:1441406/1‑140 (MQ=255)
                                                                    gATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGgc                                                                        >  1:2987211/1‑140 (MQ=255)
                                                                       tctcCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGgcagc                                                                     >  2:837745/1‑140 (MQ=255)
                                                                          ccTTCTGGAACCAAAAAAAATTTATAATTGGTACTTTTCAACATACAAAAAATATGTCAGATTGTTAACAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCaaa                                                                  <  1:2179905/140‑1 (MQ=255)
                                                                                 gTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGa                                                           >  1:1202628/1‑140 (MQ=255)
                                                                                   aCCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGc                                                         >  2:612942/1‑140 (MQ=255)
                                                                                       tCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGTATGGGAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCACtt                                                     >  2:2204633/1‑140 (MQ=255)
                                                                                       tCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCCACACCGGGAGCCAtt                                                     >  2:2985861/1‑140 (MQ=255)
                                                                                       tCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAAtt                                                     >  2:262856/1‑140 (MQ=255)
                                                                                       tCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGGCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAAtt                                                     >  2:2089045/1‑140 (MQ=255)
                                                                                        caacaaGTTATAATTGCCACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAACAACGGGAGCAATTc                                                    <  2:2706263/140‑1 (MQ=255)
                                                                                        cAAAAAGTTATAATTGGTACTTTTCATCATACAAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTc                                                    <  2:722536/140‑1 (MQ=255)
                                                                                        aaaaaaGTTATAATTGTTACTTTTAATCATACCAAAAAGAACTCAGAATGGTAATAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTc                                                    <  2:1535471/139‑1 (MQ=255)
                                                                                          aaaaGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGTTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGt                                                  <  1:2896068/140‑1 (MQ=255)
                                                                                              gTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGACAATAGCGACATGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACCGGAGCAATACGTCTcc                                              >  2:3613062/1‑140 (MQ=255)
                                                                                                tataATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCACCAAAAACGGGAGCACTTCGTCTCCaa                                            >  1:887414/1‑140 (MQ=255)
                                                                                                 ataATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCaaa                                           >  1:613662/1‑140 (MQ=255)
                                                                                                           tttttAATCATAAAAAAAATATTTCTTATTGTTAATAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCa                                 <  1:1497213/139‑1 (MQ=255)
                                                                                                             tttCATCCTCCCGAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAAc                               <  2:215574/140‑1 (MQ=255)
                                                                                                             tttCATCATACCAAAAATAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAAc                               <  1:2374204/140‑1 (MQ=255)
                                                                                                             tttCATCATACCAAAAAGTAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAAc                               <  2:3376426/140‑1 (MQ=255)
                                                                                                             tttCATCATACCAAAAAGAAGTCAGAATGGAAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAAc                               <  2:1021564/140‑1 (MQ=255)
                                                                                                              ttCATCATACCAAAAAGCAGTCAGAAGGGTAACAAAATAGCGACAGGGGGACATACGCATGCGTCAAACAACAGCAATCAGCACGACTGGCGAGTCGCAGCAAAAACGGGAGCAATACGTCTCCCAAGGTGTGCGCACCg                              >  1:2810598/1‑140 (MQ=255)
                                                                                                              ttCATAATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAAAAGCAAGCATCACTACTGACCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAACg                              >  1:2643189/1‑140 (MQ=255)
                                                                                                                   catACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTc                                                    >  1:3096232/1‑113 (MQ=255)
                                                                                                                     tACCAAACCGAAGTCAGCAGGGTAACAAATTAGCGACAGGGGGACATACGCATGAGTCAAACCACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAACCGGGAGCAATTCGTCTCCAAAGGTGTGAGCAACTGCAACCg                       <  1:2129290/140‑1 (MQ=255)
                                                                                                                     tACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAACGGCAACCg                       <  2:1202558/140‑1 (MQ=255)
                                                                                                                      aCCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAACGGCAACCGc                      >  2:3436510/1‑140 (MQ=255)
                                                                                                                        cAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAACGGCAACCGCAg                    >  2:1687902/1‑140 (MQ=255)
                                                                                                                              caagTCATAAGGGTAATAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAACGGCAACCGCAGTCTGGc              <  1:250345/139‑1 (MQ=255)
                                                                                                                               aagTAAGAATGTTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAACGGCAACCGCAGTCTGGCa             <  2:3220227/140‑1 (MQ=255)
                                                                                                                                  tCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAACGGCAACCGCAGTCTGGCAGTCa         <  2:1584198/140‑1 (MQ=255)
                                                                                                                                   cataaTGTTAACAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAACGGCAACCGCAGTCTGGCAGTCa         <  2:2598360/137‑1 (MQ=255)
                                                                                                                                    aGACTGGTAACAAAACAGCGACAGGGGCACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAACGGCAACCGCAGTCTGGCAGTCaa        <  1:1441651/140‑1 (MQ=255)
                                                                                                                                    aGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTAAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAACGGCAACCGCAGTCTGGCAGTCaa        <  2:2306887/140‑1 (MQ=255)
                                                                                                                                     gattGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAACGGCAACCGCAGTCTGGCAGTCaaa       <  1:1687902/137‑1 (MQ=255)
                                                                                                                                     gAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAACGGCAACCGCAGTCTGGCAGTCaaa       <  1:3908268/140‑1 (MQ=255)
                                                                                                                                      aaTGGTAAGAAAATAGCGACACGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCACCGGCAACCGCAGTCTGGCAGTCAAAg      >  1:3732275/1‑140 (MQ=255)
                                                                                                                                          gTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAGACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAACGGCAACCGCAGTCTGGCAGTCAAAGGAGt  <  2:1425744/140‑1 (MQ=255)
                                                                                                                                          gTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAACGGCAACCGCAGTCTGGCAGTCAAAGGAGt  <  1:4279985/140‑1 (MQ=255)
                                                                                                                                          gTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAACGGCAACCGCAGTCTGGCAGTCAAAGGAGt  <  1:3943909/140‑1 (MQ=255)
                                                                                                                                          gTAAGAAAAGAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAACGGCAACCGCAGTCTGGCAGTCAAAGGAGt  <  2:3742535/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
ATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAACGGCAACCGCAGTCTGGCAGTCAAAGGAGT  >  NZ_CP034943/438672‑438949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: