BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,280,341	C→A	8.8%	E127* (GAG→TAG)	EO946_RS16965 ←	lantibiotic dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,280,341	0	C	A	8.8%	882.9 / 25.4	333	E127* (GAG→TAG)	EO946_RS16965	lantibiotic dehydratase
Reads supporting (aligned to +/- strand): ref base C (235/58); new base A (2/38); total (237/96)
Fisher's exact test for biased strand distribution p-value = 2.71e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 334 total aligned reads displayed.

ACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAA  >  NZ_CP034943/3280205‑3280480

aCTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAGTTTGCTAATTAATTGGTAGAGCCACTCaaa                                                                                                                                          >  2:3025782/1‑140 (MQ=255)

aCTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCaaa                                                                                                                                          >  1:980688/1‑140 (MQ=255)

aCTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCaaa                                                                                                                                          >  2:3979622/1‑140 (MQ=255)

    cttcAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCa                                                                                                                                      >  2:2933362/1‑140 (MQ=255)

      tcAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAac                                                                                                                                    >  1:1549231/1‑140 (MQ=255)

            tttCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAATCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCAAATTTTATAATTAATTGGTAGAGCCAGTCAAAATCAACACCAGc                                                                                                                              >  2:2979886/1‑140 (MQ=255)

            tttCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGc                                                                                                                              >  1:3424675/1‑140 (MQ=255)

            tttCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGc                                                                                                                              >  1:3932962/1‑140 (MQ=255)

            ttcccATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGc                                                                                                                              >  2:3672548/4‑140 (MQ=255)

              tCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGTGCCACTCAAAATCAACACGAGCCt                                                                                                                            >  2:3412192/1‑140 (MQ=255)

              tCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCt                                                                                                                            >  1:4150771/1‑140 (MQ=255)

                    tgtgCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGt                                                                                                                      <  1:3709703/140‑1 (MQ=255)

                    tgtgCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGt                                                                                                                      <  1:2352673/140‑1 (MQ=255)

                     gtgCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTa                                                                                                                     >  1:2432881/1‑140 (MQ=255)

                     gtgCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTGTGTa                                                                                                                     >  1:209890/1‑140 (MQ=255)

                        cTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTaaaa                                                                                                                  >  2:45178/1‑140 (MQ=255)

                             caacaaGTAAGCCCGGTCACCCTTAATATAACACGCGGAATTTAATGTACAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTCATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAaatgaat                                                                                                             >  1:2798254/1‑140 (MQ=255)

                               acaaGTAAGCCCGGTCACCCTTAATATATCAAGCGGAATTGAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGCTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGt                                                                                                           <  1:3056391/140‑1 (MQ=255)

                                caaGTACGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGtt                                                                                                          >  1:67313/1‑140 (MQ=255)

                                caaGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTCATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGtg                                                                                                          >  1:3514934/1‑139 (MQ=255)

                                caaGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGtt                                                                                                          >  2:2583324/1‑140 (MQ=255)

                                caaGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGtt                                                                                                          >  2:923375/1‑140 (MQ=255)

                                   gTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGAt                                                                                                       >  1:3807588/1‑140 (MQ=255)

                                   gTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGAt                                                                                                       <  2:1235035/140‑1 (MQ=255)

                                   gTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGAt                                                                                                       <  2:1582264/140‑1 (MQ=255)

                                     aaGCCCGGTCACCCTTAATCTAACAAGCGGAATTTAATGTACAAGAAAGTCGGTCGGTGTCTTCGTTTTCCAATTTTCGAATTAATTGGTAGAGCCACTCAAAATCAACTCGAGCGTTTTTGTAAAATGAATGTTTAtga                                                                                                     >  2:3580921/1‑138 (MQ=255)

                                     aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                     >  1:3193204/1‑140 (MQ=255)

                                     aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                     <  2:2127919/140‑1 (MQ=255)

                                     aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                     >  1:1847638/1‑140 (MQ=255)

                                     aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                     >  1:2485127/1‑140 (MQ=255)

                                     aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                     <  1:2574412/140‑1 (MQ=255)

                                     aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                     >  1:265691/1‑140 (MQ=255)

                                     aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                     >  1:2749148/1‑140 (MQ=255)

                                        cccGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTcaaa                                                                                                  >  1:603674/1‑140 (MQ=255)

                                            gttaCCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATa                                                                                              >  1:1812915/4‑140 (MQ=255)

                                            gTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTACAAGGAATGTTGATTAAAAGATa                                                                                              >  2:3826807/1‑140 (MQ=255)

                                            gTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATa                                                                                              >  1:2193664/1‑140 (MQ=255)

                                                    tAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGAGAAGTGAttt                                                                                      >  1:2884577/1‑140 (MQ=255)

                                                     aaTATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGTTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGAtttt                                                                                     >  1:1346579/1‑140 (MQ=255)

                                                        ataACAAGCGTAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTAt                                                                                  <  1:2051174/140‑1 (MQ=255)

                                                        ataACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTAt                                                                                  <  1:2022617/140‑1 (MQ=255)

                                                          aaCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCa                                                                                <  2:2479864/140‑1 (MQ=255)

                                                           aCAAGCGGACTTTAATGTAAAAGAAAGTCGGTCGGTGTCTTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGCTGACAAGATAAGTGATTTTTATCAg                                                                               >  2:2389954/1‑140 (MQ=255)

                                                           aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCat                                                                               >  2:1298706/1‑139 (MQ=255)

                                                           aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                               >  1:733849/1‑140 (MQ=255)

                                                           aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                               >  2:3778394/1‑140 (MQ=255)

                                                           aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                               >  2:4110943/1‑140 (MQ=255)

                                                           aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATAAg                                                                               >  2:3288166/1‑140 (MQ=255)

                                                           aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGAGTAAAAGAGAAGTGATTTTTATCAg                                                                               >  2:3194490/1‑140 (MQ=255)

                                                           aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCG‑TTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGa                                                                              >  1:707983/1‑140 (MQ=255)

                                                           aCAAGCGGAATATAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACCAGCCTTTTTGTAAAATGAATGTTTATTAAAAGATAAGTGATTTTTATCat                                                                               >  2:30861/1‑139 (MQ=255)

                                                               tcttaatttaatttAAAACAAAGTCGGTCGGTGTATTCGTTTTCAATTTTTCTAATTAATTGTTAGAGCCACTCAAAATAAACACGAGCCTTTTTGTAAAATGAATGTTTATTAAAATATAAGTGTTTTTTATCAGaaaa                                                                           <  2:2234237/136‑1 (MQ=255)

                                                               gcttaaTTTACTGTAAAATAAATTGGTTCGGTTTTTTCTTTTTCCAATTTTATAATTATTTGGTAGAGCCACTCAAAATCAACCCGTGCCTTTTTGTAAAATGAATGTTGATTAAAAGTTATGTGATTTTTATCAGaaaa                                                                           <  2:1387683/136‑1 (MQ=255)

                                                               gctgAATTTAATTTAAAAGAAAGTGGGTGGGTTTATTCGTTTTCAATTTTTCTAATTATTTGTTAGAGCAACTAAAAATAAACCCGAGCCTTTTTGTAAAATTAATTTTGATTAAAATATAAGTGATTTTTATCAGaaaa                                                                           <  2:2350192/137‑1 (MQ=255)

                                                               gctgAATTTAATGTAAAAGAAAGTCGGGCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACACAAAATCAACCCGAGCATTTTTGTAAAATGAATTTTGATTAAAAGATAAGTGTTTTTTATCAGaaaa                                                                           <  2:2682401/137‑1 (MQ=255)

                                                               gctcaatttaatttAAAATAAAGTCGTTCGTTTTATTCGTTTTCAATTTTTCTATTTATTTGTTAGAGCCACTAAAAATCAACACTAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTTATTTTTATCAGaaaa                                                                           <  2:288588/136‑1 (MQ=255)

                                                               gcgtattttATTTTAAAATAATGTGGGTGGGTTTTTTGGTTTTCAATTTTTCTAATTAATTGTTAGAGCCACTAAAAATCAACACGCGCCTTTTTTTAAAATGATTGTTTATTAAAAGATATGTGATTTTTATCAGaaaa                                                                           <  2:1816379/134‑1 (MQ=255)

                                                               gCGTAATTTATTGTAAAACAAAGTCGGTCGGTTTATTCTTTTTCAAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATTAATGTTGATTAAAAGATATGTGATTTTTATCATaaaa                                                                           <  2:568893/140‑1 (MQ=255)

                                                               gCGTAATTTAATTTAAAATAACTTGGGTGGGTTTATTCGTTTTCAAATTTTCTAATTATTTGTTAGAGCCACTCAAAATAAACACGAGCCTTTTTGTAAAATGATTGTTGATTAAAAGATACGTGATTTTTATAAGaaaa                                                                           <  2:2798254/140‑1 (MQ=255)

                                                               gCGGAATTTAATTTAAAAGAACTTCGGTGCGTGTATTCTTTTTCCATTTTTCTAATTAATTGGTAGAGCAACTCAAAATCAACACGAGCCTTTTTTTAAATTTAATTTTTATTAAAAGATAAGTGTTTTTTATCATaaaa                                                                           <  2:203436/140‑1 (MQ=255)

                                                               gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTGTACTTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                                                           >  2:2214094/1‑140 (MQ=255)

                                                               gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                                                           >  2:2156674/1‑140 (MQ=255)

                                                               gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                                                           >  2:1660026/1‑140 (MQ=255)

                                                               gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                                                           >  2:559507/1‑140 (MQ=255)

                                                               gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                                                           >  2:730439/1‑140 (MQ=255)

                                                               ccgtaatttaatttAAAAGATAGTCGGTCGGTTTATTCGTTTTAAAATTTTCTAATTAATTGGTATAGCCAAAAAAAATAAACACGAGCATTTTTTTAAAATGATTGTTTATAAAAAGATAAGTGATTTTTATCACaaca                                                                           <  2:408584/136‑3 (MQ=255)

                                                                 gtaaTTTAATGTAAAATAAAGTCGGTCGTTTTATTCGTTTTCCATTTTTATAATTAATTGGTAGAGCCACTCAAAAACAACACGAGCCTTTTTGTAAAATTAATGTTGATTAAAAGATAATTGATTTTTATCAGAAAACg                                                                         <  2:866924/138‑1 (MQ=255)

                                                                          tGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGGAAAATGAATGTTGATTATGAGATAAGTGATTTTTTTGAGAAAACGACCCtatag                                                                >  1:39864/1‑139 (MQ=255)

                                                                             aaaaGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGa                                                             >  2:1052377/1‑140 (MQ=255)

                                                                             aaaaGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGa                                                             >  2:1451597/1‑140 (MQ=255)

                                                                             aaaaGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATAGGTAGAGCAACTCAAAATCAACACGAGCCTTTTTCTAAAATGAATGTTAATTAAAATATAAGTGATAATAATCAGAAAACGACACTATACAGa                                                             >  2:4060308/1‑140 (MQ=255)

                                                                             aaaaCAAAGTGGGTCGGTGTATTCGTTTTCCAATTTTCTAATTATTTGGTAGCGCCACTCAAAATCACCACGAGCATTTTTTTAAATTGAATGTTGATTAAAAGATACGTGATTTTTAACACAAAACGACCCTATACCGa                                                             <  2:1153684/140‑1 (MQ=255)

                                                                                        gttcggtTTATTCTTTTTAAAATTTTATAATTAATTGGTAGAGCAACTAAAAATCAACACCAGCCTTTTTGTAAAATGAATGTTTATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGaaaaa                                                  <  2:645879/138‑1 (MQ=255)

                                                                                        ggtggttgtTTTCTTTTTCAATTTTTATATTTATTTGGTACAGCCAATAAAAATCAACACGAGCTTTTTTGTAAAATTAATTTTTATTAAAATATACGTTATTTTTATCAGAAAACGACCCTATACCGACTGAAGaaaaa                                                  <  1:4255459/134‑1 (MQ=255)

                                                                                                tATTCTTTTTCAATTTTAGTAATTATTTGTTCGAGACACACAAACACAACACGCTCCTTTTGTTAAAATCAATTTTTATTAAAAGATATGTTTTTTTTATAATAACACGACCCTATACCGACTGAAGAAAAAAGGCCaaa                                          <  1:1228207/140‑1 (MQ=255)

                                                                                                   tCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAgg                                       >  1:2957084/1‑140 (MQ=255)

                                                                                                    ctttttCCATTTTTTTAATTAATTGGTAGAGCCCAAAAAAATCAACACGAGCTTTTTTTTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGgt                                      <  2:3258868/138‑1 (MQ=255)

                                                                                                       tttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGATGAAAAAAGGCCAAAAGGtgtg                                   >  1:2381504/1‑140 (MQ=255)

                                                                                                       tttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGtgtg                                   >  1:1183763/1‑140 (MQ=255)

                                                                                                        ttCCAATTTTCTAATTAATTGGTATAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                  >  1:408986/1‑140 (MQ=255)

                                                                                                        ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACTAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAAATACCCTATACCTACTGAAGAAAAAAGGCCAAAAGGTGTgg                                  >  1:2027442/1‑140 (MQ=255)

                                                                                                        ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGAGCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                  >  1:1766747/1‑140 (MQ=255)

                                                                                                        ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                  >  1:439957/1‑140 (MQ=255)

                                                                                                        ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                  >  2:3882959/1‑140 (MQ=255)

                                                                                                        ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                  >  1:3753009/1‑140 (MQ=255)

                                                                                                        ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                  >  2:1919270/1‑140 (MQ=255)

                                                                                                        ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                  >  2:3748261/1‑140 (MQ=255)

                                                                                                        ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                  >  2:198632/1‑140 (MQ=255)

                                                                                                        ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                  >  1:1868869/1‑140 (MQ=255)

                                                                                                        ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCGATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                  >  1:940668/1‑140 (MQ=255)

                                                                                                          ccAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATTTTGATTAAAAGATAAGTGATTTTTATCAGATAACGACCCTATACCGACTCAATAAAAAAGGCCAAATGGTGTGGtt                                >  2:4230518/1‑140 (MQ=255)

                                                                                                                 tCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGTTTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCttgttg                         >  1:1004840/1‑140 (MQ=255)

                                                                                                                             ttggagCCACTCAAACTCAACACGAGCCTTTTTGTAAAATGAATTTTTATTAAAACATATGGGATTTTTATCAGAAAACGACCCTATCACGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCttgttg             <  2:660608/137‑1 (MQ=255)

                                                                                                                               agagCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa           >  2:1875966/1‑140 (MQ=255)

                                                                                                                               agagCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa           >  2:2565092/1‑140 (MQ=255)

                                                                                                                               agacCCACACAAAATCAACACGAGCCTTTTTGAAAAATGATTTTTGATTAATAGTTACTTGATTTTTTTCAGAAAACGACCCGATCCCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa           <  1:1012918/140‑1 (MQ=255)

                                                                                                                                   ccACTCAAAATCCACACGAGCCTTTTTGTAAACTGATTGTTGATAAAAAGATCAGTGATTTTTAGCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACt       <  2:2485620/140‑1 (MQ=255)

                                                                                                                                   ccACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACt       >  2:251360/1‑140 (MQ=255)

                                                                                                                                   ccACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACt       >  2:2651120/1‑140 (MQ=255)

                                                                                                                                        cAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCaaa  >  2:3611960/1‑140 (MQ=255)

ACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAA  >  NZ_CP034943/3280205‑3280480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`