BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,285,647	C→A	39.2%	V252L (GTA→TTA)	EO946_RS16995 ←	IS3 family transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,285,647	0	C	A	39.2%	659.5 / inf	751	V252L (GTA→TTA)	EO946_RS16995	IS3 family transposase
Reads supporting (aligned to +/- strand): ref base C (268/187); new base A (180/114); total (449/302)
Fisher's exact test for biased strand distribution p-value = 5.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 758 total aligned reads displayed.

TAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGCCCAT  >  NZ_CP034943/3285508‑3285784

tAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAc                                                                                                                                            <  2:2217769/140‑1 (MQ=40)

tAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAc                                                                                                                                            <  1:2922847/140‑1 (MQ=40)

     tGTATACACAGAACCTTGGTCACTCTGTAACACGCAGTTCTCATGCAGTGTTGGCATCTGATTAAGTATGTCTAAGAAAAAGTCTGTTTACTGCTTATCCCCAATCGTAAAAGCTATCACTTATCCATTGTATAAATCTa                                                                                                                                       <  2:1350672/140‑1 (MQ=11)

       tataCACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTa                                                                                                                                       <  1:1322961/138‑1 (MQ=255)

         taCACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAatat                                                                                                                                   >  1:1254148/1‑140 (MQ=255)

         taCACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCCATCGTAAAAGCAATCACTTCTCCATGGTATACATCTAatat                                                                                                                                   >  2:661848/1‑140 (MQ=255)

         taCACAGAACCTTGGTCACTATGTAACACCCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTCCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAatgt                                                                                                                                   >  2:1070517/1‑138 (MQ=255)

           cacaGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATAc                                                                                                                                 <  1:2354801/140‑1 (MQ=255)

           cacaGAAACTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGATTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTGTACATCTAATATAc                                                                                                                                 <  1:427223/140‑1 (MQ=255)

              aGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTCTCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTACTATACTgg                                                                                                                              >  2:1612061/1‑140 (MQ=11)

              aGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTgg                                                                                                                              <  1:519316/140‑1 (MQ=255)

              aGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAATGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTgg                                                                                                                              >  1:1652329/1‑140 (MQ=255)

              aGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTgg                                                                                                                              >  2:3428892/1‑140 (MQ=255)

               gAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAATTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGa                                                                                                                             >  1:3614747/1‑140 (MQ=11)

                   cTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAgg                                                                                                                         <  2:1464939/140‑1 (MQ=255)

                   cTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAgg                                                                                                                         <  2:1244985/140‑1 (MQ=255)

                          aCTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAAtt                                                                                                                  >  1:3826429/1‑140 (MQ=255)

                            tATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGt                                                                                                                >  1:3563338/1‑140 (MQ=255)

                            tATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGt                                                                                                                >  2:1665334/1‑140 (MQ=255)

                            tATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGt                                                                                                                >  1:19615/1‑140 (MQ=255)

                            tATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGt                                                                                                                >  2:3003330/1‑140 (MQ=255)

                              tGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGttt                                                                                                              >  1:3984577/1‑140 (MQ=255)

                               gTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTc                                                                                                             <  2:3045088/140‑1 (MQ=255)

                               gTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGAAGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTc                                                                                                             <  1:1888807/140‑1 (MQ=11)

                                tAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCt                                                                                                            <  1:1516170/140‑1 (MQ=255)

                                tAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCt                                                                                                            <  1:1449018/140‑1 (MQ=255)

                                tAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCt                                                                                                            <  1:3479539/140‑1 (MQ=255)

                                 aaCACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTg                                                                                                           >  2:115163/1‑140 (MQ=255)

                                  acacGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGt                                                                                                          >  1:925463/1‑140 (MQ=255)

                                     cGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCa                                                                                                       <  2:3152855/140‑1 (MQ=255)

                                      gctgTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCAATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCAt                                                                                                      <  2:969954/137‑1 (MQ=255)

                                      gCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTCCAATTGTTTCTGTCCAt                                                                                                      >  1:3733093/1‑140 (MQ=11)

                                      gCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACCATTGTTTCTGTCCAt                                                                                                      >  2:2434195/1‑140 (MQ=11)

                                      gCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCAt                                                                                                      >  2:3598012/1‑140 (MQ=255)

                                      gCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCAt                                                                                                      >  2:857855/1‑140 (MQ=255)

                                      gCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCAt                                                                                                      >  1:2786449/1‑140 (MQ=255)

                                      gCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCAt                                                                                                      >  2:424390/1‑140 (MQ=255)

                                      gCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCAt                                                                                                      >  2:1823158/1‑140 (MQ=255)

                                       cAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCACTCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATa                                                                                                     <  1:2682409/140‑1 (MQ=11)

                                       cAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATa                                                                                                     <  2:2696194/140‑1 (MQ=255)

                                        aGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGACAGGTACACTTGTTTCTGTCCATac                                                                                                    >  2:1561173/1‑139 (MQ=11)

                                        aGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATaa                                                                                                    >  1:1223227/1‑140 (MQ=255)

                                        aGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATaa                                                                                                    >  1:578324/1‑140 (MQ=255)

                                        aGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATaa                                                                                                    >  2:321633/1‑140 (MQ=255)

                                          ttCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAgg                                                                                                  <  2:3485516/140‑1 (MQ=255)

                                            ctcAGGCCGTGTTGGTAGCTGATGAAGTGTGTCTAAGACACAGTCTGTGTCCTGCTTATCCCCCCTCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCa                                                                                                <  1:1600385/140‑1 (MQ=11)

                                            ctcAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCa                                                                                                <  1:3218163/140‑1 (MQ=255)

                                            ctcAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCa                                                                                                <  2:2826382/140‑1 (MQ=255)

                                               aGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAat                                                                                             >  2:188480/1‑140 (MQ=255)

                                               aGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAat                                                                                             >  1:2048225/1‑140 (MQ=255)

                                                    gtgtTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATAtgtg                                                                                        <  1:3022956/140‑1 (MQ=255)

                                                    gtgtTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATAtgtg                                                                                        >  2:59878/1‑140 (MQ=255)

                                                       ttGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGaa                                                                                                                            >  2:4070173/1‑101 (MQ=11)

                                                       ttGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGaa                                                                                                                            <  1:4070593/101‑1 (MQ=11)

                                                               tGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTAc                                                                             >  1:3939712/1‑140 (MQ=255)

                                                               tGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTAc                                                                             >  2:536326/1‑140 (MQ=255)

                                                                gATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACt                                                                            <  2:3970152/140‑1 (MQ=12)

                                                                gATGAAGTGTGTATAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAATGTACATTTGTTTCTGTCAATTATTCAAATATGTGATGTCAGtttat                                                                            <  2:2054765/140‑4 (MQ=11)

                                                                 atgaaGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTa                                                                           >  1:424176/4‑140 (MQ=12)

                                                                        aTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTc                                                                    >  1:3380143/1‑140 (MQ=40)

                                                                        aTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTc                                                                    >  1:2602128/1‑140 (MQ=40)

                                                                         tGTCTAAGACAAAGACTGTGTCCTCCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTCTAAATCTAATATACTGGACAGGTACAAGTGTTTCTGTCCATAAGGCCAATATGGCATTTCGGTGACTAAtttttga                                                                   >  1:448869/1‑138 (MQ=11)

                                                                           tCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAag                                                                 >  1:1736750/1‑140 (MQ=40)

                                                                           tCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAag                                                                 >  2:1010423/1‑140 (MQ=40)

                                                                           tCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAag                                                                 >  1:2874083/1‑140 (MQ=40)

                                                                             tAAGAAAAAGTCTGTGTCCTGCTGATCCCCAATCTTAACAGCACTCACTTCTCCATTGTATAAATCTAATATACTGGCAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTTCTAGTTTTTCAagag                                                               <  2:2556560/140‑1 (MQ=11)

                                                                                     aGTCTGTGTCCTGCTTATCCCCAATCGTAAATGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTAGTAGTTTTTCAAGAGGGCGATCa                                                       >  1:2468218/1‑140 (MQ=255)

                                                                                     aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTCCTAGTGTGTCAAGAGGGCGATCa                                                       >  2:2918731/1‑140 (MQ=255)

                                                                                     aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCa                                                       >  2:3370254/1‑140 (MQ=40)

                                                                                     aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCa                                                       >  1:4169024/1‑140 (MQ=21)

                                                                                           tgtCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑a                                                <  1:2723411/140‑1 (MQ=21)

                                                                                                 gCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTc                                          >  2:1418138/1‑140 (MQ=40)

                                                                                                 gCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTc                                          <  2:3838045/140‑1 (MQ=40)

                                                                                                 gCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑AAAGTTc                                          <  2:4152144/140‑1 (MQ=21)

                                                                                                 gCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑AAAGTTc                                          <  1:632058/140‑1 (MQ=21)

                                                                                                 gCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑AAAGTTc                                          >  1:1153415/1‑140 (MQ=21)

                                                                                                   ttATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCg                                        <  2:3927453/140‑1 (MQ=21)

                                                                                                     aTCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGACAGGTACAATTGTTTCTGTCCATAAGGCCAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGAt                                      >  1:799319/1‑140 (MQ=255)

                                                                                                      tCCCCCATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATc                                     <  1:3327053/140‑1 (MQ=37)

                                                                                                      tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATc                                     <  1:2178315/140‑1 (MQ=17)

                                                                                                      tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTGTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATc                                     <  1:1855205/140‑1 (MQ=12)

                                                                                                        cccAATCGTAAATTCAATCACTTCTTCCTTGTATATATCTAATATTCTTGATATGTACACTTGTTTCTGTCCATAAGGCAATTATGTGATGTCCGTTACTAATTTTTCAAGAGGTCGATCAGACTG‑AATGTTCCGATCCa                                   <  2:2101255/140‑1 (MQ=11)

                                                                                                        cccAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCa                                   <  2:3689330/140‑1 (MQ=35)

                                                                                                        cccAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCa                                   <  1:1371704/140‑1 (MQ=11)

                                                                                                            aTCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAatat                               >  2:2364194/1‑140 (MQ=34)

                                                                                                                     gCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGAc                      <  2:4266154/140‑1 (MQ=32)

                                                                                                                     gCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGAc                      <  1:228122/140‑1 (MQ=32)

                                                                                                                       aaTCACTTCTCCATTGTATACCTCTAATATACTGGAAAGGTACAATTGTTTCTGTCCAGAAGGCAAATATGTGATGTCCGTTACGACTCTTTCAAGAGGGCGATCCGACTG‑AAAGGTCCGATCCATTATATTATCGACCa                    <  1:241206/140‑1 (MQ=11)

                                                                                                                       aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATCTATTATCGACCa                    >  1:1808007/1‑140 (MQ=17)

                                                                                                                       aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCa                    <  1:295107/140‑1 (MQ=32)

                                                                                                                       aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCa                    <  1:112104/140‑1 (MQ=32)

                                                                                                                       aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCa                    <  1:71596/140‑1 (MQ=32)

                                                                                                                            cTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCACGGCa               <  1:1010423/140‑1 (MQ=32)

                                                                                                                               ctcCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCACGGCatat            >  1:3866425/1‑140 (MQ=32)

                                                                                                                                  ccttGTATACATCTAATATCCTGGAAAGGGACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACGAGTTTTTCAAGAGGGCGATCAGACTGAAAAGTTCCGATCCAAGATATTATCGACCACGGCATATgg          <  2:2273283/138‑1 (MQ=11)

                                                                                                                                     tGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGc      >  2:2626611/1‑140 (MQ=32)

                                                                                                                                     tGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGc      >  2:789088/1‑140 (MQ=32)

                                                                                                                                       tataCATCTAATATACTGGAAAGGTCCAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGccc    >  1:3115311/1‑140 (MQ=17)

                                                                                                                                       tataCATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGccc    >  1:1031020/1‑140 (MQ=32)

                                                                                                                                         taCATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGCCCAt  >  2:1964688/1‑140 (MQ=32)

TAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGCCCAT  >  NZ_CP034943/3285508‑3285784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`