BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,328,921	G→A	5.1%	S258S (TCG→TCA)	lutP →	L‑lactate permease LutP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,328,921	0	G	A	5.1%	384.6 / 7.4	117	S258S (TCG→TCA)	lutP	L‑lactate permease LutP
Reads supporting (aligned to +/- strand): ref base G (74/37); new base A (3/3); total (77/40)
Fisher's exact test for biased strand distribution p-value = 4.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.89e-01

Only 100 of 123 total aligned reads displayed.

TCATCATGAGCGGGTTCAGGAAAGCCTTTGAGGTCTGGCCAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGAAACGGCGAAAACGAATCCAGCAGCGCCTGCGTACAGCGGCGGACAAATTT  >  NZ_CP034943/3328782‑3329060

tcatcatGAGCGGGTTCAGGAAAGCCTTTGAGGTCTGGCCAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCg                                                                                                                                             >  1:3628289/1‑140 (MQ=255)

tcatcatGAGCGGGTTCAGGAAAGCCTTTGAGGTCTGGCCAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCg                                                                                                                                             >  1:564178/1‑140 (MQ=255)

tcatcatGAGCGGGTTCAGGAAAGCCTTTGAGGTCTGGCCAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCg                                                                                                                                             >  2:3572096/1‑140 (MQ=255)

tcatcatGAGCGGGTTCAGGAAAGCCTTTGAGGTCTGGCCAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCg                                                                                                                                             >  2:1791478/1‑140 (MQ=255)

  atcatGAGCGGGTTCAGGAAAGCCTTTGAGGTCTGGCCAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGAt                                                                                                                                           >  1:89880/1‑140 (MQ=255)

  atcatGAGCGGGTTCAGGAAAGCCTTTGAGGTCTGGCCAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGAt                                                                                                                                           >  2:2189697/1‑140 (MQ=255)

  atcatGAGCGGGTTCAGGAAAGCCTTTGAGGTCTGGCCAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCAAt                                                                                                                                           >  1:2007588/1‑140 (MQ=255)

     atGAGCGGGTTCAGGAAAGCCTTTGAGGTCTGGCCAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGc                                                                                                                                        <  2:2644017/140‑1 (MQ=255)

       gAGCGGGTTCAGGAAAGCCTTTGAGGTCTGGCCAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTg                                                                                                                                      <  1:3572096/140‑1 (MQ=255)

           gggTTCAGGAAAGCCTTTGAGGTCTGGCCAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCt                                                                                                                                  <  2:1750353/140‑1 (MQ=255)

           gggTTCAGGAAAGCCTTTGAGGTCTGGCCAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCt                                                                                                                                  >  1:2258544/1‑140 (MQ=255)

             gTTCAGGAAAGCCTTGGAGATCTGGCCGGCGATTCTCGTATCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTTTTAGGGCCTGAGCTGCCCGATGTTTTGTCAGCTCTTGTTTCAATGGCTGCGCTTg                                                                                                                                >  2:2963225/1‑140 (MQ=255)

             gTTCAGGAAAGCCTGTGAGGTCTGGCCAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCGGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTg                                                                                                                                <  1:1195711/140‑1 (MQ=255)

                  ggAAAGCCTTTGAGGTCTGGCCAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGTtgtg                                                                                                                           >  2:3620572/1‑140 (MQ=255)

                  ggAAAGCCTTTGAGGTCTGGCCAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCtgtg                                                                                                                           >  2:3482028/1‑140 (MQ=255)

                   gAAAGCCTTTGAGGTCTGGCCAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTgtgt                                                                                                                          <  2:3628289/140‑1 (MQ=255)

                       gcgtttGAGGTCTGGCCAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGttttt                                                                                                                      >  1:2986536/4‑140 (MQ=255)

                       gCCTTTGAGGTCTGGCCAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGttttt                                                                                                                      >  1:411459/1‑140 (MQ=255)

                       gCCTTTGAGGTCTGGCCAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGttttt                                                                                                                      >  1:3920822/1‑140 (MQ=255)

                       gCCTTTGAGGTCTGGCCAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGttttt                                                                                                                      >  1:3128167/1‑140 (MQ=255)

                       gCCGTTGAGGTCTGGCCAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGttttt                                                                                                                      >  1:1202196/1‑140 (MQ=255)

                        ccTTTGAGGTCTGGCCAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTg                                                                                                                     >  2:4212284/1‑140 (MQ=255)

                        ccTTTGAGGTCTGGCCAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTCTTTg                                                                                                                     >  1:1256105/1‑140 (MQ=255)

                           ttGAGGTCTGGCCAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGaaa                                                                                                                  >  2:4216176/1‑140 (MQ=255)

                           ttGAGGTCTGGCCAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGaaa                                                                                                                  >  2:830006/1‑140 (MQ=255)

                           ttGAGGTCTGGCCAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGaaa                                                                                                                  >  1:2529431/1‑140 (MQ=255)

                           tggAGGTCTGGCCAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGaaa                                                                                                                  >  2:4254066/3‑140 (MQ=255)

                                  cTGGCCAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGa                                                                                                           >  2:3835696/1‑140 (MQ=255)

                                  cTGGCCAGCGATTCTCGTTTCCGGTGTTGCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGa                                                                                                           >  1:3212661/1‑140 (MQ=255)

                                   tGGCCAGCGCTTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCGTGTGCTGCCGGATGTGTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGGTGTGTTTTTGAACTGGTGGac                                                                                                          >  2:3610687/1‑139 (MQ=255)

                                   tGGCCAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGac                                                                                                          >  2:597030/1‑139 (MQ=255)

                                   tGGCCAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGaa                                                                                                          >  2:4266849/1‑140 (MQ=255)

                                   tGGCCAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGaa                                                                                                          >  2:2467810/1‑140 (MQ=255)

                                   tGGCCAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGaa                                                                                                          >  2:475565/1‑140 (MQ=255)

                                   tGGCCAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGaa                                                                                                          >  1:1885215/1‑140 (MQ=255)

                                   tGGCCAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGaa                                                                                                          >  2:936393/1‑140 (MQ=255)

                                   tGGCCAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGaa                                                                                                          >  2:1057113/1‑140 (MQ=255)

                                     gCCAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGc                                                                                                        >  1:275295/1‑140 (MQ=255)

                                     gCCAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGc                                                                                                        >  1:3708779/1‑140 (MQ=255)

                                      ccAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGcc                                                                                                       <  2:89880/140‑1 (MQ=255)

                                      ccAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCAATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGcc                                                                                                       <  2:2007588/140‑1 (MQ=255)

                                         gCGATTCTCGGTTCCGGTGTTTCCTGTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGaa                                                                                                    <  2:1881554/140‑1 (MQ=255)

                                          cGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCCGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCGTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGaaa                                                                                                   >  2:1660670/1‑140 (MQ=255)

                                          cGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGaaa                                                                                                   >  2:1661125/1‑140 (MQ=255)

                                           gATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGaaaa                                                                                                  <  1:3268129/140‑1 (MQ=255)

                                                         gtgtTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGttt                                                                                    >  1:895668/1‑140 (MQ=255)

                                                         gtgtTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGttt                                                                                    >  1:4165280/1‑140 (MQ=255)

                                                             ttCCTGTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGTATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCa                                                                                <  1:2194072/140‑1 (MQ=255)

                                                              tCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCaa                                                                               <  1:2158260/140‑1 (MQ=255)

                                                                  tcgCTGTTGTTCAATCTCTCAGTTCCAATTTCTGAGGGCCGGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTGTTTGAAATGGGGGAAGGCGAAAACAACATTCCGGTTTGCCAATGaa                                                                           <  2:2709579/138‑1 (MQ=255)

                                                                    gCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACa                                                                         >  2:3371829/1‑140 (MQ=255)

                                                                          gttCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGaagc                                                                   >  2:3504779/1‑138 (MQ=255)

                                                                          gttCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATc                                                                   >  2:581045/1‑140 (MQ=255)

                                                                                tatCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTgccgc                                                             >  2:3842082/1‑140 (MQ=255)

                                                                                tatCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTgccgc                                                             >  2:3684978/1‑140 (MQ=255)

                                                                                 atCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTgccgcc                                                            <  2:2529431/140‑1 (MQ=255)

                                                                                     cAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGa                                                        >  1:3377944/1‑140 (MQ=255)

                                                                                        ttCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTg                                                     >  1:2323679/1‑140 (MQ=255)

                                                                                        ttCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTg                                                     >  1:2520552/1‑140 (MQ=255)

                                                                                          ccAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAAGGAACAGGAATCTGCCGCCGCGATTGaa                                                   >  1:2046266/1‑140 (MQ=255)

                                                                                           gaaTTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGaaa                                                  <  1:3812285/139‑1 (MQ=255)

                                                                                           ccatgtCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGaaa                                                  <  2:1329611/135‑1 (MQ=255)

                                                                                           cAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGaaa                                                  <  1:1868451/140‑1 (MQ=255)

                                                                                           cAAGTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGaaa                                                  <  1:3431664/140‑1 (MQ=255)

                                                                                             aTTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGAAACg                                                >  2:3212387/1‑140 (MQ=255)

                                                                                             aTTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGAAACg                                                >  2:1166846/1‑140 (MQ=255)

                                                                                             aTTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGAAACg                                                >  2:270363/1‑140 (MQ=255)

                                                                                             aTTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTAAGCGCTTGTTTCGATGGATGCGCTTGATGTGTTTTTGAAAAGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGAAAcc                                                >  2:2740163/1‑139 (MQ=255)

                                                                                             aTTTCTTAGGGCCTGAGCTGCCGCATGGTTTCTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTCTTTTTGAAATGGTGGAAGCCGAAAACAACATTCAGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGAAACg                                                >  2:74424/1‑140 (MQ=255)

                                                                                                tCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGAAACGGCg                                             <  2:395645/140‑1 (MQ=255)

                                                                                                  ttAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGAAACGGCGaa                                           >  1:4227056/1‑140 (MQ=255)

                                                                                                  ttAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGAAACGGCGaa                                           >  1:65635/1‑140 (MQ=255)

                                                                                                     gggCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGAAACGGCGAAAAc                                        >  1:117953/1‑140 (MQ=255)

                                                                                                     gggCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGAAACGGCGAAAAc                                        >  1:840148/1‑140 (MQ=255)

                                                                                                         cTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGAAACGGCGAAAACGAAt                                    <  1:4266849/140‑1 (MQ=255)

                                                                                                            aGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGAAACGGCGAAAACGAATCca                                 >  2:3306823/1‑140 (MQ=255)

                                                                                                            aGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGAAACGGCGAAAACGAATCca                                 >  1:2331611/1‑140 (MQ=255)

                                                                                                            aGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGAAACGGCGAAAACGAATCca                                 >  1:2442435/1‑140 (MQ=255)

                                                                                                            aGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGAAACGGCGAAAACGAATCca                                 >  1:4063947/1‑140 (MQ=255)

                                                                                                              cTGCCGGATGTTTGGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTGGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGAAACGGCGAAAACGAATCcagc                               <  1:2221670/140‑1 (MQ=255)

                                                                                                                gCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGAAACGGCGAAAACGAATCcagcag                             <  1:1166846/140‑1 (MQ=255)

                                                                                                                 ccGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGAAACGGCGAAAACGAATCCagcagc                            >  2:1971640/1‑140 (MQ=255)

                                                                                                                 ccGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGAAACGGCGAAAACGAATCCagcagc                            >  2:310941/1‑140 (MQ=255)

                                                                                                                 ccGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGAAACGGCGAAAACGAATCCagcagc                            >  2:3127989/1‑140 (MQ=255)

                                                                                                                  cGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGAAACGGCGAAAACGAATCCAGCAgcg                           <  1:3306823/140‑1 (MQ=255)

                                                                                                                   cgATGTTTTGTCAGCTCTTGTTTCAATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCGGTGAACAAGAGTCGGCCGCCTCAATTGAAACGGCGCGCACAAATCCGGCGgcgc                          <  1:940529/139‑1 (MQ=255)

                                                                                                                     attttttGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGAAACGGCGAAAACGAATCCAGCAGCGCCt                        <  2:888927/137‑1 (MQ=255)

                                                                                                                     aTGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGGTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGAAACGGCGAATACGAATCCAGCAGCGCCt                        <  1:799649/140‑1 (MQ=255)

                                                                                                                     aTGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGAAACGGCGAAAACGAATCCAGCAGCGCCt                        <  1:1818546/140‑1 (MQ=255)

                                                                                                                     aTGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGAAACGGCGAAAACGAATCCAGCAGCGCCt                        <  1:4139932/140‑1 (MQ=255)

                                                                                                                     aTGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGAAACGGCGAAAACGAATCCAGCAGCGCCt                        <  2:2995693/140‑1 (MQ=255)

                                                                                                                     aTGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGAAACGGCGAAAACGAATCCAGCAGCGCCt                        <  1:2189697/140‑1 (MQ=255)

                                                                                                                     aTGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGAAACGGCGAAAACGAATCCAGCAGCGCCt                        <  2:1617845/140‑1 (MQ=255)

                                                                                                                     aTGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTCTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGAAACGGCGAAAACGAATCCAGCAGCGCCt                        <  2:1256105/140‑1 (MQ=255)

                                                                                                                     aTGTTTGGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGAAACGGCGAAAACGAATCCAGCAGCGCCt                        <  2:570223/140‑1 (MQ=255)

                                                                                                                          ttGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGAAACGGCGAAAACGAATCCAGCAGCGCCTGCGTa                   <  1:290690/140‑1 (MQ=255)

                                                                                                                           tGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGAAACGGCGAAAACGAATCCAGCAGCGCCTGCGTAc                  >  1:611242/1‑140 (MQ=255)

                                                                                                                                      gTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGAAACGGCGAAAACGAATCCAGCAGCGCCTGCGTACAGCGGCGGACa       >  2:1541653/1‑140 (MQ=255)

                                                                                                                                      gTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGAAACGGCGAAAACGAATCCAGCAGCGCCTGCGTACAGCGGCGGACa       >  2:2135726/1‑140 (MQ=255)

                                                                                                                                           gATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGAAACTGCGAAAACGAATCCAGCAGCGCCTGCGTACAGCGGCGGACAAAttt  >  1:3903249/1‑140 (MQ=255)

TCATCATGAGCGGGTTCAGGAAAGCCTTTGAGGTCTGGCCAGCGATTCTCGTTTCCGGTGTTTCCTTTGCTGTTGTTCAATATCTCAGTTCCAATTTCTTAGGGCCTGAGCTGCCGGATGTTTTGTCAGCGCTTGTTTCGATGGCTGCGCTTGCTGTGTTTTTGAAATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCAATGAACAGGAATCTGCCGCCGCGATTGAAACGGCGAAAACGAATCCAGCAGCGCCTGCGTACAGCGGCGGACAAATTT  >  NZ_CP034943/3328782‑3329060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`