Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 452,125 T→A 46.3% K146I (AAA→ATA)  EO946_RS02320 ← dienelactone hydrolase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943452,1250TA46.3% 90.6 / inf 261K146I (AAA→ATA) EO946_RS02320dienelactone hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base T (61/78);  new base A (58/63);  total (119/142)
Fisher's exact test for biased strand distribution p-value = 5.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 267 total aligned reads displayed.

TTCTGCCTCGTTTTTGATGATGGAAAGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGCATA  >  NZ_CP034943/451989‑452262
                                                                                                                                        |                                                                                                                                         
ttCTGCCTCGTTTTTGATGATGGAAAGCGTTTTGAAGACGCGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGttttt                                                                                                                                        <  1:1044405/140‑1 (MQ=255)
  cTGCCTCGTTTTTGATGATGGAAAGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCCTGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTg                                                                                                                                      >  1:2131402/1‑140 (MQ=255)
  cTGCCTCGTTTTTGATGATGGAAAGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATTCTGTTGTTCCCTCAGTTTTTTg                                                                                                                                      >  1:3331267/1‑140 (MQ=255)
    gCCTCGTTTTTGATGATGGAAAGCGTTTTGAATAATAGTGGCCTGACAAAATAAACCGACTCGTGATTGGCAAAGCCATGAAGAGCATAAAATTGATAAATCTCCAAATTGGGATTCTGTTGTTCCCTCAGTATTTTGAt                                                                                                                                    <  1:248841/140‑1 (MQ=255)
            tttGATGATGGAAAGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCg                                                                                                                            <  1:3294513/140‑1 (MQ=255)
               gattttgGAAAGCGTTTTGAAGAAGAGTGGCATGTCAAAATAAACCGACTCGGGATTGGAAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCaa                                                                                                                         <  1:1004257/135‑1 (MQ=255)
                   atgGAAAGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTTGCAAAGCCATTAATAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCACTCAGTTATTTGATAAGAACCGCGACATc                                                                                                                     >  1:2740416/1‑140 (MQ=255)
                    tgGAAAGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCa                                                                                                                    >  1:3716177/1‑140 (MQ=255)
                       aatgggTTTTTAACAAGTTTGCCCTTTAAAAAAAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTTTTGTTCCCTCAGTATTTTTATAAGAACCGCAACATCAAac                                                                                                                 <  1:3267107/135‑1 (MQ=255)
                         tgCGTTTTTAAGTTGAGTGGCGTGTAAAAATAAACGGACTCTGTATTGGAAAAGCAATGAAGAGAATCAAATTGATAAATCTCAAAATTGGGATGCTGTTTTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAacga                                                                                                               <  1:3883808/139‑1 (MQ=255)
                         aggtttttGAAGAACAGGGGCCTGAAAAAAAAAACCGACCGGGGATTGGCAAAGCCTTGAAGAGCATCAAATTGATAATTCTCCAATTTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAacga                                                                                                               <  1:3682817/136‑1 (MQ=255)
                         aGCGTTTTTAAGAAGCTTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAacga                                                                                                               <  2:4299148/140‑1 (MQ=255)
                         aGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAacga                                                                                                               <  2:2744397/140‑1 (MQ=255)
                              tttGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGtt                                                                                                          >  2:544864/1‑140 (MQ=255)
                                          tGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGa                                                                                              >  2:3839119/1‑140 (MQ=255)
                                            gcatGTCAAAATAAACCGACTCGGTATTGGCATAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGTTGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                            <  2:2041252/137‑1 (MQ=255)
                                            gCCTTTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGAATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAATAACCGCAACATCACACGACGGTTCATATGAAGGGaaa                                                                                            <  1:4073677/140‑1 (MQ=255)
                                            gCCTTTAAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTTATAAATCTCCAAATTGGGATGCTTTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                            <  2:3103505/140‑1 (MQ=255)
                                            gCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                            >  1:4207838/1‑140 (MQ=255)
                                            gCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                            <  2:2501932/140‑1 (MQ=255)
                                            gCCTGTAAAAATAAACCGACTCGGTATTGGCAAAGCCATGAATAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGAAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                            <  1:1159130/140‑1 (MQ=255)
                                            gCCTGTAAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACAACAAACGACGGTTCATATGAAGGGaaa                                                                                            <  1:2105299/140‑1 (MQ=255)
                                            gCCTGTAAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGAATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                            <  1:1657740/140‑1 (MQ=255)
                                            gCCTGACAAAATACACCGACTCGGGATTGGCAAAGCCATGACGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCATTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                            <  1:3552084/140‑1 (MQ=255)
                                            gCCTGACAAAATAAACCGACTCGGGTTTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                            <  1:1711381/140‑1 (MQ=255)
                                               tGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaaaaa                                                                                         >  1:1912181/1‑139 (MQ=255)
                                               tGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAAc                                                                                         >  1:716367/1‑140 (MQ=255)
                                                 tCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACaa                                                                                       >  1:1866220/1‑140 (MQ=255)
                                                 tCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACaa                                                                                       >  1:1596947/1‑140 (MQ=255)
                                                 tCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACaa                                                                                       >  1:3452405/1‑140 (MQ=255)
                                                 tCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACaa                                                                                       >  1:38153/1‑140 (MQ=255)
                                                      aTAAACCGACTCGGTATTGGCAAAGCCATGAAGAGCATCAAATTGTTAATTCTCAAAATTGGTATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGa                                                                                  <  2:4233833/140‑1 (MQ=255)
                                                      aTAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGa                                                                                  <  1:544864/140‑1 (MQ=255)
                                                       tAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGACGGGAAAAACACTAGGAc                                                                                 >  2:348269/1‑140 (MQ=255)
                                                       tAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGAc                                                                                 >  2:2834417/1‑140 (MQ=255)
                                                          aCCGACTCGGTATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCgg                                                                              <  2:1647045/140‑1 (MQ=255)
                                                          aCCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCgg                                                                              >  1:1929370/1‑140 (MQ=255)
                                                           ccGACTCGGGATTGGAAAAGCCATGAAGAGCATCAAATTGATAACTCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCCACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCggg                                                                             <  2:2850953/140‑1 (MQ=255)
                                                              aCTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAg                                                                          >  1:828225/1‑140 (MQ=255)
                                                              aCTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAg                                                                          >  2:909069/1‑140 (MQ=255)
                                                               cTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGa                                                                         >  2:2268296/1‑140 (MQ=255)
                                                               cTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGa                                                                         >  2:4135513/1‑140 (MQ=255)
                                                                    gATTGGCAAAGCCATGAAGACCTTCAAATTTATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGTTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGc                                                                    <  2:4205721/140‑1 (MQ=255)
                                                                      ttGGAAAAGCCATGAAGAGCATAAAATTTATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCAc                                                                  <  1:2704616/140‑1 (MQ=255)
                                                                        ggCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGt                                                                >  1:4316562/1‑140 (MQ=255)
                                                                              tccATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTATTGATAAGAACCGCAACATCAAACGAAGGTTCATATGAAGGGAAAAACAATAGGACCGGGAAGACTGGCACCTGATGaa                                                          >  2:4177652/2‑140 (MQ=255)
                                                                              gCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGaa                                                          >  2:2633257/1‑140 (MQ=255)
                                                                              gCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGaa                                                          >  2:2110464/1‑140 (MQ=255)
                                                                              gCCAGGAAGAGCATCAAATTGATAAATCTCCAAATTGAGATTCTGTTGTTGCCTCAGTATTTTGATACTAACCGCAACATCAAACTACGGTGCAGATATAGGGAAAAACAATAGGACCGGGCCGGCTGGCACGTGATGaa                                                          >  2:1693218/1‑140 (MQ=255)
                                                                                  tGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAgaga                                                      >  2:392244/1‑140 (MQ=255)
                                                                                   gAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAATAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGaa                                                     >  1:2028965/1‑140 (MQ=255)
                                                                                   gAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCggg                                                                             <  1:2661451/116‑1 (MQ=255)
                                                                                   gAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGaa                                                     >  1:3065460/1‑140 (MQ=255)
                                                                                   gAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGaa                                                     >  1:1968221/1‑140 (MQ=255)
                                                                                       agCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATcgc                                                 >  1:500686/1‑140 (MQ=255)
                                                                                       agCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATcgc                                                 >  1:1382988/1‑140 (MQ=255)
                                                                                       agCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATcgc                                                 >  1:4133169/1‑140 (MQ=255)
                                                                                        tcATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATcgcg                                                <  2:1320176/139‑1 (MQ=255)
                                                                                        caaTCAAATTGATAAATCTCAATATTTGGTTGCTGTTGTTCCATATTTATTTTTATAATAACCTCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATcgcg                                                <  1:379707/138‑1 (MQ=255)
                                                                                         cATCAAATTGATAAATCTCCACATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCCAACGACGGTGCATATGAAGGGAAAAAAAATAGGACCGGTCCGACTGGCACGTGATGACGAGCATcgcga                                               >  2:3707029/1‑139 (MQ=255)
                                                                                         cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGt                                               >  1:4179272/1‑140 (MQ=255)
                                                                                         cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGt                                               >  2:3021889/1‑140 (MQ=255)
                                                                                         cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGt                                               >  1:3020056/1‑140 (MQ=255)
                                                                                         cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACAGGGCAAACTGGCACGTGATGAAGAGAATcgctt                                               >  2:3678555/1‑138 (MQ=255)
                                                                                         cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAAAAATAGGACCGGGCAGACTGGCACGTGATGAAGAGACTCGCGt                                               >  1:2337644/1‑140 (MQ=255)
                                                                                         cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGGTCATATGAAGCGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGt                                               >  2:2757169/1‑140 (MQ=255)
                                                                                                ttGATAAATCTCCAAATTGGGATGCTGTTGGTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCggg                                        <  2:637675/140‑1 (MQ=255)
                                                                                                ttGATAAATCTCCAAAGTGGGATTCTGTTGTTCCCTCAGTTTTTTGATAAGACCCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCggg                                        <  2:2594854/140‑1 (MQ=255)
                                                                                                 tGATAAATCGCCAAATTGGCATGCTGTTGTTCCCTCAGTACTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGa                                       <  1:782652/140‑1 (MQ=255)
                                                                                                   aTAAATCTCCAACTGGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAAc                                     <  2:3358393/140‑1 (MQ=255)
                                                                                                    tAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAAcc                                    >  1:481679/1‑140 (MQ=255)
                                                                                                    tAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAAcc                                    >  1:2609602/1‑140 (MQ=255)
                                                                                                     aaaTCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTTATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCg                                   <  1:525925/140‑1 (MQ=255)
                                                                                                        tctcCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGACCCGtaa                                >  2:2342304/1‑140 (MQ=255)
                                                                                                                  gggATGCTGTTGTGCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGACTGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTAACCGGGACCCGTAATAACAAACAg                      >  2:720333/1‑140 (MQ=255)
                                                                                                                    gctGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTCATAACAAACAGCt                    <  1:1781759/138‑1 (MQ=255)
                                                                                                                    gATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCt                    <  1:3141580/140‑1 (MQ=255)
                                                                                                                         ttttgttCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt               <  1:3466198/138‑1 (MQ=255)
                                                                                                                         tgttgtTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt               <  2:3399701/140‑1 (MQ=255)
                                                                                                                         tgttgtTCCCTCAGTTTTTGGATAAGACCCGCAACAACAAACGACGGTTCATATGAAGGGAAAAACAATCGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt               <  2:2924143/140‑1 (MQ=255)
                                                                                                                         tgttgtTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt               <  1:3380705/140‑1 (MQ=255)
                                                                                                                         tgttgtTCCATCAGATTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt               <  2:4071659/140‑1 (MQ=255)
                                                                                                                          gttgttCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTc              <  2:3325030/140‑1 (MQ=255)
                                                                                                                          gttgttCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTc              <  2:3802528/140‑1 (MQ=255)
                                                                                                                           ttgttCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCa             >  1:882662/1‑140 (MQ=255)
                                                                                                                               tCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTc         <  2:65428/140‑1 (MQ=255)
                                                                                                                                cccTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCa        >  2:1230443/1‑140 (MQ=255)
                                                                                                                                   tCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGACCCGTAATAACAAACAGCTCCGCTCACCTCAGGc     >  2:2609863/1‑140 (MQ=255)
                                                                                                                                   tCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGc     >  2:3450474/1‑140 (MQ=255)
                                                                                                                                   tCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGc     >  1:1462378/1‑140 (MQ=255)
                                                                                                                                   tCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGAGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGc     >  2:1916410/1‑140 (MQ=255)
                                                                                                                                   tCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGGTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAgga     >  1:677634/1‑139 (MQ=255)
                                                                                                                                   tCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGc     >  1:3723311/1‑140 (MQ=255)
                                                                                                                                    catttttttGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGCa    <  2:134866/137‑1 (MQ=255)
                                                                                                                                    cAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGCa    <  1:1306330/140‑1 (MQ=255)
                                                                                                                                    cAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGCa    <  2:3723311/140‑1 (MQ=255)
                                                                                                                                     aGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGCa  >  2:2216393/1‑139 (MQ=255)
                                                                                                                                     aGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGCAt   >  2:1089048/1‑140 (MQ=255)
                                                                                                                                     aGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGGATCCGGGAACCGAAATAACAAACAGCTCCGCTCACCTCAGGCAt   >  2:3875866/1‑140 (MQ=255)
                                                                                                                                     aGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGGCGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGACCCGTACTAACAAACAGCGCCGCTCACCGCAGGCAt   >  2:3759970/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                         
TTCTGCCTCGTTTTTGATGATGGAAAGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGCATA  >  NZ_CP034943/451989‑452262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: