Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,637,344 T→A 7.6% Y44N (TAC→AAC)  EO946_RS18740 → DUF2529 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,637,3440TA 7.6% 903.1 / 46.0 280Y44N (TAC→AAC) EO946_RS18740DUF2529 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (140/117);  new base A (15/5);  total (156/124)
Fisher's exact test for biased strand distribution p-value = 1.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

Only 100 of 348 total aligned reads displayed.

GGATTCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGATGTTTTGCTCAGGGACCGG  >  NZ_CP034943/3637205‑3637483
                                                                                                                                           |                                                                                                                                           
ggaTTCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATttatttat                                                                                                                                             >  1:4195795/1‑140 (MQ=255)
 gaTTCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTtata                                                                                                                                            >  2:519162/1‑140 (MQ=255)
 gaTTCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTtata                                                                                                                                            <  2:4947/140‑1 (MQ=255)
 gaTTCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTtata                                                                                                                                            >  1:1366317/1‑140 (MQ=255)
 gaTTCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTtata                                                                                                                                            >  1:3771420/1‑140 (MQ=255)
 gaTTCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTtata                                                                                                                                            <  2:2062086/140‑1 (MQ=255)
 gaTTCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTtata                                                                                                                                            <  2:3924820/140‑1 (MQ=255)
 gaTTCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTtata                                                                                                                                            <  2:1156503/140‑1 (MQ=255)
 gaTTCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTtata                                                                                                                                            <  1:519363/140‑1 (MQ=255)
 gaTTCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTtata                                                                                                                                            >  2:150585/1‑140 (MQ=255)
 gaTTCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTtata                                                                                                                                            <  1:2341089/140‑1 (MQ=255)
 gaTTCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTtata                                                                                                                                            >  2:2041701/1‑140 (MQ=255)
 gaTTCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGCGCATTCCATTTATTtata                                                                                                                                            >  2:2674329/1‑140 (MQ=255)
 gaTTCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGATTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTtata                                                                                                                                            <  2:2616695/140‑1 (MQ=255)
   ttCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACg                                                                                                                                          >  2:304857/1‑140 (MQ=255)
           tGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTCAt                                                                                                                                  >  1:4319739/1‑140 (MQ=255)
           tGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAAt                                                                                                                                  <  2:1392228/140‑1 (MQ=255)
           tGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAAt                                                                                                                                  <  2:3981192/140‑1 (MQ=255)
           tGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAAt                                                                                                                                  <  1:2735878/140‑1 (MQ=255)
            gTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTAGTTACACGGAGCTAATg                                                                                                                                 >  2:2298544/1‑140 (MQ=255)
                aaaaTATTTACGACGCAGTTAACAGGTATTTTTTCCCGGATTAAGTATAAGGAATCTGACGCGCTTTAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTATTGAgat                                                                                                                             >  2:1088695/1‑138 (MQ=255)
                aaaaTATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTtata                                                                                                                                            >  1:3159491/1‑125 (MQ=255)
                aaaaTATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTtata                                                                                                                                            <  2:3159491/125‑1 (MQ=255)
                aaaaTATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCt                                                                                                                             >  2:2728547/1‑140 (MQ=255)
                aaaaTATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCt                                                                                                                             >  2:2995872/1‑140 (MQ=255)
                aaaaTATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATAAGGAGCTAATGAGCt                                                                                                                             >  1:2210590/1‑140 (MQ=255)
                aaaaTATTTACGACGCAGTTAACAGGAATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCt                                                                                                                             >  1:835231/1‑140 (MQ=255)
                     atTTACGACGCAGTTACCAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAgg                                                                                                                        <  1:2230538/140‑1 (MQ=255)
                     atTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCATGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAgg                                                                                                                        <  2:2842882/140‑1 (MQ=255)
                      tttACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAggg                                                                                                                       <  2:319066/140‑1 (MQ=255)
                      tttACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAggg                                                                                                                       <  2:3882734/140‑1 (MQ=255)
                                    aaCAGGTATTTTTTCCCGCCTTCAGGATACGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAgg                                                                                                         <  2:2510351/140‑1 (MQ=255)
                                    aaCAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAgg                                                                                                         <  2:3608952/140‑1 (MQ=255)
                                         gTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCAcc                                                                                                    >  1:4252528/1‑140 (MQ=255)
                                         gTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCAcc                                                                                                    >  1:3249561/1‑140 (MQ=255)
                                          tATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCg                                                                                                   >  1:1921430/1‑140 (MQ=255)
                                            ttttttCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCCGGGCGTCTGTTATGAGGCCACCGaa                                                                                                 >  1:2806000/1‑140 (MQ=255)
                                            ttttttCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGaa                                                                                                 >  1:3870881/1‑140 (MQ=255)
                                            ttttttCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGaa                                                                                                 >  2:2098747/1‑140 (MQ=255)
                                            ttttttCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGaa                                                                                                 >  1:4108983/1‑140 (MQ=255)
                                            ttttttCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGaa                                                                                                 >  2:3221440/1‑140 (MQ=255)
                                            ttttttCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGaa                                                                                                 >  2:3646734/1‑140 (MQ=255)
                                             tttttCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGaaa                                                                                                <  1:2702454/140‑1 (MQ=255)
                                                ttCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAagca                                                                                             <  2:4194297/140‑1 (MQ=255)
                                                ttCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAagca                                                                                             <  2:956328/140‑1 (MQ=255)
                                                 tCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAagcag                                                                                            >  2:636919/1‑140 (MQ=255)
                                                 tCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAagcag                                                                                            <  1:2614828/140‑1 (MQ=255)
                                                 tCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAagcag                                                                                            >  1:1217790/1‑140 (MQ=255)
                                                   ccGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCg                                                                                          <  2:1580343/140‑1 (MQ=255)
                                                     gCATTCAGGATAAGGAATCTGACGCGATTGCAGATGGGGCGCGGCTGCGTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGaa                                                                                        <  2:739159/140‑1 (MQ=255)
                                                     gCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGaa                                                                                        <  2:2915636/140‑1 (MQ=255)
                                                      cATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAAc                                                                                       <  1:772146/140‑1 (MQ=255)
                                                               tAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCAt                                                                              <  1:1053431/140‑1 (MQ=255)
                                                               tAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCAt                                                                              <  1:469605/140‑1 (MQ=255)
                                                                aaGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATc                                                                             <  1:2000033/140‑1 (MQ=255)
                                                                       cTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGaa                                                                                                 <  2:876574/113‑1 (MQ=255)
                                                                       cTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAagca                                                                                             >  2:1851457/1‑117 (MQ=255)
                                                                       cTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCaaa                                                                      >  1:855508/1‑140 (MQ=255)
                                                                       cTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCaaa                                                                      >  2:1233607/1‑140 (MQ=255)
                                                                       cTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCaaa                                                                      >  2:3800552/1‑140 (MQ=255)
                                                                       cTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCaaa                                                                      >  2:3702127/1‑140 (MQ=255)
                                                                       cTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCaaa                                                                      >  1:589785/1‑140 (MQ=255)
                                                                       cTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCaaa                                                                      >  1:3699778/1‑140 (MQ=255)
                                                                       cTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCaaa                                                                      >  1:4281558/1‑140 (MQ=255)
                                                                          aCGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGcc                                                                   >  1:2949623/1‑140 (MQ=255)
                                                                              gATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTc                                                               >  2:4281241/1‑140 (MQ=255)
                                                                               aTTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCCCCGAAAGCAGCGAACCCTTCCCATCTCTCAAAGCCTTTcc                                                              >  2:3498508/1‑140 (MQ=255)
                                                                               aTTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTcc                                                              >  2:855398/1‑140 (MQ=255)
                                                                                      aTGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAgc                                                       <  1:3042389/140‑1 (MQ=255)
                                                                                        ggggCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAgcgc                                                     >  1:1395429/1‑140 (MQ=255)
                                                                                        ggggCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAgcgc                                                     >  2:4069674/1‑140 (MQ=255)
                                                                                        ggggCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAgcgc                                                     >  1:2627520/1‑140 (MQ=255)
                                                                                        ggggCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAgcgc                                                     >  1:241746/1‑140 (MQ=255)
                                                                                          ggCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTtata                                                                                                                                            >  2:4116992/1‑51 (MQ=255)
                                                                                          ggCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTg                                                   >  2:1551794/1‑140 (MQ=255)
                                                                                          ggCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCATTCCCATCTGTCAAAGACTTTCCAGAAAGCGCTg                                                   >  2:1726848/1‑140 (MQ=255)
                                                                                            cgcgGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAg                                                 <  1:159805/140‑1 (MQ=255)
                                                                                                gctgctTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAg                                             >  1:4057259/1‑140 (MQ=255)
                                                                                                gctgctTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTACCAGAAAGCGCTGAGGAAg                                             >  1:4179039/1‑140 (MQ=255)
                                                                                                    cttgctCACGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGAc                                         >  1:3196947/1‑140 (MQ=255)
                                                                                                    cttgctCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGAc                                         >  1:1818987/1‑140 (MQ=255)
                                                                                                     ttgctCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACa                                        <  2:2237549/140‑1 (MQ=255)
                                                                                                     ttgctCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACa                                        <  2:2134167/140‑1 (MQ=255)
                                                                                                       gctCAAGCGGTGATCAGCGGGCATTCAATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGa                                      <  2:3382211/140‑1 (MQ=255)
                                                                                                        ctCACGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCCAACCCTTCCCATCTTTCAAAGCCTTTCAAGAAAGCGCTGAGGAAGTGACATaa                                     <  2:1700161/140‑1 (MQ=255)
                                                                                                         tCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGaaa                                    >  1:334674/1‑140 (MQ=255)
                                                                                                         tCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGaaa                                    >  1:3942558/1‑140 (MQ=255)
                                                                                                           aaGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGc                                  >  1:3483328/1‑140 (MQ=255)
                                                                                                               ggTGATCCGCGGGCATTCAATTTATTTATACGGCGCTAATGCGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTTTCAAAGCCTTCCCAGAACGCGCTGAGGAAGTGACAGAACGCGACa                              <  1:2041701/140‑1 (MQ=255)
                                                                                                                  gATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACACAAATCGACAggg                           >  2:3264949/1‑140 (MQ=255)
                                                                                                                   aTCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGt                          >  1:1523302/1‑140 (MQ=255)
                                                                                                                   aTCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGt                          >  1:628555/1‑140 (MQ=255)
                                                                                                                            cATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGATGtt                 <  2:1350234/140‑1 (MQ=255)
                                                                                                                            cATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTACCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGATGtt                 <  2:1874074/140‑1 (MQ=255)
                                                                                                                                ccATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGATGTTTTGc             >  1:3563402/1‑140 (MQ=255)
                                                                                                                                        ttatACGGTGCTACTGAGGTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGATGTTTTGCTCAGGGAc     <  2:2348476/140‑1 (MQ=255)
                                                                                                                                          ataCGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGATGTTTTGCTCAGGGACCg   >  1:1137179/1‑140 (MQ=255)
                                                                                                                                           taCGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGATGTTTTGCTCAGGGACCgg  >  2:1485229/1‑140 (MQ=255)
                                                                                                                                           taCGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGATGTTTTGCTCAGGGACCgg  >  2:563158/1‑140 (MQ=255)
                                                                                                                                           taCGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCGGATGTTTTGCTCAGGGACCgg  >  2:4043267/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
GGATTCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGATGTTTTGCTCAGGGACCGG  >  NZ_CP034943/3637205‑3637483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: