Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,790,764 T→G 19.9% N203H (AAC→CAC)  EO946_RS19525 ← BglG family transcription antiterminator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,790,7640TG19.9% 438.7 / 43.6 209N203H (AAC→CAC) EO946_RS19525BglG family transcription antiterminator
Reads supporting (aligned to +/- strand):  ref base T (23/138);  new base G (46/2);  total (69/140)
Fisher's exact test for biased strand distribution p-value = 2.20e-26
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 210 total aligned reads displayed.

TTTTTGATGCAGGATATGATCCATATCTTCCGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCT  >  NZ_CP034943/3790630‑3790893
                                                                                                                                      |                                                                                                                                   
tttttGATGCAGGATATGATCCATATCTTCCGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCa                                                                                                                                <  2:1974253/140‑1 (MQ=255)
  tttGATGCAGGATATGATCCATATCTTCCGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTGAtt                                                                                                                              >  1:427117/1‑140 (MQ=255)
       tGCAGGATATTATCCTTATATTCCGGGAAAAGATATACATAGTTTTGGGTTCTGATCAGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCAttttttt                                                                                                                         <  2:3985777/140‑1 (MQ=255)
       tGCAGGATATGATCCATATCTTCCGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTGtttttttt                                                                                                                         >  2:1775991/1‑140 (MQ=255)
       tGCAGGATATGATCCATATCTTCCGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCAttttttt                                                                                                                         <  2:3243786/140‑1 (MQ=255)
       tGCAGGATATGATCCATATCTTCCGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTAAatttttt                                                                                                                         >  2:1080382/1‑140 (MQ=255)
       tGCAGGATATGATCCATATCTTCCGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGGTTGAttttttt                                                                                                                         >  1:3961006/1‑140 (MQ=255)
                 gATCCATATCTTCCGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTTATTTTTTTGATGATGGCg                                                                                                               >  1:2448133/1‑140 (MQ=255)
                 gATCCATATCTTCCGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGGTTGATTTTTTTTAgtttgggg                                                                                                               >  1:3828001/1‑133 (MQ=255)
                   tCCATATCTTCCGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGa                                                                                                            <  1:3648371/140‑1 (MQ=255)
                      atatCTTCCGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCTTTTTTCATTTTTTTGTGGATGGCGGAACg                                                                                                         >  2:2531404/1‑140 (MQ=255)
                      atatCTTCCGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACg                                                                                                         >  2:637605/1‑140 (MQ=255)
                      atatCTTCCGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTAAATTTTTTAGGGATGGCGGTACg                                                                                                         >  2:1024354/1‑140 (MQ=255)
                      atatCTTCCGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGGTTGATTTTTTTGAGGATTGGGGCACg                                                                                                         >  1:856666/1‑140 (MQ=255)
                      atatCTTCCGGGAAAAGAGATACATAGTGTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTAATTTTTTTCAGGATGGCGGaaag                                                                                                         >  2:1900244/1‑138 (MQ=255)
                       tatCTTCCGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTGATTTTTTTGATGATTGGGGAACgg                                                                                                        >  1:965061/1‑140 (MQ=255)
                        atCTTCCGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTGTTTTTTTTGTGGATGGGGGGAccgg                                                                                                       >  1:2073532/1‑137 (MQ=255)
                             ccGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGAtc                                                                                                  <  1:182263/140‑1 (MQ=255)
                             ccGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTGATTTTTTTTAGGATGGGGGGACGggtgttg                                                                                                  >  1:931534/1‑137 (MQ=255)
                             ccGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTGATTTTTTTTAGGATGGCGGCACGgattttg                                                                                                  >  1:3180585/1‑136 (MQ=255)
                                            cATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCAttt                                                                                  <  2:3377186/140‑1 (MQ=255)
                                            cATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCAttt                                                                                  <  2:680519/140‑1 (MQ=255)
                                            cATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCAttt                                                                                  <  2:598377/140‑1 (MQ=255)
                                            cATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCAttt                                                                                  <  2:626995/140‑1 (MQ=255)
                                                 ttttGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTGATTTTTTTTAGGATTGCGGGACGGAATATATAGATTTATTAAttttggaa                                                                             >  2:2601417/1‑135 (MQ=255)
                                                   ttGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTGATTTTTTTGAGGGTGGCGGTACGGATGTTCTGGATTTGTTCATTTggggaaa                                                                           >  2:3622319/1‑135 (MQ=255)
                                                    tGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTGATTTTTTTTAGGGTGGCGGAACCGATGATATCAATTTATTAATTTTGCaaaaa                                                                          >  1:4169909/1‑139 (MQ=255)
                                                      cGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCAATTTTTTAAGGGGTGGGGTACGGATTTTAGAGATTTGTTCAGTTTGGCaaataa                                                                         >  2:2017339/1‑137 (MQ=255)
                                                       gTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATc                                                                       <  2:1607090/140‑1 (MQ=255)
                                                         tCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAg                                                                     <  1:1021730/140‑1 (MQ=255)
                                                                  tttACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTGTTTTTTTTGAGGATGGCGGCACGGAGGATATCGATTTGATATATTGGGAAAATATCAGCCGTttcag                                                            >  1:1382110/1‑139 (MQ=255)
                                                                   ttACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTttcatt                                                           <  1:989373/140‑1 (MQ=255)
                                                                   ttACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTttcatt                                                           <  1:61130/140‑1 (MQ=255)
                                                                     aCAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTTATTTTTTTTAGGTTTGGGGAAAGGATTATCTGGATTTATTGATTTTTAAAAATATAATCCTTttgattga                                                         >  2:2929060/1‑138 (MQ=255)
                                                                      cAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGAGGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAg                                                        <  2:1676304/140‑1 (MQ=255)
                                                                            atagcgataTGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTGATTTTTTTCAGGCTGGCGGAACGGATTATCTCGATTTATTAATTTGGCAAAATATCAACCTTTTAATTAAGAAAAta                                                  >  2:3117270/1‑139 (MQ=255)
                                                                            atagcgataTGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTTATTTTTTTAAGGCTTGGGGGAAGGATGATATCGATTTGTTCATTTTTGGAAAAATAAGACTTTTGATTGAGAAAAta                                                  >  1:3053691/1‑139 (MQ=255)
                                                                            atagcgataTGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTGATTTTTTTTATGATGGCGGAACGGATGATAAAGATTTATTCATTTTTGAAAATATTAACCTTTTAAATAAGaaaaaa                                                  >  2:4103912/1‑136 (MQ=255)
                                                                            atagcgataTGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTGATTTTTTTAAGGTTGGCGGAAAGGATTATGTATATTTGTTCGTTTTTCAAAAAATCAGCGGTTTCATTTAGCACAta                                                  >  1:3094592/1‑139 (MQ=255)
                                                                            atagcgataTGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTCATTTTTTTGATGATGGCGGCACCGATGGTCTCGCTTTGTTCATTTTGGAAAAAATCAGCCATTTAAGTCacaacaaa                                                  >  2:3796987/1‑138 (MQ=255)
                                                                            atagcgataTGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTCATTTTTTTAATGATTGCGGAACGGATTTTATCGATTTGTTAATTTTGGAAAAAATAAGCCATTTGTTTAAGAAAAta                                                  >  2:4042788/1‑139 (MQ=255)
                                                                                   tatGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTGATTTTTTTGAGGATGGCGGAAACGATGTTGTCGATTTGTTAATTTTGCAAAATATAATCCATTTAATTAAGAACATCAAtctac                                           >  2:3638370/1‑138 (MQ=255)
                                                                                     tGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGt                                         <  1:840413/140‑1 (MQ=255)
                                                                                     tGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGt                                         <  1:1936334/140‑1 (MQ=255)
                                                                                     tGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGt                                         <  1:3475243/140‑1 (MQ=255)
                                                                                     tGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTAAATTTTTTCATGATGGCGGAAAGGATTATATAGATTTATTAATTTTGCAAAATATAACCCTTTTTATTCATAAAAACaaaaaacag                                         >  2:3152349/1‑133 (MQ=255)
                                                                                          gatgaGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTGAATTTTTTCATGATGGCGGAACCGATGATCTAGATTTGATTATTTGGGCAAATAAAATCCGTTTCAATAAGAAAAAAAATAGCCGTTtatc                                    >  2:4073636/1‑140 (MQ=255)
                                                                                          gatgaGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTGATTTTTTTAAGGATGGCGGAACGGGTGATCTAGATTTGTTGATTTGGGGAAAAATAAGCCATTTGATTAAGAACATAAAAGTCCGgttgag                                    >  2:2380455/1‑137 (MQ=255)
                                                                                             gaGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTCAATTTTTTCAGGTTGGCGGAACCGAATATCTCGCTTTATTAATTTTGGAAAATATCACCCTTTTCATTCAGAACATAAATATACGTTTGTCttt                                 >  2:4318797/1‑140 (MQ=255)
                                                                                                gTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCAt                              <  1:4103912/140‑1 (MQ=255)
                                                                                                gTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCAt                              <  2:2179072/140‑1 (MQ=255)
                                                                                                gTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCAt                              <  2:54614/140‑1 (MQ=255)
                                                                                                gTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCAt                              <  1:431186/140‑1 (MQ=255)
                                                                                                 ttATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTGAGGGTGGGGGAAACGGAGTTCTAGATTTCTTCAATTTGCAAAATATCAGCCGTTTCATTAAGAACAACAAACACCGTGTGACGTTCATc                             >  2:2077175/1‑140 (MQ=255)
                                                                                                  tATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCg                            <  2:1600970/140‑1 (MQ=255)
                                                                                                  tATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCg                            <  2:2432876/140‑1 (MQ=255)
                                                                                                    ttCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGAc                          <  2:3010205/140‑1 (MQ=255)
                                                                                                     tCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACa                         <  2:2609598/140‑1 (MQ=255)
                                                                                                     tCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACa                         <  1:42567/140‑1 (MQ=255)
                                                                                                     tCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACa                         <  1:637605/140‑1 (MQ=255)
                                                                                                     tCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACa                         <  2:882774/140‑1 (MQ=255)
                                                                                                        gTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTGATTTTTTTTAGGCTGGGGGAACGGTGTATCTAGCTTTTTTGCTTTTGCAATATATCAGCCTTTTGATTCAGAACAAAAATCAACGTTTGTCTTTCATCGCCAATg                      >  2:3935147/1‑140 (MQ=255)
                                                                                                         tCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTGATTTTTTTGAGGGTGGCGGGAACGATGATCTCGATTTTTTGATTTTGGCAAAAATAAGCCGTTTGATTGAGAACAACAAAAGCCGGTTGTGGTTGATCGACAAAGt                     >  2:2693377/1‑140 (MQ=255)
                                                                                                         tCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTGAATTTTTTGAGGTTTGGGGGACGGCTGTTATGGGTTTATTGATTTTGGGAAAAATCATCCGTTTGATTTAGAAAAACAAAATCCGATTGTGTTTTATAGTCaaatt                     >  2:2293070/1‑137 (MQ=255)
                                                                                                          cccAAGTTGGACAGAGGGATGCGGTCGTTTTTGATTTTTTTGAGGGTGGCGGCACGGCTGATCTCGATTTCTTGATTTTGGCAAATATAAGCCGTTTCATTCAGAACATCAATCGCCGTTTGGCTTTGATCGACAATGTa                    >  2:4273881/1‑140 (MQ=255)
                                                                                                          cccAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTTTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTa                    <  1:2038276/140‑1 (MQ=255)
                                                                                                          cccAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTa                    <  1:3279282/140‑1 (MQ=255)
                                                                                                          cccAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTa                    <  1:1320018/140‑1 (MQ=255)
                                                                                                          cccAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTa                    <  1:2824516/140‑1 (MQ=255)
                                                                                                          cccAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTa                    <  1:2246379/140‑1 (MQ=255)
                                                                                                          cccAAGTTGGACAGAGGGAGGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTa                    <  1:2306572/140‑1 (MQ=255)
                                                                                                          cccAAGAGGGACAGAGGGATGCGGTCGTTGTTCATTGTTTTCAGGATGCCGGAACGGATGATCTCGCTTTCTTCTTTTGGCACAATATCACCCCTTTCATTCAGAACATCAATCTCCGTGTCTCTTTCATCGACAATGTa                    <  2:3441274/140‑1 (MQ=255)
                                                                                                          accAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTa                    <  2:2355531/139‑1 (MQ=255)
                                                                                                            cAATTTGGACAGATGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACt                  <  2:3699091/140‑1 (MQ=255)
                                                                                                            cAAGTTGGACAGTGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATCTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACt                  <  2:420706/140‑1 (MQ=255)
                                                                                                            cAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACACTGGACt                  <  2:943674/140‑1 (MQ=255)
                                                                                                            cAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACt                  <  1:1169871/140‑1 (MQ=255)
                                                                                                            cAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACt                  <  2:2073532/140‑1 (MQ=255)
                                                                                                            cAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACt                  <  1:1486733/140‑1 (MQ=255)
                                                                                                             aaGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTc                 <  2:274066/140‑1 (MQ=255)
                                                                                                             aaGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTc                 <  2:1297982/140‑1 (MQ=255)
                                                                                                             aaGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTc                 <  1:2562139/140‑1 (MQ=255)
                                                                                                               gtgggACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCg               <  2:3268054/137‑1 (MQ=255)
                                                                                                               gTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCg               <  1:2894434/140‑1 (MQ=255)
                                                                                                               gTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCg               <  1:1819024/140‑1 (MQ=255)
                                                                                                               gTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCg               <  1:2601417/140‑1 (MQ=255)
                                                                                                               gTTGAACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCg               <  1:1130993/140‑1 (MQ=255)
                                                                                                                     cAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATAc         <  2:3210134/140‑1 (MQ=255)
                                                                                                                     cAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATAc         <  2:1928958/140‑1 (MQ=255)
                                                                                                                     cAGAGGGATGCGGTCGTTTTTCATTTTTGTCCGCATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATAc         <  2:1149389/140‑1 (MQ=255)
                                                                                                                     cAGAGGGATGCGGTCGGTGTTCATTGTTTTCAGGAGGGCGGAACGGATGATCTCGATTTCTTCACTGGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATAc         <  1:3388351/140‑1 (MQ=255)
                                                                                                                        agGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGt      <  1:2693377/140‑1 (MQ=255)
                                                                                                                        agGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGt      <  1:111981/140‑1 (MQ=255)
                                                                                                                          ggATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTAc    <  2:1152880/140‑1 (MQ=255)
                                                                                                                          ggATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTAc    <  2:1478225/140‑1 (MQ=255)
                                                                                                                          ggATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTAc    <  1:1356668/140‑1 (MQ=255)
                                                                                                                          ggATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTAc    <  2:3086530/140‑1 (MQ=255)
                                                                                                                          ggATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTAc    <  2:856968/140‑1 (MQ=255)
                                                                                                                          agATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTAc    <  1:2224378/139‑1 (MQ=255)
                                                                                                                            aTGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCt  <  2:1229222/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                   
TTTTTGATGCAGGATATGATCCATATCTTCCGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCT  >  NZ_CP034943/3790630‑3790893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: