Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,993,028 G→A 6.4% S211L (TCG→TTG)  walK ← cell wall metabolism sensor histidine kinase WalK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,993,0280GA 6.4% 1255.4 / 47.8 375S211L (TCG→TTG) walKcell wall metabolism sensor histidine kinase WalK
Reads supporting (aligned to +/- strand):  ref base G (125/226);  new base A (8/16);  total (133/242)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 378 total aligned reads displayed.

ATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAACCTGTGCCGGATGCCAGTATCGTGTTGATCGTCTTCATTTGGTTAAAGACATCTTCCATACTCGCGAC  >  NZ_CP034943/3992890‑3993161
                                                                                                                                          |                                                                                                                                     
aTCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGa                                                                                                                                      >  2:91118/1‑140 (MQ=255)
aTCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGa                                                                                                                                      >  2:4078191/1‑140 (MQ=255)
aTCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGa                                                                                                                                      >  1:3047864/1‑140 (MQ=255)
aTCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGa                                                                                                                                      >  1:2997793/1‑140 (MQ=255)
     cTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCAAAAGCg                                                                                                                                 <  1:2080985/140‑1 (MQ=255)
      tGGGCATCTTCAAGCTCTCTCTTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACATTCCACGAGAAATTACCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCgg                                                                                                                                <  2:1100394/140‑1 (MQ=255)
      tGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGCGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCgg                                                                                                                                <  2:1483862/140‑1 (MQ=255)
      tGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCgg                                                                                                                                <  2:2223167/140‑1 (MQ=255)
      tGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCgg                                                                                                                                <  2:4156384/140‑1 (MQ=255)
      tGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCgg                                                                                                                                <  1:4078191/140‑1 (MQ=255)
      tGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCgg                                                                                                                                <  2:4223086/140‑1 (MQ=255)
      tGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCgg                                                                                                                                <  2:652563/140‑1 (MQ=255)
      tGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCgg                                                                                                                                <  1:2636208/140‑1 (MQ=255)
      tGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCgg                                                                                                                                <  2:787076/140‑1 (MQ=255)
      tGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCAAAAGCgg                                                                                                                                <  1:1714657/140‑1 (MQ=255)
      tGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCAGGCCCGTACTTTTTACCCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCgg                                                                                                                                <  1:3693998/140‑1 (MQ=255)
       gggCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGa                                                                                                                               <  1:1428274/140‑1 (MQ=255)
        ggCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGag                                                                                                                              >  1:3507825/1‑139 (MQ=255)
        ggCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGAt                                                                                                                              >  2:1421475/1‑140 (MQ=255)
        ggCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGAt                                                                                                                              >  1:1264626/1‑140 (MQ=255)
         gCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATg                                                                                                                             <  2:765909/140‑1 (MQ=255)
              ttCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTaa                                                                                                                        <  2:547678/140‑1 (MQ=255)
              ttCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTaa                                                                                                                        <  1:1568348/140‑1 (MQ=255)
              ttCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTaa                                                                                                                        <  2:2732969/140‑1 (MQ=255)
              ttCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACGTTTTAACCGTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTaa                                                                                                                        <  2:4288878/140‑1 (MQ=255)
                       tctcGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCgg                                                                                                                                >  1:1798920/1‑123 (MQ=255)
                       tctcGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTg                                                                                                               >  2:75051/1‑140 (MQ=255)
                       tctcGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCGCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTg                                                                                                               >  2:2945999/1‑140 (MQ=255)
                       tctcGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCAAGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCACGCTCCATCGCCTGTTTTCTCATATCCGACAGCGGATCGGTAATGGTTCTTg                                                                                                               >  2:2319526/1‑140 (MQ=255)
                       tctcGTTAAGTGATTAAACGTAGTGGCAACCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTg                                                                                                               >  1:2134761/1‑140 (MQ=255)
                        ctcGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCgg                                                                                                                                >  2:1818088/1‑122 (MQ=255)
                        ctcGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGc                                                                                                              <  1:1003079/140‑1 (MQ=255)
                        ctcGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCAAAAGCGGATGGGTAATGGTTCTTGc                                                                                                              <  2:1864694/140‑1 (MQ=255)
                        ctcGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCATTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGc                                                                                                              >  1:3477282/1‑140 (MQ=255)
                          cGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCa                                                                                                            <  1:4304878/140‑1 (MQ=255)
                                  gATTACCCGTTGTGGCAAGCTGCCCAATTTCATCCTGCCCGAACTTTTTAACCGTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATa                                                                                                    <  1:2227582/140‑1 (MQ=255)
                                  gATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATa                                                                                                    <  2:3624742/140‑1 (MQ=255)
                                  gATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATa                                                                                                    <  2:3966659/140‑1 (MQ=255)
                                  gATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCAAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATa                                                                                                    <  1:919426/140‑1 (MQ=255)
                                   aTTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATAc                                                                                                   >  1:754049/1‑140 (MQ=255)
                                           tAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAgg                                                                                           <  2:2105384/140‑1 (MQ=255)
                                             gTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGc                                                                                         >  2:3265974/1‑140 (MQ=255)
                                                   aGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCaa                                                                                   >  2:1979031/1‑140 (MQ=255)
                                                       gCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACa                                                                               <  1:2559038/140‑1 (MQ=255)
                                                       gCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACa                                                                               <  1:1979031/140‑1 (MQ=255)
                                                       gCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACa                                                                               <  2:842830/140‑1 (MQ=255)
                                                       gCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACa                                                                               <  1:2886511/140‑1 (MQ=255)
                                                       gCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCAGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACa                                                                               <  1:855621/140‑1 (MQ=255)
                                                       gCCCAATTTCATCATCCCCGTACTTTATAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACa                                                                               <  1:2797665/140‑1 (MQ=255)
                                                        cccAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCCGAAAAATACCGAGAAGGGCCGTCAAAACaa                                                                              >  1:473260/1‑140 (MQ=255)
                                                        cccAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCCGAAAAATACCGAGAAGGGCCGTCAAAACaa                                                                              >  2:2278654/1‑140 (MQ=255)
                                                        cccAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACac                                                                              >  1:2293152/1‑139 (MQ=255)
                                                        cccAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACaa                                                                              >  2:3911922/1‑140 (MQ=255)
                                                            aTTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGc                                                                          <  1:3522707/140‑1 (MQ=255)
                                                            aTTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGc                                                                          <  1:3950126/140‑1 (MQ=255)
                                                                catcatGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCaaa                                                                      >  1:2475954/1‑140 (MQ=255)
                                                                  tcatGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAAcc                                                                    >  1:3879579/1‑140 (MQ=255)
                                                                  tcatGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAAcc                                                                    >  1:629083/1‑140 (MQ=255)
                                                                  tcatGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAAcc                                                                    >  1:3248152/1‑140 (MQ=255)
                                                                       cccGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATg                                                                                                                             >  2:3743572/1‑78 (MQ=255)
                                                                       cccGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATg                                                                                                                             >  2:1597181/1‑78 (MQ=255)
                                                                       cccGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATg                                                                                                                             <  1:3743572/78‑1 (MQ=255)
                                                                         cGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAGAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAACCTGTGCCg                                                             <  1:4327741/140‑1 (MQ=255)
                                                                         cGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAACCTGTGCCg                                                             <  1:1843930/140‑1 (MQ=255)
                                                                         cGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAACCTGTGCCg                                                             <  1:715419/140‑1 (MQ=255)
                                                                         cGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAACCTGTGCCg                                                             <  1:1621375/140‑1 (MQ=255)
                                                                              tttttAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAACCTGTGCCGGATGc                                                        <  1:3164607/140‑1 (MQ=255)
                                                                                tttAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAACCTGTGCCGGATGCCa                                                      >  1:3355146/1‑140 (MQ=255)
                                                                                    aCCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAACCTGTGCCGGATGCCAGTAt                                                  <  1:798091/140‑1 (MQ=255)
                                                                                        tCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCCAAACACGTGCCACACCTGGGCCGGATGCCAGTATCgtg                                              >  2:439614/1‑140 (MQ=255)
                                                                                        tCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAACCTGTGCCGGATGCCAGTa                                                   <  1:1064022/135‑1 (MQ=255)
                                                                                        tCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAACCTGTGCCGGATGCCAGTa                                                   >  2:3679494/1‑135 (MQ=255)
                                                                                        tCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAACCTGTGCCGGATGCCAGTATCgtg                                              >  1:415350/1‑140 (MQ=255)
                                                                                        tCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAACCTGTGCCGGATGCCAGTATCgtg                                              >  1:518609/1‑140 (MQ=255)
                                                                                            cGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCACAACAAGTGCCAAACCTGTGCCGGATGCCAGTATCGTGTTGa                                          <  1:2898545/140‑1 (MQ=255)
                                                                                            cGAGAAAGTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAACCTGTGCCGGATGCCAGTATCGTGTTGa                                          <  1:1347794/140‑1 (MQ=255)
                                                                                                  aTTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAACCTGTGCCGGATGCCAGTATCGTGTTGATCGTCt                                    <  1:3844355/140‑1 (MQ=255)
                                                                                                  aTTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAACCTGTGCCGGATGCCAGTATCGTGTTGATCGTCt                                    <  1:4240063/140‑1 (MQ=255)
                                                                                                       cTTGCGCAAGCGCCAGCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAACCTGTGCCGGATGCCAGTATCGTGTTGATCGTCTTCAtt                               <  2:3485007/140‑1 (MQ=255)
                                                                                                           cGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAACCTGTGCCGGATGCCAGTATCGTGTTGATCGTCTTCATTTGGt                           >  2:1324460/1‑140 (MQ=255)
                                                                                                           cGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAACCTGTGACGGATGCCAGTATCGTGTTGATCGTCTTCATTTGGt                           <  2:3310832/140‑1 (MQ=255)
                                                                                                                gCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAACCTGTGCCGGATGCCAGTATCGTGTTGATCGTCTTCATTTGGTTAAAg                      >  2:2994458/1‑140 (MQ=255)
                                                                                                                  tCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAACCTGTGCCGGATGCCAGTATCGTGTTGATCGTCTTCATTTGGTTAAAGAc                    <  2:3894205/140‑1 (MQ=255)
                                                                                                                  tCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAACCTGTGCCGGATGCCAGTATCGTGTTGATCGTCTTCATTTGGTTAAAGAc                    <  1:2923448/140‑1 (MQ=255)
                                                                                                                  tCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAACCTGTGCCGGATGCCAGTATCGTGTTGATCGTCTTCATTTGGTTAAAGAc                    <  2:2036303/140‑1 (MQ=255)
                                                                                                                  tCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAACCTGTGCCGGATGCCAGTATCGTGTTGATCGTCTTCATTTGGTTAAAGAc                    <  2:3199867/140‑1 (MQ=255)
                                                                                                                  tCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAACCTGTGCCGGATGCCAGTATCGTGTTGATCGTCTTCATTTGGTTAAAGAc                    <  2:1627343/140‑1 (MQ=255)
                                                                                                                  tCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAACCTGTGCCGGATGCCAGTATCGTGTTGATCGTCTTCATTTGGTTAAAGAc                    <  2:243570/140‑1 (MQ=255)
                                                                                                                  tCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAACCTGTGCCGGATGCCAGTATCGTGTTGATCGTCTTCATTTGGTTAAAGAc                    <  2:177614/140‑1 (MQ=255)
                                                                                                                  tCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAACCGGTGCCGGATGCCAGTATCGTGTTGATCGTCTTCATTTGGTTAAAGAc                    <  1:662938/140‑1 (MQ=255)
                                                                                                                  tCCATCGCCTGTTTTCTCATATCCAAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAACCTGTGCCGGATGCCAGTATCGTGTTGATCGTCTTCATTTGGTTAAAGAc                    <  2:707604/140‑1 (MQ=255)
                                                                                                                     aTCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAACCTGTGCCGGATGCCAGTAGCGTGTTGATCGTCTTCATTTGGTTAAAGACATc                 >  2:2576128/1‑140 (MQ=255)
                                                                                                                      tCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAACCTGTGCCGGATGCCAGTATCGTGTTGATCGTCTTCATTTGGTTAAAGACATCt                >  2:1039885/1‑140 (MQ=255)
                                                                                                                         ccTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAACCTGTGCCGGATGCCAGTATCGTGTTGATCGTCTTCATTTGGTTAAAGACATCTTcc             >  1:2436372/1‑140 (MQ=255)
                                                                                                                              tttCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAACCTGTGCCGGATGCCAGTATCGTGTTGATCGTCTTCATTTGGTTAAAGACATCTTCCATACt        <  1:2352415/140‑1 (MQ=255)
                                                                                                                              tttCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAACCTGTGCCGGATGCCAGTATCGTGTTGATCGTCTTCATTTGGTTAAAGACATCTTCCATACt        <  1:1201614/140‑1 (MQ=255)
                                                                                                                                tctcATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAACCTGTGCCGGATGCCAGTATCGTGTTGATCGTCTTCATTTGGTTAAAGACATCTTCCATACTcg      <  1:977851/140‑1 (MQ=255)
                                                                                                                                  tcATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAACCTGTGCCGGATGCCAGTATCGTGTTGATCGTCTTCATTTGGTTAAAGACATCTTCCATACTcgcg    <  2:1350729/140‑1 (MQ=255)
                                                                                                                                   cATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAACCTGTGCCGGATGCCAGTATCGTGTTGATCGTCTTCATTTGGTTAAAGACATCTTCCATACTCGCGa   <  2:3047864/140‑1 (MQ=255)
                                                                                                                                    atatCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAACCTGTGCCGGATGCCAGTATCGTGTTGATCGTCTTCATTTGGTTAAAGACATCTTCCATACTCGCGac  >  1:3645528/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                     
ATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTCATATCCGAAAGCGGATGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGGGCCGTCAAAACAAGTGCCAAACCTGTGCCGGATGCCAGTATCGTGTTGATCGTCTTCATTTGGTTAAAGACATCTTCCATACTCGCGAC  >  NZ_CP034943/3992890‑3993161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: