Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,994,110 C→G 6.0% V89L (GTC→CTC)  walR ← cell wall metabolism DNA‑binding response regulator WalR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,994,1100CG 6.0% 1346.7 / 56.9 421V89L (GTC→CTC) walRcell wall metabolism DNA‑binding response regulator WalR
Reads supporting (aligned to +/- strand):  ref base C (180/214);  new base G (11/15);  total (191/230)
Fisher's exact test for biased strand distribution p-value = 8.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 422 total aligned reads displayed.

ATTAGAGGACGGCTCTTCCTCCGCAGGCGCTGTTGTCAGCTGGCGGCGCAAGTTCGCTTTTACACGTGCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAACACCGTCTTTATTAGGAAGCATAATATCTAAAAGAATTAAATCAGGCTGAAGCTCTTCT  >  NZ_CP034943/3993976‑3994249
                                                                                                                                      |                                                                                                                                           
   agagGACGGCTCTTCCTCCGCAGGCGCTGTTGTCAGCTGGCGGCGCAAGTTCGCTTTTACACGTGCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCaa                                                                                                                                     <  1:2415140/140‑1 (MQ=255)
   agagGACGGCTCTTCCTCCGAAGGAGCTGTTGTCAGCTGGCGGCGCAAGTTCGCTTTTACACGTGCCAGGAGCTCGCGTGTGCTGTATGGTTTTGTAACATAGTCATCAGCACAGATTTCAAGCCCGATGACCTTTTCaa                                                                                                                                     <  1:3128446/140‑1 (MQ=255)
     agGACGGCTCTTCCTCCGCAGGCGCTGTTGTCAGCTGGCGGCGCAAGTTCGCTTTTACACGTGCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAAtt                                                                                                                                   >  2:3164893/1‑140 (MQ=255)
        aCGGCTCTTCCTCCGCAGGCGCTGTTGTCAGCTGGCGGCGCAAGTTCGCTTTTACACGTGCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCt                                                                                                                                >  2:2234619/1‑140 (MQ=255)
        aCGGCTCTTCCTCCGCAGGCGCTGTTGTCAGCTGGCGGCGCAAGTTCGCTTTTACACGTGCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCt                                                                                                                                >  2:3030461/1‑140 (MQ=255)
          ggCTCTTCCTCCGCAGGCGCTGTTGTCAGCTGGCGGCGCACGTTCGCTTTTACACGTGCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGa                                                                                                                              >  1:998483/1‑140 (MQ=255)
          ggCTCTTCCTCCGCAGGCGCTGTTGTCAGCTGGCGGCGCAAGTTCGCTTTTACACGTGCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGa                                                                                                                              >  1:1092011/1‑140 (MQ=255)
          ggCTCTTCCTCCGCAGGCGCTGTTGTCAGCTGGCGGCGCAAGTTCGCTTTTACACGTGCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGa                                                                                                                              >  1:2242817/1‑140 (MQ=255)
            ctctTCCTCCGCAGGCGCTGTTGTCAGCTGGCGGCGCAAGTTCGCTTTTACACGTGCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAAt                                                                                                                            >  1:849248/1‑140 (MQ=255)
                    ccGCAGGCGCTGTTGTCAGCTGGCGGCGCAAGTTCGCTTTTACACGTGCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGcc                                                                                                                    >  1:3612031/1‑140 (MQ=255)
                      gCAGGCGCTGTTGTCAGCTGGCGGCGCAAGTTCGCTTTTACACGTGCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGt                                                                                                                  <  2:1048782/140‑1 (MQ=255)
                      gCAGGCGCTGTTGTCAGCTGGCGGCGCAAGTTCGCTTTTACACGTGCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGt                                                                                                                  <  1:3347172/140‑1 (MQ=255)
                        aGGCGCTGTGGTCAGCTGGCGGCGCAAGTTCGCTTTTACACGCGCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGAGTCCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCa                                                                                                                <  2:2385809/140‑1 (MQ=255)
                         ggCGCTGTTGTCAGCTGGCGGCGCAAGTTCGCTTTTACACGTGCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAg                                                                                                               >  2:1651043/1‑140 (MQ=255)
                                  gtCAGCTGGCGTCGCAAGTTCGCTTTTACACGTGCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGAt                                                                                                      <  2:83200/140‑1 (MQ=255)
                                  gtCAGCTGGCGGCGCAAGTTCGCTTTTACACGTGCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGAt                                                                                                      <  1:2342878/140‑1 (MQ=255)
                                  gtCAGCTGGCGGCGCAAGTTCGCTTTTACACGTGCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGAt                                                                                                      <  2:3679674/140‑1 (MQ=255)
                                  gtCAGCTGGCGGCGCAAGTTCGATTTTACAAGTGCGAGGAGCTCGCGTGTTCTGAATGTTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGAt                                                                                                      <  1:3225195/140‑1 (MQ=255)
                                       cTGGCGGCGCAAGTTCGCTTTTACACGTGCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGAGCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCa                                                                                                 <  2:2113419/140‑1 (MQ=255)
                                         ggcggcGCAAGTTCGCTTTTACACGTGCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCata                                                                                               <  2:2501708/140‑1 (MQ=255)
                                          gcggcgCAAGTTCGCTTTTCCACGTGCCAGGAGCTCGCGTGTGCTGAAGGGTTTTGTAACATAGGCATCAGAACCGATTTCACGCCCGATGCGCTTGTGAATTTCTGAATCCGTCGCCGTCAGCATAATGATCGGCatat                                                                                              >  1:381808/1‑140 (MQ=255)
                                          gcggcgCAAGTTCGCTTTTACACGTGCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCatat                                                                                              >  1:1297919/1‑140 (MQ=255)
                                               gcAGGTTCGCTTTTACACGCGCCAAGAGCTCGCGTGTGCTGAATGGTTTTGTGACATAGTCATCAGCACCGATTTCAAGCCCGATAACCTTGTCAATTTCTGAATCCTTAGCCGTCAGCATAATGATTGGCATATCGTAt                                                                                         <  2:2932207/140‑1 (MQ=255)
                                               gcAGGTTCGCTTTTACACGCGCCAAGAGCTCGCGTGTGCTGAATGGTTTTGTGACATAGTCATCAGCACCGATTTCAAGCCCGATAACCTTGTCAATTTCTGAATCCTTAGCCGTCAGCATAATGATTGGCATATCGTAt                                                                                         <  2:1279640/140‑1 (MQ=255)
                                               gcAAGTTCGCTTTTACACGTGCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTAt                                                                                         <  2:311048/140‑1 (MQ=255)
                                               gcAAGTTCGCTTTTACACGTGCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTAt                                                                                         <  2:430494/140‑1 (MQ=255)
                                               gcAAGTTCGCTTTTACACGTGCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACAGAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTAt                                                                                         <  1:1609156/140‑1 (MQ=255)
                                                     tCGCTTTTACACGTGCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTAtttcttt                                                                                   <  2:2558850/140‑1 (MQ=255)
                                                       gCTTTTACACGTGCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATttctttct                                                                                 <  1:2795279/140‑1 (MQ=255)
                                                       gCTTTTACACGTGCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATttctttct                                                                                 <  1:100247/140‑1 (MQ=255)
                                                           ttACACGTGCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACt                                                                             <  2:3797072/140‑1 (MQ=255)
                                                                 gTGCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATACTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGAGCGGCATCTCGTATTTCTTTCTGACTTCACgg                                                                       <  2:1121891/140‑1 (MQ=255)
                                                                   gCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCATCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCa                                                                     <  1:2485586/140‑1 (MQ=255)
                                                                   gCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCa                                                                     >  2:1496520/1‑140 (MQ=255)
                                                                   gCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCa                                                                     <  2:3889670/140‑1 (MQ=255)
                                                                   gCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCa                                                                     <  2:1213143/140‑1 (MQ=255)
                                                                   gCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCa                                                                     >  1:4201109/1‑140 (MQ=255)
                                                                   gCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCa                                                                     <  1:472454/140‑1 (MQ=255)
                                                                   gCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCa                                                                     <  2:1747832/140‑1 (MQ=255)
                                                                   gCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCa                                                                     >  1:3288858/1‑140 (MQ=255)
                                                                   gCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCa                                                                     <  1:314684/140‑1 (MQ=255)
                                                                   gCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCa                                                                     <  1:1421835/140‑1 (MQ=255)
                                                                   gCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCa                                                                     <  1:2973226/140‑1 (MQ=255)
                                                                   gCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCa                                                                     <  2:3551412/140‑1 (MQ=255)
                                                                   gCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCa                                                                     <  1:228619/140‑1 (MQ=255)
                                                                   gCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCa                                                                     <  1:1859616/140‑1 (MQ=255)
                                                                   gCCAGGAGCTCCCGTGTGCTGAATGGTTTTGTTACATCGTCATCACCACCGATTTCAAGCCCGATGAGCTTGTCAATTTCTGACTCCTTCGCCGTCATCATAATGATCGGCATATCGTATTTCTTTCTGAATTCACGGCa                                                                     <  2:3733731/140‑1 (MQ=255)
                                                                           cTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCaa                                                             >  1:3763483/1‑140 (MQ=255)
                                                                           cTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCaa                                                             >  2:4165542/1‑140 (MQ=255)
                                                                            tCGCGTGTGCTGAATGGTTTTTTAACATAGTCATCAGCACCGATTTGAAGCCCGATGACCTTGTAAATTTCTGAATCCTTCGCCGTAAGCATAATGATAGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAac                                                            <  1:1526505/140‑1 (MQ=255)
                                                                             cgcgTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGAGCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCa                                                                          <  1:904703/125‑1 (MQ=255)
                                                                             cgcgTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGAGCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCa                                                                          >  2:904703/1‑125 (MQ=255)
                                                                             cgcgTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGAGCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCa                                                                     <  1:3844357/130‑1 (MQ=255)
                                                                             cgcgTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGa                                                                                                                              <  2:690349/73‑1 (MQ=255)
                                                                             cgcgTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCa                                                                     >  2:2963900/1‑130 (MQ=255)
                                                                             cgcgTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAaca                                                           >  1:816385/1‑140 (MQ=255)
                                                                             cgcgTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAaca                                                           >  2:1084226/1‑140 (MQ=255)
                                                                             cgcgTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAaca                                                           >  2:592876/1‑140 (MQ=255)
                                                                             cgcgTGTGCTGAATGGTGTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAaca                                                           >  2:474988/1‑140 (MQ=255)
                                                                                 tgtgCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAACACCGt                                                       <  1:4082428/140‑1 (MQ=255)
                                                                                   tgCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAACACCGTCt                                                     >  1:511027/1‑140 (MQ=255)
                                                                                   tgCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAACACCGTCt                                                     >  1:374000/1‑140 (MQ=255)
                                                                                        aaTGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAACACCGTCTttatt                                                >  1:1802158/1‑140 (MQ=255)
                                                                                        aaTGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAACACCGTCTttatt                                                >  1:388685/1‑140 (MQ=255)
                                                                                           gtttttGTAACATAGTCATCATCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTAAACACCGTCTTTATTAgg                                             <  1:2749871/138‑1 (MQ=255)
                                                                                           ggTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAACACCGTCTTTATTAgg                                             <  1:282999/140‑1 (MQ=255)
                                                                                           ggTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAACACCGTCTTTATTAgg                                             <  1:2021627/140‑1 (MQ=255)
                                                                                           ggTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAACACCGTCTTTATTAgg                                             <  1:1653847/140‑1 (MQ=255)
                                                                                           ggTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAACACCGTCTTTATTAgg                                             <  1:1244042/140‑1 (MQ=255)
                                                                                           ggTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAACACCGTCTTTATTAgg                                             <  1:569400/140‑1 (MQ=255)
                                                                                           ggTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAACACCGTCTTTATTAgg                                             <  1:77175/140‑1 (MQ=255)
                                                                                             ttttGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAACACCGTCTTTATTAGGaa                                           >  1:1732854/1‑140 (MQ=255)
                                                                                             ttttGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAACACCGTCTTTATTAGGaa                                           >  1:3397318/1‑140 (MQ=255)
                                                                                             ttttGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAACACCGTCTTTATTAGGaa                                           >  1:691095/1‑140 (MQ=255)
                                                                                                tGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATAGTTCAACACCGGCTTTATTAGGAAGCa                                        >  2:1536801/1‑140 (MQ=255)
                                                                                                tGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAACACCGTCTTTATTAGGAAGCa                                        <  2:2674457/140‑1 (MQ=255)
                                                                                                  tAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAACACCGTCTTTATTAGGAAGCata                                      >  1:292551/1‑140 (MQ=255)
                                                                                                  tAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGAATATCGTATTTCTTTATTAATTCACGGGATAATTAAAAAACTTCTTTATTAGGAAgatta                                      <  2:3392184/140‑5 (MQ=255)
                                                                                                       tAGTCATCAGCACCGATTTCAAGCCCGATGAGCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAACACCGTCTTTATTAGGAAGCATAATATc                                 >  1:1173330/1‑140 (MQ=255)
                                                                                                       tAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCaa                                                             >  1:366901/1‑112 (MQ=255)
                                                                                                       tAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAACACCGTCTTTATTAGGAAGCATAATATc                                 >  2:192986/1‑140 (MQ=255)
                                                                                                       tAGTCACCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAACACCGTCTTTATTAGGAAGCATAATATc                                 >  2:806353/1‑140 (MQ=255)
                                                                                                           catcaGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAACACCGTCTTTATTAGGAAGCATAATATCTaaa                             <  1:3534361/140‑1 (MQ=255)
                                                                                                            atcaGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGAt                                                                                                      >  2:2804309/1‑66 (MQ=255)
                                                                                                            atcaGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAACACCGTCTTTATTATGAAGCATAATATCTaaaa                            >  2:778566/1‑140 (MQ=255)
                                                                                                            atcaGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAACACCGTCTTTATTAGGAAGCATAATATCTaaaa                            >  1:3054323/1‑140 (MQ=255)
                                                                                                            atcaGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAACACCGTCTTTATTAGGAAGCATAATATCTaaaa                            >  1:213145/1‑140 (MQ=255)
                                                                                                               aGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAACACCGTCTTTATTAGGAAGCATAATATCTAAAAGaa                         >  2:3239836/1‑140 (MQ=255)
                                                                                                               aGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAACACCGTCTTTATTAGGAAGCATAATATCTAAAAGaa                         >  2:2049724/1‑140 (MQ=255)
                                                                                                               aGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGAATATCGTATTTCTTTCTGACTTCACGGCATACTTCAACACCGTCTTTATTAGGAAGCATAATATCTAAAAGaa                         <  1:3075424/140‑1 (MQ=255)
                                                                                                               aGCACCGATTTCAACCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAACACCCTCTTTCTTAGGAAGCATAATATCTAAAAGaa                         >  2:4132610/1‑140 (MQ=255)
                                                                                                                      aTTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAaca                                                           <  2:3299256/99‑1 (MQ=255)
                                                                                                                      aTTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAaca                                                           >  1:3301693/1‑99 (MQ=255)
                                                                                                                      aTTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAACACCGTCTTTATTAGGAAGCATAATATCTAAAAGAATTAAATc                  >  2:449291/1‑140 (MQ=255)
                                                                                                                      aTTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAACACCGTCTTTATTAGGAAGCATAATATCTAAAAGAATTAAATc                  >  1:630540/1‑140 (MQ=255)
                                                                                                                             gCCCGATGAGCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAACACCGTCTTTATTAGGAAGCATAATATCTAAAAGAATTAAATCAGGCTGa           <  1:3468582/140‑1 (MQ=255)
                                                                                                                             gCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAACACCGTCTTTATTAGGAAGCATAATATCTAAAAGAATTAAATCAGGCTGa           <  2:351943/140‑1 (MQ=255)
                                                                                                                               ccGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAACACCGTCTTTATTAGGAAGCATAATATCTAAAAGAATTAAATCAGGCTGAAg         <  1:4163971/140‑1 (MQ=255)
                                                                                                                                 gATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAACACCGTCTTTATTAGGAAGCATAATATCTAAAAGAATTAAATCAGGCTGAAGct       >  2:4072399/1‑140 (MQ=255)
                                                                                                                                  aTGAGCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCTACACCGTCTTTATTAGGAAGCATAATATCTAAAAGAATTAAATCAGGCTGTAGctc      >  1:263550/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                           
ATTAGAGGACGGCTCTTCCTCCGCAGGCGCTGTTGTCAGCTGGCGGCGCAAGTTCGCTTTTACACGTGCCAGGAGCTCGCGTGTGCTGAATGGTTTTGTAACATAGTCATCAGCACCGATTTCAAGCCCGATGACCTTGTCAATTTCTGAATCCTTCGCCGTCAGCATAATGATCGGCATATCGTATTTCTTTCTGACTTCACGGCATACTTCAACACCGTCTTTATTAGGAAGCATAATATCTAAAAGAATTAAATCAGGCTGAAGCTCTTCT  >  NZ_CP034943/3993976‑3994249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: