Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,996,851 A→T 9.0% intergenic (‑157/+49) purA ← / ← EO946_RS20530 adenylosuccinate synthase/VOC family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,996,8510AT 9.0% 609.9 / 7.2 204intergenic (‑157/+49)purA/EO946_RS20530adenylosuccinate synthase/VOC family protein
Reads supporting (aligned to +/- strand):  ref base A (114/67);  new base T (2/21);  total (116/88)
Fisher's exact test for biased strand distribution p-value = 4.96e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 252 total aligned reads displayed.

AACCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGG  >  NZ_CP034943/3996712‑3996989
                                                                                                                                           |                                                                                                                                          
acccTTTCAAAACGATTCTATACACACACATCAAGTTTATCAGTCAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATAAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                            <  1:2212372/138‑1 (MQ=255)
aaCCTTTCAACACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                            <  2:1486604/140‑1 (MQ=255)
aaCCTTTCAAAACGATTCTATTCAAACAACTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTACATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                            <  2:550866/140‑1 (MQ=255)
aaCCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCCTTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                            <  2:685320/140‑1 (MQ=255)
aaCCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                            <  2:1731133/140‑1 (MQ=255)
aaCCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                            <  1:1200436/140‑1 (MQ=255)
aaCCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                            <  1:2236653/140‑1 (MQ=255)
aaCCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                            <  1:1290364/140‑1 (MQ=255)
aaCCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                            <  2:1266146/140‑1 (MQ=255)
aaCCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                            <  2:1306895/140‑1 (MQ=255)
aaCCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                            <  2:1646043/140‑1 (MQ=255)
aaCCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                            <  1:207348/140‑1 (MQ=255)
aaCCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTCCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                            <  2:73003/140‑1 (MQ=255)
   cTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                            >  1:708189/1‑137 (MQ=255)
   cTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                            <  2:829998/137‑1 (MQ=255)
   cTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                            >  1:829998/1‑137 (MQ=255)
     ttCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAATCGTTTTCTCATCGCATTGTc                                                                                                                                       >  2:2146761/1‑140 (MQ=255)
     ttCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTc                                                                                                                                       >  1:2187228/1‑140 (MQ=255)
      tCAAAACGATTCTATTCAAACAAATCAAGTTTATCCGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTcc                                                                                                                                      >  2:1817015/1‑140 (MQ=255)
      tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAATTTATTGTAAATCTATATTCGTTCGCTTACATTCTTTTTATAAGCGTTTTCTCACTGCTTTGTca                                                                                                                                      >  2:3261116/1‑139 (MQ=255)
      tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATTTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTcc                                                                                                                                      >  2:3712991/1‑140 (MQ=255)
      tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAGTCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGtac                                                                                                                                      >  2:2024297/1‑138 (MQ=255)
      tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACCAGCGTTTTCTCATCGCATTGTca                                                                                                                                      >  2:4077535/1‑139 (MQ=255)
      tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTcc                                                                                                                                      >  2:3167687/1‑140 (MQ=255)
      tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTcc                                                                                                                                      >  1:3145278/1‑140 (MQ=255)
      tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTcc                                                                                                                                      >  1:2946451/1‑140 (MQ=255)
      tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTcc                                                                                                                                      >  2:3879045/1‑140 (MQ=255)
      tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTcc                                                                                                                                      >  2:2196606/1‑140 (MQ=255)
      tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTAGCTTCCATTCGTTTTACATGCGTTTTCTCATCGCATTGTcc                                                                                                                                      >  2:4163795/1‑140 (MQ=255)
      tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAAGTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAATCGTTTTATAATCGCATTGTcc                                                                                                                                      >  2:3526354/1‑140 (MQ=255)
      tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAAGTGTAAATCCGAACATTACATTAATGTAAATCAATATTCGTTCGCGTCCAGTCGTTTTACAAGCGTTTTCTCATCGCATTGtgc                                                                                                                                      >  1:3656706/1‑138 (MQ=255)
      tCAAAACGATTCTATTCAAACAAATCAAGGTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATACGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTGCATTCGTTTTACAAGCGTTTTCTCATCGGATTGTcc                                                                                                                                      >  1:2905090/1‑140 (MQ=255)
               ttCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCt                                                                                                                             >  2:4195836/1‑140 (MQ=255)
                    ttaaaaaaaaTAAAGTTTATCATTGAAGAAACATAAAGTCAATTGTAAATCAGAAAATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTAAAAGGGTTTTATCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                        <  2:3733304/137‑1 (MQ=255)
                    ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                        <  1:179501/140‑1 (MQ=255)
                    ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                        <  1:4195836/140‑1 (MQ=255)
                    ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                        <  2:1108261/140‑1 (MQ=255)
                    ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                        <  1:105133/140‑1 (MQ=255)
                       aaacaaaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAAc                                                                                                                     >  2:2645303/1‑140 (MQ=255)
                         acaaaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCCTTCGTGTTACAAGCGTTTTCTCAGCGCTGTGTGCGAAAAAGCTCGTGAAACtt                                                                                                                   >  2:2495757/1‑140 (MQ=255)
                         acaaaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACtt                                                                                                                   >  2:2143480/1‑140 (MQ=255)
                         acaaaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACtt                                                                                                                   >  1:1250480/1‑140 (MQ=255)
                            aaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                            >  2:1139446/1‑112 (MQ=255)
                              tCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCTTCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATc                                                                                                              >  1:3438696/1‑140 (MQ=255)
                              tCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATc                                                                                                              >  1:1125399/1‑140 (MQ=255)
                              tCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATc                                                                                                              >  1:2915112/1‑140 (MQ=255)
                              tCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATc                                                                                                              >  1:1517927/1‑140 (MQ=255)
                              tCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATc                                                                                                              >  1:3774873/1‑140 (MQ=255)
                              tCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATc                                                                                                              >  1:3810065/1‑140 (MQ=255)
                                     tATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAATCTCGTGAAACTTGTATCCTTTAGc                                                                                                       >  2:2976895/1‑140 (MQ=255)
                                      aTCAGTGAAGAAACAGAAACTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCa                                                                                                      <  2:3054580/140‑1 (MQ=255)
                                       tCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCAc                                                                                                     >  1:4248658/1‑140 (MQ=255)
                                         aGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGa                                                                                                   <  2:628761/140‑1 (MQ=255)
                                                     gAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACt                                                                                       >  1:654837/1‑140 (MQ=255)
                                                     gAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACt                                                                                       >  1:1704814/1‑140 (MQ=255)
                                                     gAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACt                                                                                       >  1:440137/1‑140 (MQ=255)
                                                       aaGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCATTTAGCACGAAAAAGAGTTAcgtc                                                                                     >  2:1954699/1‑137 (MQ=255)
                                                            aaTTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGACAAAGAGTTACTTGCCTGa                                                                                >  1:3025918/1‑140 (MQ=255)
                                                            aaTTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGa                                                                                <  2:2189718/140‑1 (MQ=255)
                                                              ttGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGata                                                                              >  2:844116/1‑140 (MQ=255)
                                                              ttGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGata                                                                              >  2:2332112/1‑140 (MQ=255)
                                                               tGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGatat                                                                             <  1:2650181/140‑1 (MQ=255)
                                                               tGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGatat                                                                             <  1:3167687/140‑1 (MQ=255)
                                                                    aTCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCtt                                                                        >  1:2754163/1‑140 (MQ=255)
                                                                    aTCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCtt                                                                        >  1:522382/1‑140 (MQ=255)
                                                                        gAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGt                                                                    >  2:4002382/1‑140 (MQ=255)
                                                                         aaCATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACCAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAACAAGAGTTACTTGCGTGATATCCCTTTTGTa                                                                   >  1:3607759/1‑140 (MQ=255)
                                                                         aaCATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTa                                                                   >  1:1864765/1‑140 (MQ=255)
                                                                                aattaaTGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATaa                                                            >  1:2378573/1‑140 (MQ=255)
                                                                                aattaaTGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATaa                                                            >  1:397004/1‑140 (MQ=255)
                                                                                aattaaTGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATaa                                                            >  1:1482725/1‑140 (MQ=255)
                                                                                aattaaTGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATaa                                                            >  2:44387/1‑140 (MQ=255)
                                                                                    aaTTTAAATCATTATTCTTTCGCTTCCATTGGTTTTACATTCGTTTTCTCTTCGCATTGTCATAAAAAGTTCTTTAAACTTTTATCCTTTAGCACGAAAAATTGTTACTGGCCTTATTTCCCTTTTGTATTCAAAAacac                                                        <  2:1399985/140‑1 (MQ=255)
                                                                                    aaTGAAAATCAACATTCGTTCGCGTCCAGTGGTTTTACAAGGGTTTTCTCATCTCATTGTCCTAAAAAGCTCGTGAAACTTTTATCATTTAGCACGAAAAAAAGTTACGTGCCTGATAGCCGTTTTGAAGTCATAAAAAc                                                        <  2:2405804/140‑1 (MQ=255)
                                                                                      tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCTCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACTAAAAAGAGTTACTTGCGTGATATCCCTTTTGTATTCATAAAATCGa                                                      >  1:2731208/1‑140 (MQ=255)
                                                                                      tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                      >  1:3351272/1‑140 (MQ=255)
                                                                                      tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                      >  1:3996500/1‑140 (MQ=255)
                                                                                               atatTCTTTCGTTTCAATTCGTTTTAAAAGCGTTTTATCATCGAATTTTAATAAAAAGCTCGTTAAACTTTTATCCTTTAGCACGAAAAAGAGTTAGTTGCCTGATATCCCTTTTTTATTCATAAAAACGACTCGCCAAc                                             <  2:2572639/140‑1 (MQ=255)
                                                                                                    ggttcgCTTCATTTCTTTTTACAAGCTTTTTCTCTTCGATTTTTCCTAAAAAGCTCTTTAAAATTTTATCCTTGAGCACCAAAAATAGTTAGTTGCCTTATATCCTTTTTGTATTCTAAAAAACGACTCGCCAACCGTcc                                        <  1:895373/139‑1 (MQ=255)
                                                                                                    cgttcgCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGa                                                                                >  2:2540182/1‑100 (MQ=255)
                                                                                                    cgttcgCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGa                                                                                <  1:2540173/100‑1 (MQ=255)
                                                                                                          cTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAGCAGTTCCTTGCCTGATATCCCTTTTGTATGAATAAAAACGACACGCCCACCGGCCGGagcg                                  >  1:2564255/1‑137 (MQ=255)
                                                                                                          cTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCACCCGTCCGGATcc                                  >  2:384232/1‑140 (MQ=255)
                                                                                                                    ttttACAAGCTTTTTATAATCGCTTTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTAGTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCa                        <  2:2946451/140‑1 (MQ=255)
                                                                                                                    ttttAAAAGGTTTTTATCATCGATTTGTAATAAAAAGCTCGTCAAAATTTTATCCTTTCGCACGAAAAAGATTTATTTGCCTTATATCCATTTTGTATTAAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCa                        <  1:2830786/140‑1 (MQ=255)
                                                                                                                    tttaACACGCGTTTTCTAATCGAATTGTCAAAAAAAGCTGGTGAAATTTTTATCCTTTCCCACAAAAATTTTTTAGTTGCCTGATATCCTTTTTGTATTCATCAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCa                        <  1:1806461/140‑1 (MQ=255)
                                                                                                                      ttccAAGCGTTTTATAATCGCATTGTCCTAAAAAGCTCGTTAAACTTTTATCCTTTAGCACTAAAAAGATTTACTTGCCTGATATCCTTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACg                      <  2:935578/137‑1 (MQ=255)
                                                                                                                       tACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACTAAAAAGAGTTAGTTGCCTGATAGCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGc                     <  2:3358966/140‑1 (MQ=255)
                                                                                                                            ggtttttCTCATCGCTTTGTCATAAAAAGCTGGTGAAATTTTTATCCTTTAGCACTAAAAAGTGTTACTGGCGTGATAGCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCa                <  2:522382/137‑1 (MQ=255)
                                                                                                                            ggtttttCTAATCGATTTGTCATAAAAAGCGCGTTAAATTTTTAGCATTTAGCACAAAAAAGTGTTCCTTGCCTTATAGCCCTTTTTTATTAATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCa                <  1:1317133/137‑1 (MQ=255)
                                                                                                                             gtttttCTCTTCTATTTTTCCTAAAACGCTCGTTAAAATTTTATCATTACGCACTAAAAAGTTTTAGTTGCGTTATATCCGTTTTTTATTCAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt               <  1:2146761/138‑1 (MQ=255)
                                                                                                                             gtttttCTCTTCGATTTGTCATAAAAAGCTCGTTAAAATTTTATCATTTAGCACCAAAAAGAGTTACTGGCTTCATATCCTTTTTGTTTTAAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt               <  1:1412433/138‑1 (MQ=255)
                                                                                                                             cattttCTCATCGAATTGGACTAAAAAGATCGTGAAAGTTTTATCATTTAGCACTAAAAAGAGTTACTTGCCTGTGACCCCTTGTGTTTGCATAAAAACGACTCGCGAACCGTCCGGATCCTCTATCGTCCCGCCTCCAt               <  2:2840064/138‑1 (MQ=255)
                                                                                                                                tttCTCATGGCATTGTCATAAAAAGCTCGTGAAAATTTTAGCCTTTCGCAAGAAAAAGAGTTACTGGCTTTATCACCCTTTTGTATGCAAAAAAACGACTCGCCAACCGTCAGGATCCTCTATCGTCCCGCCTCTATGGc            <  2:273940/140‑1 (MQ=255)
                                                                                                                                tttCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGc            <  2:1843052/140‑1 (MQ=255)
                                                                                                                                tgtctaATCGAATTGTCCTAAAAAGCTCGTCAAACTTTTATCCTTTAGAACTAAAAAGTGTTAGTGGCCTGATATCCCTTTTTTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGc            <  2:1162501/138‑1 (MQ=255)
                                                                                                                                  tctcATCGCATTGTCCTAAAAAGCCCGCGAAACTTTTATCCTTTAGCACCAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTc          <  2:2250607/140‑1 (MQ=255)
                                                                                                                                       tCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTa     >  2:261864/1‑140 (MQ=255)
                                                                                                                                         gCATTGTCCTAAAAAGCTCTTCAAACTTTTATCCTTTCGCACCAAAAAGAGTTACTGGCCTTATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACg   <  2:1367602/140‑1 (MQ=255)
                                                                                                                                          cATTGTCATAAACAGCTCGTCAAACTTTTATCGTGTAGCACGAAAAAGAGTTAGTTGCCTGATAGCCCTTTTTTTTTCAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACgg  <  2:1558982/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
AACCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGG  >  NZ_CP034943/3996712‑3996989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: