Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,996,891 G→T 8.8% intergenic (‑197/+9) purA ← / ← EO946_RS20530 adenylosuccinate synthase/VOC family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,996,8910GT 8.8% 540.9 / 3.7 249intergenic (‑197/+9)purA/EO946_RS20530adenylosuccinate synthase/VOC family protein
Reads supporting (aligned to +/- strand):  ref base G (132/62);  new base T (2/31);  total (134/115)
Fisher's exact test for biased strand distribution p-value = 7.33e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 249 total aligned reads displayed.

AGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTT  >  NZ_CP034943/3996753‑3997025
                                                                                                                                           |                                                                                                                                      
            gAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACt                                                                                                                           >  1:3066598/1‑140 (MQ=255)
            gAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACt                                                                                                                           >  1:440137/1‑140 (MQ=255)
                   aaTTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGa                                                                                                                    <  2:2189718/140‑1 (MQ=255)
                   aaTTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGa                                                                                                                    <  2:3044795/140‑1 (MQ=255)
                      tGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGatat                                                                                                                 <  1:2650181/140‑1 (MQ=255)
                           aTCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAATCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGACTGATATCCCtt                                                                                                            >  2:640940/1‑140 (MQ=255)
                           aTCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCtt                                                                                                            >  1:522382/1‑140 (MQ=255)
                              ctcaCATTAAATTAATGTATATCAATAATCGTTCTCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTCAAACTTTTATCCTTTACCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTg                                                                                                         <  2:2336932/137‑1 (MQ=255)
                               gAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGt                                                                                                        >  2:4002382/1‑140 (MQ=255)
                                  aattaacttaaTCTAAAACAATATTCGTTCGCGCCCAGGGGTTTAAAAAGCGTTTTCGCATCGCATTGTCCTAAAAACCACGTGAAACTTTTAAACTTTAGCACCAAAAAGAGTTACTTGCCTTAAACCACTTTTGTAta                                                                                                     <  2:4183734/139‑2 (MQ=255)
                                       aattaaTGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATaa                                                                                                >  1:397004/1‑140 (MQ=255)
                                       aattaaTGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATaa                                                                                                >  2:44387/1‑140 (MQ=255)
                                           aaTTTAAATCATTATTCTTTCGCTTCCATTGGTTTTACATTCGTTTTCTCTTCGCATTGTCATAAAAAGTTCTTTAAACTTTTATCCTTTAGCACGAAAAATTGTTACTGGCCTTATTTCCCTTTTGTATTCAAAAacac                                                                                            <  2:1399985/140‑1 (MQ=255)
                                             tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                                                          >  1:661670/1‑140 (MQ=255)
                                             tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                                                          >  1:4296521/1‑140 (MQ=255)
                                             tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                                                          >  1:2835868/1‑140 (MQ=255)
                                             tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                                                          >  2:2208879/1‑140 (MQ=255)
                                             tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                                                          >  1:3351272/1‑140 (MQ=255)
                                             tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGACTTACATGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                                                          >  1:1835957/1‑140 (MQ=255)
                                                 aaTCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCg                                                                                      >  1:2380779/1‑140 (MQ=255)
                                                 aaTCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCg                                                                                      >  1:1503193/1‑140 (MQ=255)
                                                      atatTCTTTCGTTTCAATTCGTTTTAAAAGCGTTTTATCATCGAATTTTAATAAAAAGCTCGTTAAACTTTTATCCTTTAGCACGAAAAAGAGTTAGTTGCCTGATATCCCTTTTTTATTCATAAAAACGACTCGCCAAc                                                                                 <  2:2572639/140‑1 (MQ=255)
                                                           cgttcgCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGa                                                                                                                    <  1:2540182/100‑1 (MQ=255)
                                                           cgttcgCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGa                                                                                                                    >  2:2540173/1‑100 (MQ=255)
                                                           cgttcgCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTcc                                                                            >  2:1302913/1‑140 (MQ=255)
                                                             ttcgGTTCCTTTCTTTTTACAAGCTTTTTCTCATCGCATTGTCCTAAAAAGCTCTTTAAAATTTTATCCTTTAGAACTAAAAAGAGTTACTTGCCTTATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCgg                                                                          <  2:3822123/140‑1 (MQ=255)
                                                              gcgCTTCCATTCGTTTTACAAGCTTTTTCTCATCGAATTGTCCTAAAAAGCTCCTGAAACTTTTATCCTTTAGCCCCAAAAAGAGTTACTTGCCTTATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGa                                                                         <  2:1204618/139‑1 (MQ=255)
                                                                 cTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCGTAAAAAGCTCGTGAAACTTTTATCCTGTAGCACTAACAAGAGTTACTTGCCTGAGATCGCGTTTGTATTCATAAAAACGACTCGCCCACCGTCCGGAGcc                                                                      >  1:2270804/1‑140 (MQ=255)
                                                                 cTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAGCAGTTCCTTGCCTGATATCCCTTTTGTATGAATAAAAACGACACGCCCACCGGCCGGagcg                                                                      >  1:2564255/1‑137 (MQ=255)
                                                                           ttttACAAGCTTTTTATAATCGCTTTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTAGTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCa                                                            <  2:2946451/140‑1 (MQ=255)
                                                                           tttaACACGCGTTTTCTAATCGAATTGTCAAAAAAAGCTGGTGAAATTTTTATCCTTTCCCACAAAAATTTTTTAGTTGCCTGATATCCTTTTTGTATTCATCAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCa                                                            <  1:1806461/140‑1 (MQ=255)
                                                                            tttACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCAc                                                           >  1:2908686/1‑140 (MQ=255)
                                                                            tttACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTGTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCAc                                                           >  2:2770522/1‑140 (MQ=255)
                                                                             ttACATGCTTTTTCTCATCGTTTTTTCATAAAAAGCTCGTTAAACTTTTATCGTTTAGGACGAAAAACTGTTACTTGACTGATATCCCTTTTGTTTTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACg                                                          <  2:3106920/140‑1 (MQ=255)
                                                                             ttACAAGTTTTTTCTCATCGCATTGTCATAAAAAGCTCTTGAAACTTTTATCCTTTAGCACCAAAAAGAGTTAGTTGCCTGATATCCCTTTTTTTTTAATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACg                                                          <  2:755666/140‑1 (MQ=255)
                                                                             ttACAAGGGTTTTCTAATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTTTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACg                                                          <  2:1953850/140‑1 (MQ=255)
                                                                              tACATGCTTTTTCTCATCGCATTCTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCTAGAAAAAGAGTAACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGc                                                         <  2:862036/140‑1 (MQ=255)
                                                                              tACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACTAAAAAGAGTTAGTTGCCTGATAGCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGc                                                         <  2:3358966/140‑1 (MQ=255)
                                                                              aacacGCTTTTTCTCATGGGTTTGTCAAAAAAAGCTCTTCAAACTTTTAGCATTTCGCCCCAAAAATAGTTACTGCCCTGATACCCCTTTTTTATAAAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGc                                                         <  1:1445382/139‑1 (MQ=255)
                                                                                   ggtttttCTCATCGCTTTGTCATAAAAAGCTGGTGAAATTTTTATCCTTTAGCACTAAAAAGTGTTACTGGCGTGATAGCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCa                                                    <  2:522382/137‑1 (MQ=255)
                                                                                   gctttttCACATCGATTGGTCCTAAAACGATCGTGAACCTTTTATCCTTTAGCACGAAAAAGAGTTAGTGGCCGTATATCCCTTTTGTTTTCTTAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCa                                                    <  2:4140528/137‑1 (MQ=255)
                                                                                    ctttttATCATCGAATTTTCATAAAAATCTCTTTAAATTTTTATCCTTTAGAACAAAAAAGTGTTACTTGCCTTATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                                                   <  2:3945617/138‑1 (MQ=255)
                                                                                    cattttCTCATCGAATTGGACTAAAAAGATCGTGAAAGTTTTATCATTTAGCACTAAAAAGAGTTACTTGCCTGTGACCCCTTGTGTTTGCATAAAAACGACTCGCGAACCGTCCGGATCCTCTATCGTCCCGCCTCCAt                                                   <  2:2840064/138‑1 (MQ=255)
                                                                                    cGTTTTCTCATCGCTTTTTCAAAAAACGCTGTTTAAATTTTTATCCTTTACCAACAAAAATATTTACTTGCCTGATATCCCTTTTGTATTAAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                                                   <  1:1322457/140‑1 (MQ=255)
                                                                                    cGTTTTCTCATCGAATTGTCCTAAAAAGCTCGTTAAACTTTTATCCTTTAGCACTAAAAAGAGTTACTTGCCTGATATCCGTTTTGTTTTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                                                   <  2:2021397/140‑1 (MQ=255)
                                                                                       tttCTCATGGCATTGTCATAAAAAGCTCGTGAAAATTTTAGCCTTTCGCAAGAAAAAGAGTTACTGGCTTTATCACCCTTTTGTATGCAAAAAAACGACTCGCCAACCGTCAGGATCCTCTATCGTCCCGCCTCTATGGc                                                <  2:273940/140‑1 (MQ=255)
                                                                                       tttCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGc                                                <  2:1843052/140‑1 (MQ=255)
                                                                                       tgtctaATCGAATTGTCCTAAAAAGCTCGTCAAACTTTTATCCTTTAGAACTAAAAAGTGTTAGTGGCCTGATATCCCTTTTTTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGc                                                <  2:1162501/138‑1 (MQ=255)
                                                                                       tgactcctcGCATTGTCCTAAAAAGCTGGTGAAACTTTTATCCTTTAGCACGAAAAACAGTTAGTTGCCTCATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGc                                                <  2:979792/137‑1 (MQ=255)
                                                                                          ctaaTCGCATTTTCCTAAAAAGCTCGTGAAATTTTTATCCTTTAGCACTAAAAATATTTACTTGCCTGATATCCCTTTTGTATTAATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTcc                                             <  2:1833062/137‑1 (MQ=255)
                                                                                              tctctttGTCATAAACAGATCGTTAAATTTTTATCCTTTAGCACTAAAAAGAGTTAGTTGCGTTATATCCGTTTTGTATTAATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTa                                         <  1:2196606/135‑1 (MQ=255)
                                                                                              tCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTa                                         >  2:261864/1‑140 (MQ=255)
                                                                                                 cATTGTCATAAACAGCTCGTCAAACTTTTATCGTGTAGCACGAAAAAGAGTTAGTTGCCTGATAGCCCTTTTTTTTTCAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACgg                                      <  2:1558982/140‑1 (MQ=255)
                                                                                                     gTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTtc                                  >  2:2995349/1‑140 (MQ=255)
                                                                                                           aaaaGCTCTTTAAACTTTTATCCTTTACCACGAAAAAGATTTAGTTGCCTTTTATCCTTTTTTTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGAt                            <  2:579652/140‑1 (MQ=255)
                                                                                                           aaaaGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGAt                            >  2:3860276/1‑140 (MQ=255)
                                                                                                           aaaaGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGAt                            >  2:2597452/1‑140 (MQ=255)
                                                                                                             acgcacGTCAAATTTTAATCCTTAAGAACCAAAAAGATTTCCTTGCCTTATTTCCTTTTTTTTTTAATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTc                          <  1:2708764/135‑1 (MQ=255)
                                                                                                             acgCTCGTGAAAGTTTTATCATTTACCACTAAAAAGAGTTAGTTGCGTGATAGCCCTTTTGTATACAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTc                          <  1:3832312/138‑1 (MQ=255)
                                                                                                             acgCTCGTGAAACTTTTATCCTTTAGCACCAAAAAGAGTTACTTGCCTTAAAGCCCTTTTGTATACATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTc                          <  1:844116/138‑1 (MQ=255)
                                                                                                             aaGCTCGTTAAAATTTATCCTTTTCGCACCAAATTCAGTTTCTTGACTTTTTTGCCTTTTTTTTACATAAAAACGACTCGCAAACCGTCCGGATCCTCTATCGTCACGCCTGCATGGCTCCAGTACGGGTTCTCAGATTc                          <  1:1276945/140‑1 (MQ=255)
                                                                                                             aaGCTCGTCAAAGTTTTATCCTTGACCACTAAAAAGAGTTACTTGCCTTATATCCCTTTTGTTTTCATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTc                          <  2:371084/140‑1 (MQ=255)
                                                                                                             aaGCTCGTCAAACTTTTATCCTTTACCACCAAAAAGAGTTACTTGCCTGATAGCCCTTTTGTATTCAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTc                          <  1:1367344/140‑1 (MQ=255)
                                                                                                               gCTGGTCAAAATTTTAACCTTTATCCCTAAAAAGAGTTATTTGATTGATTGCGATTTTTTCTTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCaa                        <  1:3109474/140‑1 (MQ=255)
                                                                                                                ctctTGAAACTTTTATCCTTTAGCAATAAAAATATTTAATTGCCTTATATCCGTTTTTTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                       <  2:35270/140‑1 (MQ=255)
                                                                                                                cTCGTGAAATTTTTATCCTTTAGAACGAAAAAGAGTTAGTTGCCTTATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                       <  2:694398/140‑1 (MQ=255)
                                                                                                                cTCGTGAAACTTTTATCCTTTAGCACGAAAAATAGTGACTTGCCTGAGATCCCTGTTGTAGTCATAAAATCGACTCGCCAACCGTCCGGATCCTCTCTCGGCACGCCTCCATGGATCGAGGACGGGGTCTCAGAttgacc                       >  2:206817/1‑136 (MQ=255)
                                                                                                                cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                       >  1:3264695/1‑140 (MQ=255)
                                                                                                                cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                       >  1:352908/1‑140 (MQ=255)
                                                                                                                cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                       >  1:2426747/1‑140 (MQ=255)
                                                                                                                cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                       >  1:2894904/1‑140 (MQ=255)
                                                                                                                cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                       >  2:1732491/1‑140 (MQ=255)
                                                                                                                cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                       >  2:4281995/1‑140 (MQ=255)
                                                                                                                cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                       >  1:1918485/1‑140 (MQ=255)
                                                                                                                cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                       >  2:949732/1‑140 (MQ=255)
                                                                                                                cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                       >  2:1913658/1‑140 (MQ=255)
                                                                                                                cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                       >  2:1930834/1‑140 (MQ=255)
                                                                                                                cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                       >  1:982863/1‑140 (MQ=255)
                                                                                                                cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                       >  1:3707862/1‑140 (MQ=255)
                                                                                                                cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                       >  2:2413144/1‑140 (MQ=255)
                                                                                                                cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                       >  1:4070834/1‑140 (MQ=255)
                                                                                                                cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                       >  2:267667/1‑140 (MQ=255)
                                                                                                                cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                       >  1:694398/1‑140 (MQ=255)
                                                                                                                cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCACTACGGGTTCTCAGATTCTAc                       >  2:2867959/1‑140 (MQ=255)
                                                                                                                cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGGATTCATAAAAACGACTCGCCAAGCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCCGATTCTAc                       >  1:2788264/1‑140 (MQ=255)
                                                                                                                 tCGTGAAACTTTTATCCTATAGCACGACACAGATTTCCTTCCCTTATAGCCATTTTTTTTTCATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAcc                      <  1:1011525/140‑1 (MQ=255)
                                                                                                                  cGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAccg                     >  2:4285882/1‑139 (MQ=255)
                                                                                                                    tGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACctct                   >  2:1274779/1‑140 (MQ=255)
                                                                                                                              aTCCTTTCCCACCAAAAAGCGTTACTTGCCTGATATCCCTTTTGTATTCATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCa         <  1:2086321/140‑1 (MQ=255)
                                                                                                                              aTCCTTTAGCACGAAAAATAGTTACTTGCCTTATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCa         <  2:1098117/140‑1 (MQ=255)
                                                                                                                                     tAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGtt   >  2:2627076/1‑140 (MQ=255)
                                                                                                                                     tAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGtt   >  1:778299/1‑140 (MQ=255)
                                                                                                                                     tAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGtt   >  2:2269285/1‑140 (MQ=255)
                                                                                                                                     tAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGtt   >  1:3232009/1‑140 (MQ=255)
                                                                                                                                     tAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGtt   >  2:1908542/1‑140 (MQ=255)
                                                                                                                                     tAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAAAAACTCGCCAACCGTCCGGATCCTCTATCGTCACGCATCCATGGCTCCAGTACGGGATCTCAGATTCAACACATTGATAGCCCATGTGtt   >  1:3617613/1‑140 (MQ=255)
                                                                                                                                     tAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTAGTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGtt   >  1:1134632/1‑140 (MQ=255)
                                                                                                                                      cgCACGAAAAAGCGGTCGTGGCCGGCTACGCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGttt  <  2:3604098/139‑1 (MQ=255)
                                                                                                                                      aGCCCGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGttt  <  2:3177154/140‑1 (MQ=255)
                                                                                                                                      aGCACGAAAAAGTGTTCCTGGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGttt  <  2:802358/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                      
AGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTT  >  NZ_CP034943/3996753‑3997025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: