Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 549,057 C→A 5.4% F23L (TTC→TTA EO946_RS02865 → PLP‑dependent aminotransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943549,0570CA 5.4% 700.4 / 3.3 253F23L (TTC→TTAEO946_RS02865PLP‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base C (122/107);  new base A (2/22);  total (124/129)
Fisher's exact test for biased strand distribution p-value = 1.70e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 254 total aligned reads displayed.

AACTGGAATTGGCCTTTTATAAACAGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCG  >  NZ_CP034943/548918‑549193
                                                                                                                                           |                                                                                                                                        
 aCTGGAATTGGCCTTTTATAAACAGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCa                                                                                                                                         <  2:901706/140‑1 (MQ=255)
    ggAATTGGCCTTTTATAAACAGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAg                                                                                                                                      >  1:3377600/1‑140 (MQ=255)
     gAATTGGCCTTTTATAAACAGCCAGTTTTCACAAAAAAATGCATACCATTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGa                                                                                                                                     >  1:558558/1‑140 (MQ=255)
     gAATTGGCCTTTTATAAACAGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGACCAACCCACATGGATATCACGCCTTTTTTGAATAGAAATCTGGCTATTCCGCGTGATCAGCAGCTTGATCGCTCTTTCAAAGa                                                                                                                                     >  2:3939006/1‑140 (MQ=255)
     gAATTGGCCTTTTATAAACAGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGa                                                                                                                                     >  2:2758934/1‑140 (MQ=255)
     gAATTGGCCTTTTATAAACAGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGa                                                                                                                                     >  1:2466190/1‑140 (MQ=255)
     gAATTGGCCTTTTATAAACAGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGa                                                                                                                                     >  1:1754472/1‑140 (MQ=255)
     gAATTGGCCTTTTATAAACAGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGa                                                                                                                                     >  2:1055418/1‑140 (MQ=255)
     gAATTGGCCTTTTATAAACAGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGa                                                                                                                                     >  1:4174875/1‑140 (MQ=255)
      aaTTGGCCTTTTATAAACAGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGaa                                                                                                                                    >  2:2046679/1‑140 (MQ=255)
      aaTTGGCCTTTTATAAACAGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGaa                                                                                                                                    >  2:238245/1‑140 (MQ=255)
      aaTTGGCCTTTTATAAACAGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGaa                                                                                                                                    >  2:2591586/1‑140 (MQ=255)
      aaTTGGCCTTTTATAAACAGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGaa                                                                                                                                    >  1:2270968/1‑140 (MQ=255)
      aaTTGGCCTTTTATAAACAGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATAAGCAGCTTTATCGCTATTTCAAAGaa                                                                                                                                    >  2:1676117/1‑140 (MQ=255)
      aaTTGGCCTTTTATAAACAGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTGTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGaa                                                                                                                                    >  2:3562551/1‑140 (MQ=255)
          gtccTTTTATAAACAGCCAGTTGTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACa                                                                                                                                <  2:3654970/138‑1 (MQ=255)
           gCCTTTTATAAACAGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACAt                                                                                                                               >  2:2635665/1‑140 (MQ=255)
            ccTTTTATAAACAGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATg                                                                                                                              <  1:1550709/140‑1 (MQ=255)
            ccTTTTATAAACAGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATg                                                                                                                              <  2:3905951/140‑1 (MQ=255)
                          ccAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACTCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTAt                                                                                                                >  2:2923830/1‑140 (MQ=255)
                          ccAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACa                                                                                                                                >  2:599199/1‑124 (MQ=255)
                          ccAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACa                                                                                                                                >  2:1548595/1‑124 (MQ=255)
                          ccAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACa                                                                                                                                <  1:1548595/124‑1 (MQ=255)
                          ccAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACa                                                                                                                                <  1:2193091/124‑1 (MQ=255)
                          ccAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTAt                                                                                                                >  1:1863295/1‑140 (MQ=255)
                          ccAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTAt                                                                                                                >  2:4276287/1‑140 (MQ=255)
                          ccAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTAt                                                                                                                >  1:1043034/1‑140 (MQ=255)
                          ccAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTAt                                                                                                                >  2:4216697/1‑140 (MQ=255)
                          ccAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCGTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTAt                                                                                                                >  1:1694310/1‑140 (MQ=255)
                                       aaaaaTGCATACCACTGAGGAGAACAACCCAAATCGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTAGTCAAAAAGGAATg                                                                                                   <  1:3780306/140‑1 (MQ=255)
                                        aaaaTGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGa                                                                                                  <  2:2316023/140‑1 (MQ=255)
                                            tGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCt                                                                                              <  1:1055418/140‑1 (MQ=255)
                                                      ttAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGACTGAAGCTCCCTTCCaca                                                                                    >  1:3753357/1‑138 (MQ=255)
                                                      ttAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCaaa                                                                                    >  2:2048623/1‑140 (MQ=255)
                                                      ttAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCaaa                                                                                    >  1:139257/1‑140 (MQ=255)
                                                       tAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCaaaa                                                                                   <  2:2104006/140‑1 (MQ=255)
                                                       tAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCaaaa                                                                                   <  1:2588071/140‑1 (MQ=255)
                                                       tAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCaaaa                                                                                   <  2:4287150/140‑1 (MQ=255)
                                                        aGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCTAAGAAAACATGCATCAGGGGCGGCTTCAAAAAGGAATGAAGCTCCCTTCCAagag                                                                                  >  1:4326163/1‑140 (MQ=255)
                                                        aGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAg                                                                                  >  1:3696812/1‑140 (MQ=255)
                                                         ggAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGa                                                                                 <  1:1233943/140‑1 (MQ=255)
                                                             aacaacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTCTTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTcc                                                                             >  2:1122896/1‑140 (MQ=255)
                                                             aacaacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGaaacc                                                                             >  2:3341795/1‑136 (MQ=255)
                                                             aacaacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTcc                                                                             >  2:3682399/1‑140 (MQ=255)
                                                             aacaacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTcc                                                                             >  2:1109046/1‑140 (MQ=255)
                                                               caacCCAAATGGATATCACGCCTTTTTTGAATAGAATTCTGGATATTCCGCTTTATCAGCAGCTTTCTCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGTAATGAAGCTCCCTTCCAAAAGACTCCTg                                                                           >  2:3873543/1‑140 (MQ=255)
                                                               caacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTCTTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTg                                                                           >  2:4061206/1‑140 (MQ=255)
                                                               caacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTg                                                                           >  1:3236698/1‑140 (MQ=255)
                                                               caacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTGCCAAAAGACTCCTg                                                                           >  2:3549352/1‑140 (MQ=255)
                                                                aacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTgg                                                                          >  1:4281284/1‑140 (MQ=255)
                                                                  cccAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGACGCTCCCTGCCAAAAGACTCCTGGCg                                                                        >  2:3984862/1‑140 (MQ=255)
                                                                     aaaTGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGt                                                                     <  1:1853012/140‑1 (MQ=255)
                                                                     aaaTGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGt                                                                     <  2:1863295/140‑1 (MQ=255)
                                                                            tatCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGACTGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCttt                                                              >  1:2042169/1‑140 (MQ=255)
                                                                            tatCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGTAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCttt                                                              >  1:311606/1‑140 (MQ=255)
                                                                            tatCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCt                                                                            >  1:806150/1‑126 (MQ=255)
                                                                            tatCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAAGGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCttt                                                              >  1:2214031/1‑140 (MQ=255)
                                                                                aCGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCAt                                                          >  1:3145674/1‑140 (MQ=255)
                                                                                aCGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCAt                                                          >  1:675358/1‑140 (MQ=255)
                                                                                 cGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCCTc                                                         <  2:1761959/140‑1 (MQ=255)
                                                                                 cGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATc                                                         <  2:1317705/140‑1 (MQ=255)
                                                                                 cGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATc                                                         <  1:4264196/140‑1 (MQ=255)
                                                                                    cTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAg                                                                      <  1:2521621/124‑1 (MQ=255)
                                                                                      ttttttATTTGAAATCTGTATATTCCGTTTTATAAGAAGTTTTATCGATATTTAAAATAAAACAGGCATCAGGGGCGTATAAAAAAAGGAATTACGCTCCCTTCAAAAAGACTCCTGGCGAGTCAGGTTTCCATCAGACa                                                    <  2:409866/140‑1 (MQ=255)
                                                                                      ttttttAATAGAAATCTGTATATTCCGCTTTATCAGCAGCTTTATCGATATTTCAAACAAAACATGCATCAGGGGCGTATTCAAAAAGTAATGAAGCTCCATTCCAAAAGACTCCTGGCGAGTCAGCTTTCCACCCGTCa                                                    <  2:138211/140‑1 (MQ=255)
                                                                                        ttttAATAGAAATATGTATTTTCCGCTTTATCTGATGCTTTATACCTATTTCAATGAAAACTTGCATCAGGGGTGTATTCAAAATGTAATGAAGATCCCTTCCAAATTACTCCTGGCGTGTCAGCTTTCCATCAGTCaaa                                                  <  2:2126057/140‑1 (MQ=255)
                                                                                        tttGAATAGAAATGTGTATTTTCCGCTTTATCAGCAGCTTTATGGATATTACAAACAAACCATCAATCAGGGGCTTATTCAAAATGGAATGATGCTCCCTTCAAAACCACTCCTGGCGAGTCAGCTTTCCATCAGTCaaa                                                  <  2:461776/140‑1 (MQ=255)
                                                                                        tttGAATAGAAATGTGTATATTCCGCTTTATCAGCAGCTTTATCTCTATTACAAAGAAAACATGCATCAGGGGGGTATTAAAAATGGATTGTAGCTCCCTCCAAAACCACTCCGGGCTTGTCAGGTGTCCATCCGCCaaa                                                  <  2:3307225/140‑1 (MQ=255)
                                                                                         tttaaTAGAAATCTGGTTTTTCGGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCAGCAGGGGCGTATTCAAAAAGGATTGAAGCTCCCTTCCAAAACACCCCGGGCGAGTCCGCTTTCCATCCGTCacac                                                 <  2:4318724/137‑1 (MQ=255)
                                                                                                     gtGGATATGCCGCTTTATCAGCAGCTTTATCGCTATTTCAAATAAAACATGATTCAGGGGGTTATTCAAAATTTAAGTAAGCTCCCTTCAAAAAGACTCCTGGGGTGTCAGCTTTCCATCAGACAAACGACTGTAGAACg                                     <  2:792967/139‑1 (MQ=255)
                                                                                                     ctttatatTCCTATTTATCTGGATCTTTTTCGCTATTTCAAACAAAACTTGCATGAGTGGCTTATTAAAAAATGATGTAAGCTCCCTTCAAAACCCCTCCTGGCGACTCAGCTTTCCATCAGTCAAACGAGTGTAGAACg                                     <  2:2422358/136‑1 (MQ=255)
                                                                                                     cTGTATATTCCGATTTATCAGACGCTTTATCGCTATTTCAAACAAAACATGCACCCGGGGCGTATACAAAAAGGAATGAAGCGCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCCGTCACACGACTGTAGAACg                                     <  1:2923830/140‑1 (MQ=255)
                                                                                                     cTGGATATTCCGCTTTCTCAGCAGATTTAGCGCTTTTTCAACTAAAACTGGCAACAGGGGCTTATTAAAAAAGTAATGACGCGCCCTTCCAAACGAGTCCGGGCGCGTCAGCTTTCCATCCGTCAAACGACTGTAGAACg                                     <  2:36121/140‑1 (MQ=255)
                                                                                                     cTGCATATTCCGCTTTAACAGCATCTTTATCCATATTTCAAACAAAACATGCATCCGGGGCTTATTAAAAATGAAATCAAGCGCCCTTCAAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTAAAACGACTGTAGAACg                                     <  1:3949882/140‑1 (MQ=255)
                                                                                                      tGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAATCATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGt                                    >  2:506364/1‑140 (MQ=255)
                                                                                                        gATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGc                                  >  1:3621404/1‑140 (MQ=255)
                                                                                                            ttCCGCTTTATCAGCAGCTTTATCTCTATTTCAAAGAAAACATGCAACAGGGGTTTATTAAAAACGTAATTACGCTCCCTTCAAAATTACACCTGGCTAGTCAGCTTACCATCAGTCAAACGACTGTAGAACGTGCCTAt                              <  2:17367/140‑1 (MQ=255)
                                                                                                             tacGCTTTATCAGCAGCTTTATATATATTTAAAATAAAACATGAATCAGGGGCTTATTCAAAAAGTAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATAAGTCAAACGACTGTAGAACGTGCCTATg                             >  2:1931058/3‑140 (MQ=255)
                                                                                                                    tATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCt                      >  2:964874/1‑140 (MQ=255)
                                                                                                                    tATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCt                      >  2:2499204/1‑140 (MQ=255)
                                                                                                                      tccccagcTTTATCTATTTTCAAAACAAAACATGAACCCGGGGCTTATAAAAAACGGAATGAAGCCCCCTACCAAAACCCGCGTGGCGAGTCAGCTTCCCCGCCGCCAAACGACTGTAGAACGTGCCTATGAACAGCTtg                    <  1:665514/136‑1 (MQ=255)
                                                                                                                      acagcagCTTTATCGCTATTAAAACGAAAACCTGCAACCGGGCGGTATGAAAAAAGTAATGACGCTCCCTTCCAAACGACCCCTGGCGCTGAAGCTTTCCAGCAGACAAACGACTGTAGAACGTGCCTATGAACAGCTtg                    <  1:4069652/139‑1 (MQ=255)
                                                                                                                      acaccagcTTTATCGCTTTTTAAAACAAAACAGGCATCCGGGGCGTATTCAAAAAGGAATGACCCTCCCTTCCAAAACACTCCTGGCGAGACAGCTTTCCAACAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTtg                    <  1:2139273/136‑1 (MQ=255)
                                                                                                                           agttttATCGATTTTTAAAATAAAACATGCATCCGGGGCGTATTAAAAAAGTAATGAAGCTCCCTTCCACAACACGCCTGGCTAGTACGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct               <  2:84258/137‑1 (MQ=255)
                                                                                                                           agttttATCGATATTAAAAATAAAACATGCATCAGGGGCTTATTAAAAAAGTAATCAAGCTCCCTACAAAACGACGCCGGGCGAGTCAGGTTTCCAACACACACACGACTGTAGAACGTGCCTATGAACAGCTTgctgct               <  1:1109046/137‑1 (MQ=255)
                                                                                                                           agcTTTATCGCTTTTAAAAAGAAAACCTGCATCCGGGGCTTATTAAAAAAGTATTGACGCGCCCTTCAAAACCCCTCCTGGCGAGTCAGTTTTCAATCACACACACGACTGTAGAACGTGCCTATGAACAGCTTgctgct               <  1:967793/140‑1 (MQ=255)
                                                                                                                           agcTTTATCGCTATTTCAAACAAAACCTGCATCCGGGGCGTATTCAAACCGGAATGACGCGCCCTTCCAAACCACGCCGGGCGAGTCAGCTTGCCAACCGTAAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct               <  2:3006149/140‑1 (MQ=255)
                                                                                                                           agcTTTATCGCTATTAAAAACAAAACATCCATCCGGGGCGTATTAAAAAAGTAATGAAGCTCCCTTCCAAAACACGCCTGGCGAGTCAGCTTTCCAACAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct               <  2:1377259/140‑1 (MQ=255)
                                                                                                                           agcTTTATCGATATTTCACACAAACCCTCCACCCGGGCCTTATCAAAAACGCAATGAAGCCCCCTACCAAACCACCCCGGCCGAGTCAGCTTACCAACCGCCACACGACTGTAGAACGTGCCTATGAACAGCTTgctgct               <  1:265376/140‑1 (MQ=255)
                                                                                                                           agcTTTATCGATATTTCAAATAAAAGATGATTCCGGGGCTTTTTCAAAAAGTAATGAAGCTCCCTTCCAAAACACTCCGGGCGTGTCAGCTTTCCAACAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct               <  2:3434018/140‑1 (MQ=255)
                                                                                                                           agcTTTATCGATATTACAAACAAAACAGCCATCAGGGGCTTATTCAAAAAGCAAGGAAGCTCCCTTCCAAAATACTCCTGGCTAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct               <  1:3004952/140‑1 (MQ=255)
                                                                                                                           agatttATCTCTTTTTAAAATAAAACTTGCATCAGGGGCTTATTCAAAAAGGAATGAAGCTCCCTTCCAAACGACCCCTGGCGAGTCAGCTTTCCATCAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTgctgct               <  2:3036741/137‑1 (MQ=255)
                                                                                                                             ttttATCGCTATTACAACTAAAACTTGCATCCGGGGATTATTAAAAACGTAAGCACGCTCCCTACCAAACGCCCCCTGGCGAGTCAGCTTTCCACCCGACACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa             <  1:2553736/139‑1 (MQ=255)
                                                                                                                             ttttATCGATATTTCAAACAAAACATGCATCTGGGGCGTATAAAAAAAGTAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTACCATCATTCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa             <  1:2066888/139‑1 (MQ=255)
                                                                                                                             ttttATCGATATTAAAAATAAAACATGCATCAGGGGCTTATTAAAAACGCAATGACGCTCCCTTCAAAACCACTCCGGGCGAGTCAGCTTTCCATCAGAAACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa             <  1:681576/139‑1 (MQ=255)
                                                                                                                             cTTTAGCGCTATTTCAAACAAAACATCCACCCGGGGCGTATAAAAAAAGCAATGAAGCTCCCTTCAAACACCCTCCGGGCGAGTCAGCTTACCAACAGACACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa             <  2:814406/140‑1 (MQ=255)
                                                                                                                                 aTCGCTTTTCCAAACAAAACCTGCTTAGGGGGTTTATTCAAAACTGAATCACGCTCCCTTCCAAACCAAGCGGGGCTATCAAGCTTTCCCACAGACACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGc         <  1:1498283/140‑1 (MQ=255)
                                                                                                                                  tCGATATTACAACTAAAACATGCATCAGGGGCTTATTCAAAAAGGAATGAAGCTCCCTGCCAAAAGACGCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCt        <  2:3696812/140‑1 (MQ=255)
                                                                                                                                   ctcTATTAAAAACAAAACATCCACCAGGGCCTTTATAAAAAAGTATTGAACCTCCCTTCAAAAACCCGCCGGGCGAGACAGCTTTCCACCAGACACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCta       <  1:3506190/138‑1 (MQ=255)
                                                                                                                                        tttCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAAGGTAGCTCCCTTCCAAAAGACTCCTGGCGAGTCCGCTTTCGATCAGTCAATCGAGTGTAGAACGTGCCTTTGAGCAGCGGGCTGCGGCGGGCtcttgcg  >  2:3059508/1‑134 (MQ=255)
                                                                                                                                           |                                                                                                                                        
AACTGGAATTGGCCTTTTATAAACAGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCG  >  NZ_CP034943/548918‑549193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: