Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 607,260 G→C 6.2% A35P (GCC→CCC)  EO946_RS03185 → collagen‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943607,2600GC 6.2% 727.6 / 4.4 260A35P (GCC→CCC) EO946_RS03185collagen‑like protein
Reads supporting (aligned to +/- strand):  ref base G (116/120);  new base C (15/2);  total (136/124)
Fisher's exact test for biased strand distribution p-value = 1.87e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 297 total aligned reads displayed.

ACCACAAGGACCACAAGGACCACAAGGACCACAAGGACTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGG  >  NZ_CP034943/607121‑607399
                                                                                                                                           |                                                                                                                                           
   acaaggaccacaaggaccacaaggaccacgaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGgaccagccggaccagccg                                                                                                                                          >  1:1594827/1‑140 (MQ=255)
   acaaggaccacaaggaccacaaggaccacaaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACTGGAGCCACGGGAGCTACTGGGGAAGCTGgaccagccggaccagccg                                                                                                                                          >  1:2293429/1‑140 (MQ=255)
   acaaggaccacaaggaccacaaggaccacaaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGgaccagccggaccagccg                                                                                                                                          >  1:3878538/1‑140 (MQ=255)
      aggaccacaaggaccacaaggaccacaaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAg                                                                                                                                       >  2:3204687/1‑140 (MQ=255)
      aggaccacaaggaccacaaggaccacaaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGCACCAGCCGGAg                                                                                                                                       >  2:3708369/1‑140 (MQ=255)
      aggaccacaaggaccacaaggaccacaaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACGGGGGAAGCAGGACCAGCCGGACCAGCCGGAg                                                                                                                                       >  2:1727768/1‑140 (MQ=255)
          ccacaaggaccacaaggaccacaaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGAc                                                                                                                                   >  2:2394017/1‑140 (MQ=255)
          ccacaaggaccacaaggaccacaaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGAc                                                                                                                                   >  2:3373785/1‑140 (MQ=255)
          ccacaaggaccacaaggaccacaaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGAc                                                                                                                                   >  2:3238381/1‑140 (MQ=255)
          ccacaaggaccacaaggaccacaaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGAc                                                                                                                                   >  1:3984532/1‑140 (MQ=255)
          ccacaaggaccacaaggaccacaaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACCGGAGCTACGGGGGAAGCTGGACCAGCCGGACCCCCCGGAGCGAc                                                                                                                                   >  2:2760835/1‑140 (MQ=255)
          ccacaaggaccacaaggaccacaaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGCCCCACCGGAGCCACCGGAGCTACTGGGGAAGCTGGCCCAGCCCGACCACCCGCAGcccc                                                                                                                                   >  1:3418600/1‑137 (MQ=21)
           cacaaggaccacaaggaccacaaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACg                                                                                                                                  >  1:3855723/1‑140 (MQ=255)
            acaaggaccacaaggaccacaaggacTGCCGGGAGTCATGGGAGCCACCGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACgg                                                                                                                                 >  1:785709/1‑140 (MQ=255)
             caaggaccacaaggaccacaaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCGACCGCGGCTACAGGGCACGCGGGGCCCGCCGGAGCCCCCGGGGCGagcgg                                                                                                                                >  2:1005816/1‑136 (MQ=21)
                   ccacaaggaccacaaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCAc                                                                                                                          >  1:2334957/1‑140 (MQ=255)
                     acaaggaccacaaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACgg                                                                                                                        >  1:3074665/1‑140 (MQ=255)
                     acaaggaccacaaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACgg                                                                                                                        >  1:197710/1‑140 (MQ=255)
                     acaaggaccacaaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACgg                                                                                                                        >  2:1856311/1‑140 (MQ=255)
                      caaggaccacaaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACggg                                                                                                                       <  1:2391349/140‑1 (MQ=255)
                       aaggaccacaaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGa                                                                                                                      <  2:2878668/140‑1 (MQ=255)
                        aggaccacaaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGaccagccggaccagccgg                                                                                                                                         >  2:714140/1‑120 (MQ=255)
                        aggaccacaaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACAAGCCGGACCACCCGGAGCGACGGGAGACACGGGAg                                                                                                                     >  1:641079/1‑140 (MQ=255)
                           accacaaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACGAGCCGGAGCGACGGGAGCCACGGGAGCtc                                                                                                                  >  1:2237057/1‑139 (MQ=255)
                           accacaaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTa                                                                                                                  >  1:3103162/1‑140 (MQ=255)
                            ccacaaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTCCTGGGGAACCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTAc                                                                                                                 >  1:2107232/1‑140 (MQ=255)
                              acaaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGcacccg                                                                                                               >  2:3089112/1‑135 (MQ=255)
                               caaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCTGGGAAGCCGGACCAGCCGGGGCGACAGGAGGCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCgg                                                                                                              <  2:163015/140‑1 (MQ=255)
                               caaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCgg                                                                                                              <  2:3422913/140‑1 (MQ=255)
                                    ccggCCGGGAGTCATGGGAGCCACAGGAGCGACCGGGGCAGCCGGACCAGCCGGGGCGACCGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAg                                                                                                         <  2:3792126/137‑1 (MQ=255)
                                    acTGCCGGGAGTCATGGGAGCCACGGGAGCAACCGCGGAAGCCGGACAATCCTTGGCGACATGAGACAAGGGAGATACTGGGTAAGCTGGACCATCATGAGCAGCCGGAGCGACGGGCGCCACGGGAGCTACCGGGTAAg                                                                                                         <  2:787068/140‑1 (MQ=255)
                                    acTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAg                                                                                                         <  1:1941493/140‑1 (MQ=255)
                                    acTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAg                                                                                                         <  1:4098268/140‑1 (MQ=255)
                                    acTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCAGGACCAGCCGGAGCGACGGGCGCCACGGGAGCTACCGGGGAAg                                                                                                         >  2:2473916/1‑140 (MQ=255)
                                     cTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGc                                                                                                        >  1:3537844/1‑140 (MQ=255)
                                     cTGCCGCGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGACGc                                                                                                        >  1:724512/1‑140 (MQ=255)
                                       gCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGCCAGGAGACACCGGAGCGACACGGGCAGCTGGACCCGCCGGAGCACGCGGCGCGACGGGTGCCACGGGAGCTCCCGGGGACGcgg                                                                                                      >  2:36486/1‑138 (MQ=21)
                                         cGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGa                                                                                                    <  2:3300197/140‑1 (MQ=255)
                                             aGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGGGCTACGGGGGAACCGGGGCCAGCCGGACCAGCCGGAGCGACGGGCGCCACCGGAGCAGCCGGAGCAGCTGGagggg                                                                                                >  2:412986/1‑136 (MQ=18)
                                                cATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCg                                                                                             >  2:2410716/1‑140 (MQ=255)
                                                cATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACGGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCg                                                                                             >  2:1355200/1‑140 (MQ=255)
                                                  tGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCAc                                                                                                                          <  1:2666909/109‑1 (MQ=255)
                                                  tGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGa                                                                                           >  1:2960690/1‑140 (MQ=255)
                                                  tGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGa                                                                                           <  1:500569/140‑1 (MQ=255)
                                                  tGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCGGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCGGGACCAGCCGGa                                                                                           >  2:1482350/1‑140 (MQ=255)
                                                         cacaGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACgg                                                                                    >  1:4204453/1‑140 (MQ=255)
                                                          acaGGAGCTCCCGGCGAAGCCGGACCACCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACggg                                                                                   <  2:1325295/140‑1 (MQ=255)
                                                          acaGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACggg                                                                                   <  2:2265965/140‑1 (MQ=255)
                                                          acaGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACggg                                                                                   <  1:1372622/140‑1 (MQ=255)
                                                          acaGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACggg                                                                                   <  1:1323607/140‑1 (MQ=255)
                                                          acaGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACGGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACggg                                                                                   <  2:4240615/140‑1 (MQ=255)
                                                            aGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGACCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGCGCGACGGGAg                                                                                 <  2:1576478/140‑1 (MQ=255)
                                                            aGGAGCTACCGGGGAAGCCGGACCAGCCGGCGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAg                                                                                 <  2:1408716/140‑1 (MQ=255)
                                                                gCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGAc                                                                             <  2:2047372/140‑1 (MQ=255)
                                                                gCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGAc                                                                             <  2:3949122/140‑1 (MQ=255)
                                                                gCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGAc                                                                             <  1:2018235/140‑1 (MQ=255)
                                                                gCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGAc                                                                             <  1:3562048/140‑1 (MQ=255)
                                                                  tACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGTGCGACAg                                                                           <  2:2801171/140‑1 (MQ=255)
                                                                   aCCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAgg                                                                          >  1:80388/1‑140 (MQ=255)
                                                                   aCCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAgg                                                                          >  1:3638474/1‑140 (MQ=255)
                                                                   aCCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAgg                                                                          <  1:4151971/140‑1 (MQ=255)
                                                                   aCCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAgg                                                                          >  1:3217719/1‑140 (MQ=255)
                                                                   aCCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCGACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAgg                                                                          >  1:4285063/1‑140 (MQ=255)
                                                                                 aCCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGACGCTGGACCAGCCTGACCAGCCGGAGCGACGGGAGCCACCGGAGCTACCGGGGACGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGCag                                                            >  2:3152286/1‑140 (MQ=18)
                                                                                     gCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAagccgg                                                        <  2:1264166/140‑1 (MQ=255)
                                                                                     gCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAagccgg                                                        <  1:4315118/140‑1 (MQ=255)
                                                                                      ccGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAagccgga                                                       <  1:2503012/140‑1 (MQ=255)
                                                                                      ccGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAagccgga                                                       >  1:3717084/1‑140 (MQ=255)
                                                                                          ggCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGACGCAGGACCACCCGGAGCGACGGGAGCGACAGGAGCAACCGGCCCaaccggaccac                                                   >  2:2702648/1‑139 (MQ=18)
                                                                                               cAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAagccggaccagccgga                                              <  1:2799907/140‑1 (MQ=255)
                                                                                                    gCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAAc                                         <  2:2324710/140‑1 (MQ=255)
                                                                                                    gCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAAc                                         <  2:774385/140‑1 (MQ=255)
                                                                                                       aCGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCgg                                      <  2:3074665/140‑1 (MQ=255)
                                                                                                       aCGGGAGCTACGGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCgg                                      <  2:197710/140‑1 (MQ=255)
                                                                                                            aGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGa                                 >  1:3330438/1‑140 (MQ=255)
                                                                                                             gCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGCCCAACCGGAGCGAc                                >  2:3269090/1‑140 (MQ=255)
                                                                                                              cttcTGGGGAAGCTGGACCAGCCGGACCAGCCGGCGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGCCGGGAGCGCCAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCAACCGGAGCGCCg                               >  1:2844349/4‑140 (MQ=18)
                                                                                                              cTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGACCAACCGGAGCGACg                               >  1:2380468/1‑140 (MQ=255)
                                                                                                              cTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACg                               <  1:1143190/140‑1 (MQ=255)
                                                                                                              cTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACg                               <  2:4204453/140‑1 (MQ=255)
                                                                                                              cTACTGGGGAAGCTGGACCAGCCGCACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACg                               <  2:2159571/140‑1 (MQ=255)
                                                                                                                acgggggAAGCTGGACCAGCCGGACCTCCGGGAGCGACGGGAGCCACGGGAGCTACCGGTGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACagg                             <  2:2815283/137‑1 (MQ=17)
                                                                                                                aCTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACggg                             <  1:2728139/140‑1 (MQ=255)
                                                                                                                   ggggAAGCTGGACCAGCCGGACCCGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGc                          <  2:3597664/140‑1 (MQ=255)
                                                                                                                   ggggAAGCGGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGc                          <  2:386051/140‑1 (MQ=255)
                                                                                                                      gAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCAc                       <  2:2517502/140‑1 (MQ=255)
                                                                                                                         gCTGGACCAGCCGGACCTGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACggg                    <  1:1727768/140‑1 (MQ=255)
                                                                                                                         gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGATCGACGGGAGCGACAGGAGCTACCGGGGAAGGCGGACCCGCCGGACCCAGCGGAGCGACGGGAGCGACcgg                    >  2:2652994/1‑140 (MQ=18)
                                                                                                                         gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACggg                    >  1:3753623/1‑140 (MQ=255)
                                                                                                                         gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACggg                    >  1:1850224/1‑140 (MQ=255)
                                                                                                                         gCTGGACCACCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACggg                    <  2:417404/140‑1 (MQ=255)
                                                                                                                          cTGGACCAGCCGGACCTCACGGAGCGACGGGAGCCACTGGTGAAACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGa                   <  1:579148/140‑1 (MQ=18)
                                                                                                                          cTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACCGGAGCTACCGGAGCTACCGGGGAAGCCGGAGCAGCAGGACCAACCGGAGCGACGGGACCAACCGGa                   >  1:756862/1‑140 (MQ=18)
                                                                                                                           tGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAgg                                                                          <  2:1933555/84‑1 (MQ=255)
                                                                                                                              accagccggaccagccggAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTa               <  1:3464161/140‑1 (MQ=255)
                                                                                                                                   ccggaccagccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCAACCGGAGCGACGGGAGCCACGGGCGCTCCCggg          >  1:337122/1‑140 (MQ=255)
                                                                                                                                   ccggaccagccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCggg          <  2:4328963/140‑1 (MQ=255)
                                                                                                                                   ccggaccagccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCggg          <  1:14933/140‑1 (MQ=255)
                                                                                                                                       accagccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGTGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACg                               >  1:3250822/1‑115 (MQ=255)
                                                                                                                                           gccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccgg  <  2:3537844/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
ACCACAAGGACCACAAGGACCACAAGGACCACAAGGACTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGG  >  NZ_CP034943/607121‑607399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: