Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 607,359 A→C 15.6% T68P (ACC→CCC)  EO946_RS03185 → collagen‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943607,3590AC15.6% 548.8 / 20.0 296T68P (ACC→CCC) EO946_RS03185collagen‑like protein
Reads supporting (aligned to +/- strand):  ref base A (96/134);  new base C (4/53);  total (102/194)
Fisher's exact test for biased strand distribution p-value = 1.33e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 299 total aligned reads displayed.

AGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGG  >  NZ_CP034943/607220‑607498
                                                                                                                                           |                                                                                                                                           
aGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCaa                                                                                                                                             <  2:3093797/140‑1 (MQ=255)
 gCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAAc                                                                                                                                            <  2:2324710/140‑1 (MQ=255)
  ccACGGGAGCTACGGGGCCAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAAcc                                                                                                                                           <  2:785709/140‑1 (MQ=255)
     cGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCCGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGa                                                                                                                                        <  2:1594827/140‑1 (MQ=255)
      gggAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGACCCCCCGGAg                                                                                                                                       >  1:3471385/1‑140 (MQ=255)
          gCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCGACGGGAGCTACGGGGGAAGCTGGACCCGCGGGAGCGACGGGAGCGCGAGGGGCAGCCGGGGAAGCCGGAGCAGGCGGAGCGACGGGAGCggc                                                                                                                                   >  2:750524/1‑138 (MQ=14)
           cttcTGGGGAAGCTGGACCAGCCGGACCAGCCGGCGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGCCGGGAGCGCCAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCAACCGGAGCGCCg                                                                                                                                  >  1:2844349/4‑140 (MQ=18)
           cTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAgcggcg                                                                                                                                  <  2:1993124/140‑1 (MQ=255)
           cTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACg                                                                                                                                  <  2:4204453/140‑1 (MQ=255)
           cTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACg                                                                                                                                  <  1:1143190/140‑1 (MQ=255)
           cTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGCGAAGCCGGACCACCCGCACCAACCGGAGCGACg                                                                                                                                  >  1:2763912/1‑140 (MQ=255)
             aCTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACggg                                                                                                                                <  1:228333/140‑1 (MQ=255)
             aCTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACggg                                                                                                                                <  1:3667911/140‑1 (MQ=255)
                   gcaGCTGGCCCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCGGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCAc                                                                                                                          <  2:2574654/138‑1 (MQ=255)
                   gAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGCAGCAGGACCAGCCGGAGCCCCCGGAGCGACAGGAGCAACCGCTGAACCCGGTCCACCCGGACCCACCGGAGCGACAGGGGCGAc                                                                                                                          >  2:1810070/1‑140 (MQ=14)
                   gAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCAc                                                                                                                          <  2:2517502/140‑1 (MQ=255)
                      gCTGGACCAGCCGGACCTGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACggg                                                                                                                       <  1:1727768/140‑1 (MQ=255)
                      gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACggg                                                                                                                       >  1:791840/1‑140 (MQ=255)
                      gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACggg                                                                                                                       <  1:4138590/140‑1 (MQ=255)
                      gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACggg                                                                                                                       >  1:3296916/1‑140 (MQ=255)
                      gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACggg                                                                                                                       <  1:3204687/140‑1 (MQ=255)
                      gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACggg                                                                                                                       <  2:2786015/140‑1 (MQ=255)
                      gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACggg                                                                                                                       <  2:417598/140‑1 (MQ=255)
                      gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACcgg                                                                                                                       >  2:3183659/1‑140 (MQ=255)
                      gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCGACCGGCGCAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACggg                                                                                                                       >  2:3345344/1‑140 (MQ=255)
                      gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACCGGAGCAACCGGCGCAGCTGGACCAGCCGGAGCGACCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCACCCGGCGCACCCGGAGCGCCGGGGGCGCCggg                                                                                                                       >  1:2701471/1‑140 (MQ=17)
                       cTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCTACCGGAGCTACCGGGGcggacgca                                                                                                                      >  1:756751/1‑133 (MQ=17)
                           ccccgccggaccagccggaGCGCCGGGAGCCACGGGAGCGACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAACCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTa                                                                                                                  <  1:4082413/136‑1 (MQ=255)
                           accagccggaccagccggAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCCCGGGAGCTa                                                                                                                  >  2:1711859/1‑140 (MQ=255)
                           accagccggaccagccggAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTa                                                                                                                  <  1:4313094/140‑1 (MQ=255)
                           accagccggaccagccggAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAACCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCtc                                                                                                                  >  2:3501322/1‑139 (MQ=255)
                                ccggaccagccggaGTCACGGGAGCCACCGGAGCTACCGGGGAAGCGGGACCAGCCGGCGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCggg                                                                                                             <  2:3875170/140‑1 (MQ=17)
                                ccggaccagccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCggg                                                                                                             <  2:4328963/140‑1 (MQ=255)
                                ccggaccagccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCggg                                                                                                             <  1:14933/140‑1 (MQ=255)
                                ccggaccagccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCggg                                                                                                             >  1:3343916/1‑140 (MQ=255)
                                    accagccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGTGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACg                                                                                                                                  <  2:3250822/115‑1 (MQ=255)
                                         ccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCTGCCGGACCAACCGGAGCGACGGGAGCCACGGGGGCTACAGGGGAcgccgga                                                                                                    >  1:4165348/1‑140 (MQ=255)
                                         ccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccgga                                                                                                    <  1:3715403/140‑1 (MQ=255)
                                         ccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccgga                                                                                                    <  1:4249747/140‑1 (MQ=255)
                                             agtcaCGGGAGCCACCGGAGCTCCCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccag                                                                                                <  2:1257279/136‑1 (MQ=255)
                                             aGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccag                                                                                                <  2:2390845/140‑1 (MQ=255)
                                             aGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccag                                                                                                <  2:3638474/140‑1 (MQ=255)
                                                gACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTCCCGGGGAcgccggaccagccg                                                                                             >  1:1662491/1‑140 (MQ=255)
                                                  cGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccagccgga                                                                                           <  1:1876851/140‑1 (MQ=255)
                                                   gggAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccagccggac                                                                                          <  2:3753623/140‑1 (MQ=255)
                                                    ggAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccagccggacc                                                                                         <  1:2894179/140‑1 (MQ=255)
                                                                gCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGCGCGACCGGGGAAGCCGGACCACCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgac                                                                             <  1:3259511/140‑1 (MQ=255)
                                                                cctcccGGGGACCCAGGACCAGCCGGAGCGACGGGAGCCACAGGAGCTACCGGTGCAGCCGGACCAGCCGGACAAACGGGAGGGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggcgcgac                                                                             <  2:2421886/136‑1 (MQ=17)
                                                                 cTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgaca                                                                            <  2:4115977/140‑1 (MQ=255)
                                                                 cTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGAGAAGCCGGACCAGCCGGACCAACCggagcgaca                                                                            <  2:3562553/140‑1 (MQ=255)
                                                                   cccGGGGCACCCGGACCACCCGGAGCCACCGGAGCCACAGGACCTACCGGCCAAGCCGGACCAGCCGGACGACCCGGAGCCACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacagg                                                                          <  2:1133569/139‑1 (MQ=14)
                                                                        ggAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcga                                                                     <  2:475835/140‑1 (MQ=255)
                                                                            gCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGagcgacaggagcgacagg                                                                 >  1:1390647/1‑140 (MQ=255)
                                                                              tGGACCAGCCGGAGCGCCGGGAGCGACTGGAGCTACGGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGcgacaggagcgacaggag                                                               <  2:1737145/140‑1 (MQ=255)
                                                                              tGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGcgacaggagcgacaggag                                                               >  1:2049543/1‑140 (MQ=255)
                                                                              tGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGcgacaggagcgacaggag                                                               <  1:182594/140‑1 (MQ=255)
                                                                              cggACCAGCCGGAGCGCCGGGAGCGAGAGGAGCTACCGGGGCAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGcgacaggagcgacaggag                                                               <  2:2237057/139‑1 (MQ=255)
                                                                               ggACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACCGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCgacaggagcgacaggagc                                                              >  1:3542350/1‑140 (MQ=255)
                                                                                 cccAGCCGGAGCCCCGGGAGCGACAGGAGCCACGGGCGATCCCGGACAAGCCGGACCACCCGGAGCGACGGGAGCCACCGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTa                                                            <  2:2842568/139‑1 (MQ=14)
                                                                                 accaccCGGACCCACCGGACCCAAAGGACCTACAGGCCAACCCGGACCACCAGGACCAACCGGACCGACGGGAGACACGGGAGCTACCGGCGAACCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTa                                                            <  2:3896208/140‑1 (MQ=9)
                                                                                 aCCAGCCGGAGCGACGGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTa                                                            <  1:1564849/140‑1 (MQ=255)
                                                                                 aCCAGCCGGAGCGACGGGAGCCACAGGAGCTACAGGGGAACCCGGACCAGCCGGACCAGCCGGAGCGACGGGAGACACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTa                                                            <  2:3438952/140‑1 (MQ=14)
                                                                                     gCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGCGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCGACAGGAGCTCCCgg                                                        >  1:2529166/1‑140 (MQ=255)
                                                                                      ccGGAGCGCCGGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCggg                                                       <  1:2394017/140‑1 (MQ=255)
                                                                                      ccGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCggg                                                       <  1:3014898/140‑1 (MQ=255)
                                                                                          cgCGACGGGAGGGACCGGAGCTCCGTGGGACGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCGGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAg                                                   <  1:72821/139‑1 (MQ=14)
                                                                                          accGACGGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAg                                                   <  2:2747965/138‑1 (MQ=255)
                                                                                          aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCGACGGGAGCTACCGGGGACGCCGGACCAGCCGCAGCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGacg                                                   >  2:1662146/1‑138 (MQ=14)
                                                                                          aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAg                                                   >  2:550715/1‑140 (MQ=255)
                                                                                          aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAg                                                   <  1:1009954/140‑1 (MQ=255)
                                                                                          aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAg                                                   >  1:3811241/1‑140 (MQ=255)
                                                                                          aGCGACGGGAGCGAAAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGCCCACCCGGAGCGACCGGAGCGCCGGGCGCTACGGGGGacg                                                   >  1:2496293/1‑138 (MQ=17)
                                                                                               ctggAGCGACCGGAGCTACCGCGGACGCCGGACAAGCCGGACCAACCGGAGCGACGGGAGCCACGGGCGCTACCGGGGAACCCGTACCAGCCGGCCCAACCCGAGCGACAGGAGCGACAGGACCTACCGGGGAAGCCgca                                              <  2:370523/138‑3 (MQ=17)
                                                                                                gggAGCCACGGGAGCTCACGGTGATGCCGGACCAGCCGGACCACCCGGAGCGCCGGGAGCCACGGGAGCTACCGGGGAAGCCGGCCCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                             <  2:2958459/140‑1 (MQ=14)
                                                                                                    gCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACg                                                                                                                                  >  1:3389928/1‑51 (MQ=255)
                                                                                                    gCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCa                                                                                                                           <  1:1316149/58‑1 (MQ=255)
                                                                                                    gCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCa                                                                                                                           >  2:1910178/1‑58 (MQ=255)
                                                                                                       aCAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACTGGAGCGACTGGAGCTACCTGGGAAGACGGACCATCCGGACCAACAGGAGCGACAGGAGCTCCAGGTGCTCGCGGGGAAGCAGGACGAGCCgg                                      >  2:2653751/1‑140 (MQ=11)
                                                                                                       aCAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                      >  2:3306157/1‑140 (MQ=255)
                                                                                                       aCAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCAGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCAGGACCAGCCgg                                      >  2:3989422/1‑140 (MQ=255)
                                                                                                       aCAGGAGCGACCGGGGAAGCCGGACCAGCCGCACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                      <  2:3823291/140‑1 (MQ=255)
                                                                                                         aGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggag                                    <  1:3955490/140‑1 (MQ=255)
                                                                                                             gcgaCCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACTGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGTGGAAGCCGGACCAGCCggagcgac                                <  1:926360/137‑1 (MQ=255)
                                                                                                              cTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgaca                               <  1:3698890/140‑1 (MQ=255)
                                                                                                                aCCGGGGAAGCCGGACCAGCCGGACCGACCGGAGCGACCGGAGCAACGGGAGCAACCGGGGACGCCGGACCAGCGGGAGCAACCGGGGCGACCGGAGAGACCGGAGCAACCGGGGCAGCCGGAGCTGCCggggcgaccgg                             >  2:833145/1‑140 (MQ=14)
                                                                                                                      gAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgac                       >  1:1037348/1‑140 (MQ=255)
                                                                                                                        agccggaccagccggaccAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacag                     >  2:956297/1‑140 (MQ=255)
                                                                                                                        agccggaccagccggaccAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacag                     >  2:1437280/1‑140 (MQ=255)
                                                                                                                         gccggaccagccggagcaACCGGAGCGACGGGAGCCACGGGCGCACCCGGCGAGGCCGGAGCAACAGGAGCAACGGGAGCCCCAGGACCGACAGGAGCTCCCGGAGACCCCGGCGCCGCCggacagacaggagcgcccgc                    >  2:204340/1‑135 (MQ=12)
                                                                                                                         gccggaccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacagg                    >  2:4294869/1‑140 (MQ=255)
                                                                                                                         gccggaccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacagg                    >  2:959784/1‑140 (MQ=255)
                                                                                                                          ccggaccagccggaccaCCCGGAGCGTCGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacagga                   <  2:3105187/140‑1 (MQ=255)
                                                                                                                              accagccggaccaCCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcga               <  1:2332384/140‑1 (MQ=255)
                                                                                                                              accagcaggaccaACCGGAGCGACGGGAGCCAACGGAGCTACCGGGGCAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcga               <  2:1367865/140‑1 (MQ=14)
                                                                                                                                  gccggaccaCCCGGACCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAACCCGGACCAGCCGGagcgacaggagcgacaggagcgacagg           <  2:3060875/140‑1 (MQ=255)
                                                                                                                                  gccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGagcgacaggagcgacaggagcgacagg           <  2:4159604/140‑1 (MQ=255)
                                                                                                                                  ccaggaccaCCCGGAGCCCCCGGACCCCCCGGAGCACCCGGCGCACCCGGACCACCCGGACGCCCGGGAGGGAGGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGagcgacaggagcgacaggagcgacagg           <  2:1462035/137‑1 (MQ=2)
                                                                                                                                   ccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCAACCGGAGCGACAGGGGCGACAGGAGCTCCCGGGGACGCCGGACCAGCCGGagcgacaggagcgacaggagcgacaggc          >  1:998411/1‑139 (MQ=255)
                                                                                                                                       accaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTa      >  1:607514/1‑140 (MQ=255)
                                                                                                                                           aCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCTACAGGAGAGTCAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGCCCGGGGCGCCAGGAGCGACGGGCGCTCCCgg  >  1:4029207/1‑140 (MQ=14)
                                                                                                                                           |                                                                                                                                           
AGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGG  >  NZ_CP034943/607220‑607498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: