Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 704,348 Δ1 bp 100% intergenic (+170/‑14) EO946_RS03650 → / → EO946_RS03655 CamS family sex pheromone protein/phosphotransferase enzyme family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943704,3480A.99.1% 573.0 / ‑2.1 117intergenic (+170/‑14)EO946_RS03650/EO946_RS03655CamS family sex pheromone protein/phosphotransferase enzyme family protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base . (46/70);  total (46/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 144 total aligned reads displayed.

GCACAAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTATGTATATGA  >  NZ_CP034943/704209‑704483
                                                                                                                                           |                                                                                                                                       
gCACAAGGGTTTTTTTATGTTACAATTAACAGGAATAGGCAAAAGATGTATTTTTGCTTCTGCCGAGACACAACTCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAACACAATCACTATCAAATCAATAGCaaaga                                                                                                                                         >  2:446673/1‑140 (MQ=255)
gCACAAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACACATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAAAAATCAATATAAAATCAATAGCaaaga                                                                                                                                         >  1:176417/1‑140 (MQ=255)
gCACAAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAACACAATCAATATCAAATCAATAGCcaaga                                                                                                                                         >  1:1396126/1‑140 (MQ=255)
gCACAAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATCTCAAATCAATCGCcaaga                                                                                                                                         >  1:2171766/1‑140 (MQ=255)
gCACAAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTGTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaaga                                                                                                                                         >  2:523147/1‑140 (MQ=255)
 cacaAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaa                                                                                                                                        >  1:1007700/1‑140 (MQ=255)
  agaaGTGTTTTTTTATGTTACCATTAACAGGAATAGGAAAAATATCTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaag                                                                                                                                       <  2:1413445/138‑2 (MQ=255)
  acaATGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaag                                                                                                                                       <  2:2424572/140‑2 (MQ=255)
  acaAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaag                                                                                                                                       <  1:1937849/140‑2 (MQ=255)
  acaAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaag                                                                                                                                       <  2:931718/140‑2 (MQ=255)
  acaAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaag                                                                                                                                       <  1:2012547/140‑2 (MQ=255)
  acaAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaag                                                                                                                                       <  1:2050847/140‑2 (MQ=255)
  acaAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaag                                                                                                                                       <  1:2131100/140‑2 (MQ=255)
  acaAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaag                                                                                                                                       <  2:1502239/140‑2 (MQ=255)
  acaAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaag                                                                                                                                       <  1:2224724/140‑2 (MQ=255)
  acaAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaag                                                                                                                                       <  2:1001917/140‑2 (MQ=255)
  acaAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaag                                                                                                                                       <  1:849258/140‑2 (MQ=255)
  acaAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaag                                                                                                                                       <  1:499340/140‑2 (MQ=255)
  acaAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaag                                                                                                                                       <  1:67004/140‑2 (MQ=255)
  acaAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaag                                                                                                                                       <  1:1131974/140‑2 (MQ=255)
     aGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAggga                                                                                                                                    <  1:878482/140‑2 (MQ=255)
       ggTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGt                                                                                                                                 <  1:2142445/140‑1 (MQ=255)
       ggTATTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGt                                                                                                                                 <  2:1567661/140‑1 (MQ=255)
           tttttATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGACAACAATCAATATCAAATCAGTAGCAAAGAAGGGAGTTAGa                                                                                                                             >  2:1908542/1‑140 (MQ=255)
           tttttATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGa                                                                                                                             <  1:83761/140‑1 (MQ=255)
           tttttATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGa                                                                                                                             <  1:1217236/140‑1 (MQ=255)
           tttttATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGa                                                                                                                             <  2:1037028/140‑1 (MQ=255)
               tATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTg                                                                                                                         <  2:1013834/140‑1 (MQ=255)
                  gTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCAt                                                                                                                      >  1:667907/1‑140 (MQ=255)
                  gTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCAt                                                                                                                      >  2:815888/1‑140 (MQ=255)
                   ttACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATa                                                                                                                     >  2:501673/1‑140 (MQ=255)
                        aTTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGaa                                                                                                                <  1:2306682/140‑1 (MQ=255)
                                gAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCaaaaa                                                                                                        <  2:331592/140‑1 (MQ=255)
                                          aaGATGGATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCAt                                                                                              <  2:189246/140‑1 (MQ=255)
                                           aGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATg                                                                                             <  2:1117819/140‑1 (MQ=255)
                                           aGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATg                                                                                             <  1:1290261/140‑1 (MQ=255)
                                           aGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATg                                                                                             <  1:257998/140‑1 (MQ=255)
                                           aGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATg                                                                                             >  2:620285/1‑140 (MQ=255)
                                           aGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATg                                                                                             <  1:1475275/140‑1 (MQ=255)
                                           aGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATg                                                                                             >  2:297915/1‑140 (MQ=255)
                                           aGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATg                                                                                             >  2:842580/1‑140 (MQ=255)
                                           aGATGTATTTTTGCTTCTGCCCAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAAAAGCAAAGAAGGGATTTATACGTGCATAAAGAAAACAAAAACATATTTCATg                                                                                             >  2:2298114/1‑140 (MQ=255)
                                                tATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGAt                                                                                        >  1:1913724/1‑140 (MQ=255)
                                                     ttGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGt                                                                                   >  1:1811372/1‑140 (MQ=255)
                                                      tGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGtt                                                                                  <  1:835934/140‑1 (MQ=255)
                                                          tCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTgg                                                                              >  1:399605/1‑140 (MQ=255)
                                                           cTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGc                                                                             <  1:701016/140‑1 (MQ=255)
                                                                     acaAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCg                                                                   <  2:92778/140‑1 (MQ=255)
                                                                     acaAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCg                                                                   <  2:1898227/140‑1 (MQ=255)
                                                                             gggggAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATa                                                           <  2:1201654/140‑1 (MQ=255)
                                                                             gggggAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATAGTGCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATa                                                           <  2:469955/140‑1 (MQ=255)
                                                                                   ggTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGACGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGttt                                                     >  1:1142033/1‑140 (MQ=255)
                                                                                   ggTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGttt                                                     >  1:573488/1‑140 (MQ=255)
                                                                                   ggTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGttt                                                     >  2:1736951/1‑140 (MQ=255)
                                                                                        gTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCaa                                                >  1:1934368/1‑140 (MQ=255)
                                                                                            aTGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAg                                            >  2:1170844/1‑140 (MQ=255)
                                                                                              gCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGcc                                          >  1:1043575/1‑140 (MQ=255)
                                                                                              gCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGcc                                          >  2:1609223/1‑140 (MQ=255)
                                                                                              gCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGGTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGcc                                          >  2:1760853/1‑140 (MQ=255)
                                                                                               cTGGAACTCGTGTGAACACAATCACTATCAAATCAATAGCAACGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACAGATTTCATGAAGATCAGGGTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCa                                         <  2:1822701/140‑1 (MQ=255)
                                                                                                 ggAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCCgg                                       >  1:2060182/1‑140 (MQ=255)
                                                                                                 ggAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAgg                                       >  1:1547091/1‑140 (MQ=255)
                                                                                                 ggAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAgg                                       >  1:1833989/1‑140 (MQ=255)
                                                                                                 ggAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGACAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAgg                                       >  2:90974/1‑140 (MQ=255)
                                                                                                   aaCTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTg                                     <  1:296679/140‑1 (MQ=255)
                                                                                                   aaCTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTg                                     <  1:1044581/140‑1 (MQ=255)
                                                                                                   aaCTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTg                                     <  1:1842291/140‑1 (MQ=255)
                                                                                                   aaCTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTg                                     <  1:938488/140‑1 (MQ=255)
                                                                                                    aCTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGc                                    <  2:683601/140‑1 (MQ=255)
                                                                                                         tgtgAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGtttt                               >  2:1011484/1‑140 (MQ=255)
                                                                                                         tgtgAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGGTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGtttt                               >  2:2211027/1‑140 (MQ=255)
                                                                                                                aCAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTg                        <  1:360579/140‑1 (MQ=255)
                                                                                                                aCAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTg                        <  1:452803/140‑1 (MQ=255)
                                                                                                                aCAATCAATATCAAAACACTAGCAAAGAAGGGAGTGAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTg                        <  2:1396126/140‑1 (MQ=255)
                                                                                                                aCAAGCAATATCAACTCAATAGCACAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACAGATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTg                        <  1:2093246/140‑1 (MQ=255)
                                                                                                                    tcaatATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGc                    >  1:730423/1‑140 (MQ=255)
                                                                                                                    tcaatATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGc                    >  2:1084889/1‑140 (MQ=255)
                                                                                                                        tatCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGaa                <  2:2030060/140‑1 (MQ=255)
                                                                                                                            aaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACt            <  1:2182634/140‑1 (MQ=255)
                                                                                                                            aaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACt            <  1:1908670/140‑1 (MQ=255)
                                                                                                                            aaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACt            <  1:898770/140‑1 (MQ=255)
                                                                                                                            aaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACt            <  1:1472577/140‑1 (MQ=255)
                                                                                                                            aaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACt            <  1:1320500/140‑1 (MQ=255)
                                                                                                                            aaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGATGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGTTGCGGAAAACt            <  2:1811372/140‑1 (MQ=255)
                                                                                                                            aaatcaaTAGCAAAGAAGGGAGTTAGACGTGAATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACt            <  1:235051/140‑1 (MQ=255)
                                                                                                                            aaaacaaTAGCAAAGACGGGAGATACACGTCCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACt            <  1:1908542/140‑1 (MQ=255)
                                                                                                                              atcaaTAGCAAAGCAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATGTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGCTGCGGAAAACTAt          <  2:2133668/140‑1 (MQ=255)
                                                                                                                              atcaaTAGCAAAGAAGGGAGTTAGGCGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTAt          >  1:2413097/1‑140 (MQ=255)
                                                                                                                              atcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTAt          <  2:872921/140‑1 (MQ=255)
                                                                                                                              atcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTAt          >  1:2076485/1‑140 (MQ=255)
                                                                                                                              atcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTAt          >  1:529741/1‑140 (MQ=255)
                                                                                                                              atcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTAt          <  2:167173/140‑1 (MQ=255)
                                                                                                                                   tAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGaa                >  1:700500/1‑129 (MQ=255)
                                                                                                                                   tAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGaa                <  2:700500/129‑1 (MQ=255)
                                                                                                                                   tAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGaa                <  2:362372/129‑1 (MQ=255)
                                                                                                                                   tAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGaa                >  1:362372/1‑129 (MQ=255)
                                                                                                                                    aGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTATGTatat    <  1:2229295/140‑1 (MQ=255)
                                                                                                                                    aGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATGTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTATGTatat    <  2:1699892/140‑1 (MQ=255)
                                                                                                                                      cAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTATGTATATGa  <  1:767432/140‑1 (MQ=255)
                                                                                                                                      cAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTATGTATATGa  <  2:2059882/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                       
GCACAAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTATGTATATGA  >  NZ_CP034943/704209‑704483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: