Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,268,704 Δ1 bp 5.8% intergenic (+14/‑186) EO946_RS06595 → / → uxaC NAD(P)/FAD‑dependent oxidoreductase/glucuronate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,268,7040C. 5.8% 343.5 / 18.2 104intergenic (+14/‑186)EO946_RS06595/uxaCNAD(P)/FAD‑dependent oxidoreductase/glucuronate isomerase
Reads supporting (aligned to +/- strand):  ref base C (54/44);  new base . (3/3);  total (57/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

Only 100 of 104 total aligned reads displayed.

GTTGCCATCATTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATAAAGGTAAAATAAAAATAAATCA  >  NZ_CP034943/1268571‑1268842
                                                                                                                                     |                                                                                                                                          
gTTGCCATCATTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGAc                                                                                                                                      <  2:2121562/140‑1 (MQ=255)
gTTGCCATCATTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGAc                                                                                                                                      <  2:1001528/140‑1 (MQ=255)
gTTGCCATCATTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGAc                                                                                                                                      <  2:1685163/140‑1 (MQ=255)
gTTGCCATCATTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGAc                                                                                                                                      <  2:744100/140‑1 (MQ=255)
gTTGCCATCATTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGAc                                                                                                                                      <  2:2288541/140‑1 (MQ=255)
gTTGCCATCATTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGAc                                                                                                                                      <  2:1680290/140‑1 (MQ=255)
gTTGCCATCATTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGAc                                                                                                                                      <  2:321500/140‑1 (MQ=255)
gTTGCCATCATTGGTGCAAAGTCTACACCGCTTAAAGGTCGTCCTGCTTCCTGGATCACAGAAGCAAGTAACGTACGCGACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGAc                                                                                                                                      <  2:339263/140‑1 (MQ=255)
      atcatTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACCTTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGcc                                                                                                                                <  2:606683/140‑1 (MQ=255)
      atcatTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGcc                                                                                                                                <  2:298926/140‑1 (MQ=255)
      atcatTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGCACATTGcc                                                                                                                                <  2:1609886/140‑1 (MQ=255)
      atcatTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTATTTGTGAACTCGGACATTGcc                                                                                                                                <  2:388495/140‑1 (MQ=255)
        catTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCaa                                                                                                                              <  1:1538333/140‑1 (MQ=255)
        catTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCaa                                                                                                                              <  1:2197981/140‑1 (MQ=255)
        catTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCaa                                                                                                                              <  1:1252852/140‑1 (MQ=255)
             gTGCAAATTCTCCACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTATTGTGAACTCGGACATTGCCAAAAgtg                                                                                                                         <  1:213088/140‑1 (MQ=255)
             gTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAgtg                                                                                                                         <  2:2366464/140‑1 (MQ=255)
                 aaaTTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAGGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTcc                                                                                                                     <  2:1779655/140‑1 (MQ=255)
                        acacCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCggg                                                                                                              <  1:328395/140‑1 (MQ=255)
                         cacCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAATGTACGATATTTGACACATATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTAATGAATCTGGCATTGCCAAAAGCATTCCTTTccgagt                                                                                                            >  2:1981214/1‑135 (MQ=255)
                         cacCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAATGTACGATATTTGACACATATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTAATGAATCTGGCATTGCCAAAAGCATTCCTTTccgagt                                                                                                            >  2:1874139/1‑135 (MQ=255)
                         cacCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAATGTACGATATTTGACACATATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTAATGAATCTGGCATTGCCAAAAGCATTCCTTTccgagt                                                                                                            >  2:1683706/1‑135 (MQ=255)
                         cacCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGa                                                                                                             <  1:787256/140‑1 (MQ=255)
                         cacCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGa                                                                                                             <  1:539588/140‑1 (MQ=255)
                         cacCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGa                                                                                                             <  1:2075838/140‑1 (MQ=255)
                              cTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCtt                                                                                                        >  2:375062/1‑140 (MQ=255)
                              cTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCtt                                                                                                        >  2:1631204/1‑140 (MQ=255)
                              cTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCtt                                                                                                        >  1:1370350/1‑140 (MQ=255)
                                       cTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAAt                                                                                               <  1:1398782/140‑1 (MQ=255)
                                       cTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAAt                                                                                               <  1:447508/140‑1 (MQ=255)
                                        ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGTCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                              >  1:25696/1‑140 (MQ=255)
                                        ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGTCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                              >  1:25710/1‑140 (MQ=255)
                                        ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGa                                                                                                             <  2:1865859/125‑1 (MQ=255)
                                        ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGa                                                                                                             >  1:1865859/1‑125 (MQ=255)
                                        ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                              >  1:660574/1‑140 (MQ=255)
                                        ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                              >  1:64070/1‑140 (MQ=255)
                                        ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                              >  2:2152710/1‑140 (MQ=255)
                                        ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                              >  2:1927045/1‑140 (MQ=255)
                                        ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                              >  2:1512847/1‑140 (MQ=255)
                                        ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                              >  1:113109/1‑140 (MQ=255)
                                        ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                              >  1:1386329/1‑140 (MQ=255)
                                        ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                              >  2:2370962/1‑140 (MQ=255)
                                        ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                              >  1:1474854/1‑140 (MQ=255)
                                        ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                              >  1:1650519/1‑140 (MQ=255)
                                        ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                              >  1:157544/1‑140 (MQ=255)
                                        ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                              >  1:1729249/1‑140 (MQ=255)
                                         tCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGt                                                                                             <  2:1370350/140‑1 (MQ=255)
                                                tCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCtttttt                                                                                      >  1:512596/1‑140 (MQ=255)
                                                tCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCtttttt                                                                                      >  1:1244088/1‑140 (MQ=255)
                                                tCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCtttttt                                                                                      >  1:2148494/1‑140 (MQ=255)
                                                        gaAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTa                                                                              <  1:700995/140‑1 (MQ=255)
                                                        gaAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTa                                                                              <  2:1476096/140‑1 (MQ=255)
                                                           agaagCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTg                                                                           >  2:931996/1‑140 (MQ=255)
                                                           agaagCAAGTAACGTACGCTACTTAACTCACAATAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGTACAGTGACAAAAGTGTTCCTTTCGGGATGCTTTACGGCAATGTCTTTTTTTGTTTTTATTg                                                                           >  2:116609/1‑140 (MQ=255)
                                                             aagCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCt                                                                         <  1:2251533/140‑1 (MQ=255)
                                                             aagCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCt                                                                         >  2:1322398/1‑140 (MQ=255)
                                                                   gTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGtt                                                                   >  2:1991388/1‑140 (MQ=255)
                                                                   gTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGtt                                                                   >  2:1631445/1‑140 (MQ=255)
                                                                   gTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGtt                                                                   >  2:1273199/1‑140 (MQ=255)
                                                                             cTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAAcatttcat                                                         <  2:1244088/140‑1 (MQ=255)
                                                                             cTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAAcatttcat                                                         <  2:512596/140‑1 (MQ=255)
                                                                                cTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTCACGTTAACATTTCAtttt                                                      >  1:1633936/1‑140 (MQ=255)
                                                                                cTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCAtttt                                                      >  1:2001668/1‑140 (MQ=255)
                                                                                cTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCAtttt                                                      >  1:1497229/1‑140 (MQ=255)
                                                                                cTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCAtttt                                                      >  1:1254148/1‑140 (MQ=255)
                                                                                cTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCAtttt                                                      >  1:514806/1‑140 (MQ=255)
                                                                                cTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTGTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTGTTATTGCTAACGTTAACATTTCAtttt                                                      >  1:2001611/1‑140 (MQ=255)
                                                                                  tAACTCACATTAAAGGACTTTTCAGACTGGCTTACTAAACCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCAGGCAATGTCTTTTTTTTTTTTTATTGCTAACGTTAACATTTCATTTTCa                                                    <  2:1671933/140‑1 (MQ=255)
                                                                                  tAACTCACATTAAAGGACTTGTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCa                                                    <  2:1671932/140‑1 (MQ=255)
                                                                                   aaCTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCaa                                                   >  1:1605778/1‑140 (MQ=255)
                                                                                        acaTTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAag                                              >  1:457622/1‑140 (MQ=255)
                                                                                        acaTTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAag                                              >  1:2075481/1‑140 (MQ=255)
                                                                                        acaTTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAag                                              >  1:325443/1‑140 (MQ=255)
                                                                                          aTTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGc                                                                                                                                 <  1:1185121/55‑1 (MQ=255)
                                                                                          aTTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGc                                                                                                                                 >  2:1185121/1‑55 (MQ=255)
                                                                                             acacgACTGTTCAGCCTGGCGTACAAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCCGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGAtt                                         <  2:2154652/136‑1 (MQ=255)
                                                                                             aaaGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGAtt                                         <  2:1225926/140‑1 (MQ=255)
                                                                                             aaaGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGAtt                                         <  2:236110/140‑1 (MQ=255)
                                                                                             aaaGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGAtt                                         <  2:641350/140‑1 (MQ=255)
                                                                                                  aCTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATcc                                    <  2:1650519/140‑1 (MQ=255)
                                                                                                     tttCAGCCTGGCTTACTAATCCTTTTAATGAATCTGGCATTGCCAAAAGCATTCCTTTCCGAGTGCTTTCTGGCAATGTCTTTTTTTATGTTTATGACAGCGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCaaag                               <  1:1683706/140‑4 (MQ=255)
                                                                                                     tttCAGCCTGGCTTACTAATCCTTTTAATGAATCTGGCATTGCCAAAAGCATTCCTTTCCGAGTGCTTTCTGGCAATGTCTTTTTTTATGTTTATGACAGCGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCaaag                               <  1:1981214/140‑4 (MQ=255)
                                                                                                     tttCAGCCTGGCTTACTAATCCTTTTAATGAATCTGGCATTGCCAAAAGCATTCCTTTCCGAGTGCTTTCTGGCAATGTCTTTTTTTATGTTTATGACAGCGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCaaag                               <  1:1874139/140‑4 (MQ=255)
                                                                                                      ttCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTCACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCaga                                >  1:662280/1‑140 (MQ=255)
                                                                                                      ttCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCaga                                >  2:545804/1‑140 (MQ=255)
                                                                                                              ggattACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAAtata                        <  2:1200960/137‑1 (MQ=255)
                                                                                                              ggCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAAtata                        <  2:948770/140‑1 (MQ=255)
                                                                                                                cTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATaaa                      >  1:1646355/1‑140 (MQ=255)
                                                                                                                cTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATaaa                      >  1:2125902/1‑140 (MQ=255)
                                                                                                                      aaTCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTCACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATAAAGGtaaa                >  2:976556/1‑140 (MQ=255)
                                                                                                                      aaTCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATAAAGGtaaa                >  2:906930/1‑140 (MQ=255)
                                                                                                                            ttttGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATAAAGTtaaaataaaa          >  2:713244/1‑140 (MQ=255)
                                                                                                                            ttttGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATAAAGGtaaaataaaa          >  2:1085865/1‑140 (MQ=255)
                                                                                                                             tttGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATAAAGGTaaaataaaaa         >  2:1069706/1‑140 (MQ=255)
                                                                                                                               tgtgAACTCGGACATTGCCAATAGTGTTCCTTTCGGGATGCTTGCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCACAGAAGAGATTGATGCCAGAAGAATATAAAGGTaaaataaaaata       >  1:21108/1‑140 (MQ=255)
                                                                                                                               tgtgAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATAAAGGTaaaataaaaata       >  1:2411384/1‑140 (MQ=255)
                                                                                                                               tgtgAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATAAAGGTaaaataaaaata       >  1:685948/1‑140 (MQ=255)
                                                                                                                                    aCTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATAAAGGTAAAATAAAAATAAATCa  <  1:1888319/140‑1 (MQ=255)
                                                                                                                                    aCTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATAAAGGTAAAATAAAAATAAATCa  <  1:2370962/140‑1 (MQ=255)
                                                                                                                                    aCTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATAAAGGTAAAATAAAAATAAATCa  <  1:1512847/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                          
GTTGCCATCATTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATAAAGGTAAAATAAAAATAAATCA  >  NZ_CP034943/1268571‑1268842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: