Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 17,860 C→T 8.5% N239N (AAC→AAT dacA → D‑alanyl‑D‑alanine carboxypeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP03494317,8600CT 8.5% 394.1 / 11.8 147N239N (AAC→AATdacAD‑alanyl‑D‑alanine carboxypeptidase
Reads supporting (aligned to +/- strand):  ref base C (93/34);  new base T (3/9);  total (96/51)
Fisher's exact test for biased strand distribution p-value = 1.35e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 147 total aligned reads displayed.

AGTTTCTGCTAAGGACATGGCAATTCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTACTGTT  >  NZ_CP034943/17725‑17992
                                                                                                                                        |                                                                                                                                    
aGTTTCTGCTAAGGACATGGCAATTCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTa                                                                                                                                  <  1:578016/140‑1 (MQ=255)
aGTTTCTGCTAAGGACATGGCAATTCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTa                                                                                                                                  <  1:681093/140‑1 (MQ=255)
aGTTTCTGCTAAGGACATGGCAATTCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTa                                                                                                                                  <  1:881864/140‑1 (MQ=255)
 gTTTCTGCTAAGGACATGGCAATTCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTAt                                                                                                                                 >  2:846764/1‑140 (MQ=255)
      tGCTAAGGACATGGCAATTCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGa                                                                                                                            <  1:2248446/140‑1 (MQ=255)
      tGCTAAGGACATGGCAATTCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGa                                                                                                                            <  1:1497461/140‑1 (MQ=255)
      tGCTAAGGACATGGCAATTCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGa                                                                                                                            <  1:146288/140‑1 (MQ=255)
      tGCTAAGGACATGGCAATTCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGa                                                                                                                            <  1:932104/140‑1 (MQ=255)
                  ggCAATTCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGTAGACTTCCAGTATCGCTAAAACTAAGTTCAGAGAATGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCa                                                                                                                >  1:389179/1‑140 (MQ=255)
                  ggCAATTCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGACCTTTATGCTGAAAGGGCTTGTCa                                                                                                                >  1:1901767/1‑140 (MQ=255)
                  ggCAATTCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCa                                                                                                                >  1:157187/1‑140 (MQ=255)
                  ggCAATTCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCa                                                                                                                >  1:1790928/1‑140 (MQ=255)
                   gCAATTCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAACCGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAg                                                                                                               <  2:1492646/140‑1 (MQ=255)
                    cAATTCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGc                                                                                                              <  2:191594/140‑1 (MQ=255)
                    cAATTCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGc                                                                                                              <  1:21133/140‑1 (MQ=255)
                    cAATTCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGc                                                                                                              <  2:919797/140‑1 (MQ=255)
                    cAATTCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGc                                                                                                              <  2:2141975/140‑1 (MQ=255)
                    cAATTCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGc                                                                                                              <  1:68045/140‑1 (MQ=255)
                    cAATTCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATACCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGc                                                                                                              <  1:1267262/140‑1 (MQ=255)
                       ttCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAg                                                                                                           >  2:1654031/1‑140 (MQ=255)
                       ttCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAg                                                                                                           >  2:779643/1‑140 (MQ=255)
                       ttCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAg                                                                                                           >  1:2401863/1‑140 (MQ=255)
                       ttCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAg                                                                                                           >  2:2229709/1‑140 (MQ=255)
                       ttCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAg                                                                                                           >  2:1625828/1‑140 (MQ=255)
                       ttCTTGCGGATCACTTGATTACTGACTACCCTGAGATACTGGAGACTTCCAGTATCGCCAAAACGAAATTCAGAGAAGGCACCGATGATGAAATGGACATGCCGAACTGGAATTTTATGCTGAAGGGGCTTGTCAGc                                                                                                              >  2:1626355/1‑137 (MQ=255)
                            gCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAACACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATaa                                                                                                      >  2:2166818/1‑140 (MQ=255)
                            gCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATaa                                                                                                      >  2:1530598/1‑140 (MQ=255)
                             cGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATaaa                                                                                                     >  1:110464/1‑140 (MQ=255)
                             cGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATaaa                                                                                                     >  1:47623/1‑140 (MQ=255)
                                aTCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATaaaaaa                                                                                                  >  1:301036/1‑140 (MQ=255)
                                aTCACTTGATTACTGACTACCATGAGATTCTGGAGACTTCCTGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGTACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTCTaaaaaa                                                                                                  >  1:161661/1‑140 (MQ=255)
                                            cTGACTACCCTGCGATTCTGGACTCTTCAAGTATCGATAAAACCAATTTCACACAAGGCACAGATTATTAAATGCACATGCCGAAGGGGAAATTTAGGCTGAAAGGGGTTGTAAGCGATTATAAAAAAGCGACTGTAGAc                                                                                      <  2:2258580/140‑1 (MQ=255)
                                            cTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGAc                                                                                      >  1:2363778/1‑140 (MQ=255)
                                             tGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACg                                                                                     >  2:1301218/1‑140 (MQ=255)
                                             tGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACg                                                                                     >  1:1657628/1‑140 (MQ=255)
                                             tGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACg                                                                                     >  1:692041/1‑140 (MQ=255)
                                               aCTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGACGTTCAGAGAATGCACCGATGATGAAATGGACATGCCGAACTGGCACTTTATGCTGAAAGGGCTTGTCAGCGCGTCTAAAAAAGCGACTGTAGAgggc                                                                                   >  1:44653/1‑139 (MQ=255)
                                               aCTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGa                                                                                   >  2:153921/1‑140 (MQ=255)
                                               aCTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGa                                                                                   >  2:18289/1‑140 (MQ=255)
                                               aCTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGa                                                                                   >  2:562979/1‑140 (MQ=255)
                                               aCTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGa                                                                                   >  2:1157193/1‑140 (MQ=255)
                                               aCTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGa                                                                                   >  2:430874/1‑140 (MQ=255)
                                               aCTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGa                                                                                   >  1:1516835/1‑140 (MQ=255)
                                               aCTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGa                                                                                   >  2:1711910/1‑140 (MQ=255)
                                               aCTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGa                                                                                   >  2:186490/1‑140 (MQ=255)
                                                 tACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGAtt                                                                                 >  2:1127903/1‑140 (MQ=255)
                                                   cccTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGa                                                                               >  1:1630321/1‑140 (MQ=255)
                                                   cccTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGa                                                                               >  1:1034691/1‑140 (MQ=255)
                                                   cccTGAGATTCTGGAGACTTCCAGAATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTTTAGACGGATTGa                                                                               >  1:2067918/1‑140 (MQ=255)
                                                    ccTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGGACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGaa                                                                              >  2:489583/1‑140 (MQ=255)
                                                    ccTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGaa                                                                              >  2:689099/1‑140 (MQ=255)
                                                    ccTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGaa                                                                              >  2:875032/1‑140 (MQ=255)
                                                       tataTTCTGTATACTTCATGTATCGATAAACCCAAGTTCAGAGACGCAACACTTGATGAAATGCACATGCCTAACTGTAAGTTTATGCTGAACGGGTTTGTCAGCGATTATAAAAAAGCGACTGTAGACGGATTGAAAAc                                                                           <  2:157187/137‑1 (MQ=255)
                                                           ttCTGGAGACTTCCATTATCGATAAACCCAAGTTCATACAAGCACCCGTGTAGTAAAGGCACATGCAGAACTGCAACTTTATGCTCAACGGCCTTGTCAGCGATTATAAAAAAGCGACTGTAGACGGATTGAAAACAgat                                                                       <  2:111976/140‑3 (MQ=255)
                                                           ttCTGCATAATTCCATTTTCGATAAACCCAAGTCCAGAGAAGGCAAATATCATGAAATGGAAATGCCGAAGTGTACCTTTATGCTCACCGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGt                                                                       <  2:1993026/140‑1 (MQ=255)
                                                             cTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTc                                                                     >  1:1005299/1‑140 (MQ=255)
                                                             cTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTc                                                                     >  1:1281825/1‑140 (MQ=255)
                                                             cTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTc                                                                     >  2:576330/1‑140 (MQ=255)
                                                             cTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTc                                                                     >  1:1464143/1‑140 (MQ=255)
                                                             cTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTc                                                                     >  1:2352134/1‑140 (MQ=255)
                                                              tGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCt                                                                    >  1:1893340/1‑140 (MQ=255)
                                                                     ttCCAGTATCGCTAAAACGAAGTTCAGAGACGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGAct                                                             >  2:188944/1‑140 (MQ=255)
                                                                          gTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCg                                                        >  1:1376616/1‑140 (MQ=255)
                                                                          gTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCg                                                        >  2:842444/1‑140 (MQ=255)
                                                                          gTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCg                                                        >  2:1875538/1‑140 (MQ=255)
                                                                               gCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATg                                                                                                                                >  2:346785/1‑63 (MQ=255)
                                                                               gCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATg                                                                                                                                <  1:346785/63‑1 (MQ=255)
                                                                               gCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGACAGGGCTTGTCAGCGAGTAGAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTc                                                   >  2:1594540/1‑140 (MQ=255)
                                                                               gCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTc                                                   >  1:439136/1‑140 (MQ=255)
                                                                               gCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTc                                                   >  1:2231862/1‑140 (MQ=255)
                                                                               gCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTc                                                   >  1:806203/1‑140 (MQ=255)
                                                                               gCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTc                                                   >  2:1611566/1‑140 (MQ=255)
                                                                               gCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTc                                                   >  2:494743/1‑140 (MQ=255)
                                                                                cTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCa                                                  >  2:1613448/1‑140 (MQ=255)
                                                                                        acgTTCAGAGAAGGCACAGATTATGACATGGAACTGCCGAACGGCACCTTTATGCTGAATGGGCTTGTCAGCTATAATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTAc                                          <  2:2292725/138‑1 (MQ=255)
                                                                                         aGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACa                                         >  2:722606/1‑140 (MQ=255)
                                                                                                  ccgccacaGATGATTAAATGTACATGCCGAACTGGATATTTATGCTGAAAGGGTTTTTCCGCGTGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCt                                <  2:245700/136‑1 (MQ=255)
                                                                                                  aaGGCACACCTCTTGAAATGCACATGCCGACCTGGAAGTTTATGCTCACGGGGTTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCt                                <  2:245742/140‑1 (MQ=255)
                                                                                                     gCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGaa                             >  1:2350942/1‑140 (MQ=255)
                                                                                                          gatgatGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCaa                        >  1:1034270/1‑140 (MQ=255)
                                                                                                               atgaAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGa                    >  1:515780/1‑140 (MQ=255)
                                                                                                                 gaAATGGACATGCCGAACTGGAATTTTATGCTGAAGGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGACCAGCTGAACTCAACGGAAt                  >  1:2235375/1‑140 (MQ=255)
                                                                                                                  aattgcaCATCCCCAACTGTAAATTTTTGCTCAACGGGTTTTTCAGCTATTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATg                 <  1:489583/134‑1 (MQ=255)
                                                                                                                  aaaTGCACTTGCCGAACTGGAATTTTTTTCTTAAGTGGCTTGTCAGGTAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATg                 <  2:631070/140‑1 (MQ=255)
                                                                                                                    ttttaCATGCCGAACTGTAATTTTAGGCTGATTGGGCTTGTCAGGCAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCg               <  2:292019/136‑1 (MQ=255)
                                                                                                                    ttGGACATGCCGAACTGGAACTTTTGGCTTAACGGGGTTGTCAGCGATTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCg               <  2:1995988/139‑1 (MQ=255)
                                                                                                                    tgggACATGCCGACCTGGAACTTTTTGCTCAACGGGGTTGTCAGCGTGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCg               <  2:2231862/138‑1 (MQ=255)
                                                                                                                    agggACATGCCGAACTGTAACTTTCTGCTGAACGGGCTTGTCTGCTAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCg               <  2:301036/138‑1 (MQ=255)
                                                                                                                    aTGGACTTGCCTAAGTTGATGTTTATGCTGAAAGGGATTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCg               <  2:1298372/140‑1 (MQ=255)
                                                                                                                     tgcaCAGGCCCAACTGTAATTTTTTGCTCAACGGGTTTGTCCGCGTTTAAAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCgt              <  1:486933/137‑1 (MQ=255)
                                                                                                                      ggACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCgtg             >  2:2230941/1‑140 (MQ=255)
                                                                                                                       tccaggCCCAAGTGCAACTTTATGCTGAACGGGCTTGCCCGCTATTTAAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAAGTCAACGGAATGCgtgt            <  2:418880/135‑1 (MQ=255)
                                                                                                                            gcccaccTGGAACTTTATGCTTAACGGGCTTGACCGCTATTAAAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGttatta       <  2:1282935/134‑1 (MQ=255)
                                                                                                                            gcccaccTGGAAATTTATGCTGAACGGGTGTGTCCGCGATTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGttatta       <  1:1301218/134‑1 (MQ=255)
                                                                                                                            gCCCAACTGGAATTTTTTGCTCACCGGGTTTGTCAGCGTTTAAAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGttatta       <  2:662606/140‑1 (MQ=255)
                                                                                                                            cccGACCTGCACCTTTAGGCGGAACGGGCTTGACAGGTTCAAAAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCCGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGttatta       <  1:1157193/139‑1 (MQ=255)
                                                                                                                             ccGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTAc      >  2:631628/1‑140 (MQ=255)
                                                                                                                             ccGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTAc      >  2:1150225/1‑140 (MQ=255)
                                                                                                                               gAACTTGAAATTTATGCGCACCGGGGTTGTCAGCGTTTATAAAAACGCGACTGGAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTACtg    <  2:2219838/140‑1 (MQ=255)
                                                                                                                                 aCTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTACtgtt  >  2:1489066/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                    
AGTTTCTGCTAAGGACATGGCAATTCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTACTGTT  >  NZ_CP034943/17725‑17992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: