Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 344,594 C→T 5.0% L148L (CTG→TTG)  putR → proline utilization transcriptional regulator PutR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943344,5940CT 5.0% 461.4 / 7.4 138L148L (CTG→TTG) putRproline utilization transcriptional regulator PutR
Reads supporting (aligned to +/- strand):  ref base C (69/62);  new base T (4/3);  total (73/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 138 total aligned reads displayed.

AGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACACCGTGCTGCCTTCCATGTTTTCGGTTGTCA  >  NZ_CP034943/344459‑344729
                                                                                                                                       |                                                                                                                                       
 gTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAg                                                                                                                                    <  1:91775/140‑1 (MQ=255)
              aGGGGAAGTCATGGGCTACATCTGGATTCAGGAGCTGGACCAAAATCTGACGGATGAAGAGCTCGACTTTTTGTACGAGACCTCCTTCCATGTCGGGAAAATCATTTATAAAACGAATAAATTGAAACAGGAAAAGgaag                                                                                                                       >  1:1278619/1‑140 (MQ=255)
              aGGGGAAGTCATGGGCTACATCTGGATTCAGGAGCTGGACCAAAATCTGACGGATGAAGAGCTCGACTTTTTGTACGAGACCTCCTTCCATGTCGGGAAAATCATTTATAAAACGAATAAATTGAAACAGGAAAAGgaag                                                                                                                       >  1:1278980/1‑140 (MQ=255)
              aGGGGAAGTCATGGGCTACATCTGGATTCAGGAGCTGGACCAAAATCTGACGGATGAAGAGCTCGACTTTTTGTACGAGACCTCCTTCCATGTCGGGAAAATCATTTATAAAACGAATAAATTGAAACAGGAAAAGgaag                                                                                                                       >  1:512300/1‑140 (MQ=255)
               ggggAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATTTGGGTAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGgaaga                                                                                                                      <  1:332783/140‑1 (MQ=255)
                  gAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGaaaa                                                                                                                   >  1:1212449/1‑140 (MQ=255)
                   aaGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGaaaaa                                                                                                                  >  1:1352800/1‑140 (MQ=255)
                   aaGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGaaaaa                                                                                                                  >  1:2087031/1‑140 (MQ=255)
                    aGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGACCTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGACAAGGAAGAACAAg                                                                                                                 >  2:1550829/1‑140 (MQ=255)
                    aGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAg                                                                                                                 >  2:508645/1‑140 (MQ=255)
                    aGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAg                                                                                                                 >  2:1981343/1‑140 (MQ=255)
                    aGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAg                                                                                                                 >  2:1213220/1‑140 (MQ=255)
                       cATGGGCTACATCTTGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGTACTGGTCTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCTTTTATAAAACGACCAAGCTGAAGCAGGAAAAGGTAGAAAAAGCgg                                                                                                              <  2:1653395/140‑1 (MQ=255)
                          gggCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGCAAAGGAAGAAAAAGCGGAAg                                                                                                           >  2:301548/1‑140 (MQ=255)
                          gggCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAg                                                                                                           >  2:129322/1‑140 (MQ=255)
                            gatACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGAt                                                                                                         <  2:2237701/138‑1 (MQ=255)
                            gCTACATCTGGGTTCAGGAGCTGGACCAAAATTTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGAt                                                                                                         <  2:146890/140‑1 (MQ=255)
                            gCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGAt                                                                                                         <  2:1992608/140‑1 (MQ=255)
                            gCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGAt                                                                                                         <  1:1648870/140‑1 (MQ=255)
                            gCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGAt                                                                                                         <  2:1422390/140‑1 (MQ=255)
                            gCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGAt                                                                                                         <  2:2398903/140‑1 (MQ=255)
                            gCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGAt                                                                                                         <  1:256806/140‑1 (MQ=255)
                               aCATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGAtctc                                                                                                      >  1:1353455/1‑140 (MQ=255)
                               aCATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGAtctc                                                                                                      >  1:2306252/1‑140 (MQ=255)
                                cATCTGGGTTCAGGAGCTGGAGCAAAATCTGACGGAAGAAGTACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAATTCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGAGCTCa                                                                                                     >  1:1653395/1‑140 (MQ=255)
                                cATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGACAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGACGATCTCa                                                                                                     >  2:1517513/1‑140 (MQ=255)
                                cATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCgg                                                                                                              >  1:141672/1‑131 (MQ=255)
                                cATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCa                                                                                                     >  2:1754820/1‑140 (MQ=255)
                                cATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCa                                                                                                     >  2:166829/1‑140 (MQ=255)
                                cATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCa                                                                                                     >  2:804740/1‑140 (MQ=255)
                                     gggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTaaa                                                                                                <  1:588675/140‑1 (MQ=255)
                                      ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGACAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAAc                                                                                               >  2:2323991/1‑140 (MQ=255)
                                      ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGaaa                                                                                                                    >  2:1298617/1‑119 (MQ=255)
                                      ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGaaa                                                                                                                    <  1:1298617/119‑1 (MQ=255)
                                      ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAAc                                                                                               >  2:1482957/1‑140 (MQ=255)
                                      ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAAc                                                                                               >  2:1915116/1‑140 (MQ=255)
                                      ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAAc                                                                                               >  2:53412/1‑140 (MQ=255)
                                      ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAAc                                                                                               >  2:2049134/1‑140 (MQ=255)
                                      ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAACGAAGAAAAAGCGGAAGATCACATTAAAc                                                                                               >  2:416637/1‑140 (MQ=255)
                                      ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGGCGGGAAAATCATTGATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAAc                                                                                               >  2:1162843/1‑140 (MQ=255)
                                         tCAGGAGCTGGACCAAAATCTGAGGGAAGAAGAACTAGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACggg                                                                                            <  2:1357432/140‑1 (MQ=255)
                                         tCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACggg                                                                                            <  1:257015/140‑1 (MQ=255)
                                         tCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACggg                                                                                            <  2:2182117/140‑1 (MQ=255)
                                          cAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGc                                                                                           <  2:2361675/140‑1 (MQ=255)
                                          cAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGc                                                                                           <  2:1086800/140‑1 (MQ=255)
                                           aGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGACAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCg                                                                                          >  1:1489631/1‑140 (MQ=255)
                                           aGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGc                                                                                           <  1:1898390/139‑1 (MQ=255)
                                           aGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGc                                                                                           >  2:1898390/1‑139 (MQ=255)
                                           aGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCg                                                                                          >  1:1185537/1‑140 (MQ=255)
                                           aGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCg                                                                                          >  2:455489/1‑140 (MQ=255)
                                           aGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCg                                                                                          >  1:2186428/1‑140 (MQ=255)
                                            ggAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGa                                                                                         <  2:1943849/140‑1 (MQ=255)
                                               gCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCt                                                                                      <  2:1185537/140‑1 (MQ=255)
                                                  ggACCAAAATCTGACGGACGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGAGGAAGATCTCATTAAACGGGCGATCTATc                                                                                   >  2:1599909/1‑140 (MQ=255)
                                                  ggACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATc                                                                                   >  1:781061/1‑140 (MQ=255)
                                                  ggACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATc                                                                                   >  1:2111707/1‑140 (MQ=255)
                                                  ggACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGAAGGAAAAGGAAGAAAAAGCGGTAGATCTCATTAAAAGGGAGAtctctc                                                                                   >  2:2035925/1‑140 (MQ=255)
                                                     ccAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGc                                                                                <  1:1365925/140‑1 (MQ=255)
                                                        aaaTCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAAt                                                                             >  2:1730250/1‑140 (MQ=255)
                                                        aaaTCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAAt                                                                             >  2:13114/1‑140 (MQ=255)
                                                           tCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTa                                                                          <  1:1244396/140‑1 (MQ=255)
                                                           tCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTa                                                                          <  2:1575945/140‑1 (MQ=255)
                                                              gACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCa                                                                       >  2:832963/1‑140 (MQ=255)
                                                               aCGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCAc                                                                      <  1:1443870/140‑1 (MQ=255)
                                                                cGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACt                                                                     <  1:455489/140‑1 (MQ=255)
                                                                 ggAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTg                                                                    <  2:1454403/140‑1 (MQ=255)
                                                                    agaagaaCTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTgaga                                                                 <  1:1572318/140‑1 (MQ=255)
                                                                                cTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGAc                                                     >  1:217623/1‑140 (MQ=255)
                                                                                cTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGAc                                                     >  1:723/1‑140 (MQ=255)
                                                                                   tttGTACGAGACCTCCTTCCATGTCGGGAAAATCATTTATAAAACGAATAAATTGAAACAGGAAAAGGAAGAAAAAGCAGAGGATCTCATCAAACGGGCGATCTATCAGCAATTTACCTCTGAGAAGGAACTCAGACggg                                                  <  2:512300/140‑1 (MQ=255)
                                                                                   tttGTACGAGACCTCCTTCCATGTCGGGAAAATCATTTATAAAACGAATAAATTGAAACAGGAAAAGGAAGAAAAAGCAGAGGATCTCATCAAACGGGCGATCTATCAGCAATTTACCTCTGAGAAGGAACTCAGACggg                                                  <  2:1278980/140‑1 (MQ=255)
                                                                                     tGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGaa                                                >  1:875506/1‑140 (MQ=255)
                                                                                          gagaCCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGa                                           >  2:804530/1‑140 (MQ=255)
                                                                                          gagaCCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGa                                           >  2:233247/1‑140 (MQ=255)
                                                                                           agaCCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGaa                                          <  1:1780566/140‑1 (MQ=255)
                                                                                             aCCTCTTTTCATGTCGTGAAAATCATTTATAAAAAGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAg                                        <  2:1441695/140‑1 (MQ=255)
                                                                                             aCCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAg                                        <  2:1695558/140‑1 (MQ=255)
                                                                                             aCCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAg                                        >  2:963355/1‑140 (MQ=255)
                                                                                             aCCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAg                                        >  2:963105/1‑140 (MQ=255)
                                                                                               ctctTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGaa                                      >  2:1781053/1‑140 (MQ=255)
                                                                                                   tttCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCaa                                  <  1:508645/140‑1 (MQ=255)
                                                                                                    ttCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCAGAATATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAac                                 >  2:1154434/1‑140 (MQ=255)
                                                                                                    ttCATGTCGGGAAAATCAGTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCAATGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAac                                 >  2:2414198/1‑140 (MQ=255)
                                                                                                     tCATTTCGTGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAaca                                <  2:1331248/140‑1 (MQ=255)
                                                                                                     tCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAaca                                <  1:832963/140‑1 (MQ=255)
                                                                                                     tCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAaca                                <  1:963355/140‑1 (MQ=255)
                                                                                                         gTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGACGCCGAAAGAATCAACACCGt                            >  1:1968478/1‑140 (MQ=255)
                                                                                                           cGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAACAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACACCGtgc                          <  1:1299544/140‑1 (MQ=255)
                                                                                                           cGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACACCGtgc                          <  1:2274684/140‑1 (MQ=255)
                                                                                                           cGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACACCGtgc                          <  1:1299622/140‑1 (MQ=255)
                                                                                                            gggAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACACCGtgct                         >  2:752874/1‑140 (MQ=255)
                                                                                                                   tCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTACACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGACTCAACACCGTGCTGCCTTcc                  <  1:260455/140‑1 (MQ=255)
                                                                                                                           aaaaCGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACACCGTGCTGCCTTCCATGTTTTc          <  2:1457787/140‑1 (MQ=255)
                                                                                                                             aaCGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACACCGTGCTGCCTTCCATGTTTTCgg        >  1:2263217/1‑140 (MQ=255)
                                                                                                                             aaCGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACACCGTGCTGCCTTCCATGTTTTCgg        >  1:320504/1‑140 (MQ=255)
                                                                                                                             aaCGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACACCGTGCTGCCTTCCATGTTTTCgg        <  2:2186428/140‑1 (MQ=255)
                                                                                                                             aaCGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACACCGTGCTGCCTTCCATGTGTTCgg        >  1:1458534/1‑140 (MQ=255)
                                                                                                                                gAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACACCGTGCTGCCTTCCATGTTTTCGGTTg     <  1:1482957/140‑1 (MQ=255)
                                                                                                                                   cAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACACCGTGCTGCCTTCCATGTTTTCGGTTGTCa  <  2:716562/140‑1 (MQ=255)
                                                                                                                                   cAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACACCGTGCTGCCTTCCATGTTTTCGGTTGTCa  <  2:1019004/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                       
AGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACACCGTGCTGCCTTCCATGTTTTCGGTTGTCA  >  NZ_CP034943/344459‑344729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: