Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,761,518 T→A 6.1% N9K (AAT→AAA) ‡ EO946_RS09125 → non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,761,5180TA 6.1% 378.0 / 12.0 115N9K (AAT→AAA) ‡EO946_RS09125non‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base T (36/72);  new base A (5/2);  total (41/74)
Fisher's exact test for biased strand distribution p-value = 9.54e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 119 total aligned reads displayed.

AAATGCATGAGAAAACATTTCATCATGACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAA  >  NZ_CP034943/1761379‑1761657
                                                                                                                                           |                                                                                                                                           
aaaTGCCTGAGAAAACAGTTCATCATGACGAAGTCAAAACGAGAAGCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAAGAATGATAATACCCGCAAt                                                                                                                                             <  2:1163923/140‑1 (MQ=255)
aaaTGCATGAGAAACCATTTCATCATGACTAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACGGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAAt                                                                                                                                             <  1:1575052/140‑1 (MQ=255)
aaaTGCATGAGAAAACATTTCATCATGACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAAt                                                                                                                                             <  1:1758281/140‑1 (MQ=255)
   tGCATGAGAAAACATTTCATCATGACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTa                                                                                                                                          >  2:1120059/1‑140 (MQ=255)
   tGCATGAGAAAACATTTCATCATGACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTa                                                                                                                                          >  2:2083190/1‑140 (MQ=255)
   tGCATGAGAAAACATTTCATCATGACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTa                                                                                                                                          >  2:2206228/1‑140 (MQ=255)
     cATGAGAAAACATTTCATCATGACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATCAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGc                                                                                                                                        <  2:805126/140‑1 (MQ=255)
     cATGAGAAAACATTTCATCATGACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGc                                                                                                                                        >  1:106018/1‑140 (MQ=255)
     cATGAGAAAACATTTCATCATGACGAAGTCAAAACGAGAATCAAAGGTTGGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGc                                                                                                                                        <  2:1454092/140‑1 (MQ=255)
          taaaaCATTTCATCATGACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCTCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATc                                                                                                                                   <  2:614410/139‑1 (MQ=255)
          gaAAACATTTCATCATGACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATc                                                                                                                                   <  2:686245/140‑1 (MQ=255)
             aaCATTTCATCATGACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCtt                                                                                                                                >  1:1095251/1‑140 (MQ=255)
                 tttCATCATGACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGt                                                                                                                            <  1:1397785/140‑1 (MQ=255)
                 tttCATCATGACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGt                                                                                                                            <  1:1399154/140‑1 (MQ=255)
                 tttCATCATGACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGt                                                                                                                            <  1:2355055/140‑1 (MQ=255)
                 tttCATCATGACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGt                                                                                                                            <  1:322517/140‑1 (MQ=255)
                 tgtcatcaTGACGAAGTCAAAACGAGAATCAAAGGGTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGt                                                                                                                            <  2:177636/138‑1 (MQ=255)
                  ttCATCATGACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTc                                                                                                                           >  2:643540/1‑140 (MQ=255)
                     atcatGACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCCGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGc                                                                                                                        >  2:2428514/1‑140 (MQ=255)
                     atcatGACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGc                                                                                                                        >  2:1763586/1‑140 (MQ=255)
                          gACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATgg                                                                                                                   >  1:1091138/1‑140 (MQ=255)
                          gACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATgg                                                                                                                   >  1:746080/1‑140 (MQ=255)
                          gACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATgg                                                                                                                   >  2:256969/1‑140 (MQ=255)
                          gACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATgg                                                                                                                   >  1:1439561/1‑140 (MQ=255)
                            cGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTg                                                                                                                 <  2:839290/140‑1 (MQ=255)
                            cGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTg                                                                                                                 <  1:643540/140‑1 (MQ=255)
                            cGAAGTCAAAACCAGAATCAAAGCGTTGTATGGAAATTAACTGAGAATCTACATAAAACTGCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTg                                                                                                                 <  1:1510800/140‑1 (MQ=255)
                              aaGTCAAAACGAGAATCAAAGGTGTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTgag                                                                                                               <  1:2402638/140‑1 (MQ=255)
                               aGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTgaga                                                                                                              >  2:2196134/1‑140 (MQ=255)
                               aGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTgaga                                                                                                              >  2:502482/1‑140 (MQ=255)
                                gTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGAc                                                                                                             >  2:1725502/1‑140 (MQ=255)
                                 tCAAAACGAGAATCAAAGGTTTTTATGGAAATTAACTGAGAATCTACATAAAAATTCGCATGTGAAATGGGGGCAGCGCGATGAGAAATAATGATACTACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                            <  2:697174/140‑1 (MQ=255)
                                 tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                            <  2:2238289/140‑1 (MQ=255)
                                 tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                            <  2:106018/140‑1 (MQ=255)
                                 tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                            <  1:745679/140‑1 (MQ=255)
                                 tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                            <  1:662600/140‑1 (MQ=255)
                                 tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                            <  1:389985/140‑1 (MQ=255)
                                 tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                            <  1:369844/140‑1 (MQ=255)
                                 tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                            <  1:151545/140‑1 (MQ=255)
                                 tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                            <  1:1547149/140‑1 (MQ=255)
                                 tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                            <  1:1175313/140‑1 (MQ=255)
                                   aaaaCGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACttt                                                                                                          >  2:614879/1‑140 (MQ=255)
                                   aaaaCGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACttt                                                                                                          >  2:130301/1‑140 (MQ=255)
                                    aaccGAGACTCACAGGCTTGTAGGGAAAGTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTa                                                                                                         <  2:1977143/137‑1 (MQ=255)
                                      aCGAGAATCAAAGGTTTGTATGGAAATTAACTGAGGATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACa                                                                                                       <  1:1869727/140‑1 (MQ=255)
                                      aCGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACa                                                                                                       <  1:1546630/140‑1 (MQ=255)
                                      aCGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACa                                                                                                       <  1:1722709/140‑1 (MQ=255)
                                      aCGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACa                                                                                                       <  2:1596459/140‑1 (MQ=255)
                                      aCGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTACCTAATCCTTCTGTCAGCTATGGTGAGACTTTACa                                                                                                       <  1:2032995/140‑1 (MQ=255)
                                         agaATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACatat                                                                                                    <  1:683719/140‑1 (MQ=255)
                                           aaTCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACatatat                                                                                                  >  1:80605/1‑140 (MQ=255)
                                           aaTCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACatatat                                                                                                  >  1:1749464/1‑140 (MQ=255)
                                              cAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTg                                                                                               <  1:2212998/140‑1 (MQ=255)
                                              cAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTg                                                                                               <  1:719610/140‑1 (MQ=255)
                                              cAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTg                                                                                               <  2:1439561/140‑1 (MQ=255)
                                                    tttGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGagc                                                                                         <  2:1002732/140‑1 (MQ=255)
                                                    tttGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGagc                                                                                         <  2:1520481/140‑1 (MQ=255)
                                                    tttGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGagc                                                                                         <  2:1520443/140‑1 (MQ=255)
                                                         aTGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGCTGAGAAATAATGATCAGACCCGCAATTTAGCTAATCCTACTGTCAGCTCTGGTGAGACTTTACATATATCTGAAGAGCAGCCg                                                                                    <  1:2428514/140‑1 (MQ=255)
                                                         aTGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCg                                                                                    <  1:1763586/140‑1 (MQ=255)
                                                                      gcccaTCTACATAAACATTCCCAGGAGAAATGGGCGCAGCGCGATGAGAAATAATGATAATACCCGCACTTTAGCTACTCCTTCTGTCAGCTATGGTGAGACTTTACATATCTCTGAAGAGCAGCCGGCAACGATACCTg                                                                       <  2:1254470/136‑1 (MQ=255)
                                                                      gagaCTCTACATAAAACTTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAAGACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                       <  2:1987399/140‑1 (MQ=255)
                                                                      gagaATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                       <  2:1406847/140‑1 (MQ=255)
                                                                      gagaATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                       <  2:1347649/140‑1 (MQ=255)
                                                                      gagaATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                       <  2:1531585/140‑1 (MQ=255)
                                                                      gagaATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                       <  1:1107516/140‑1 (MQ=255)
                                                                      gagaATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                       <  1:502482/140‑1 (MQ=255)
                                                                           tCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGtt                                                                  <  2:80605/140‑1 (MQ=255)
                                                                              aCATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTta                                                               >  2:2070218/1‑140 (MQ=255)
                                                                              aCAGAAAACTTCGCAGGTGAAAGGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTGTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTta                                                               >  2:461013/1‑140 (MQ=255)
                                                                                aTAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTtata                                                             <  2:1679268/140‑1 (MQ=255)
                                                                                    aaaTTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCACCGATACCTGAAGTTTTATACAGa                                                         >  2:1442300/1‑140 (MQ=255)
                                                                                    aaaTTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGa                                                         >  2:505178/1‑140 (MQ=255)
                                                                                     attttgCAGGTGAAATGGAGGCAGCGTGATGAGAAATAATGATAATATCCGCATATTAACTAATCCTTCTGTCAGTCATGGGGAGCCTTTACATATATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGaa                                                        >  1:2045715/6‑140 (MQ=255)
                                                                                     attttgCAGGTGAAATGGAGGCAGCGTGATGAGAAATAATGATAATATCCGCATATTAACTAATCCTTCTGTCAGTCATGGGGAGCCTTTACATATATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGaa                                                        >  1:1995656/6‑140 (MQ=255)
                                                                                             ggTGAAATGGGGGCAGCGCGATGAGAAATACTGATAATACCCGCAATTTAGCTAATCCTTCTGGCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTATTATACAGAACGGCTGCg                                                <  2:145985/140‑1 (MQ=255)
                                                                                             ggTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                <  1:2083190/140‑1 (MQ=255)
                                                                                             ggTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                <  2:2431368/140‑1 (MQ=255)
                                                                                             ggTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                <  1:2206228/140‑1 (MQ=255)
                                                                                             ggTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                <  1:951635/140‑1 (MQ=255)
                                                                                             ggTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                <  1:1120059/140‑1 (MQ=255)
                                                                                             ggTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                <  2:438135/140‑1 (MQ=255)
                                                                                             ggTGAAATGGAGGCAGCGTGATGAGAAATAATGATAATATCCGCATATTAACTAATCCTTCTGTCAGTCATGGGGAGCCTTTACATATATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGAACGGcgacg                                                <  2:1995656/140‑5 (MQ=255)
                                                                                                  aaTGGGGCCAGCGCGATTAGACATAATGATAATACCCGCAATATAGATAATCCTTCAGTCAGATAGGGTGAGACTTTACAAATATCTGAAGATCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGACCt                                           >  2:578821/1‑140 (MQ=255)
                                                                                                    tGGGGGCAGCGCGATGAGAAATACTGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTg                                         <  2:253692/140‑1 (MQ=255)
                                                                                                    tGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTg                                         <  2:928270/140‑1 (MQ=255)
                                                                                                    tGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTg                                         <  2:350689/140‑1 (MQ=255)
                                                                                                    tGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTg                                         <  2:1706537/140‑1 (MQ=255)
                                                                                                     gggggCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAAGCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTgg                                        <  1:1278560/140‑1 (MQ=255)
                                                                                                             cgtgATGAGAAATAATGATAATATCCGCATATTAACTAATCCTTCTGTCAGTCATGGGGAGCCTTTACATATATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGAACGGCGACGGAGCTTGGGGATACaa                                >  1:772092/4‑140 (MQ=255)
                                                                                                             cgtgATGAGAAATAATGATAATATCCGCATATTAACTAATCCTTCTGTCAGTCATGGGGAGCCTTTACATATATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGAACGGCGACGGAGCTTGGGGATACaa                                >  1:522254/4‑140 (MQ=255)
                                                                                                             cgcgATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACaa                                >  2:495451/1‑140 (MQ=255)
                                                                                                                              ataataCCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTg               <  1:1679891/140‑1 (MQ=255)
                                                                                                                              ataataCCCGCAATTTAGCTAAGCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTg               <  1:124091/140‑1 (MQ=255)
                                                                                                                                aataCCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTTTACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCa             >  2:32709/1‑140 (MQ=255)
                                                                                                                                aataCCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTGACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCATGGGGAGACAAAGAGACTCATTTATTTGCa             >  2:1358484/1‑140 (MQ=255)
                                                                                                                                      cGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGa       <  1:1652734/140‑1 (MQ=255)
                                                                                                                                       gCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGAt      >  1:647813/1‑140 (MQ=255)
                                                                                                                                        cAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATg     >  1:855820/1‑140 (MQ=255)
                                                                                                                                           tttAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTTTACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaa  <  1:32709/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
AAATGCATGAGAAAACATTTCATCATGACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAA  >  NZ_CP034943/1761379‑1761657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: