Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,761,522 G→A 6.2% A11T (GCT→ACT)  EO946_RS09125 → non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,761,5220GA 6.2% 363.1 / 8.1 114A11T (GCT→ACT) EO946_RS09125non‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base G (32/74);  new base A (5/2);  total (37/77)
Fisher's exact test for biased strand distribution p-value = 3.70e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

CATGAGAAAACATTTCATCATGACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTG  >  NZ_CP034943/1761384‑1761660
                                                                                                                                          |                                                                                                                                          
cttGATAAAACTTTTCATAATGACTAAGTCAAAACGAGAATCATAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGAGATGAGAAATAATGATAAGACCCGCAATTTAGc                                                                                                                                           <  2:1416516/138‑1 (MQ=255)
cATGAGAAAACATTTCATCATGACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATCAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGc                                                                                                                                           <  2:805126/140‑1 (MQ=255)
cATGAGAAAACATTTCATCATGACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGc                                                                                                                                           >  1:106018/1‑140 (MQ=255)
cATGAGAAAACATTTCATCATGACGAAGTCAAAACGAGAATCAAAGGTTGGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGc                                                                                                                                           <  2:1454092/140‑1 (MQ=255)
     taaaaCATTTCATCATGACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCTCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATc                                                                                                                                      <  2:614410/139‑1 (MQ=255)
     gaAAACATTTCATCATGACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATc                                                                                                                                      <  2:686245/140‑1 (MQ=255)
        aaCATTTCATCATGACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCtt                                                                                                                                   >  1:1095251/1‑140 (MQ=255)
            tttCATCATGACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGt                                                                                                                               <  1:1397785/140‑1 (MQ=255)
            tttCATCATGACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGt                                                                                                                               <  1:1590973/140‑1 (MQ=255)
            tttCATCATGACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGt                                                                                                                               <  1:322517/140‑1 (MQ=255)
            tttCATCATGACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGt                                                                                                                               <  1:246453/140‑1 (MQ=255)
            tttCATCATGACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGt                                                                                                                               <  1:1399154/140‑1 (MQ=255)
            tttCATCATGACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGt                                                                                                                               <  1:2355055/140‑1 (MQ=255)
             ttCATCATGACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTc                                                                                                                              >  2:643540/1‑140 (MQ=255)
                atcatGACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCCGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGc                                                                                                                           >  2:2428514/1‑140 (MQ=255)
                atcatGACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGc                                                                                                                           >  2:1763586/1‑140 (MQ=255)
                     gACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATgg                                                                                                                      >  2:256969/1‑140 (MQ=255)
                     gACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATgg                                                                                                                      >  1:746080/1‑140 (MQ=255)
                     gACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATgg                                                                                                                      >  1:1439561/1‑140 (MQ=255)
                     gACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATgg                                                                                                                      >  1:1091138/1‑140 (MQ=255)
                       cGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTg                                                                                                                    <  1:643540/140‑1 (MQ=255)
                       cGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTg                                                                                                                    <  2:839290/140‑1 (MQ=255)
                       cGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTg                                                                                                                    <  1:1510667/140‑1 (MQ=255)
                       cGAAGTCAAAACCAGAATCAAAGCGTTGTATGGAAATTAACTGAGAATCTACATAAAACTGCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTg                                                                                                                    <  1:1510800/140‑1 (MQ=255)
                         aaGTCAAAACGAGAATCAAAGGTGTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTgag                                                                                                                  <  1:2402638/140‑1 (MQ=255)
                          aGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTgaga                                                                                                                 >  2:2196134/1‑140 (MQ=255)
                          aGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTgaga                                                                                                                 >  2:502482/1‑140 (MQ=255)
                           gTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGAc                                                                                                                >  2:1725502/1‑140 (MQ=255)
                            tCAAAACGAGAATCAAAGGTTTTTATGGAAATTAACTGAGAATCTACATAAAAATTCGCATGTGAAATGGGGGCAGCGCGATGAGAAATAATGATACTACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                               <  2:697174/140‑1 (MQ=255)
                            tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                               <  1:745679/140‑1 (MQ=255)
                            tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                               <  1:151545/140‑1 (MQ=255)
                            tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                               <  1:1547149/140‑1 (MQ=255)
                            tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                               <  2:106018/140‑1 (MQ=255)
                            tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                               <  2:2238289/140‑1 (MQ=255)
                            tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                               <  1:662600/140‑1 (MQ=255)
                            tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                               <  1:389985/140‑1 (MQ=255)
                            tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                               <  1:1175313/140‑1 (MQ=255)
                              aaaaCGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACttt                                                                                                             >  2:130301/1‑140 (MQ=255)
                               aaccGAGACTCACAGGCTTGTAGGGAAAGTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTa                                                                                                            <  2:1977143/137‑1 (MQ=255)
                                 aCGAGAATCAAAGGTTTGTATGGAAATTAACTGAGGATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACa                                                                                                          <  1:1869727/140‑1 (MQ=255)
                                 aCGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACa                                                                                                          <  2:1596459/140‑1 (MQ=255)
                                 aCGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACa                                                                                                          <  1:1722709/140‑1 (MQ=255)
                                 aCGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTACCTAATCCTTCTGTCAGCTATGGTGAGACTTTACa                                                                                                          <  1:2032995/140‑1 (MQ=255)
                                      aaTCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACatatat                                                                                                     >  1:1749464/1‑140 (MQ=255)
                                      aaTCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACatatat                                                                                                     >  1:80605/1‑140 (MQ=255)
                                         cAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTg                                                                                                  <  2:1439561/140‑1 (MQ=255)
                                         cAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTg                                                                                                  <  1:719610/140‑1 (MQ=255)
                                         cAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTg                                                                                                  <  1:2212998/140‑1 (MQ=255)
                                               tttGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGagc                                                                                            <  2:365083/140‑1 (MQ=255)
                                               tttGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGagc                                                                                            <  2:1520481/140‑1 (MQ=255)
                                               tttGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGagc                                                                                            <  2:1520443/140‑1 (MQ=255)
                                               tttGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGagc                                                                                            <  2:1002732/140‑1 (MQ=255)
                                                    aTGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGCTGAGAAATAATGATCAGACCCGCAATTTAGCTAATCCTACTGTCAGCTCTGGTGAGACTTTACATATATCTGAAGAGCAGCCg                                                                                       <  1:2428514/140‑1 (MQ=255)
                                                    aTGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCg                                                                                       <  1:1763586/140‑1 (MQ=255)
                                                                 gcccaTCTACATAAACATTCCCAGGAGAAATGGGCGCAGCGCGATGAGAAATAATGATAATACCCGCACTTTAGCTACTCCTTCTGTCAGCTATGGTGAGACTTTACATATCTCTGAAGAGCAGCCGGCAACGATACCTg                                                                          <  2:1254470/136‑1 (MQ=255)
                                                                 gagaCTCTACATAAAACTTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAAGACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                          <  2:1987399/140‑1 (MQ=255)
                                                                 gagaATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                          <  1:502482/140‑1 (MQ=255)
                                                                 gagaATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                          <  2:1531585/140‑1 (MQ=255)
                                                                 gagaATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                          <  1:1107516/140‑1 (MQ=255)
                                                                 gagaATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                          <  2:1347649/140‑1 (MQ=255)
                                                                 gagaATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                          <  1:2196134/140‑1 (MQ=255)
                                                                      tCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGtt                                                                     <  2:80605/140‑1 (MQ=255)
                                                                         aCATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTta                                                                  >  2:2070218/1‑140 (MQ=255)
                                                                         aCAGAAAACTTCGCAGGTGAAAGGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTGTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTta                                                                  >  2:461013/1‑140 (MQ=255)
                                                                           aTAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTtata                                                                <  2:1679268/140‑1 (MQ=255)
                                                                             aaaaaTTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACa                                                              >  1:1207098/1‑140 (MQ=255)
                                                                               aaaTTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCACCGATACCTGAAGTTTTATACAGa                                                            >  2:1442300/1‑140 (MQ=255)
                                                                               aaaTTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGa                                                            >  2:505178/1‑140 (MQ=255)
                                                                                attttgCAGGTGAAATGGAGGCAGCGTGATGAGAAATAATGATAATATCCGCATATTAACTAATCCTTCTGTCAGTCATGGGGAGCCTTTACATATATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGaa                                                           >  1:1995656/6‑140 (MQ=255)
                                                                                attttgCAGGTGAAATGGAGGCAGCGTGATGAGAAATAATGATAATATCCGCATATTAACTAATCCTTCTGTCAGTCATGGGGAGCCTTTACATATATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGaa                                                           >  1:2045715/6‑140 (MQ=255)
                                                                                        ggTGAAATGGGGGCAGCGCGATGAGAAATACTGATAATACCCGCAATTTAGCTAATCCTTCTGGCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTATTATACAGAACGGCTGCg                                                   <  2:145985/140‑1 (MQ=255)
                                                                                        ggTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                   <  1:1120059/140‑1 (MQ=255)
                                                                                        ggTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                   <  1:2083190/140‑1 (MQ=255)
                                                                                        ggTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                   <  1:951635/140‑1 (MQ=255)
                                                                                        ggTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                   <  2:2431368/140‑1 (MQ=255)
                                                                                        ggTGAAATGGAGGCAGCGTGATGAGAAATAATGATAATATCCGCATATTAACTAATCCTTCTGTCAGTCATGGGGAGCCTTTACATATATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGAACGGcgacg                                                   <  2:1995656/140‑5 (MQ=255)
                                                                                        ggTGAAATGGAGGCAGCGTGATGAGAAATAATGATAATATCCGCATATTAACTAATCCGTCTGTCAGTCATGGGGAGCCTTTACATATATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGAACGGcgacg                                                   <  2:2045715/140‑5 (MQ=255)
                                                                                             aaTGGGGCCAGCGCGATTAGACATAATGATAATACCCGCAATATAGATAATCCTTCAGTCAGATAGGGTGAGACTTTACAAATATCTGAAGATCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGACCt                                              >  2:578821/1‑140 (MQ=255)
                                                                                               tGGGGGCAGCGCGATGAGAAATACTGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTg                                            <  2:253692/140‑1 (MQ=255)
                                                                                               tGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTg                                            <  2:350689/140‑1 (MQ=255)
                                                                                               tGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTg                                            <  2:1706537/140‑1 (MQ=255)
                                                                                                ggtggCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTgg                                           <  2:692068/137‑1 (MQ=255)
                                                                                                     cAGCGCGATGAGAAATAATGATAATCCCCGCAATGTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCGGGGAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATa                                      <  2:549742/140‑1 (MQ=255)
                                                                                                       gcgcGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACa                                    <  2:1207098/140‑1 (MQ=255)
                                                                                                        cgtgATGAGAAATAATGATAATATCCGCATATTAACTAATCCTTCTGTCAGTCATGGGGAGCCTTTACATCTATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGAACGGCGACGGAGCTTGGGGATACaa                                   >  1:522225/4‑140 (MQ=255)
                                                                                                        cgtgATGAGAAATAATGATAATATCCGCATATTAACTAATCCTTCTGTCAGTCATGGGGAGCCTTTACATATATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGAACGGCGACGGAGCTTGGGGATACaa                                   >  1:772092/4‑140 (MQ=255)
                                                                                                        cgtgATGAGAAATAATGATAATATCCGCATATTAACTAATCCTTCTGTCAGTCATGGGGAGCCTTTACATATATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGAACGGCGACGGAGCTTGGGGATACaa                                   >  1:522254/4‑140 (MQ=255)
                                                                                                        cgcgATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACaa                                   >  2:495451/1‑140 (MQ=255)
                                                                                                                  aataatGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCat                         >  1:739162/1‑140 (MQ=255)
                                                                                                                         ataataCCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTg                  <  1:1679891/140‑1 (MQ=255)
                                                                                                                         ataataCCCGCAATTTAGCTAAGCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTg                  <  1:124091/140‑1 (MQ=255)
                                                                                                                           aataCCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTTTACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCa                >  2:32709/1‑140 (MQ=255)
                                                                                                                           aataCCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTGACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCATGGGGAGACAAAGAGACTCATTTATTTGCa                >  2:1358484/1‑140 (MQ=255)
                                                                                                                                 cGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGa          <  1:1652734/140‑1 (MQ=255)
                                                                                                                                  gCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGAt         >  1:647813/1‑140 (MQ=255)
                                                                                                                                   cAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATg        >  1:855820/1‑140 (MQ=255)
                                                                                                                                    aaTTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATgg       <  2:1749464/140‑1 (MQ=255)
                                                                                                                                      tttAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTTTACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaa     <  1:32709/140‑1 (MQ=255)
                                                                                                                                         aGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtg  <  1:1524826/140‑1 (MQ=255)
                                                                                                                                         aGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtg  <  1:2060813/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                          
CATGAGAAAACATTTCATCATGACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTG  >  NZ_CP034943/1761384‑1761660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: