Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,761,539 C→T 6.2% S16S (AGC→AGT EO946_RS09125 → non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,761,5390CT 6.2% 354.5 / 9.3 114S16S (AGC→AGTEO946_RS09125non‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base C (29/77);  new base T (5/2);  total (35/79)
Fisher's exact test for biased strand distribution p-value = 2.51e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01

Only 100 of 116 total aligned reads displayed.

ATCATGACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAA  >  NZ_CP034943/1761400‑1761671
                                                                                                                                           |                                                                                                                                    
atcatGACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCCGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGc                                                                                                                                      >  2:2428514/1‑140 (MQ=255)
     gACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATgg                                                                                                                                 >  1:1091138/1‑140 (MQ=255)
     gACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATgg                                                                                                                                 >  2:1531177/1‑140 (MQ=255)
     gACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATgg                                                                                                                                 >  2:256969/1‑140 (MQ=255)
       cGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTg                                                                                                                               <  2:839290/140‑1 (MQ=255)
       cGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTg                                                                                                                               <  1:1510667/140‑1 (MQ=255)
       cGAAGTCAAAACCAGAATCAAAGCGTTGTATGGAAATTAACTGAGAATCTACATAAAACTGCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTg                                                                                                                               <  1:1510800/140‑1 (MQ=255)
         aaGTCAAAACGAGAATCAAAGGTGTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTgag                                                                                                                             <  1:2402638/140‑1 (MQ=255)
          aGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTgaga                                                                                                                            >  2:502482/1‑140 (MQ=255)
          aGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTgaga                                                                                                                            >  2:2196134/1‑140 (MQ=255)
           gTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGAc                                                                                                                           >  2:1725502/1‑140 (MQ=255)
            tCAAAACGAGAATCAAAGGTTTTTATGGAAATTAACTGAGAATCTACATAAAAATTCGCATGTGAAATGGGGGCAGCGCGATGAGAAATAATGATACTACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                                          <  2:697174/140‑1 (MQ=255)
            tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                                          <  2:106018/140‑1 (MQ=255)
            tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                                          <  1:1175313/140‑1 (MQ=255)
            tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                                          <  1:369844/140‑1 (MQ=255)
            tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                                          <  1:389985/140‑1 (MQ=255)
            tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                                          <  1:745679/140‑1 (MQ=255)
            tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                                          <  2:2238289/140‑1 (MQ=255)
            tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                                          <  1:662600/140‑1 (MQ=255)
            tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                                          <  1:1547149/140‑1 (MQ=255)
            tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                                          <  1:151545/140‑1 (MQ=255)
              aaaaCGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACttt                                                                                                                        >  2:130301/1‑140 (MQ=255)
              aaaaCGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACttt                                                                                                                        >  2:614879/1‑140 (MQ=255)
               aaccGAGACTCACAGGCTTGTAGGGAAAGTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTa                                                                                                                       <  2:1977143/137‑1 (MQ=255)
                 aCGAGAATCAAAGGTTTGTATGGAAATTAACTGAGGATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACa                                                                                                                     <  1:1869727/140‑1 (MQ=255)
                 aCGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACa                                                                                                                     <  1:1722709/140‑1 (MQ=255)
                 aCGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACa                                                                                                                     <  2:1596459/140‑1 (MQ=255)
                 aCGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTACCTAATCCTTCTGTCAGCTATGGTGAGACTTTACa                                                                                                                     <  1:2032995/140‑1 (MQ=255)
                    agaATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACatat                                                                                                                  <  1:683719/140‑1 (MQ=255)
                      aaTCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACatatat                                                                                                                >  1:80605/1‑140 (MQ=255)
                      aaTCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACatatat                                                                                                                >  1:1749464/1‑140 (MQ=255)
                         cAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTg                                                                                                             <  1:2212998/140‑1 (MQ=255)
                         cAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTg                                                                                                             <  2:1439561/140‑1 (MQ=255)
                         cAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTg                                                                                                             <  1:719610/140‑1 (MQ=255)
                               tttGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGagc                                                                                                       <  2:1520443/140‑1 (MQ=255)
                               tttGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGagc                                                                                                       <  2:365083/140‑1 (MQ=255)
                               tttGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGagc                                                                                                       <  2:1002732/140‑1 (MQ=255)
                               tttGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGagc                                                                                                       <  2:1520481/140‑1 (MQ=255)
                                    aTGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGCTGAGAAATAATGATCAGACCCGCAATTTAGCTAATCCTACTGTCAGCTCTGGTGAGACTTTACATATATCTGAAGAGCAGCCg                                                                                                  <  1:2428514/140‑1 (MQ=255)
                                        aaaTTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCaa                                                                                              >  1:184220/1‑140 (MQ=255)
                                         aaTTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAATAGCAGCCGGCAAc                                                                                             >  2:2252314/1‑140 (MQ=255)
                                                 gcccaTCTACATAAACATTCCCAGGAGAAATGGGCGCAGCGCGATGAGAAATAATGATAATACCCGCACTTTAGCTACTCCTTCTGTCAGCTATGGTGAGACTTTACATATCTCTGAAGAGCAGCCGGCAACGATACCTg                                                                                     <  2:1254470/136‑1 (MQ=255)
                                                 gagaCTCTACATAAAACTTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAAGACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                                     <  2:1987399/140‑1 (MQ=255)
                                                 gagaATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                                     <  2:1347649/140‑1 (MQ=255)
                                                 gagaATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                                     <  1:1107516/140‑1 (MQ=255)
                                                 gagaATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                                     <  1:2196134/140‑1 (MQ=255)
                                                 gagaATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                                     <  1:502482/140‑1 (MQ=255)
                                                      tCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGtt                                                                                <  2:80605/140‑1 (MQ=255)
                                                           aTAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTtata                                                                           <  2:1679268/140‑1 (MQ=255)
                                                             aaaaaTTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACa                                                                         >  1:1207098/1‑140 (MQ=255)
                                                               aaaTTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCACCGATACCTGAAGTTTTATACAGa                                                                       >  2:1442300/1‑140 (MQ=255)
                                                               aaaTTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGa                                                                       >  2:505178/1‑140 (MQ=255)
                                                                attttgCAGGTGAAATGGAGGCAGCGTGATGAGAAATAATGATAATATCCGCATATTAACTAATCCTTCTGTCAGTCATGGGGAGCCTTTACATATATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGaa                                                                      >  1:2045715/6‑140 (MQ=255)
                                                                attttgCAGGTGAAATGGAGGCAGCGTGATGAGAAATAATGATAATATCCGCATATTAACTAATCCTTCTGTCAGTCATGGGGAGCCTTTACATATATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGaa                                                                      >  1:1995656/6‑140 (MQ=255)
                                                                        ggTGAAATGGGGGCAGCGCGATGAGAAATACTGATAATACCCGCAATTTAGCTAATCCTTCTGGCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTATTATACAGAACGGCTGCg                                                              <  2:145985/140‑1 (MQ=255)
                                                                        ggTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                              <  2:2431368/140‑1 (MQ=255)
                                                                        ggTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                              <  1:2083190/140‑1 (MQ=255)
                                                                        ggTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                              <  1:1120059/140‑1 (MQ=255)
                                                                        ggTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                              <  1:951635/140‑1 (MQ=255)
                                                                        ggTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                              <  2:438135/140‑1 (MQ=255)
                                                                        ggTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                              <  1:2206228/140‑1 (MQ=255)
                                                                        ggTGAAATGGAGGCAGCGTGATGAGAAATAATGATAATATCCGCATATTAACTAATCCTTCTGTCAGTCATGGGGAGCCTTTACATATATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGAACGGcgacg                                                              <  2:1995656/140‑5 (MQ=255)
                                                                        ggTGAAATGGAGGCAGCGTGATGAGAAATAATGATAATATCCGCATATTAACTAATCCGTCTGTCAGTCATGGGGAGCCTTTACATATATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGAACGGcgacg                                                              <  2:2045715/140‑5 (MQ=255)
                                                                             aaTGGGGCCAGCGCGATTAGACATAATGATAATACCCGCAATATAGATAATCCTTCAGTCAGATAGGGTGAGACTTTACAAATATCTGAAGATCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGACCt                                                         >  2:578821/1‑140 (MQ=255)
                                                                               tGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTg                                                       <  2:350689/140‑1 (MQ=255)
                                                                               tGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTg                                                       <  2:1706537/140‑1 (MQ=255)
                                                                                gggggCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAAGCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTgg                                                      <  1:1278560/140‑1 (MQ=255)
                                                                                     cAGCGCGATGAGAAATAATGATAATCCCCGCAATGTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCGGGGAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATa                                                 <  2:549742/140‑1 (MQ=255)
                                                                                       gcgcGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACa                                               <  2:1207098/140‑1 (MQ=255)
                                                                                        cgtgATGAGAAATAATGATAATATCCGCATATTAACTAATCCTTCTGTCAGTCATGGGGAGCCTTTACATCTATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGAACGGCGACGGAGCTTGGGGATACaa                                              >  1:522225/4‑140 (MQ=255)
                                                                                        cgtgATGAGAAATAATGATAATATCCGCATATTAACTAATCCTTCTGTCAGTCATGGGGAGCCTTTACATATATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGAACGGCGACGGAGCTTGGGGATACaa                                              >  1:772092/4‑140 (MQ=255)
                                                                                        cgtgATGAGAAATAATGATAATATCCGCATATTAACTAATCCTTCTGTCAGTCATGGGGAGCCTTTACATATATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGAACGGCGACGGAGCTTGGGGATACaa                                              >  1:522254/4‑140 (MQ=255)
                                                                                        cgcgATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACaa                                              >  2:495451/1‑140 (MQ=255)
                                                                                                  aataatGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCat                                    >  1:739162/1‑140 (MQ=255)
                                                                                                         ataataCCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTg                             <  1:1679891/140‑1 (MQ=255)
                                                                                                         ataataCCCGCAATTTAGCTAAGCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTg                             <  1:124091/140‑1 (MQ=255)
                                                                                                           aataCCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTTTACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCa                           >  2:32709/1‑140 (MQ=255)
                                                                                                           aataCCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTGACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCATGGGGAGACAAAGAGACTCATTTATTTGCa                           >  2:1358484/1‑140 (MQ=255)
                                                                                                                 cGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGa                     <  1:1652734/140‑1 (MQ=255)
                                                                                                                  gCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGag                    >  1:947653/1‑139 (MQ=255)
                                                                                                                  gCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGAt                    >  1:647813/1‑140 (MQ=255)
                                                                                                                   cAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATg                   >  1:855820/1‑140 (MQ=255)
                                                                                                                    aaTTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATgg                  <  2:1749464/140‑1 (MQ=255)
                                                                                                                      tttAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTTTACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaa                <  1:32709/140‑1 (MQ=255)
                                                                                                                         aGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtg             <  1:1524826/140‑1 (MQ=255)
                                                                                                                         aGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtg             <  1:2060813/140‑1 (MQ=255)
                                                                                                                           cTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaattgaa           <  2:907218/140‑1 (MQ=255)
                                                                                                                           cTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtgaa           <  2:2092059/140‑1 (MQ=255)
                                                                                                                           cTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtgaa           <  2:184220/140‑1 (MQ=255)
                                                                                                                           cTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtgaa           <  1:1531177/140‑1 (MQ=255)
                                                                                                                           cTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtgaa           <  2:1171958/140‑1 (MQ=255)
                                                                                                                           cTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtgaa           <  2:87866/140‑1 (MQ=255)
                                                                                                                           cTAATCCTTCTGTCAGCTATGGTGAGACTTTACATAGATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtgaa           <  2:1980395/140‑1 (MQ=255)
                                                                                                                              aTCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                              >  2:2225669/1‑86 (MQ=255)
                                                                                                                              aTCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                              <  1:2225669/86‑1 (MQ=255)
                                                                                                                              aTCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGaagtgaagtg        >  1:1488368/1‑140 (MQ=255)
                                                                                                                                   tCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCa   <  1:1725502/140‑1 (MQ=255)
                                                                                                                                   tCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCa   <  2:1537202/140‑1 (MQ=255)
                                                                                                                                   tCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCa   <  2:316045/140‑1 (MQ=255)
                                                                                                                                    cTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCaa  >  2:814525/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                    
ATCATGACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAA  >  NZ_CP034943/1761400‑1761671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: