Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,761,540 T→C 6.1% Y17H (TAT→CAT)  EO946_RS09125 → non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,761,5400TC 6.1% 359.3 / 9.1 114Y17H (TAT→CAT) EO946_RS09125non‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base T (30/76);  new base C (5/2);  total (35/79)
Fisher's exact test for biased strand distribution p-value = 2.87e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

GACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAA  >  NZ_CP034943/1761405‑1761671
                                                                                                                                       |                                                                                                                                   
gACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATgg                                                                                                                                 >  2:256969/1‑140 (MQ=255)
gACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATgg                                                                                                                                 >  1:746080/1‑140 (MQ=255)
gACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATgg                                                                                                                                 >  1:1091138/1‑140 (MQ=255)
gACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATgg                                                                                                                                 >  2:1531177/1‑140 (MQ=255)
gACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATgg                                                                                                                                 >  1:1439561/1‑140 (MQ=255)
  cGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTg                                                                                                                               <  1:1510667/140‑1 (MQ=255)
  cGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTg                                                                                                                               <  1:643540/140‑1 (MQ=255)
  cGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTg                                                                                                                               <  2:839290/140‑1 (MQ=255)
  cGAAGTCAAAACCAGAATCAAAGCGTTGTATGGAAATTAACTGAGAATCTACATAAAACTGCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTg                                                                                                                               <  1:1510800/140‑1 (MQ=255)
    aaGTCAAAACGAGAATCAAAGGTGTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTgag                                                                                                                             <  1:2402638/140‑1 (MQ=255)
     aGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTgaga                                                                                                                            >  2:502482/1‑140 (MQ=255)
     aGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTgaga                                                                                                                            >  2:2196134/1‑140 (MQ=255)
      gTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGAc                                                                                                                           >  2:1725502/1‑140 (MQ=255)
       tCAAAACGAGAATCAAAGGTTTTTATGGAAATTAACTGAGAATCTACATAAAAATTCGCATGTGAAATGGGGGCAGCGCGATGAGAAATAATGATACTACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                                          <  2:697174/140‑1 (MQ=255)
       tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                                          <  1:745679/140‑1 (MQ=255)
       tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                                          <  1:662600/140‑1 (MQ=255)
       tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                                          <  2:106018/140‑1 (MQ=255)
       tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                                          <  1:389985/140‑1 (MQ=255)
       tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                                          <  1:369844/140‑1 (MQ=255)
       tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                                          <  1:1175313/140‑1 (MQ=255)
       tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                                          <  1:151545/140‑1 (MQ=255)
       tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                                          <  1:1547149/140‑1 (MQ=255)
         aaaaCGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACttt                                                                                                                        >  2:130301/1‑140 (MQ=255)
          aaccGAGACTCACAGGCTTGTAGGGAAAGTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTa                                                                                                                       <  2:1977143/137‑1 (MQ=255)
            aCGAGAATCAAAGGTTTGTATGGAAATTAACTGAGGATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACa                                                                                                                     <  1:1869727/140‑1 (MQ=255)
            aCGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACa                                                                                                                     <  1:1546630/140‑1 (MQ=255)
            aCGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACa                                                                                                                     <  1:1722709/140‑1 (MQ=255)
            aCGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACa                                                                                                                     <  2:1596459/140‑1 (MQ=255)
            aCGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTACCTAATCCTTCTGTCAGCTATGGTGAGACTTTACa                                                                                                                     <  1:2032995/140‑1 (MQ=255)
               agaATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACatat                                                                                                                  <  1:683719/140‑1 (MQ=255)
                 aaTCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACatatat                                                                                                                >  1:1749464/1‑140 (MQ=255)
                 aaTCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACatatat                                                                                                                >  1:80605/1‑140 (MQ=255)
                    cAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTg                                                                                                             <  1:719610/140‑1 (MQ=255)
                    cAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTg                                                                                                             <  2:1439561/140‑1 (MQ=255)
                          tttGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGagc                                                                                                       <  2:1520443/140‑1 (MQ=255)
                          tttGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGagc                                                                                                       <  2:1520481/140‑1 (MQ=255)
                               aTGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGCTGAGAAATAATGATCAGACCCGCAATTTAGCTAATCCTACTGTCAGCTCTGGTGAGACTTTACATATATCTGAAGAGCAGCCg                                                                                                  <  1:2428514/140‑1 (MQ=255)
                               aTGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCg                                                                                                  <  1:1763586/140‑1 (MQ=255)
                                   aaaTTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCaa                                                                                              >  1:184220/1‑140 (MQ=255)
                                    aaTTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAATAGCAGCCGGCAAc                                                                                             >  2:2252314/1‑140 (MQ=255)
                                            gcccaTCTACATAAACATTCCCAGGAGAAATGGGCGCAGCGCGATGAGAAATAATGATAATACCCGCACTTTAGCTACTCCTTCTGTCAGCTATGGTGAGACTTTACATATCTCTGAAGAGCAGCCGGCAACGATACCTg                                                                                     <  2:1254470/136‑1 (MQ=255)
                                            gagaCTCTACATAAAACTTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAAGACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                                     <  2:1987399/140‑1 (MQ=255)
                                            gagaATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                                     <  2:1347649/140‑1 (MQ=255)
                                            gagaATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                                     <  2:1531585/140‑1 (MQ=255)
                                            gagaATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                                     <  1:1107516/140‑1 (MQ=255)
                                            gagaATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                                     <  1:2196134/140‑1 (MQ=255)
                                                 tCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGtt                                                                                <  2:80605/140‑1 (MQ=255)
                                                    aCATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTta                                                                             >  2:2070218/1‑140 (MQ=255)
                                                    aCAGAAAACTTCGCAGGTGAAAGGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTGTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTta                                                                             >  2:461013/1‑140 (MQ=255)
                                                      aTAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTtata                                                                           <  2:1679268/140‑1 (MQ=255)
                                                        aaaaaTTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACa                                                                         >  1:1207098/1‑140 (MQ=255)
                                                          aaaTTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCACCGATACCTGAAGTTTTATACAGa                                                                       >  2:1442300/1‑140 (MQ=255)
                                                          aaaTTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGa                                                                       >  2:505178/1‑140 (MQ=255)
                                                           attttgCAGGTGAAATGGAGGCAGCGTGATGAGAAATAATGATAATATCCGCATATTAACTAATCCTTCTGTCAGTCATGGGGAGCCTTTACATATATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGaa                                                                      >  1:2045715/6‑140 (MQ=255)
                                                                   ggTGAAATGGGGGCAGCGCGATGAGAAATACTGATAATACCCGCAATTTAGCTAATCCTTCTGGCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTATTATACAGAACGGCTGCg                                                              <  2:145985/140‑1 (MQ=255)
                                                                   ggTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                              <  2:438135/140‑1 (MQ=255)
                                                                   ggTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                              <  2:2431368/140‑1 (MQ=255)
                                                                   ggTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                              <  1:1120059/140‑1 (MQ=255)
                                                                   ggTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                              <  1:2083190/140‑1 (MQ=255)
                                                                   ggTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                              <  1:2206228/140‑1 (MQ=255)
                                                                   ggTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                              <  1:951635/140‑1 (MQ=255)
                                                                   ggTGAAATGGAGGCAGCGTGATGAGAAATAATGATAATATCCGCATATTAACTAATCCTTCTGTCAGTCATGGGGAGCCTTTACATATATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGAACGGcgacg                                                              <  2:1995656/140‑5 (MQ=255)
                                                                   ggTGAAATGGAGGCAGCGTGATGAGAAATAATGATAATATCCGCATATTAACTAATCCGTCTGTCAGTCATGGGGAGCCTTTACATATATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGAACGGcgacg                                                              <  2:2045715/140‑5 (MQ=255)
                                                                        aaTGGGGCCAGCGCGATTAGACATAATGATAATACCCGCAATATAGATAATCCTTCAGTCAGATAGGGTGAGACTTTACAAATATCTGAAGATCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGACCt                                                         >  2:578821/1‑140 (MQ=255)
                                                                          tGGGGGCAGCGCGATGAGAAATACTGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTg                                                       <  2:253692/140‑1 (MQ=255)
                                                                          tGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTg                                                       <  2:350689/140‑1 (MQ=255)
                                                                          tGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTg                                                       <  2:1706537/140‑1 (MQ=255)
                                                                           ggtggCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTgg                                                      <  2:692068/137‑1 (MQ=255)
                                                                           gggggCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAAGCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTgg                                                      <  1:1278560/140‑1 (MQ=255)
                                                                                cAGCGCGATGAGAAATAATGATAATCCCCGCAATGTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCGGGGAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATa                                                 <  2:549742/140‑1 (MQ=255)
                                                                                  gcgcGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACa                                               <  2:1207098/140‑1 (MQ=255)
                                                                                   cgtgATGAGAAATAATGATAATATCCGCATATTAACTAATCCTTCTGTCAGTCATGGGGAGCCTTTACATCTATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGAACGGCGACGGAGCTTGGGGATACaa                                              >  1:522225/4‑140 (MQ=255)
                                                                                   cgtgATGAGAAATAATGATAATATCCGCATATTAACTAATCCTTCTGTCAGTCATGGGGAGCCTTTACATATATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGAACGGCGACGGAGCTTGGGGATACaa                                              >  1:772092/4‑140 (MQ=255)
                                                                                   cgtgATGAGAAATAATGATAATATCCGCATATTAACTAATCCTTCTGTCAGTCATGGGGAGCCTTTACATATATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGAACGGCGACGGAGCTTGGGGATACaa                                              >  1:522254/4‑140 (MQ=255)
                                                                                   cgcgATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACaa                                              >  2:495451/1‑140 (MQ=255)
                                                                                                    ataataCCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTg                             <  1:1679891/140‑1 (MQ=255)
                                                                                                    ataataCCCGCAATTTAGCTAAGCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTg                             <  1:124091/140‑1 (MQ=255)
                                                                                                      aataCCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTTTACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCa                           >  2:32709/1‑140 (MQ=255)
                                                                                                      aataCCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTGACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCATGGGGAGACAAAGAGACTCATTTATTTGCa                           >  2:1358484/1‑140 (MQ=255)
                                                                                                             gCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGag                    >  1:947653/1‑139 (MQ=255)
                                                                                                             gCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGAt                    >  1:647813/1‑140 (MQ=255)
                                                                                                              cAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATg                   >  1:855820/1‑140 (MQ=255)
                                                                                                               aaTTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATgg                  <  2:1749464/140‑1 (MQ=255)
                                                                                                                 tttAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTTTACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaa                <  1:32709/140‑1 (MQ=255)
                                                                                                                    aGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtg             <  1:1524826/140‑1 (MQ=255)
                                                                                                                    aGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtg             <  1:2060813/140‑1 (MQ=255)
                                                                                                                      cgaaTCCTTCTGTGAGCGATGGTGAGACTTTTCATATATCTGAAGATCATCCGGCATCGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtgaa           <  2:1034546/138‑1 (MQ=255)
                                                                                                                      cTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaattgaa           <  2:907218/140‑1 (MQ=255)
                                                                                                                      cTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtgaa           <  2:2092059/140‑1 (MQ=255)
                                                                                                                      cTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtgaa           <  2:184220/140‑1 (MQ=255)
                                                                                                                      cTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtgaa           <  2:1171958/140‑1 (MQ=255)
                                                                                                                      cTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtgaa           <  1:1531177/140‑1 (MQ=255)
                                                                                                                      cTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtgaa           <  2:87866/140‑1 (MQ=255)
                                                                                                                      cTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGAGACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtgaa           <  1:256969/140‑1 (MQ=255)
                                                                                                                         aTCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                              >  2:2225669/1‑86 (MQ=255)
                                                                                                                         aTCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                              <  1:2225669/86‑1 (MQ=255)
                                                                                                                              tCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCa   <  2:316045/140‑1 (MQ=255)
                                                                                                                              tCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCa   <  1:1725502/140‑1 (MQ=255)
                                                                                                                               cTGTCAGCTATTGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCaa  <  1:2070218/140‑1 (MQ=255)
                                                                                                                               cTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCaa  >  2:814525/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                   
GACGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAA  >  NZ_CP034943/1761405‑1761671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: