Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,761,545 T→G 6.2% G18G (GGT→GGG EO946_RS09125 → non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,761,5450TG 6.2% 347.7 / 8.3 113G18G (GGT→GGGEO946_RS09125non‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base T (25/81);  new base G (5/2);  total (30/83)
Fisher's exact test for biased strand distribution p-value = 1.39e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 115 total aligned reads displayed.

CGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGAT  >  NZ_CP034943/1761407‑1761684
                                                                                                                                          |                                                                                                                                           
cGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTg                                                                                                                                            <  1:643540/140‑1 (MQ=255)
cGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTg                                                                                                                                            <  2:839290/140‑1 (MQ=255)
cGAAGTCAAAACCAGAATCAAAGCGTTGTATGGAAATTAACTGAGAATCTACATAAAACTGCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTg                                                                                                                                            <  1:1510800/140‑1 (MQ=255)
  aaGTCAAAACGAGAATCAAAGGTGTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTgag                                                                                                                                          <  1:2402638/140‑1 (MQ=255)
   aGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTgaga                                                                                                                                         >  2:2196134/1‑140 (MQ=255)
   aGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTgaga                                                                                                                                         >  2:502482/1‑140 (MQ=255)
    gTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGAc                                                                                                                                        >  2:1725502/1‑140 (MQ=255)
     tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                                                       <  1:151545/140‑1 (MQ=255)
     tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                                                       <  1:369844/140‑1 (MQ=255)
     tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                                                       <  1:1175313/140‑1 (MQ=255)
     tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                                                       <  1:662600/140‑1 (MQ=255)
     tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                                                       <  1:745679/140‑1 (MQ=255)
     tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                                                       <  2:2238289/140‑1 (MQ=255)
     tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                                                       <  1:1547149/140‑1 (MQ=255)
     tCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACt                                                                                                                                       <  2:106018/140‑1 (MQ=255)
       aaaaCGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACttt                                                                                                                                     >  2:614879/1‑140 (MQ=255)
        aaccGAGACTCACAGGCTTGTAGGGAAAGTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTa                                                                                                                                    <  2:1977143/137‑1 (MQ=255)
          aCGAGAATCAAAGGTTTGTATGGAAATTAACTGAGGATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACa                                                                                                                                  <  1:1869727/140‑1 (MQ=255)
          aCGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACa                                                                                                                                  <  1:1722709/140‑1 (MQ=255)
          aCGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACa                                                                                                                                  <  2:1596459/140‑1 (MQ=255)
          aCGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACa                                                                                                                                  <  1:1546630/140‑1 (MQ=255)
          aCGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTACCTAATCCTTCTGTCAGCTATGGTGAGACTTTACa                                                                                                                                  <  1:2032995/140‑1 (MQ=255)
               aaTCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACatatat                                                                                                                             >  1:80605/1‑140 (MQ=255)
               aaTCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACatatat                                                                                                                             >  1:1749464/1‑140 (MQ=255)
                  cAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTg                                                                                                                          <  2:1439561/140‑1 (MQ=255)
                  cAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTg                                                                                                                          <  1:719610/140‑1 (MQ=255)
                        tttGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGagc                                                                                                                    <  2:365083/140‑1 (MQ=255)
                        tttGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGagc                                                                                                                    <  2:1002732/140‑1 (MQ=255)
                        tttGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGagc                                                                                                                    <  2:1520481/140‑1 (MQ=255)
                             aTGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGCTGAGAAATAATGATCAGACCCGCAATTTAGCTAATCCTACTGTCAGCTCTGGTGAGACTTTACATATATCTGAAGAGCAGCCg                                                                                                               <  1:2428514/140‑1 (MQ=255)
                             aTGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCg                                                                                                               <  1:1763586/140‑1 (MQ=255)
                                 aaaTTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCaa                                                                                                           >  1:184220/1‑140 (MQ=255)
                                  aaTTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAATAGCAGCCGGCAAc                                                                                                          >  2:2252314/1‑140 (MQ=255)
                                          gcccaTCTACATAAACATTCCCAGGAGAAATGGGCGCAGCGCGATGAGAAATAATGATAATACCCGCACTTTAGCTACTCCTTCTGTCAGCTATGGTGAGACTTTACATATCTCTGAAGAGCAGCCGGCAACGATACCTg                                                                                                  <  2:1254470/136‑1 (MQ=255)
                                          gagaCTCTACATAAAACTTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAAGACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                                                  <  2:1987399/140‑1 (MQ=255)
                                          gagaATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                                                  <  2:1406847/140‑1 (MQ=255)
                                          gagaATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                                                  <  2:1531585/140‑1 (MQ=255)
                                          gagaATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                                                  <  2:1347649/140‑1 (MQ=255)
                                          gagaATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                                                  <  1:502482/140‑1 (MQ=255)
                                          gagaATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                                                  <  1:1107516/140‑1 (MQ=255)
                                          gagaATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                                                  <  1:2196134/140‑1 (MQ=255)
                                               tCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGtt                                                                                             <  2:80605/140‑1 (MQ=255)
                                                  aCATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTta                                                                                          >  2:2070218/1‑140 (MQ=255)
                                                  aCAGAAAACTTCGCAGGTGAAAGGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTGTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTta                                                                                          >  2:461013/1‑140 (MQ=255)
                                                    aTAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTtata                                                                                        <  2:1679268/140‑1 (MQ=255)
                                                      aaaaaTTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACa                                                                                      >  1:1207098/1‑140 (MQ=255)
                                                        aaaTTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCACCGATACCTGAAGTTTTATACAGa                                                                                    >  2:1442300/1‑140 (MQ=255)
                                                        aaaTTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGa                                                                                    >  2:505178/1‑140 (MQ=255)
                                                         attttgCAGGTGAAATGGAGGCAGCGTGATGAGAAATAATGATAATATCCGCATATTAACTAATCCTTCTGTCAGTCATGGGGAGCCTTTACATATATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGaa                                                                                   >  1:2045715/6‑140 (MQ=255)
                                                                 ggTGAAATGGGGGCAGCGCGATGAGAAATACTGATAATACCCGCAATTTAGCTAATCCTTCTGGCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTATTATACAGAACGGCTGCg                                                                           <  2:145985/140‑1 (MQ=255)
                                                                 ggTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                                           <  1:1120059/140‑1 (MQ=255)
                                                                 ggTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                                           <  2:2431368/140‑1 (MQ=255)
                                                                 ggTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                                           <  1:2206228/140‑1 (MQ=255)
                                                                 ggTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                                           <  2:438135/140‑1 (MQ=255)
                                                                 ggTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                                           <  1:2083190/140‑1 (MQ=255)
                                                                 ggTGAAATGGAGGCAGCGTGATGAGAAATAATGATAATATCCGCATATTAACTAATCCTTCTGTCAGTCATGGGGAGCCTTTACATATATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGAACGGcgacg                                                                           <  2:1995656/140‑5 (MQ=255)
                                                                 ggTGAAATGGAGGCAGCGTGATGAGAAATAATGATAATATCCGCATATTAACTAATCCGTCTGTCAGTCATGGGGAGCCTTTACATATATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGAACGGcgacg                                                                           <  2:2045715/140‑5 (MQ=255)
                                                                      aaTGGGGCCAGCGCGATTAGACATAATGATAATACCCGCAATATAGATAATCCTTCAGTCAGATAGGGTGAGACTTTACAAATATCTGAAGATCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGACCt                                                                      >  2:578821/1‑140 (MQ=255)
                                                                        tGGGGGCAGCGCGATGAGAAATACTGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTg                                                                    <  2:253692/140‑1 (MQ=255)
                                                                        tGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTg                                                                    <  2:350689/140‑1 (MQ=255)
                                                                        tGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTg                                                                    <  2:1706537/140‑1 (MQ=255)
                                                                         ggtggCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTgg                                                                   <  2:692068/137‑1 (MQ=255)
                                                                         gggggCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAAGCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTgg                                                                   <  1:1278560/140‑1 (MQ=255)
                                                                              cAGCGCGATGAGAAATAATGATAATCCCCGCAATGTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCGGGGAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATa                                                              <  2:549742/140‑1 (MQ=255)
                                                                                gcgcGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACa                                                            <  2:1207098/140‑1 (MQ=255)
                                                                                 cgtgATGAGAAATAATGATAATATCCGCATATTAACTAATCCTTCTGTCAGTCATGGGGAGCCTTTACATCTATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGAACGGCGACGGAGCTTGGGGATACaa                                                           >  1:522225/4‑140 (MQ=255)
                                                                                 cgtgATGAGAAATAATGATAATATCCGCATATTAACTAATCCTTCTGTCAGTCATGGGGAGCCTTTACATATATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGAACGGCGACGGAGCTTGGGGATACaa                                                           >  1:772092/4‑140 (MQ=255)
                                                                                 cgtgATGAGAAATAATGATAATATCCGCATATTAACTAATCCTTCTGTCAGTCATGGGGAGCCTTTACATATATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGAACGGCGACGGAGCTTGGGGATACaa                                                           >  1:522254/4‑140 (MQ=255)
                                                                                 cgcgATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACaa                                                           >  2:495451/1‑140 (MQ=255)
                                                                                           aataatGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCat                                                 >  1:739162/1‑140 (MQ=255)
                                                                                                  ataataCCCGCAATTTAGCTAAGCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTg                                          <  1:124091/140‑1 (MQ=255)
                                                                                                    aataCCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTTTACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCa                                        >  2:32709/1‑140 (MQ=255)
                                                                                                    aataCCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTGACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCATGGGGAGACAAAGAGACTCATTTATTTGCa                                        >  2:1358484/1‑140 (MQ=255)
                                                                                                          cGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGa                                  <  1:1652734/140‑1 (MQ=255)
                                                                                                           gCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGag                                 >  1:947653/1‑139 (MQ=255)
                                                                                                           gCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGAt                                 >  1:647813/1‑140 (MQ=255)
                                                                                                            cAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATg                                >  1:855820/1‑140 (MQ=255)
                                                                                                               tttAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTTTACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaa                             <  1:32709/140‑1 (MQ=255)
                                                                                                                  aGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtg                          <  1:2060813/140‑1 (MQ=255)
                                                                                                                  aGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtg                          <  1:1524826/140‑1 (MQ=255)
                                                                                                                    cgaaTCCTTCTGTGAGCGATGGTGAGACTTTTCATATATCTGAAGATCATCCGGCATCGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtgaa                        <  2:1034546/138‑1 (MQ=255)
                                                                                                                    cTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaattgaa                        <  2:907218/140‑1 (MQ=255)
                                                                                                                    cTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtgaa                        <  2:184220/140‑1 (MQ=255)
                                                                                                                    cTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtgaa                        <  2:87866/140‑1 (MQ=255)
                                                                                                                    cTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtgaa                        <  2:1171958/140‑1 (MQ=255)
                                                                                                                    cTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtgaa                        <  1:1531177/140‑1 (MQ=255)
                                                                                                                    cTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGAGACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtgaa                        <  1:256969/140‑1 (MQ=255)
                                                                                                                    cTAATCCTTCTGTCAGCTATGGTGAGACTTTACATAGATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtgaa                        <  2:1980395/140‑1 (MQ=255)
                                                                                                                       aTCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                                           >  2:2225669/1‑86 (MQ=255)
                                                                                                                       aTCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                                           <  1:2225669/86‑1 (MQ=255)
                                                                                                                       aTCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGaagtgaagtg                     >  1:1488368/1‑140 (MQ=255)
                                                                                                                            tCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCa                <  1:1725502/140‑1 (MQ=255)
                                                                                                                            tCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCa                <  2:1537202/140‑1 (MQ=255)
                                                                                                                             cTGTCAGCTATTGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCaa               <  1:2070218/140‑1 (MQ=255)
                                                                                                                             cTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCaa               >  2:814525/1‑140 (MQ=255)
                                                                                                                                      aTGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGa      <  1:2360096/140‑1 (MQ=255)
                                                                                                                                      aTGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGa      <  2:558087/140‑1 (MQ=255)
                                                                                                                                      aTGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGa      <  2:746080/140‑1 (MQ=255)
                                                                                                                                      aTGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGa      <  2:1091138/140‑1 (MQ=255)
                                                                                                                                          tGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTAACAATCATATGGACGAt  >  2:471732/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                           
CGAAGTCAAAACGAGAATCAAAGGTTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGAT  >  NZ_CP034943/1761407‑1761684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: