Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,761,567 G→A 8.7% E26K (GAG→AAG)  EO946_RS09125 → non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,761,5670GA 8.7% 346.2 / 16.0 114E26K (GAG→AAG) EO946_RS09125non‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base G (40/63);  new base A (8/2);  total (49/65)
Fisher's exact test for biased strand distribution p-value = 1.74e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

TTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAATGATGCGTTGCGTAT  >  NZ_CP034943/1761431‑1761706
                                                                                                                                        |                                                                                                                                           
tttGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGagc                                                                                                                                          <  2:1520481/140‑1 (MQ=255)
tttGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGagc                                                                                                                                          <  2:1002732/140‑1 (MQ=255)
tttGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGagc                                                                                                                                          <  2:1520443/140‑1 (MQ=255)
tttGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGagc                                                                                                                                          <  2:365083/140‑1 (MQ=255)
     aTGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGCTGAGAAATAATGATCAGACCCGCAATTTAGCTAATCCTACTGTCAGCTCTGGTGAGACTTTACATATATCTGAAGAGCAGCCg                                                                                                                                     <  1:2428514/140‑1 (MQ=255)
     aTGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCg                                                                                                                                     <  1:1763586/140‑1 (MQ=255)
         aaaTTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCaa                                                                                                                                 >  1:184220/1‑140 (MQ=255)
          aaTTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAATAGCAGCCGGCAAc                                                                                                                                >  2:2252314/1‑140 (MQ=255)
                  gcccaTCTACATAAACATTCCCAGGAGAAATGGGCGCAGCGCGATGAGAAATAATGATAATACCCGCACTTTAGCTACTCCTTCTGTCAGCTATGGTGAGACTTTACATATCTCTGAAGAGCAGCCGGCAACGATACCTg                                                                                                                        <  2:1254470/136‑1 (MQ=255)
                  gagaCTCTACATAAAACTTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAAGACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                                                                        <  2:1987399/140‑1 (MQ=255)
                  gagaATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                                                                        <  2:1406847/140‑1 (MQ=255)
                  gagaATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                                                                        <  1:502482/140‑1 (MQ=255)
                  gagaATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                                                                        <  1:1107516/140‑1 (MQ=255)
                  gagaATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                                                                        <  1:2196134/140‑1 (MQ=255)
                  gagaATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                                                                        <  2:1347649/140‑1 (MQ=255)
                       tCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGtt                                                                                                                   <  2:80605/140‑1 (MQ=255)
                          aCAGAAAACTTCGCAGGTGAAAGGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTGTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTta                                                                                                                >  2:461013/1‑140 (MQ=255)
                            aTAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTtata                                                                                                              <  2:1679268/140‑1 (MQ=255)
                              aaaaaTTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACa                                                                                                            >  1:1207098/1‑140 (MQ=255)
                                aaaTTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCACCGATACCTGAAGTTTTATACAGa                                                                                                          >  2:1442300/1‑140 (MQ=255)
                                aaaTTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGa                                                                                                          >  2:505178/1‑140 (MQ=255)
                                 attttgCAGGTGAAATGGAGGCAGCGTGATGAGAAATAATGATAATATCCGCATATTAACTAATCCTTCTGTCAGTCATGGGGAGCCTTTACATATATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGaa                                                                                                         >  1:1995656/6‑140 (MQ=255)
                                 attttgCAGGTGAAATGGAGGCAGCGTGATGAGAAATAATGATAATATCCGCATATTAACTAATCCTTCTGTCAGTCATGGGGAGCCTTTACATATATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGaa                                                                                                         >  1:2045715/6‑140 (MQ=255)
                                         ggTGAAATGGGGGCAGCGCGATGAGAAATACTGATAATACCCGCAATTTAGCTAATCCTTCTGGCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTATTATACAGAACGGCTGCg                                                                                                 <  2:145985/140‑1 (MQ=255)
                                         ggTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                                                                 <  1:951635/140‑1 (MQ=255)
                                         ggTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                                                                 <  1:1120059/140‑1 (MQ=255)
                                         ggTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                                                                 <  1:2206228/140‑1 (MQ=255)
                                         ggTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                                                                 <  1:2083190/140‑1 (MQ=255)
                                         ggTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                                                                 <  2:2431368/140‑1 (MQ=255)
                                         ggTGAAATGGAGGCAGCGTGATGAGAAATAATGATAATATCCGCATATTAACTAATCCTTCTGTCAGTCATGGGGAGCCTTTACATATATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGAACGGcgacg                                                                                                 <  2:1995656/140‑5 (MQ=255)
                                         ggTGAAATGGAGGCAGCGTGATGAGAAATAATGATAATATCCGCATATTAACTAATCCGTCTGTCAGTCATGGGGAGCCTTTACATATATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGAACGGcgacg                                                                                                 <  2:2045715/140‑5 (MQ=255)
                                              aaTGGGGCCAGCGCGATTAGACATAATGATAATACCCGCAATATAGATAATCCTTCAGTCAGATAGGGTGAGACTTTACAAATATCTGAAGATCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGACCt                                                                                            >  2:578821/1‑140 (MQ=255)
                                                tGGGGGCAGCGCGATGAGAAATACTGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTg                                                                                          <  2:253692/140‑1 (MQ=255)
                                                tGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTg                                                                                          <  2:1706537/140‑1 (MQ=255)
                                                tGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTg                                                                                          <  2:928270/140‑1 (MQ=255)
                                                tGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTg                                                                                          <  2:350689/140‑1 (MQ=255)
                                                 ggtggCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTgg                                                                                         <  2:692068/137‑1 (MQ=255)
                                                 gggggCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAAGCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTgg                                                                                         <  1:1278560/140‑1 (MQ=255)
                                                        gcgcGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACa                                                                                  <  2:1207098/140‑1 (MQ=255)
                                                         cgtgATGAGAAATAATGATAATATCCGCATATTAACTAATCCTTCTGTCAGTCATGGGGAGCCTTTACATCTATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGAACGGCGACGGAGCTTGGGGATACaa                                                                                 >  1:522225/4‑140 (MQ=255)
                                                         cgtgATGAGAAATAATGATAATATCCGCATATTAACTAATCCTTCTGTCAGTCATGGGGAGCCTTTACATATATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGAACGGCGACGGAGCTTGGGGATACaa                                                                                 >  1:772092/4‑140 (MQ=255)
                                                         cgtgATGAGAAATAATGATAATATCCGCATATTAACTAATCCTTCTGTCAGTCATGGGGAGCCTTTACATATATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGAACGGCGACGGAGCTTGGGGATACaa                                                                                 >  1:522254/4‑140 (MQ=255)
                                                         cgcgATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACaa                                                                                 >  2:495451/1‑140 (MQ=255)
                                                                   aataatGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCat                                                                       >  1:739162/1‑140 (MQ=255)
                                                                          ataataCCCGCAATTTAGCTAAGCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTg                                                                <  1:124091/140‑1 (MQ=255)
                                                                            aataCCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTTTACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCa                                                              >  2:32709/1‑140 (MQ=255)
                                                                            aataCCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTGACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCATGGGGAGACAAAGAGACTCATTTATTTGCa                                                              >  2:1358484/1‑140 (MQ=255)
                                                                                  cGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGa                                                        <  1:1652734/140‑1 (MQ=255)
                                                                                   gCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGag                                                       >  1:947653/1‑139 (MQ=255)
                                                                                   gCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGAt                                                       >  1:647813/1‑140 (MQ=255)
                                                                                    cAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATg                                                      >  1:855820/1‑140 (MQ=255)
                                                                                     aaTTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATgg                                                     <  2:1749464/140‑1 (MQ=255)
                                                                                       tttAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTTTACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaa                                                   <  1:32709/140‑1 (MQ=255)
                                                                                          aGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtg                                                <  1:1524826/140‑1 (MQ=255)
                                                                                          aGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtg                                                <  1:2060813/140‑1 (MQ=255)
                                                                                            cTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaattgaa                                              <  2:907218/140‑1 (MQ=255)
                                                                                            cTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtgaa                                              <  2:1171958/140‑1 (MQ=255)
                                                                                            cTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtgaa                                              <  2:2092059/140‑1 (MQ=255)
                                                                                            cTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtgaa                                              <  2:87866/140‑1 (MQ=255)
                                                                                            cTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtgaa                                              <  2:184220/140‑1 (MQ=255)
                                                                                            cTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtgaa                                              <  1:1531177/140‑1 (MQ=255)
                                                                                            cTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGAGACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtgaa                                              <  1:256969/140‑1 (MQ=255)
                                                                                            cTAATCCTTCTGTCAGCTATGGTGAGACTTTACATAGATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtgaa                                              <  2:1980395/140‑1 (MQ=255)
                                                                                               aTCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                                                                 >  2:2225669/1‑86 (MQ=255)
                                                                                               aTCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGaagtgaagtg                                           >  1:1488368/1‑140 (MQ=255)
                                                                                                    tCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCa                                      <  2:316045/140‑1 (MQ=255)
                                                                                                    tCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCa                                      <  2:1537202/140‑1 (MQ=255)
                                                                                                    tCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCa                                      <  1:1725502/140‑1 (MQ=255)
                                                                                                     cTGTCAGCTATTGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCaa                                     <  1:2070218/140‑1 (MQ=255)
                                                                                                              aTGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGa                            <  1:2360096/140‑1 (MQ=255)
                                                                                                              aTGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGa                            <  2:558087/140‑1 (MQ=255)
                                                                                                              aTGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGa                            <  2:746080/140‑1 (MQ=255)
                                                                                                              aTGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGa                            <  2:1091138/140‑1 (MQ=255)
                                                                                                                  tGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGAt                        >  2:49959/1‑140 (MQ=255)
                                                                                                                  tGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTAACAATCATATGGACGAt                        >  2:471732/1‑140 (MQ=255)
                                                                                                                    agaCTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGAt                                                                                     <  1:2375338/77‑1 (MQ=255)
                                                                                                                      aCTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaa                                                   <  2:430327/109‑1 (MQ=255)
                                                                                                                      aCTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATtgtg                    >  1:1237803/1‑140 (MQ=255)
                                                                                                                      aCTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATtgtg                    <  1:293862/140‑1 (MQ=255)
                                                                                                                      aCTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATtgtg                    <  1:78543/140‑1 (MQ=255)
                                                                                                                      aCTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATtgtg                    <  1:1147168/140‑1 (MQ=255)
                                                                                                                      aCTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATtgtg                    >  1:1595846/1‑140 (MQ=255)
                                                                                                                          tACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAat                >  1:1734241/1‑140 (MQ=255)
                                                                                                                             atatatCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAatgat             <  2:983916/140‑1 (MQ=255)
                                                                                                                                tatCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGAACGGCGACGGAGCTTGGGGATACAAAGGGACTCATTTATTTGCAGCCGGCTGGAACGGAAGTTTACCAATCATACAGACGAGTCTGGGatgatgga          >  2:2024113/1‑138 (MQ=255)
                                                                                                                                tatCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGAACGGCGACGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAACTGAAGTTTACCAATCATACAGACGATTATGGGatgatgga          >  2:2285166/1‑138 (MQ=255)
                                                                                                                                tatCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGAACGGCGACGGAGCTTGGGGATACAAAGGGAATAATTTATTTGCAGCCGGATGGAACTGAAGTTTACCAATCATACAGACGATTATGGGatgatgga          >  2:1430205/1‑138 (MQ=255)
                                                                                                                                 atCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAATGAtgcgt         >  1:2229066/1‑140 (MQ=255)
                                                                                                                                 atCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAATGAtgcgt         >  2:1873911/1‑140 (MQ=255)
                                                                                                                                 atCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAATGAtgcgt         >  2:934474/1‑140 (MQ=255)
                                                                                                                                  tCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAATGAtgcgtt        >  1:1384007/1‑140 (MQ=255)
                                                                                                                                  tCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAATGAtgcgtt        >  2:863010/1‑140 (MQ=255)
                                                                                                                                    tGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAATGAtgcgttgc      >  2:2124095/1‑140 (MQ=255)
                                                                                                                                    tGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAATGAtgcgttgc      >  2:2056373/1‑140 (MQ=255)
                                                                                                                                    tGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAATGAtgcgttgc      >  2:1165813/1‑140 (MQ=255)
                                                                                                                                      aaGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAATGAtgcgttgcgt    >  2:445848/1‑140 (MQ=255)
                                                                                                                                      aaGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAATGAtgcgttgcgt    >  1:1724232/1‑140 (MQ=255)
                                                                                                                                      aaGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAATGAtgcgttgcgt    >  2:1008532/1‑140 (MQ=255)
                                                                                                                                        gagCCGCCGGCAACGATACCTGAAGTTTTATACAGACCGGCTGCGGAGCTTGGGGATACACAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTCCCAATCATATGGACGATTGTGGAATGATGCGTTGCGTAt  <  2:682558/140‑1 (MQ=255)
                                                                                                                                        gagCAGCCTGCAACGATACCTGACGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGGCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAATGATGCGTTGCGTAt  <  2:211867/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                           
TTTGTATGGAAATTAACTGAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAATGATGCGTTGCGTAT  >  NZ_CP034943/1761431‑1761706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: