Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,761,581 G→A 8.0% T30T (ACG→ACA EO946_RS09125 → non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,761,5810GA 8.0% 383.2 / 16.9 124T30T (ACG→ACAEO946_RS09125non‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base G (51/63);  new base A (8/2);  total (59/65)
Fisher's exact test for biased strand distribution p-value = 4.63e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01

Only 100 of 124 total aligned reads displayed.

GAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAATGATGCGTTGCGTATCGTGAAGG  >  NZ_CP034943/1761449‑1761714
                                                                                                                                    |                                                                                                                                     
gcccaTCTACATAAACATTCCCAGGAGAAATGGGCGCAGCGCGATGAGAAATAATGATAATACCCGCACTTTAGCTACTCCTTCTGTCAGCTATGGTGAGACTTTACATATCTCTGAAGAGCAGCCGGCAACGATACCTg                                                                                                                                <  2:1254470/136‑1 (MQ=255)
gagaCTCTACATAAAACTTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAAGACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                                                                                <  2:1987399/140‑1 (MQ=255)
gagaATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                                                                                <  2:1347649/140‑1 (MQ=255)
gagaATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                                                                                <  2:1406847/140‑1 (MQ=255)
gagaATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                                                                                <  1:502482/140‑1 (MQ=255)
gagaATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTg                                                                                                                                <  1:1107516/140‑1 (MQ=255)
     tCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGtt                                                                                                                           <  2:80605/140‑1 (MQ=255)
          aTAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTtata                                                                                                                      <  2:1679268/140‑1 (MQ=255)
            aaaaaTTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACa                                                                                                                    >  1:1207098/1‑140 (MQ=255)
              aaaTTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCACCGATACCTGAAGTTTTATACAGa                                                                                                                  >  2:1442300/1‑140 (MQ=255)
              aaaTTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGa                                                                                                                  >  2:505178/1‑140 (MQ=255)
               attttgCAGGTGAAATGGAGGCAGCGTGATGAGAAATAATGATAATATCCGCATATTAACTAATCCTTCTGTCAGTCATGGGGAGCCTTTACATATATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGaa                                                                                                                 >  1:1995656/6‑140 (MQ=255)
                       ggTGAAATGGGGGCAGCGCGATGAGAAATACTGATAATACCCGCAATTTAGCTAATCCTTCTGGCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTATTATACAGAACGGCTGCg                                                                                                         <  2:145985/140‑1 (MQ=255)
                       ggTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                                                                         <  1:1120059/140‑1 (MQ=255)
                       ggTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                                                                         <  1:2206228/140‑1 (MQ=255)
                       ggTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                                                                         <  1:2083190/140‑1 (MQ=255)
                       ggTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                                                                         <  2:2431368/140‑1 (MQ=255)
                       ggTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                                                                         <  2:438135/140‑1 (MQ=255)
                       ggTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                                                                         <  1:951635/140‑1 (MQ=255)
                       ggTGAAATGGAGGCAGCGTGATGAGAAATAATGATAATATCCGCATATTAACTAATCCGTCTGTCAGTCATGGGGAGCCTTTACATATATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGAACGGcgacg                                                                                                         <  2:2045715/140‑5 (MQ=255)
                            aaTGGGGCCAGCGCGATTAGACATAATGATAATACCCGCAATATAGATAATCCTTCAGTCAGATAGGGTGAGACTTTACAAATATCTGAAGATCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGACCt                                                                                                    >  2:578821/1‑140 (MQ=255)
                              tGGGGGCAGCGCGATGAGAAATACTGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTg                                                                                                  <  2:253692/140‑1 (MQ=255)
                              tGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTg                                                                                                  <  2:350689/140‑1 (MQ=255)
                              tGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTg                                                                                                  <  2:1706537/140‑1 (MQ=255)
                              tGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTg                                                                                                  <  2:928270/140‑1 (MQ=255)
                               ggtggCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTgg                                                                                                 <  2:692068/137‑1 (MQ=255)
                               gggggCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAAGCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTgg                                                                                                 <  1:1278560/140‑1 (MQ=255)
                                      gcgcGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACa                                                                                          <  2:1207098/140‑1 (MQ=255)
                                       cgtgATGAGAAATAATGATAATATCCGCATATTAACTAATCCTTCTGTCAGTCATGGGGAGCCTTTACATCTATCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGAACGGCGACGGAGCTTGGGGATACaa                                                                                         >  1:522225/4‑140 (MQ=255)
                                       cgcgATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACaa                                                                                         >  2:495451/1‑140 (MQ=255)
                                                 aataatGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCat                                                                               >  1:739162/1‑140 (MQ=255)
                                                        ataataCCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTg                                                                        <  1:1679891/140‑1 (MQ=255)
                                                                 gCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGAt                                                               >  1:647813/1‑140 (MQ=255)
                                                                  cAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATg                                                              >  1:855820/1‑140 (MQ=255)
                                                                   aaTTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATgg                                                             <  2:1749464/140‑1 (MQ=255)
                                                                     tttAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTTTACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaa                                                           <  1:32709/140‑1 (MQ=255)
                                                                        aGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtg                                                        <  1:2060813/140‑1 (MQ=255)
                                                                        aGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtg                                                        <  1:1524826/140‑1 (MQ=255)
                                                                          cgaaTCCTTCTGTGAGCGATGGTGAGACTTTTCATATATCTGAAGATCATCCGGCATCGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtgaa                                                      <  2:1034546/138‑1 (MQ=255)
                                                                          cTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtgaa                                                      <  2:2092059/140‑1 (MQ=255)
                                                                          cTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtgaa                                                      <  2:87866/140‑1 (MQ=255)
                                                                          cTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtgaa                                                      <  2:1171958/140‑1 (MQ=255)
                                                                          cTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtgaa                                                      <  1:1531177/140‑1 (MQ=255)
                                                                          cTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGAGACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtgaa                                                      <  1:256969/140‑1 (MQ=255)
                                                                          cTAATCCTTCTGTCAGCTATGGTGAGACTTTACATAGATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaagtgaa                                                      <  2:1980395/140‑1 (MQ=255)
                                                                             aTCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                                                                         >  2:2225669/1‑86 (MQ=255)
                                                                             aTCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCg                                                                                                         <  1:2225669/86‑1 (MQ=255)
                                                                                  tCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCa                                              <  1:1725502/140‑1 (MQ=255)
                                                                                  tCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCa                                              <  2:316045/140‑1 (MQ=255)
                                                                                  tCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCa                                              <  2:1537202/140‑1 (MQ=255)
                                                                                   cTGTCAGCTATTGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCaa                                             <  1:2070218/140‑1 (MQ=255)
                                                                                   cTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCaa                                             >  2:814525/1‑140 (MQ=255)
                                                                                            aTGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGa                                    <  2:1091138/140‑1 (MQ=255)
                                                                                            aTGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGa                                    <  2:558087/140‑1 (MQ=255)
                                                                                            aTGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGa                                    <  1:2360096/140‑1 (MQ=255)
                                                                                                tGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGAt                                >  2:49959/1‑140 (MQ=255)
                                                                                                tGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTAACAATCATATGGACGAt                                >  2:471732/1‑140 (MQ=255)
                                                                                                  agaCTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGAt                                                                                             <  1:2375338/77‑1 (MQ=255)
                                                                                                  agaCTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGAt                                                                                             >  2:2375338/1‑77 (MQ=255)
                                                                                                    aCTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaa                                                           <  2:430327/109‑1 (MQ=255)
                                                                                                    aCTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGgaa                                                           >  1:430327/1‑109 (MQ=255)
                                                                                                    aCTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATtgtg                            <  1:1147168/140‑1 (MQ=255)
                                                                                                    aCTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATtgtg                            >  2:1357340/1‑140 (MQ=255)
                                                                                                    aCTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATtgtg                            >  1:773098/1‑140 (MQ=255)
                                                                                                    aCTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATtgtg                            >  1:1595846/1‑140 (MQ=255)
                                                                                                    aCTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATtgtg                            >  1:1237803/1‑140 (MQ=255)
                                                                                                    aCTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATtgtg                            <  1:78543/140‑1 (MQ=255)
                                                                                                    aCTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATtgtg                            <  1:293862/140‑1 (MQ=255)
                                                                                                        tACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAat                        >  1:1279943/1‑140 (MQ=255)
                                                                                                        tACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAat                        >  1:1734241/1‑140 (MQ=255)
                                                                                                           atatatCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAatgat                     <  2:983916/140‑1 (MQ=255)
                                                                                                              tatCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGAACGGCGACGGAGCTTGGGGATACAAAGGGACTCATTTATTTGCAGCCGGCTGGAACGGAAGTTTACCAATCATACAGACGAGTCTGGGatgatgga                  >  2:2024113/1‑138 (MQ=255)
                                                                                                              tatCTGAAAAGCAGCCGGCAACAATACCTGAAGTTTTATACAGAACGGCGACGGAGCTTGGGGATACAAAGGGAATAATTTATTTGCAGCCGGATGGAACTGAAGTTTACCAATCATACAGACGATTATGGGatgatgga                  >  2:1430205/1‑138 (MQ=255)
                                                                                                               atCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGACTCATTTATTTGCAGCCGGATGGACGTGAAGTGTACCAATCATATGGACGATTGTGGACTGAtgcgt                 >  2:1881094/1‑140 (MQ=255)
                                                                                                               atCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAATGAtgcgt                 >  1:2229066/1‑140 (MQ=255)
                                                                                                               atCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAATGAtgcgt                 >  2:1873911/1‑140 (MQ=255)
                                                                                                               atCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAATGAtgcgt                 >  2:934474/1‑140 (MQ=255)
                                                                                                                tCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAATGAtgcgtt                >  2:863010/1‑140 (MQ=255)
                                                                                                                tCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAATGAtgcgtt                >  1:1384007/1‑140 (MQ=255)
                                                                                                                  tGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAATGAtgcgttgc              >  2:2124095/1‑140 (MQ=255)
                                                                                                                  tGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAATGAtgcgttgc              >  2:2056373/1‑140 (MQ=255)
                                                                                                                    aaGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAATGAtgcgttgcgt            >  2:1008532/1‑140 (MQ=255)
                                                                                                                    aaGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAATGAtgcgttgcgt            >  2:445848/1‑140 (MQ=255)
                                                                                                                    aaGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAATGAtgcgttgcgt            >  1:1724232/1‑140 (MQ=255)
                                                                                                                    aaGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAATGAtgcgttgcgt            >  1:2024320/1‑140 (MQ=255)
                                                                                                                      gagCCGCCGGCAACGATACCTGAAGTTTTATACAGACCGGCTGCGGAGCTTGGGGATACACAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTCCCAATCATATGGACGATTGTGGAATGATGCGTTGCGTAt          <  2:682558/140‑1 (MQ=255)
                                                                                                                      gagCAGCCTGCAACGATACCTGACGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGGCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAATGATGCGTTGCGTAt          <  2:211867/140‑1 (MQ=255)
                                                                                                                         cagcCGTCAACGATACCTGTAGTTTTATACAGAACGTCTGCGGAGCTTGGGGATACTAACGGAATCATTTATTTGCAGCAGTATGGAAGTGATGTGTACCAATCATATGGACGATTGTGGCATGATGCGTGGCGTATCgg       >  2:2069559/1‑139 (MQ=255)
                                                                                                                         cagcCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGa                                                                >  2:241846/1‑83 (MQ=255)
                                                                                                                         cagcCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAatgat                     <  2:930074/126‑1 (MQ=255)
                                                                                                                         cagcCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAatgat                     >  1:930074/1‑126 (MQ=255)
                                                                                                                         cagcCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAATGATGCGTTGCGTATCGt       >  1:1920564/1‑140 (MQ=255)
                                                                                                                         cagcCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAATGATGCGTTGCGTATCGt       >  1:701930/1‑140 (MQ=255)
                                                                                                                         cagcCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAATGATGCGTTGCGTATCGt       >  1:466306/1‑140 (MQ=255)
                                                                                                                         cagcCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAATGATGCGTTGCGTATCGt       >  1:1645377/1‑140 (MQ=255)
                                                                                                                         cagcCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAATGATGCGTTGCGTATCGt       >  1:1920869/1‑140 (MQ=255)
                                                                                                                          agcCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAATGATGCGTTGCGTATCGTg      >  1:126528/1‑140 (MQ=255)
                                                                                                                          agcCGGCAACGATACCTGAAGTTTTATACAGAACGGATGCGGAGCTTGGGGATACAAAGGGACTCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCAGATGGACGATTGTGGAATGATGCGTTGCGTATCGTg      >  1:255275/1‑140 (MQ=255)
                                                                                                                            ccGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAATGATGCGTTGCGTATCGTGaa    <  1:2109310/140‑1 (MQ=255)
                                                                                                                              ggCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAATGATGCGTTGCGTATCGTGAAgg  >  1:538742/1‑140 (MQ=255)
                                                                                                                                    |                                                                                                                                     
GAGAATCTACATAAAAATTCGCAGGTGAAATGGGGGCAGCGCGATGAGAAATAATGATAATACCCGCAATTTAGCTAATCCTTCTGTCAGCTATGGTGAGACTTTACATATATCTGAAGAGCAGCCGGCAACGATACCTGAAGTTTTATACAGAACGGCTGCGGAGCTTGGGGATACAAAGGGAATCATTTATTTGCAGCCGGATGGAAGTGAAGTGTACCAATCATATGGACGATTGTGGAATGATGCGTTGCGTATCGTGAAGG  >  NZ_CP034943/1761449‑1761714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: