Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,846,125 G→A 5.5% G212G (GGG→GGA EO946_RS09265 → aminotransferase class I/II‑fold pyridoxal phosphate‑dependent enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,846,1250GA 5.5% 355.8 / 6.9 109G212G (GGG→GGAEO946_RS09265aminotransferase class I/II‑fold pyridoxal phosphate‑dependent enzyme
Reads supporting (aligned to +/- strand):  ref base G (35/68);  new base A (3/3);  total (38/71)
Fisher's exact test for biased strand distribution p-value = 4.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.86e-01

Only 100 of 125 total aligned reads displayed.

ACGAAAGTAATCGGCATTCAGCGCTCAAAAGGGTATGCAAATCGTCCTTCTTTCTTAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGAAAAGCGAAATGGATTGAAGCTTGCTCAT  >  NZ_CP034943/1845988‑1846264
                                                                                                                                         |                                                                                                                                           
aCGAAAGTAATCGGCATTCAGCGCTCAAAAGGGTATGCAAATCGTCCTTCTTTCTTAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGa                                                                                                                                           <  1:1287750/140‑1 (MQ=255)
         aTCGGCATTCAGCGCTCAAAAGGGTATGCAAATCGTCCTTCTTTCTTAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGa                                                                                                                                  <  2:483011/140‑1 (MQ=255)
          tCGGCATTCAGCGCTCAAAAGGGTATGCAAATCGTCCTTCTTTCTTAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGaa                                                                                                                                 <  1:2425156/140‑1 (MQ=255)
          tCGGCATTCAGCGCTCAAAAGGGTATGCAAATCGTCCTTCTTTCTTAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGaa                                                                                                                                 <  1:328392/140‑1 (MQ=255)
          tCGGCATTCAGCGCTCAAAAGGGTATGCAAATCGTCCTTCTTTCTTAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGaa                                                                                                                                 <  1:1257567/140‑1 (MQ=255)
          tCGGCATTCAGCGCTCAAAAGGGTATGCAAATCGTCCTTCTTTCTTAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGaa                                                                                                                                 <  1:966559/140‑1 (MQ=255)
          tCGGCATTCAGCGCTCAAAAGGGTATGCAAATCGTCCTTATTTCTTAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGaa                                                                                                                                 <  1:1591178/140‑1 (MQ=255)
          tCGGCATTCAGCGCTCAAAAGCGTATCCAAATCGTCCTTCTTTCTTAAATAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGaa                                                                                                                                 <  1:311906/140‑1 (MQ=255)
            ggCATTCAGCGCTCAAAAGGGTATGCAAATCGTCCTTCTTTCTTAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGACTTTGTGGAaga                                                                                                                               >  2:865814/1‑140 (MQ=255)
              cATTCAGCGCTCAAAAGGGTATGCAAATCGTCCTTCTTTCTTAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGc                                                                                                                             <  2:1215766/140‑1 (MQ=255)
               aTTCAGCGCTCAAAAGGGTATGCAAATCGTCCTTCTTTCTTAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCt                                                                                                                            <  1:1196891/140‑1 (MQ=255)
               aTTCAGCGCTCAAAAGGGTATGCAAATCGTCCTTCTTTCTTAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCt                                                                                                                            <  1:768905/140‑1 (MQ=255)
               aTTCAGCGCTCAAAAGGGTATGCAAATCGTCCTTCTTTCTTAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCt                                                                                                                            <  1:1679865/140‑1 (MQ=255)
               aTTCAGCGCTCAAAAGGGTATGCAAATCGTCCTTCTTTCTTAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTAGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCt                                                                                                                            <  1:642173/140‑1 (MQ=255)
               aTTCAGCGCTCAAAAGGGTATGCAAATCGTCCTTCTTTCATAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCt                                                                                                                            <  1:1791719/140‑1 (MQ=255)
                 tCAGCGCTCAAAAGGATATGCGAATCGCCCTTCTTTCTTAATTAGTGACATAAAAGAAATGATCCGTTTTGTAAAAGAAATCAATGAAAATCTGATCGTCTTTGTGGACAACTGTTACGGAGAATTTGTGGAAGAGCTTg                                                                                                                          >  2:781153/1‑140 (MQ=255)
                 tCAGCGCTCAAAAGGATATGCGAATCGCCCTTCTTTCTTAATTAGTGAAATAAAAGAAATGATCCGTTTTGTAAAAGAAATCAATGAAAATCTGATCGTCTTTGTGGACAACTGTTACGGAGAATTTGTGGAAGAGCTTg                                                                                                                          >  2:237882/1‑140 (MQ=255)
                    gcgcTCAAACGGGGAGGCAAATCGTCCGTCTTTCTTAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCCAGGAACACTGGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGc                                                                                                                       <  1:1946190/140‑1 (MQ=255)
                     cgcTCAAAAGGGTATGCAAATCGTCCTTCTTTCTTAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCTTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGcc                                                                                                                      >  1:1408577/1‑140 (MQ=255)
                     cgcTCAAAAGGGTATGCAAATCGTCCTTCTTTCTTAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGcc                                                                                                                      <  1:1083055/140‑1 (MQ=255)
                     cgcTCAAAAGGGTATGCAAATCGTCCTTCTTTCTTAATGAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAAGTTGAGCGTCTTTGTGGACAACGGGTCCGGGGAAGTTGTGGAAGAGCTTGCGcc                                                                                                                      >  1:1867813/1‑140 (MQ=255)
                     cgcACAAAAGGCTATGCAACTCGTCCTTCTTTCTTAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGcc                                                                                                                      <  1:1961992/140‑1 (MQ=255)
                          aaaaGGGTATGCAAATCGTCCTTCTTTCTTAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGcc                                                                                                                 <  1:455428/140‑1 (MQ=255)
                          aaaaGGGTATGCAAATCGTCCTTCTTTCTTAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGcc                                                                                                                 <  1:1273367/140‑1 (MQ=255)
                              gggTATGCAAATCGTCCTTCTTTCTTAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGt                                                                                                             <  1:1347271/140‑1 (MQ=255)
                                  aTGCAAATCGTCCTTCTTTCTTAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGa                                                                                                         >  1:2108452/1‑140 (MQ=255)
                                  aTGCAAATCGTCCTTCTTTCTTAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGa                                                                                                         >  2:1839770/1‑140 (MQ=255)
                                  aTGCAAATCGTCCTTCTTTCTTAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTGGTGGACAAGTGTTACGGGGACTTTGTGGCAGAGCTGGAGCCTTGCCATGTCGGa                                                                                                         >  1:1360212/1‑140 (MQ=255)
                                    gCAAATCGTCCTTCTTTCTTAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGc                                                                                                       >  1:1687854/1‑140 (MQ=255)
                                    gCAAATCGTCCTTCTTTCTTAATTAATGAAATAAAAGAAATGATACGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGCGTTGCCATGTCGGAGc                                                                                                       >  1:466527/1‑140 (MQ=255)
                                         tCGTCCTTCTTTCTTAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATc                                                                                                  >  1:1441869/1‑140 (MQ=255)
                                         tCGTCCTTCTTTCTTAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATc                                                                                                  >  1:2349333/1‑140 (MQ=255)
                                         tCGTCCTTCTTTCTTAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATc                                                                                                  >  1:2346521/1‑140 (MQ=255)
                                         tCGTCCTTCTTTCTTAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATc                                                                                                  >  2:2343905/1‑140 (MQ=255)
                                         tCGTCCTTCTTTCTTAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATc                                                                                                  >  2:2310997/1‑140 (MQ=255)
                                         tCGTCCTTCTTTCTTAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATc                                                                                                  >  1:1576508/1‑140 (MQ=255)
                                         tCGTCCTTCTTTCTTAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATc                                                                                                  >  2:1518630/1‑140 (MQ=255)
                                         tCGTCCTTCTTTCTTAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATc                                                                                                  >  1:1775164/1‑140 (MQ=255)
                                             ccTTCTTTCTTAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGAt                                                                                              <  1:1049768/140‑1 (MQ=255)
                                             ccTTCTTTCTTAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGAt                                                                                              <  2:1047337/140‑1 (MQ=255)
                                             ccTTCTTTCTTAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGAt                                                                                              <  1:535864/140‑1 (MQ=255)
                                                tctttcttAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGc                                                                                           <  1:146735/140‑1 (MQ=255)
                                                   ttcttAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCggg                                                                                        <  2:1419797/140‑1 (MQ=255)
                                                   ttcttAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCggg                                                                                        <  2:205911/140‑1 (MQ=255)
                                                       taattaatGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGAtct                                                                                    <  1:1455310/140‑1 (MQ=255)
                                                        aattaatGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGAtctc                                                                                   >  1:1160172/1‑140 (MQ=255)
                                                        aattaatGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGAGCTGATGGCGGGAtctc                                                                                   >  1:1215934/1‑140 (MQ=255)
                                                                           aTGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCcggcgg                                                                <  2:490377/140‑1 (MQ=255)
                                                                                             gAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACagg                                              <  1:1112745/140‑1 (MQ=255)
                                                                                             gAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACagg                                              <  2:27319/140‑1 (MQ=255)
                                                                                             gAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACagg                                              <  1:1112873/140‑1 (MQ=255)
                                                                                                aTCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACaggagg                                           >  2:543895/1‑140 (MQ=255)
                                                                                                aTCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATGTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCGCATTAAAAATCCAGGCTGCGGTCTTGCGAAAACtggagg                                           >  1:1331131/1‑140 (MQ=255)
                                                                                                   aaTGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTa                                        >  2:684544/1‑140 (MQ=255)
                                                                                                   aaTGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTa                                        >  2:1944980/1‑140 (MQ=255)
                                                                                                    aTGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTAt                                       <  1:1447256/140‑1 (MQ=255)
                                                                                                             tGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGa                              >  1:2358633/1‑140 (MQ=255)
                                                                                                             tGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGa                              >  1:129584/1‑140 (MQ=255)
                                                                                                                     tttGTGGACAACTGTTACGGAGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGACCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGCCTTGCGAAAACAGGCGGCTACCTCGTCGGAAAAGCGaa                      <  1:237882/140‑1 (MQ=255)
                                                                                                                     tttGTGGACAACTGTTACGGAGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGACCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGCCTTGCGAAAACAGGCGGCTACCTCGTCGGAAAAGCGaa                      <  1:781153/140‑1 (MQ=255)
                                                                                                                     tttGTGGACAACTGTTACGGAGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGACCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGCCTTGCGAAAACAGGCGGCTACCTCGTCGGAAAAGCGaa                      <  1:606129/140‑1 (MQ=255)
                                                                                                                      ttGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGAAAAGCGaaa                     >  2:1952272/1‑140 (MQ=255)
                                                                                                                          ggACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGAAAAGCGAAATGGa                 <  2:869007/140‑1 (MQ=255)
                                                                                                                          ggACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGAAAAGCGAAATGGa                 <  2:620479/140‑1 (MQ=255)
                                                                                                                               aCTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGAAAAGCGAAATGGATTGaa            >  2:666641/1‑140 (MQ=255)
                                                                                                                               aCTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGAAAAGCGAAATGGATTGaa            >  2:673536/1‑140 (MQ=255)
                                                                                                                               aCTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGAAAAGCGAAATGGATTGaa            >  2:1374433/1‑140 (MQ=255)
                                                                                                                                cTGTTACGGGTAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGAAAAGCGAAATGGATTGAAg           <  2:1829534/140‑1 (MQ=255)
                                                                                                                                cTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGAAAAGCGAAATGGATTGAAg           <  1:1127350/140‑1 (MQ=255)
                                                                                                                                cTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGAAAAGCGAAATGGATTGAAg           <  2:896112/140‑1 (MQ=255)
                                                                                                                                cTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGAAAAGCGAAATGGATTGAAg           <  2:2408041/140‑1 (MQ=255)
                                                                                                                                cTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGAAAAGCGAAATGGATTGAAg           <  2:270364/140‑1 (MQ=255)
                                                                                                                                cTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGAAAAGCGAAATGGATTGAAg           <  2:322858/140‑1 (MQ=255)
                                                                                                                                cTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGAAAAGCGAAATGGATTGAAg           <  1:1127282/140‑1 (MQ=255)
                                                                                                                                cTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGAAAAGCGAAATGGATTGAAg           <  1:1623165/140‑1 (MQ=255)
                                                                                                                                cTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGAAAAGCGAAATGGATTGAAg           <  1:1735276/140‑1 (MQ=255)
                                                                                                                                cTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGAAAAGCGAAATGGATTGAAg           <  1:1153702/140‑1 (MQ=255)
                                                                                                                                cTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGAAAAGCGAAATGGATTGAAg           <  1:2129356/140‑1 (MQ=255)
                                                                                                                                cTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGAAAAGCGAAATGGATTGAAg           <  2:1122313/140‑1 (MQ=255)
                                                                                                                                cTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGAAAAGCGAAATGGATTGAAg           <  1:412558/140‑1 (MQ=255)
                                                                                                                                cTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGAAAAGCGAAATGGATTGAAg           <  1:627483/140‑1 (MQ=255)
                                                                                                                                cTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGAAAAGCGAAATGGATTGAAg           <  1:626616/140‑1 (MQ=255)
                                                                                                                                cTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGAAAAGCGAAATGGATTGAAg           <  1:543895/140‑1 (MQ=255)
                                                                                                                                cTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCGTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGAAAAGCGAAATGGATTGAAg           <  2:1932075/140‑1 (MQ=255)
                                                                                                                                  gTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGAAAAGCGAAATGGATTGAAGCt         <  1:1375888/140‑1 (MQ=255)
                                                                                                                                        gggACTTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGAAAAGCGAAATGGATTGAAGCTTGCTCa   <  2:1576508/140‑1 (MQ=255)
                                                                                                                                        gggAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGc                                                                                                       >  2:1246013/1‑40 (MQ=255)
                                                                                                                                        gggAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGc                                                                                                       <  1:1246013/40‑1 (MQ=255)
                                                                                                                                        gggAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGAAAAGCGAAATGGATTGAAGCTTGCTCa   <  1:268854/140‑1 (MQ=255)
                                                                                                                                        gggAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGAAAAGCGAAATGGATTGAAGCTTGCTCa   <  1:1581512/140‑1 (MQ=255)
                                                                                                                                        gggAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGAAAAGCGAAATGGATTGAAGCTTGCTCa   <  1:2345314/140‑1 (MQ=255)
                                                                                                                                        gggAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGAAAAGCGAAATGGATTGAAGCTTGCTCa   <  1:2218032/140‑1 (MQ=255)
                                                                                                                                        gggAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGAAAAGCGAAATGGATTGAAGCTTGCTCa   <  1:1259474/140‑1 (MQ=255)
                                                                                                                                        gggAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGAAAAGCGAAATGGATTGAAGCTTGCTCa   <  2:2349333/140‑1 (MQ=255)
                                                                                                                                        gggAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGAAAAGCGAAATGGATTGAAGCTTGCTCa   <  2:2346521/140‑1 (MQ=255)
                                                                                                                                        gggAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGAAAAGCGAAATGGATTGAAGCTTGCTCa   <  2:1441869/140‑1 (MQ=255)
                                                                                                                                        gggAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGAAAAGCGAAATGGATTGAAGCTTGCTCa   <  2:1775164/140‑1 (MQ=255)
                                                                                                                                        gggAATTTGTGGAAGAGCTTGAGCCTTGACATGTCGGAGCCGATCTGATGGAGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGAAAAGCGAAATGGATTGAAGCTTGCTCa   <  1:976637/140‑1 (MQ=255)
                                                                                                                                         ggAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGAACAGCGAAATGGATTGAAGCTTGCTCAt  <  2:1791309/140‑1 (MQ=255)
                                                                                                                                         ggAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGAAAAGCGAAATGGATTGAAGCTTGCTCAt  <  2:136398/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                           
ACGAAAGTAATCGGCATTCAGCGCTCAAAAGGGTATGCAAATCGTCCTTCTTTCTTAATTAATGAAATAAAAGAAATGATCCGGTTTGTAAAAGAAATCAATGAAAACTTGATCGTCTTTGTGGACAACTGTTACGGGGAATTTGTGGAAGAGCTTGAGCCTTGCCATGTCGGAGCCGATCTGATGGCGGGATCTCTCATTAAAAATCCCGGCGGCGGTCTTGCGAAAACAGGAGGCTATCTCGTTGGAAAAGCGAAATGGATTGAAGCTTGCTCAT  >  NZ_CP034943/1845988‑1846264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: