Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 344,599 G→A 5.1% K149K (AAG→AAA putR → proline utilization transcriptional regulator PutR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943344,5990GA 5.1% 442.0 / 8.8 136K149K (AAG→AAAputRproline utilization transcriptional regulator PutR
Reads supporting (aligned to +/- strand):  ref base G (68/61);  new base A (4/3);  total (72/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 137 total aligned reads displayed.

GTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACACCGTGCTGCCTTCCATGTTTTCGGTTGTCA  >  NZ_CP034943/344460‑344729
                                                                                                                                           |                                                                                                                                  
gTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAg                                                                                                                                    <  1:91775/140‑1 (MQ=255)
             aGGGGAAGTCATGGGCTACATCTGGATTCAGGAGCTGGACCAAAATCTGACGGATGAAGAGCTCGACTTTTTGTACGAGACCTCCTTCCATGTCGGGAAAATCATTTATAAAACGAATAAATTGAAACAGGAAAAGgaag                                                                                                                       >  1:1278619/1‑140 (MQ=255)
             aGGGGAAGTCATGGGCTACATCTGGATTCAGGAGCTGGACCAAAATCTGACGGATGAAGAGCTCGACTTTTTGTACGAGACCTCCTTCCATGTCGGGAAAATCATTTATAAAACGAATAAATTGAAACAGGAAAAGgaag                                                                                                                       >  1:1278980/1‑140 (MQ=255)
             aGGGGAAGTCATGGGCTACATCTGGATTCAGGAGCTGGACCAAAATCTGACGGATGAAGAGCTCGACTTTTTGTACGAGACCTCCTTCCATGTCGGGAAAATCATTTATAAAACGAATAAATTGAAACAGGAAAAGgaag                                                                                                                       >  1:512300/1‑140 (MQ=255)
              ggggAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATTTGGGTAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGgaaga                                                                                                                      <  1:332783/140‑1 (MQ=255)
                 gAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGaaaa                                                                                                                   >  1:1212449/1‑140 (MQ=255)
                  aaGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGaaaaa                                                                                                                  >  1:468743/1‑140 (MQ=255)
                  aaGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGaaaaa                                                                                                                  >  1:2160852/1‑140 (MQ=255)
                  aaGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGaaaaa                                                                                                                  >  1:1352220/1‑140 (MQ=255)
                  aaGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGaaaaa                                                                                                                  >  1:2087031/1‑140 (MQ=255)
                  aaGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCAGTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGaaaaa                                                                                                                  >  1:1553304/1‑140 (MQ=255)
                   aGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAg                                                                                                                 >  2:1981343/1‑140 (MQ=255)
                   aGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAg                                                                                                                 >  2:508645/1‑140 (MQ=255)
                      cATGGGCTACATCTTGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGTACTGGTCTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCTTTTATAAAACGACCAAGCTGAAGCAGGAAAAGGTAGAAAAAGCgg                                                                                                              <  2:1653395/140‑1 (MQ=255)
                         gggCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGCAAAGGAAGAAAAAGCGGAAg                                                                                                           >  2:301548/1‑140 (MQ=255)
                         gggCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAg                                                                                                           >  2:129322/1‑140 (MQ=255)
                           gatACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGAt                                                                                                         <  2:2237701/138‑1 (MQ=255)
                           gCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGAt                                                                                                         <  2:2398903/140‑1 (MQ=255)
                           gCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGAt                                                                                                         <  1:1648870/140‑1 (MQ=255)
                           gCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGAt                                                                                                         <  2:1422390/140‑1 (MQ=255)
                              aCATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGAtctc                                                                                                      >  1:1353455/1‑140 (MQ=255)
                              aCATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGAtctc                                                                                                      >  1:2306252/1‑140 (MQ=255)
                               cATCTGGGTTCAGGAGCTGGAGCAAAATCTGACGGAAGAAGTACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAATTCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGAGCTCa                                                                                                     >  1:1653395/1‑140 (MQ=255)
                               cATCTGGGTTCAGGAGCTGGACCCAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGCAAAGGAAGAAAAAGCGGAAGATCTCa                                                                                                     >  2:1635291/1‑140 (MQ=255)
                               cATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGACAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGACGATCTCa                                                                                                     >  2:1517513/1‑140 (MQ=255)
                               cATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCgg                                                                                                              <  2:141672/131‑1 (MQ=255)
                               cATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCgg                                                                                                              >  1:141672/1‑131 (MQ=255)
                               cATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCa                                                                                                     >  2:804740/1‑140 (MQ=255)
                               cATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCa                                                                                                     >  2:166829/1‑140 (MQ=255)
                               cATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCa                                                                                                     >  2:1754820/1‑140 (MQ=255)
                               cATCTGGGTTCAGGACCTGGACCAACATCTGACGGACGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCTTGTCGGGAAAATCATTTATAAAACGAACAAGCGGCCGCAGGCAAAGGCCGAAAAAGCGGAACATCTCa                                                                                                     >  2:1310169/1‑140 (MQ=255)
                                    gggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTaaa                                                                                                <  1:588675/140‑1 (MQ=255)
                                    gggTTCAGGAGCTGGAACAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTaaa                                                                                                <  1:226394/140‑1 (MQ=255)
                                     ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGACAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAAc                                                                                               >  2:2323991/1‑140 (MQ=255)
                                     ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGaaa                                                                                                                    <  1:1298617/119‑1 (MQ=255)
                                     ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAAc                                                                                               >  2:199675/1‑140 (MQ=255)
                                     ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAAc                                                                                               >  2:2049134/1‑140 (MQ=255)
                                     ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAAc                                                                                               >  2:1482957/1‑140 (MQ=255)
                                     ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAACGAAGAAAAAGCGGAAGATCACATTAAAc                                                                                               >  2:416637/1‑140 (MQ=255)
                                     ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGGCGGGAAAATCATTGATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAAc                                                                                               >  2:1162843/1‑140 (MQ=255)
                                        tCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACggg                                                                                            <  1:257015/140‑1 (MQ=255)
                                        tCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACggg                                                                                            <  2:2182117/140‑1 (MQ=255)
                                         cAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGc                                                                                           <  2:1086800/140‑1 (MQ=255)
                                          aGGAGCTGGACCAAAATCTGACGGATGAAGAGCTCGACTTTTTGTACGAGACCTCCTTCCATGTCGGGAAAATCATTTATAAAACGAATAAATTGAAACAGGAAAAGGAAGAAAAAGCAGAGGATCTCATCAAACGGTCg                                                                                          >  2:157467/1‑140 (MQ=255)
                                          aGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGACAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCg                                                                                          >  1:1489631/1‑140 (MQ=255)
                                          aGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGc                                                                                           >  2:1898390/1‑139 (MQ=255)
                                          aGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGc                                                                                           <  1:1898390/139‑1 (MQ=255)
                                          aGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCg                                                                                          >  1:2186428/1‑140 (MQ=255)
                                          aGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCg                                                                                          >  2:455489/1‑140 (MQ=255)
                                          aGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCg                                                                                          >  1:1185537/1‑140 (MQ=255)
                                          aGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCACGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCg                                                                                          >  2:2378229/1‑140 (MQ=255)
                                           ggAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGa                                                                                         <  2:1943849/140‑1 (MQ=255)
                                           ggAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGa                                                                                         <  2:1943809/140‑1 (MQ=255)
                                              gCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCt                                                                                      <  2:1185537/140‑1 (MQ=255)
                                               cTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTa                                                                                     >  2:1698153/1‑140 (MQ=255)
                                                tGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTAt                                                                                    <  2:1175369/140‑1 (MQ=255)
                                                 ggACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATc                                                                                   >  1:781061/1‑140 (MQ=255)
                                                 ggACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATc                                                                                   >  1:2111707/1‑140 (MQ=255)
                                                 ggACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGAAGGAAAAGGAAGAAAAAGCGGTAGATCTCATTAAAAGGGAGAtctctc                                                                                   >  2:2035925/1‑140 (MQ=255)
                                                   aCCAAACTCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAg                                                                                 <  2:105420/140‑1 (MQ=255)
                                                    ccAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGc                                                                                <  1:1365925/140‑1 (MQ=255)
                                                       aaaTCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTTTCAGCAAt                                                                             >  2:1870211/1‑140 (MQ=255)
                                                       aaaTCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAAt                                                                             >  2:13114/1‑140 (MQ=255)
                                                       aaaTCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAAt                                                                             >  2:1730250/1‑140 (MQ=255)
                                                          tCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTa                                                                          <  2:1575945/140‑1 (MQ=255)
                                                          tCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTa                                                                          <  1:1244396/140‑1 (MQ=255)
                                                           cTGACGGAAGCAGAACTCGCCTTTTTGTATGAGACCTCTTTTCATGTCGGGAACATCATGTATAAAACGAACACGCTGAAGCAGTAAAAGGAAGACACAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTAc                                                                         <  2:1447331/140‑1 (MQ=255)
                                                             gACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCa                                                                       >  2:832963/1‑140 (MQ=255)
                                                              aCGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCAc                                                                      <  1:1443870/140‑1 (MQ=255)
                                                               cGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACt                                                                     <  1:455489/140‑1 (MQ=255)
                                                                ggAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTg                                                                    <  2:1454403/140‑1 (MQ=255)
                                                                        aaCTCGACTTTTTGTATGAGACCTATTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGaa                                                            <  1:2402241/140‑1 (MQ=255)
                                                                                  tttGTACGAGACCTCCTTCCATGTCGGGAAAATCATTTATAAAACGAATAAATTGAAACAGGAAAAGGAAGAAAAAGCAGAGGATCTCATCAAACGGGCGATCTATCAGCAATTTACCTCTGAGAAGGAACTCAGACggg                                                  <  2:512300/140‑1 (MQ=255)
                                                                                  tttGTACGAGACCTCCTTCCATGTCGGGAAAATCATTTATAAAACGAATAAATTGAAACAGGAAAAGGAAGAAAAAGCAGAGGATCTCATCAAACGGGCGATCTATCAGCAATTTACCTCTGAGAAGGAACTCAGACggg                                                  <  2:1278980/140‑1 (MQ=255)
                                                                                  tttGTACGAGACCTCCTTCCATGTCGGGAAAATCATTTATAAAACGAATAAATTGAAACAGGAAAAGGAAGAAAAAGCAGAGGATCTCATCAAACGGGCGATCTATCAGCAATTTACCTCTGAGAAGGAACTCAGACggg                                                  <  2:1278619/140‑1 (MQ=255)
                                                                                         gagaCCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGa                                           >  2:804530/1‑140 (MQ=255)
                                                                                         gagaCCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGa                                           >  2:233247/1‑140 (MQ=255)
                                                                                          agaCCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCTGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGaa                                          <  1:3050/140‑1 (MQ=255)
                                                                                          agaCCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGaa                                          <  1:1780566/140‑1 (MQ=255)
                                                                                          agaCCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGaa                                          <  1:804740/140‑1 (MQ=255)
                                                                                            aCCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAg                                        >  2:963355/1‑140 (MQ=255)
                                                                                            aCCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAg                                        >  2:963105/1‑140 (MQ=255)
                                                                                                   ttCATGTCGGGAAAATCAGTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCAATGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAac                                 >  2:2414198/1‑140 (MQ=255)
                                                                                                    tCATTTCGTGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAaca                                <  2:1331248/140‑1 (MQ=255)
                                                                                                    tCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAaca                                <  1:832963/140‑1 (MQ=255)
                                                                                                    tCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAaca                                <  1:963105/140‑1 (MQ=255)
                                                                                                    tCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAaca                                <  1:963355/140‑1 (MQ=255)
                                                                                                    tCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAaca                                <  2:631160/140‑1 (MQ=255)
                                                                                                        gTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGACGCCGAAAGAATCAACACCGt                            >  1:1968478/1‑140 (MQ=255)
                                                                                                          cGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAACAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACACCGtgc                          <  1:1299544/140‑1 (MQ=255)
                                                                                                          cGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACACCGtgc                          <  1:1299622/140‑1 (MQ=255)
                                                                                                           gggAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACACCGtgct                         >  2:752874/1‑140 (MQ=255)
                                                                                                                  tCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTACACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGACTCAACACCGTGCTGCCTTcc                  <  1:260455/140‑1 (MQ=255)
                                                                                                                          aaaaCGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACACCGTGCTGCCTTCCATGTTTTc          <  2:1457787/140‑1 (MQ=255)
                                                                                                                            aaCGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACACCGTGCTGCCTTCCATGTTTTCgg        >  1:2263217/1‑140 (MQ=255)
                                                                                                                            aaCGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACACCGTGCTGCCTTCCATGTTTTCgg        <  2:2186428/140‑1 (MQ=255)
                                                                                                                            aaCGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACACCGTGCTGCCTTCCATGTGTTCgg        >  1:1458534/1‑140 (MQ=255)
                                                                                                                               gAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACACCGTGCTGCCTTCCATGTTTTCGGTTg     <  1:1482957/140‑1 (MQ=255)
                                                                                                                               gAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACACCGTGCTGCCTTCCATGTTTTCGGTTg     <  2:147326/140‑1 (MQ=255)
                                                                                                                                  cAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACACCGTGCTGCCTTCCATGTTTTCGGTTGTCa  <  2:716562/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                  
GTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACACCGTGCTGCCTTCCATGTTTTCGGTTGTCA  >  NZ_CP034943/344460‑344729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: