Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,018,250 A→T 5.0% I271I (ATT→ATA proC ← pyrroline‑5‑carboxylate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,018,2500AT 5.0% 374.2 / 7.1 110I271I (ATT→ATAproCpyrroline‑5‑carboxylate reductase
Reads supporting (aligned to +/- strand):  ref base A (52/52);  new base T (3/3);  total (55/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

TGTTAATGAACTGCTTCCAATCTTCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGACCTCAGGCTGTTCTCCTGTTTCCATGAGCATTTTTGCGGCAC  >  NZ_CP034943/2018113‑2018389
                                                                                                                                         |                                                                                                                                           
tGTTAATGAACTGCTTCCAATCTTCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAAt                                                                                                                                           >  1:1211726/1‑140 (MQ=255)
tGTTAATGAACTGCTTCCAATCTTCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAAt                                                                                                                                           >  1:2139808/1‑140 (MQ=255)
tGTTAATGAACTGCTTCCAATCTTCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAAt                                                                                                                                           <  2:1895803/140‑1 (MQ=255)
 gTTAATGAACTGCTTCCAATCTTCACAACGAGTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATc                                                                                                                                          >  2:24935/1‑140 (MQ=255)
 gTTAATGAACTGCTTCCAATCTTCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATc                                                                                                                                          >  2:25054/1‑140 (MQ=255)
 gTTAATGAACTGCTTCCAATCTTCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATc                                                                                                                                          >  2:1391991/1‑140 (MQ=255)
  ttAATGAACTGCTTCCAATCTTCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTAGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCg                                                                                                                                         >  2:1810450/1‑140 (MQ=255)
    aaTGAACTGCTTCCAATCTTCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCt                                                                                                                                       <  2:1229277/140‑1 (MQ=255)
       gAACTGCTTCCAATCTTCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCttta                                                                                                                                    >  1:1397909/1‑138 (MQ=255)
            gCTTCCAATCTTCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAAt                                                                                                                               >  1:2149008/1‑140 (MQ=255)
            gCTTCCAATCTTCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAAt                                                                                                                               >  1:986088/1‑140 (MQ=255)
            gCTTCCAATCTTCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCCTCGCTGATTTCCTTTTAAAGCTTAGCCGCATGATTAATCCCTTGAGAAAt                                                                                                                               >  1:305429/1‑140 (MQ=255)
             cTTCCAATCTTCACAACGACTCTTTTCATGCTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGCGCTGCAGTTTTTTCGATATCTTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTTATTGCTTGAGAAATc                                                                                                                              >  1:1770612/1‑140 (MQ=255)
             cTTCCAATCTTCACAACGACTCTTTTCATGCTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGCGCTGCAGTTTTTTCGATATCTTAGCTGATTTCCATTGAACGCATAGCCGCATGCTTTATTGCTTGAGAAATc                                                                                                                              >  1:1770702/1‑140 (MQ=255)
                  aaTCTTCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAACTCGCTtc                                                                                                                         >  1:1397227/1‑140 (MQ=255)
                  aaTCTTCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTtc                                                                                                                         >  2:692276/1‑140 (MQ=255)
                  aaTCTTCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTtc                                                                                                                         >  2:738649/1‑140 (MQ=255)
                  aaTCTTCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTtc                                                                                                                         >  1:570579/1‑140 (MQ=255)
                  aaTCTTCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTtc                                                                                                                         >  1:1475138/1‑140 (MQ=255)
                  aaTCTTCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTtc                                                                                                                         >  1:2379232/1‑140 (MQ=255)
                  aaTCTTCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCGTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTtc                                                                                                                         >  1:825746/1‑140 (MQ=255)
                       tCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTGGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTacgc                                                                                                                    >  2:1638923/1‑140 (MQ=255)
                       tCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGTACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTacgc                                                                                                                    >  1:2264636/1‑140 (MQ=255)
                       tCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTccgc                                                                                                                    >  2:2293762/1‑140 (MQ=255)
                       tCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTccgc                                                                                                                    >  2:2296008/1‑140 (MQ=255)
                       tCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTccgc                                                                                                                    >  1:758545/1‑140 (MQ=255)
                       tCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTccgc                                                                                                                    >  2:837090/1‑140 (MQ=255)
                              gACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGct                                                                                                             <  1:45569/140‑1 (MQ=255)
                                ctctTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGctct                                                                                                           >  1:1493839/1‑140 (MQ=255)
                                     ttCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTc                                                                                                      >  1:2248772/1‑140 (MQ=255)
                                     ttCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTc                                                                                                      <  1:1995833/140‑1 (MQ=255)
                                             tGTATCAGGCGTCACTTTCTCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGGCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTg                                                                                              <  2:1147786/140‑1 (MQ=255)
                                             tGTATCAGGCGTCACTTTATCACTCCTGATAGCCGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTACTTTGAACGCTTATCCGCAATCTTAATAGCTTTAGAAAAAGATTCAACGCCGCCGCTCATTTTAAATGCTTg                                                                                              >  1:952670/1‑140 (MQ=255)
                                             ggTATCAGGCGTCACTGTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGAGATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCGTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTg                                                                                              <  2:2214604/139‑1 (MQ=255)
                                                aTCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAg                                                                                           <  2:1416370/140‑1 (MQ=255)
                                                aTCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAg                                                                                           <  2:2204814/140‑1 (MQ=255)
                                                aTCAGGCGTCACTTTATCACTCCTGATAGCGGGGCCGCGGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAg                                                                                           <  2:2317540/140‑1 (MQ=255)
                                                  cAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGcc                                                                                         <  1:2325655/140‑1 (MQ=255)
                                                  cAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGcc                                                                                         <  1:947941/140‑1 (MQ=255)
                                                  cAGGCGTCACTTTATCACTCCTGATAGCGGGGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGcc                                                                                         <  1:1599705/140‑1 (MQ=255)
                                                    ggCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCg                                                                                       >  2:413299/1‑140 (MQ=255)
                                                    ggCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCg                                                                                       >  2:413288/1‑140 (MQ=255)
                                                         cACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCGTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGgcagc                                                                                  >  1:1686859/1‑140 (MQ=255)
                                                          aCTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGATCTTTTTCTATTATTGCAGCCCGgcagca                                                                                 <  2:2139808/140‑1 (MQ=255)
                                                           cTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGAGTTCCTTTGAACGCTTAGCCGCATGCGTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGcagcag                                                                                <  2:1919760/140‑1 (MQ=255)
                                                               aTCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCCTGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTcgt                                                                            >  2:1138409/1‑140 (MQ=255)
                                                               aTCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTcgt                                                                            >  2:312078/1‑140 (MQ=255)
                                                               aTCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTcgt                                                                            >  2:1900003/1‑140 (MQ=255)
                                                                        gATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCCTGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCgg                                                                   <  2:76660/140‑1 (MQ=255)
                                                                        gATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCgg                                                                   <  1:1411996/140‑1 (MQ=255)
                                                                         aTAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTTAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTACGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGGTCggg                                                                  >  2:1173041/1‑139 (MQ=255)
                                                                         aTAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGt                                                                  >  2:1961232/1‑140 (MQ=255)
                                                                          tAGCGGTTCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTg                                                                 <  2:286166/140‑1 (MQ=255)
                                                                          tAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTg                                                                 <  2:1312163/140‑1 (MQ=255)
                                                                           aGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGa                                                                >  2:134779/1‑140 (MQ=255)
                                                                              ggTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGt                                                             >  2:683191/1‑140 (MQ=255)
                                                                              ggTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTATCCGCCGCCGCTCTTTTTCAAGGCTTTCAGCCCGGCAGCAGTCGTTGCGTTCGGTGACGt                                                             >  1:1914238/1‑140 (MQ=255)
                                                                                tGCCGCTGTTTTTTCGATATCGTCGCTGATTTGCTTTTAACGCTTAGCCGCAAGCTTAATCCCTTGAGAAATAGCGTCTCCGCCGCCGCTCTTTTTAAAGGCGTGGAGCCCGGCAGCAGTCGTTACGTTCGGTGACGTGa                                                           >  1:1378907/1‑140 (MQ=255)
                                                                                tGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGTAAGCTTAGCCCCATGCTTAATAGCTTGAGAAATCGCTTCTCCCCCGCCGCTCTTTTTCAAAGCATTCAGCCCGGCAGCATTCGTTCCGTTCGGTGACGTGa                                                           >  1:1312163/1‑140 (MQ=255)
                                                                                tGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAACTCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGa                                                           >  1:1774092/1‑140 (MQ=255)
                                                                                tGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTg                                                                 >  1:1735980/1‑134 (MQ=255)
                                                                                tGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGa                                                           >  2:2118846/1‑140 (MQ=255)
                                                                                     cTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTg                                                      <  1:1349954/140‑1 (MQ=255)
                                                                                     cTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTg                                                      <  1:256436/140‑1 (MQ=255)
                                                                                     cTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTg                                                      <  2:736121/140‑1 (MQ=255)
                                                                                     cTGTTTTTTCGATATCGACGCTGATGTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTg                                                      <  2:1606007/140‑1 (MQ=255)
                                                                                      tGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATAgtgt                                                     <  2:621389/140‑1 (MQ=255)
                                                                                      tGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGt                                                     <  2:1586947/140‑1 (MQ=255)
                                                                                      tGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGt                                                     <  2:2334607/140‑1 (MQ=255)
                                                                                      tGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGt                                                     <  2:1134530/140‑1 (MQ=255)
                                                                                      tGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTGAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGt                                                     <  2:376127/140‑1 (MQ=255)
                                                                                           tttCGATATCTTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTTATTGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCCGCTGCTGTCGTTCCGTTCGGTGACGTGATATTATCTCtt                                                <  2:1770702/140‑1 (MQ=255)
                                                                                           tttCGATATCTTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTTATTGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCCGCTGCTGTCGTTCCGTTCGGTGACGTGATATTATCTCtt                                                <  2:1770791/140‑1 (MQ=255)
                                                                                           tttCGATATCTTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTTATTGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCCGCTGCTGTCGTTCCGTTCGGTGACGTGATATTATCTCtt                                                <  2:1770612/140‑1 (MQ=255)
                                                                                            ttCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTa                                               <  1:2134598/140‑1 (MQ=255)
                                                                                            ttCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTa                                               <  1:60414/140‑1 (MQ=255)
                                                                                            ttCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTa                                               <  1:1764634/140‑1 (MQ=255)
                                                                                            ttCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTa                                               <  2:2345764/140‑1 (MQ=255)
                                                                                                atatCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTcgtt                                                                           <  2:748094/108‑1 (MQ=255)
                                                                                                atatCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTcgtt                                                                           >  1:748094/1‑108 (MQ=255)
                                                                                                 tatCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGAcc                                          <  1:2037370/140‑1 (MQ=255)
                                                                                                   tcgtcgCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGACATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGACCTc                                        <  2:2248772/140‑1 (MQ=255)
                                                                                                      tcgCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGACCTCAgg                                     <  1:2293762/140‑1 (MQ=255)
                                                                                                         cTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGACCTCAGGCTg                                  >  2:2132982/1‑140 (MQ=255)
                                                                                                              ttCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGACCTCAGGCTGTtctc                             <  2:348427/140‑1 (MQ=255)
                                                                                                                           ttAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTa                                               <  1:2118143/109‑1 (MQ=255)
                                                                                                                           ttAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTa                                               >  2:2118143/1‑109 (MQ=255)
                                                                                                                                     gCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGACCTCAGGCTGTTCTCCTGTTTCCATGAGCATTTTTGCg      <  1:1909182/140‑1 (MQ=255)
                                                                                                                                     gCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGACCTCAGGCTGTTCTCCTGTTTCCATGAGCATTTTTGCg      <  1:2132982/140‑1 (MQ=255)
                                                                                                                                     gCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGACCTCAGGCTGTTCTCCTGTTTCCATGAGCATTTTTGCg      <  1:683191/140‑1 (MQ=255)
                                                                                                                                     gCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGACCTCAGGCTGTTCTCCTGTTTCCATGAGCATTTTTGCg      <  2:2358275/140‑1 (MQ=255)
                                                                                                                                     gCTTAATCGCGTGAGAAATCGCTGCTCCGCCGCCGCTCTTTGTCCAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGACCTCAGGCTGTTCTCCTGTTTCCATGAGCATTTTTGCg      <  2:600510/140‑1 (MQ=255)
                                                                                                                                      cTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGACCTCAGGCTGTTCTCCTGTTTCCATGAGCATTTTTGCgg     <  1:312078/140‑1 (MQ=255)
                                                                                                                                      cTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGACCTCAGGCTGTTCTCCTGTTTCCATGAGCATTTTTGCgg     <  1:1900003/140‑1 (MQ=255)
                                                                                                                                      cTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGACCTCAGGCTGTTCTCCTGTTTCCATGAGCATTTTTGCgg     <  1:1138409/140‑1 (MQ=255)
                                                                                                                                       ttAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGACCTCAGGCTGTTCTCCTGTTTCCATGAGCATTTTTGCGGc    <  1:1814223/140‑1 (MQ=255)
                                                                                                                                       ctAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGACCTCAGGCTGTTCTCCTGTTTCCATGAGCATTTTTGCGGc    <  1:837090/139‑1 (MQ=255)
                                                                                                                                         aaTCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGACCTCAGGCTGTTCTCCTGTTTCCATGAGCATTTTTGCGGCAc  >  1:543879/1‑140 (MQ=255)
                                                                                                                                         aaTCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGACCTCAGGCTGTTCTCCTGTTTAAATGAGCATTTTTGCGGCAc  >  2:118611/1‑140 (MQ=255)
                                                                                                                                         aaTCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGACCGCAGGCGGTTCTCCTGTTTCCATGAGCCTTTTTGCGGCAc  >  1:987792/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                           
TGTTAATGAACTGCTTCCAATCTTCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGACCTCAGGCTGTTCTCCTGTTTCCATGAGCATTTTTGCGGCAC  >  NZ_CP034943/2018113‑2018389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: